Dual-Wave Acoustofluidic Centrifuge for Ultrafast Concentration of Nanoparticles and Extracellular Vesicles.

Extracellular vesicles (EVs) are secreted nanostructures that play various roles in critical cancer processes. They operate as an intercellular communication system, transferring complex sets of biomolecules from cell to cell. The concentration of EVs is difficult to decipher, and there is an unmet technological need for improved (faster, simpler, and gentler) approaches to isolate EVs from complex matrices. Herein, an acoustofluidic concentration of extracellular vesicles (ACEV) is presented, based on a thin-film printed circuit board with interdigital electrodes mounted on a piezoelectric substrate. An angle of 120° is identified between the electrodes and the reference flat of the piezoelectric substrate for simultaneous generation of Rayleigh and shear horizontal waves. The dual waves create a complex acoustic field in a droplet, resulting in effective concentration of nanoparticles and EVs. The ACEV is able to concentrate 20 nm nanospheres within 105 s and four EV dilutions derived from the human prostate cancer (Du145) cell line in approximately 30 s. Cryo-electron microscopy confirmed the preservation of EV integrity. The ACEV device holds great potential to revolutionize investigations of EVs. Its faster, simpler, and gentler approach to EV isolation and concentration can save time and effort in phenotypic and functional studies of EVs.

Impact Dynamics of Non-Newtonian Droplets on Superhydrophobic Surfaces.

Droplet impact behavior on a solid surface is critical for many industrial applications such as spray coating, food production, printing, and agriculture. For all of these applications, a common challenge is to modify and control the impact regime and contact time of the droplets. This challenge becomes more critical for non-Newtonian liquids with complex rheology. In this research, we explored the impact dynamics of non-Newtonian liquids (by adding different concentrations of Xanthan into water) on superhydrophobic surfaces. Our experimental results show that by increasing the Xanthan concentration in water, the shapes of the bouncing droplet are dramatically altered, e.g., its shape at the separation moment is changed from a conventional vertical jetting into a "mushroom"-like one. As a result, the contact time of the non-Newtonian droplet could be reduced by up to ∼50%. We compare the impact scenarios of Xanthan liquids with those of glycerol solutions having a similar apparent viscosity, and results show that the differences in the elongation viscosity induce different impact dynamics of the droplets. Finally, we show that by increasing the Weber number for all of the liquids, the contact time is reduced, and the maximum spreading radius is increased.

Untangling the mechanics of entanglements in slide-ring gels towards both super-deformability and toughness.

Entanglement plays a critical role in determining the dynamic properties of polymer systems, e.g., resulting in slip links and pulley effects for achieving large deformation and high strength. Although it has been studied for decades, the mechanics of entanglements for stiffness-toughness conflict is not well understood. In this study, topological knot theory incorporating an extended tube model is proposed to understand the entanglements in a slide-ring (SR) gel, which slips over a long distance to achieve large deformation and high toughness via the pulley effect. Based on topological knot theory, the sliding behavior and pulley effect of entanglements among molecular chains and cross-linked rings are thoroughly investigated. Based on rubber elasticity theory, a free-energy function is formulated to describe mechanical toughening and slipping of topological knots, while the SR gel retains the same binding energy. Finally, the effectiveness of the proposed model is verified using both finite element analysis and experimental results reported in the literature.

Simulation of a Temperature-Compensated Voltage Sensor Based on Photonic Crystal Fiber Infiltrated with Liquid Crystal and Ethanol.

A simulated design for a temperature-compensated voltage sensor based on photonic crystal fiber (PCF) infiltrated with liquid crystal and ethanol is presented in this paper. The holes distributed across the transverse section of the PCF provide two channels for mode coupling between the liquid crystal or ethanol and the fiber core. The couplings are both calculated accurately and explored theoretically using the finite element method (FEM). The influence of voltage on the alignment of the liquid crystal molecules and confinement loss of the fiber mode are studied. Liquid crystal molecules rotate which changes its properties as the voltage changes. As the characteristics of the liquid crystal will be affected by temperature, therefore, we further fill using ethanol, which is merely sensitive to temperature, into one hole of the PCF to realize temperature compensation. The simulated results show that the sensitivity is up to 1.29977 nm/V with the temperature of 25 °C when the voltage ranges from 365 to 565 V. The standard deviation of the wavelength difference is less than 2 nm within the temperature adjustment from 25 to 50 °C for temperature compensation. The impacts of the construction parameters of the PCF on sensing performances of this voltage sensor are also analyzed in this paper.

Anchoring-mediated topology signature of self-assembled elastomers undergoing mechanochromic coupling/decoupling.

Soft elastomers with their ability to integrate strain-adaptive stiffening and coloration have recently received significant research attention for application in artificial muscle and active camouflage. However, there is a lack of theoretical understanding of their complex molecular dynamics and mechanochromic coupling/decoupling. In this study, a topological dynamics model is proposed to understand the anchoring-mediated topology signature of self-assembled elastomers. Based on the constrained molecular junction model, a free-energy function is firstly formulated to describe the working principles of strain-adaptive stiffening and coloration in the self-assembled elastomer. A coupled ternary "rock-paper-scissors" model is proposed to describe the topological dynamics of self-assembly, mechanochromic coupling and mechanoresponsive stiffening of the self-assembled elastomers, in which there are three fractal geometry components in the topology network. Finally, the proposed models are verified using the experimental results reported in the literature. This study provides a fundamental approach to understand the working mechanism and topological dynamics in the self-assembled elastomers, with molecularly encoded stiffening and coloration.

A stimuli-responsive gel impregnated surface with switchable lipophilic/oleophobic properties.

In this paper, we developed a novel morphing surface technique consisting of a 3D printed miniature groove structure and injected stimuli-responsive hydrogel pattern, which is capable of switching between lipophilicity and oleophobicity under certain stimuli. Under swelling, the geometrical change of the hydrogel will buckle the surface due to the structural confinement and create a continuous transition of surface topology. Thus, it will yield a change in the surface wetting property from oleophilic to super-oleophobic with a contact angle of oil of 85° to 165°. We quantitatively investigate this structure-property relationship using finite element analysis and analytical modeling, and the simulation results and the modeling are in good agreement with the experimental ones. This morphing surface also holds potential to be developed into an autonomous system for future sub-sea/off-shore engineering applications to separate oil and water.

Integrating Radio-Over-Fiber Communication System and BOTDR Sensor System.

In this paper, we propose and experimentally demonstrate for the first time, the integration of a radio-over-fiber (RoF) communication system and a Brillouin optical time-domain reflectometry (BOTDR) distributed sensor system using a single optical fiber link. In this proof-of-concept integrated system, the communication system is composed of three modulation formats of quadrature phase-shift keying (QPSK), 16-quadrature amplitude modulation (16-QAM) and 64-QAM, which are modulated onto an orthogonal frequency division multiplexing (OFDM) signal. Whereas, the BOTDR sensor system is used for strain and/or temperature monitoring over the fiber distance with a spatial resolution of 5 m using a 25 km single-mode silica fiber. The error vector magnitude (EVM) is analyzed in three modulation formats in the presence of various BOTDR input pump powers. Using QPSK modulation, optimized 18 dBm sensing and 10 dBm data power, the measured EVM values with and without bandpass filter are 3.5% and 14.5%, respectively. The proposed system demonstrates a low temperature measurement error (±0.49 °C at the end of 25 km) and acceptable EVM values, which were within the 3GPP requirements. The proposed integrated system can be effectively applied for practical applications, which significantly reduces the fiber infrastructure cost by effective usage of a single optical fiber link.

Room-Temperature Ammonia Sensor Based on ZnO Nanorods Deposited on ST-Cut Quartz Surface Acoustic Wave Devices.

Using a seed layer-free hydrothermal method, ZnO nanorods (NRs) were deposited on ST-cut quartz surface acoustic wave (SAW) devices for ammonia sensing at room temperature. For a comparison, a ZnO film layer with a thickness of 30 nm was also coated onto an ST-cut quartz SAW device using a sol-gel and spin-coating technique. The ammonia sensing results showed that the sensitivity, repeatability and stability of the ZnO NR-coated SAW device were superior to those of the ZnO film-coated SAW device due to the large surface-to-volume ratio of the ZnO NRs.

High Sensitivity Refractometer Based on Reflective Smf-Small Diameter No Core Fiber Structure.

A high sensitivity refractive index sensor based on a single mode-small diameter no core fiber structure is proposed. In this structure, a small diameter no core fiber (SDNCF) used as a sensor probe, was fusion spliced to the end face of a traditional single mode fiber (SMF) and the end face of the SDNCF was coated with a thin film of gold to provide reflective light. The influence of SDNCF diameter and length on the refractive index sensitivity of the sensor has been investigated by both simulations and experiments, where results show that the diameter of SDNCF has significant influence. However, SDNCF length has limited influence on the sensitivity. Experimental results show that a sensitivity of 327 nm/RIU (refractive index unit) has been achieved for refractive indices ranging from 1.33 to 1.38, which agrees well with the simulated results with a sensitivity of 349.5 nm/RIU at refractive indices ranging from 1.33 to 1.38.

Synchronization of Coupled Oscillators on a Two-Dimensional Plane.

The effect of the transfer rate of signal molecules on coupled chemical oscillators arranged on a two-dimensional plane was systematically investigated in this paper. A microreactor equipped with a surface acoustic wave (SAW) mixer was applied to adjust the transfer rate of the signal molecules in the microreactor. The SAW mixer with adjustable input powers provided a simple means to generate different mixing rates in the microreactor. A robust synchronization of the oscillators was found at an input radio frequency power of 20 dBm, with which the chemical waves were initiated at a fixed site of the oscillator system. With increasing input power, the frequency of the chemical waves was increased, which agreed well with the prediction given by the time-delayed phase oscillator model. Results from the finite element simulation agreed well with the experimental results.

Micro-mechanics of nanostructured carbon/shape memory polymer hybrid thin film.

This paper investigates the mechanics of hybrid shape memory polymer polystrene (PS) based nanocomposites with skeletal structures of CNFs/MWCNTs formed inside. Experimental results showed an increase of glass transition temperature (Tg) with CNF/MWCNT concentrations instead of a decrease of Tg in nanocomposites filled by spherical particles, and an increase in mechanical properties on both macro- and µm-scales. Compared with CNFs, MWCNTs showed a better mechanical enhancement for PS nanocomposites due to their uniform distribution in the nanocomposites. In nanoindentation tests using the Berkovich tips, indentation size effects and pile-up effects appeared obviously for the nanocomposites, but not for pure PS. Experimental results revealed the enhancement mechanisms of CNFs/MWCNTs related to the secondary structures formed by nanofillers, including two aspects, i.e., filler-polymer interfacial connections and geometrical factors of nanofillers. The filler-polymer interfacial connections were strongly dependent on temperature, thus leading to the opposite changing trend of loss tangent with nanofiller concentrations, respectively, at low and high temperature. The geometrical factors of nanofillers were related to testing scales, further leading to the appearance of pile-up effects for nanocomposites in the nanoindentation tests, in which the size of indents was close to the size of the nanofiller skeleton.

In situ synchrotron X-ray diffraction analysis of deformation behaviour in Ti-Ni-based thin films.

Deformation mechanisms of as-deposited and post-annealed Ti50.2Ni49.6, Ti50.3Ni46.2Cu3.5 and Ti48.5Ni40.8Cu7.5 thin films were investigated using the in situ synchrotron X-ray diffraction technique. Results showed that initial crystalline phases determined the deformation mechanisms of all the films during tensile loading. For the films dominated by monoclinic martensites (B19'), tensile stress induced the detwinning of 〈011〉 type-II twins and resulted in the preferred orientations of (002)B19' parallel to the loading direction (∥ LD) and (020)B19' perpendicular to the LD (⊥ LD). For the films dominated by austenite (B2), the austenite directly transformed into martensitic variants (B19') with preferred orientations of (002)B19' ∥ LD and (020)B19' ⊥ LD. For the Ti50.3Ni46.2Cu3.5 and Ti48.1Ni40.8Cu7.5 films, martensitic transformation temperatures decreased apparently after post-annealing because of the large thermal stress generated in the films due to the large differences in thermal expansion coefficients between the film and substrate.

Capillary wave tweezer.

Precise control of microparticle movement is crucial in high throughput processing for various applications in scalable manufacturing, such as particle monolayer assembly and 3D bio-printing. Current techniques using acoustic, electrical and optical methods offer precise manipulation advantages, but their scalability is restricted due to issues such as, high input powers and complex fabrication and operation processes. In this work, we introduce the concept of capillary wave tweezers, where mm-scale capillary wave fields are dynamically manipulated to control the position of microparticles in a liquid volume. Capillary waves are generated in an open liquid volume using low frequency vibrations (in the range of 10-100 Hz) to trap particles underneath the nodes of the capillary waves. By shifting the displacement nodes of the waves, the trapped particles are precisely displaced. Using analytical and numerical models, we identify conditions under which a stable control over particle motion is achieved. By showcasing the ability to dynamically control the movement of microparticles, our concept offers a simple and high throughput method to manipulate particles in open systems.

High-sensitivity narrow­band T-shaped cantilever Fabry-perot acoustic sensor for photoacoustic spectroscopy.

Photoacoustic spectroscopy (PAS) has been rapidly developed and applied to different detection scenarios. The acoustic pressure detection is an important part in the PAS system. In this paper, an ultrahigh sensitivity Fabry-Perot acoustic sensor with a T-shaped cantilever was proposed. To achieve the best acoustic pressure effect, the dimension of the cantilever structure was designed and optimized by finite element analysis using COMSOL Multiphysics. Simulation results showed that the sensitivity of such T-shaped cantilever was 1.5 times higher than that based on a rectangular cantilever, and the resonance frequency of T-shaped cantilever were able to modulate from 800 Hz to 1500 Hz by adjusting the multi-parameter characteristics. Experimental sensing results showed that the resonance frequency of T-shaped Fabry-Perot acoustic sensor was 1080 Hz, yielding a high sensitivity of 1.428 µm/Pa, with a signal-to-noise ratio (SNR) of 84.8 dB and a detectable pressure limit of 1.9 µPa/Hz1/2@1 kHz. We successfully used such acoustic sensor to measure acetylene (C2H2) concentration in the PAS. The sensitivity of PAS for C2H2 gas was 3.22 pm/ppm with a concentration range of 50 ppm ∼100 ppm, and the minimum detection limit was 24.91ppb.

A nanonewton force sensor using a U-shape tapered microfiber interferometer.

Nanomechanical measurements, especially the detection of weak contact forces, play a vital role in many fields, such as material science, micromanipulation, and mechanobiology. However, it remains a challenging task to realize the measurement of ultraweak force levels as low as nanonewtons with a simple sensing configuration. In this work, an ultrasensitive all-fiber nanonewton force sensor structure based on a single-mode-tapered U-shape multimode-single-mode fiber probe is proposed and experimentally demonstrated with a limit of detection of ~5.4 nanonewtons. The use of the sensor is demonstrated by force measurement on a human hair sample to determine the spring constant of the hair. The results agree well with measurements using an atomic force microscope for the spring constant of the hair. Compared with other force sensors based on optical fiber in the literature, the proposed all-fiber force sensor provides a substantial advancement in the minimum detectable force possible, with the advantages of a simple configuration, ease of fabrication, and low cost.

Protective effects of ginseng total saponins against hepatic ischemia/reperfusion injury in experimental obstructive jaundice rats.

CONTEXT: Ginseng, the root of Panax ginseng C.A. Meyer (Araliaceae), is one of the most extensively used herbs for stroke and chronic debilitating conditions in East Asian countries. Ginsenosides (GS) are the main bioactive compounds for ginseng's efficacy, but the mechanisms have not been fully clarified. OBJECTIVE: To investigate hepatoprotective effects of GS against ischemia/reperfusion (IR) injury in the experimental obstructive jaundice rats. MATERIALS AND METHODS: GS was fed to cholestatic rats with IR injury daily for 6 d at a dose of 1.10 g/kg. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined by colorimetric method. Apoptosis was measured quantitatively by the terminal transferase UTP nick end-labeling method. Protein expression of Bax and Bcl-2 was detected by immunohistochemistry. RESULTS AND DISCUSSION: After intervention of GS to cholestatic rats with IR injury, the levels of activating blood flow were significantly improved, and the levels of serum ALT were decreased 1.7-times, AST decreased 1.3-times, but SOD activities were increased 1.1-times compared with those of the model rats. It could also reverse histopathological changes and inhibit IR-induced apoptosis of hepatic tissues via decrease of Bax/Bcl-2 ratio (from 2.87 ± 0.57 to 1.65 ± 0.29). Oral administration of GS in a dosage of 26.4 g/kg did not lead to toxic effects in rats. CONCLUSION: GS attenuated the IR injury in the presence of cholestasis and could be considered for the clinical treatment of cholestasis.

A phase transition model in dual-amorphous water undergoing liquid-liquid transition.

An in-depth understanding of liquid-liquid phase transition (LLPT) in condensed water will gain insight into anomalous behaviors of dual-amorphous condensed water. Despite numerous experimental, molecular simulation, and theoretical studies, it is yet to achieve a widely accepted consensus with convinced evidence in the condensed matter physics for two-state liquid-liquid transition of water. In this work, a theoretical model is proposed based on the Avrami equation, commonly used to describe first-order phase transitions, to elucidate complex homogeneous and inhomogeneous condensation from high-density liquid (HDL) water to low-density liquid (LDL) water for both pure and ionic dual-amorphous condensed water. This model unifies the coupling effects of temperature and electrolyte concentration based on the new theoretical framework. The Adam-Gibbs theory is then introduced to characterize the synergistic motion and relaxation behavior of condensed water. Variations in the configurational entropy under electrostatic forces are further explored, and an analytical 2D cloud chart is developed to visualize the synergistic effect of temperature and electrolyte concentration on the configurational entropy of ionic water. The constitutive relationships among viscosity, temperature, and electrolyte concentration are derived to analyze their synergistic effects under different condensation fractions of LDL and HDL. The Stokes-Einstein relation and free volume theory are further used to analyze diffusion coefficients and densities (or apparent density) during both pure and ionic LLPT. Finally, theoretical results obtained from these models are compared with experimental results reported in literature to validate the accuracy and applicability of the proposed models, which offer significant benefits and advancements in effectively predicting physical property changes of dual-amorphous condensed water.

The novel π-d conjugated TM2B3N3S6 (TM = Mo, Ti and W) monolayers as highly active single-atom catalysts for electrocatalytic synthesis of ammonia.

Recently, single-atom catalysts (SACs) are receiving significant attention in electrocatalysis fields due to their excellent specific activities and extremely high atomic utilization ratio. Effective loading of metal atoms and high stability of SACs increase the number of exposed active sites, thus significantly improving their catalytic efficiency. Herein, we proposed a series (29 in total) of two-dimensional (2D) conjugated structures of TM2B3N3S6 (TM means those 3d to 5d transition metals) and studied the performance as single-atom catalysts for nitrogen reduction reaction (NRR) using density functional theory (DFT). Results show that TM2B3N3S6 (TM = Mo, Ti and W) monolayers have superior performance for ammonia synthesis with low limiting potentials of -0.38, -0.53 and -0.68 V, respectively. Among them, the Mo2B3N3S6 monolayer shows the best catalytic performance of NRR. Meanwhile, the π conjugated B3N3S6 rings undergo coordinated electron transfer with the d orbitals of TM to exhibit good chargeability, and these TM2B3N3S6 monolayers activate isolated N2 according to the "acceptance-donation" mechanism. We have also verified the good stability (i.e., Ef < 0, and Udiss > 0) and high selectivity (Ud = -0.03, 0.01 and 0.10 V, respectively) of the above four types of monolayers for NRR over hydrogen evolution reaction (HER). The NRR activities have been clarified by multiple-level descriptors (ΔG*N2H, ICOHP, and Ɛd) in the terms of basic characteristics, electronic property, and energy. Moreover, the aqueous solution can promote the NRR process, leading to the reduction of ΔGPDS from 0.38 eV to 0.27 eV for the Mo2B3N3S6 monolayer. However, the TM2B3N3S6 (TM = Mo, Ti and W) also showed excellent stability in aqueous phase. This study proves that the π-d conjugated monolayers of TM2B3N3S6 (TM = Mo, Ti and W) as electrocatalysts show great potentials for the nitrogen reduction.

Metal-organic framework-derived trimetallic oxides with dual sensing functions for ethanol.

Metal-organic framework (MOF)-derived metal oxide semiconductors have recently received extensive attention in gas sensing applications due to their high porosity and three-dimensional architecture. Still, challenges remain for MOF-derived materials, including low-cost and facile synthetic methods, rational nanostructure design, and superior gas-sensing performances. Herein, a series of Fe-MIL-88B-derived trimetallic FeCoNi oxides (FCN-MOS) with a mesoporous structure were synthesized by a one-step hydrothermal reaction followed by calcination. The FCN-MOS system consists of three main phases: α-Fe2O3 (n-type), CoFe2O4, and NiFe2O4 (p-type), and the nanostructure and pore size can be controlled by altering the content of α-Fe2O3, CoFe2O4, and NiFe2O4. The sensors based on FCN-MOS exhibit a high response of 71.9, a good selectivity towards 100 ppm ethanol at 250 °C, and long-term stability up to 60 days. Additionally, the FCN-MOS-based sensors show a p-n transition gas sensing behavior with the alteration of the Fe/Co/Ni ratio.

Integrated label-free erbium-doped fiber laser biosensing system for detection of single cell Staphylococcus aureus.

A critical challenge to realize ultra-high sensitivity with optical fiber interferometers for label free biosensing is to achieve high quality factors (Q-factor) in liquid. In this work a high Q-factor of 105, which significantly improves the detection resolution is described based on a structure of single mode -core-only -single mode fiber (SCS) with its multimode (or Mach-Zehnder) interference effect as a filter that is integrated into an erbium-doped fiber laser (EDFL) system for excitation. In the case study, the section of core-only fiber is functionalized with porcine immunoglobulin G (IgG) antibodies, which could selectively bind to bacterial pathogen of Staphylococcus aureus (S. aureus). The developed microfiber-based biosensing platform called SCS-based EDFL biosensors can effectively detect concentrations of S. aureus from 10 to 105 CFU/mL, with a responsivity of 0.426 nm wavelength shift in the measured spectrum for S. aureus concentration of 10 CFU/mL. The limit of detection (LoD) is estimated as 7.3 CFU/mL based on the measurement of S. aureus with minimum concentration of 10 CFU/mL. In addition, when a lower concentration of 1 CFU/mL is applied to the biosensor, a wavelength shift of 0.12 nm is observed in 10% of samples (1/10), indicating actual LoD of 1 CFU/mL for the proposed biosensor. Attributed to its good sensitivity, stability, reproducibility and specificity, the proposed EDFL based biosensing platform has great potentials for diagnostics.