Telomerase activity of basal cell carcinoma in patients living in North Queensland, Australia.

Basal cell carcinoma (BCC) is the most common skin cancer in North Queensland, Australia. The role of telomerase in BCC has not been investigated in this region. The objective of the study was to investigate the expression levels of human telomerase reverse transcriptase (hTERT) and telomerase activity in nodular BCCs and superficial BCCs from patients living in North Queensland. Matched tumor and adjacent nontumorous mucosa samples from 12 BCCs (7 nodular and 5 superficial) among 11 male patients were collected. In these samples, RNA was extracted and then transcribed to complementary DNA. Real-time polymerase chain reaction (PCR) was used to quantify the expression levels of hTERT messenger RNA (mRNA). In addition, telomeric repeat amplification protocol assay, a PCR-based method, was performed to detect telomerase activity in tissue extracts from these samples. The findings were correlated with the clinicopathologic features of patients with these tumors. All BCC samples expressed telomerase hTERT mRNA, and 75% (9/12) of the BCC samples showed telomerase activity. The mean level of expression among BCC samples was higher than that among matched nontumor samples (mean, 0.468 versus 0.140; P < .0001). Nodular BCC samples showed higher expression levels of hTERT mRNA as compared with superficial BCC samples (mean, 0.584 versus 0.305; P < .05). There was no significant association between expression of hTERT mRNA and tumor size. In conclusion, this study is the first on telomerase expression in BCC in Queensland as well as the first to analyze telomerase in BCC by quantitative PCR. The results indicate that telomerase alterations have an important role in the pathogenesis of BCCs. A unique finding is that the telomerase expression level in nodular BCCs is different from that in superficial BCCs.

Clinicopathological significance of synchronous carcinoma in colorectal cancer.

BACKGROUND: Synchronous colorectal carcinoma has seldom been studied in large series. The study was designed to examine the significance of colorectal synchronous carcinoma in a large cohort of patients. METHODS: The clinicopathological features of 102 patients with synchronous colorectal carcinoma were compared with 1,793 patients with solitary colorectal carcinoma. RESULTS: The prevalence of synchronous colorectal carcinoma was 3.6%. In these patients, 4% had FAP, 6% had hyperplastic polyposis, and 2% had ulcerative colitis. The index carcinoma was more likely to have higher histological grade and T stage than other carcinoma(s) in the same patient. When compared with solitary colorectal carcinoma, synchronous colorectal carcinoma was more often noted in males with coexisting FAP and in proximal location. The 5-year survival rate of patients with synchronous colorectal carcinoma was 53% and was similar to those with solitary colorectal carcinoma. CONCLUSIONS: We examined the clinicopathological features of patients with synchronous colorectal carcinomas in a large cohort of patients. Attention to these features was important for better management of this group of cancer.