Le morte du tumour: histological features of tumor destruction in chemo-resistant cancers following intravenous infusions of pathotropic nanoparticles bearing therapeutic genes.

The pathotropic targeting of therapeutic nanoparticles to cancerous lesions is an innovative concept that has recently been reduced to practice in clinical trials for the treatment of metastatic cancer. Previously, we reported that intravenous infusions of Rexin-G, a pathotropic nanoparticle (or vector) bearing a cyto-ablative construct, induced tumor regression, reduced tumor burden, and improved survival, while enhancing the overall quality-of-life of patients with otherwise intractable chemotherapy-resistant cancers. In this report, we describe the major histopathological and radiologic features that are characteristic of solid tumors under the destructive influences of Rexin-G administered as a single therapeutic agent. To further promote tumor eradication and enhance cancer survival, we explored the potential of an auxiliary gene transfer strategy, specifically intended to induce a localized cancer auto-immunization in addition to assisting in acute tumor destruction. This immunization strategy uses Rexin-G in combination with Reximmune-C, a tumor targeted expression vector bearing a granulocyte macrophage-colony stimulating factor (GM-CSF) gene. Intravenous infusions of Rexin-G were given first to induce apoptosis and necrosis in the metastatic tumor nodules, thus exposing tumor neo-antigens, followed by Reximmune-C infusions, intended to recruit immune cells discretely into the same compartments (or lesions). The intent of this two-step approach is to bring a complement of cells involved in humoral and cell-mediated immunity in close proximity to the immunizing tumor antigens in a concerted effort to assist in tumor eradication and to promote a cancer vaccination in situ. Herein, we also describe the distinctive histopathologic and immunocytochemical features of tumors in terminal cancer patients who received Rexin-G infusions in combination with Reximmune-C. In addition to documenting the first histological indications of clinical efficacy achieved by this novel personalized approach to cancer vaccination, we discuss new methods and strategies for advancing its therapeutic utility. Taken together with the clinical data, these histological studies serve as valuable landmarks for medical oncology, and as definitive benchmarks for the emerging field of cancer gene therapy.

Comparison of three Dade-Behring Dimension AR machines in a clinical chemistry laboratory.

Three Dade-Behring Dimension AR Analyzers are presently in use in the Philippine General Hospital Department of Laboratories. One machine was acquired 2 years ago and two new ones were added recently. In order to determine whether the three machines would produce equivalent results 16 samples were selected for patients with hepatic and/or renal disorder. All 16 samples were tested for 16 different analytes. The results were subjected to tests for coefficient correlation, simple linear regression using slope and intercept and t-test for comparison. We concluded that for two assays (globulin and phosphorus), the values were sufficiently similar. For seven other assays (for total protein, albumin, sodium, potassium, chloride, calcium, and magnesium), the results were statistically significantly different, and for the remaining eight assays (total hiliruhin, direct bilirubin, AST, ALT, ALP, glucose, BUN, and creatinine), the values obtained were not ideal for drawing up conclusions about comparability. For the latter group of assays we plan to pool patient specimens of appropriate numbers and levels and repeat testing at some specified later date.

Current status of laboratory testing for HIV in the Philippines

Prevalence of HIV infection in the Philippines remains low. This may be partly due to reliance on passive reporting for surveillance. The algorithm for laboratory testing for HIV infection has become more stringent in the sense that the screening assays are repeated in the confirmatory centers, before Western Blot is performed. This has been due to the high rate of false positives before 2005. Nucleic Acid Amplification testing (NAAT) has been performed routinely for blood banking purposes in other countries. In a few pilot studies, it has proven useful in identifying those cases in the early stage of the infection, which are missed on testing by antibody-based assays. The assay may prove useful in knowing whether false negatives happen with the current testing algorithm in the Philippines. Coupled with the detuned assay, identification of new cases may be critical for prevention of transmission, surveillance of cases, and early medical management if needed.

Comparison of three Dade-Behring Dimension AR machines in a clinical chemistry laboratory.

Three Dade-Behring Dimension AR Analyzers are presently in use in the Philippine General Hospital Department of Laboratories. One machine was acquired 2 years ago and two new ones were added recently. In order to determine whether the three machines would produce equivalent results 16 samples were selected for patients with hepatic and/or renal disorder. All 16 samples were tested for 16 different analytes. The results were subjected to tests for coefficient correlation, simple linear regression using slope and intercept and t-test for comparison. We concluded that for two assays (globulin and phosphorus), the values were sufficiently similar. For seven other assays (for total protein, albumin, sodium, potassium, chloride, calcium, and magnesium), the results were statistically significantly different, and for the remaining eight assays (total hiliruhin, direct bilirubin, AST, ALT, ALP, glucose, BUN, and creatinine), the values obtained were not ideal for drawing up conclusions about comparability. For the latter group of assays we plan to pool patient specimens of appropriate numbers and levels and repeat testing at some specified later date.