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1.
Phytopathology ; 114(9): 2064-2070, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38857059

RESUMO

Mixtures of fungicides with different modes of action are commonly used as disease and resistance management tools, but little is known of mixtures of natural and synthetic products. In this study, mixtures of metabolites from the rhizobacterium Pseudomonas chlororaphis strain ASF009 formulated as Howler EVO with below-label rates (50 µg/ml) of conventional sterol demethylation inhibitor (DMI) fungicides were investigated for control of anthracnose of cherry (Prunus avium) caused by Colletotrichum siamense. Howler mixed with metconazole or propiconazole synergistically reduced disease severity through lesion growth. Real-time PCR showed that difenoconazole, flutriafol, metconazole, and propiconazole induced the expression of DMI target genes CsCYP51A and CsCYP51B in C. siamense. The addition of Howler completely suppressed the DMI fungicide-induced expression of both CYP51 genes. We hypothesize that the downregulation of DMI fungicide-induced expression of the DMI target genes may, at least in part, explain the synergism observed in detached fruit assays.


Assuntos
Colletotrichum , Fungicidas Industriais , Doenças das Plantas , Pseudomonas chlororaphis , Triazóis , Fungicidas Industriais/farmacologia , Triazóis/farmacologia , Colletotrichum/efeitos dos fármacos , Colletotrichum/fisiologia , Doenças das Plantas/microbiologia , Pseudomonas chlororaphis/genética , Pseudomonas chlororaphis/efeitos dos fármacos , Sinergismo Farmacológico , Regulação Fúngica da Expressão Gênica/efeitos dos fármacos , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Esterol 14-Desmetilase/genética , Esterol 14-Desmetilase/metabolismo
2.
Pestic Biochem Physiol ; 194: 105472, 2023 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-37532311

RESUMO

Conventional fungicides are used in IPM programs to manage fungal plant pathogens, but there are concerns about resistance development in target organisms, environmental contamination, and human health risks. This study explored the potential of calcium propionate (CaP), a common food preservative generally recognized as safe (GRAS) to control fungicide-resistant plant pathogens, mainly Botrytis cinerea, and botrytis blight in ornamentals. In-vitro experiments using mycelium growth inhibition indicated a mean EC50 value for CaP (pH 6.0) of 527 mg/L for six isolates of Botrytis cinerea as well as 618, 1354, and 1310 mg/L for six isolates each of Monilinia fructicola, Alternaria alternata, and Colletotrichum acutatum. In vitro efficacy tests indicated CaP equally inhibited mycelium growth of fungal isolates sensitive and resistant to FRAC codes 1, 2, 3, 7, 9, 11, 12, and 17 fungicides. CaP at 0.1% (pH 6.0-6.5) reduced infection cushion (IC) formation in vitro, botrytis blight on petunia flowers, and botrytis blight of cut flower roses with little to no visible phytotoxicity. Although higher concentrations strongly inhibited infection cushion formation, they did not improve efficacy and exhibited phytotoxicity. We hypothesize that high concentrations may create tissue damage that facilitates direct fungal penetration without the need for infection cushion and subsequent appressoria formation. This study indicates the potential usefulness of CaP for blossom blight disease management in ornamentals if applied at concentrations low enough to avoid phytotoxicity.


Assuntos
Fungicidas Industriais , Humanos , Fungicidas Industriais/farmacologia , Botrytis , Flores , Doenças das Plantas/prevenção & controle , Doenças das Plantas/microbiologia , Farmacorresistência Fúngica
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