RESUMO
SummaryPrevious studies have established a model of atresia in preovulatory follicles after stimulation of immature rats with equine chorionic gonadotropin (eCG). This gonadotropin recruits a follicular pool and the deprivation of preovulatory luteinizing hormone (LH) surge induces the atresia in preovulatory follicles. The present study investigated the occurrence of ovulation and provided some morphological features of granulosa cell (GC) apoptosis of atretic follicles at 0, 48, 72 and 120 h after eCG stimulation. Histological sections of ovaries from untreated animals (0 h) showed primordial, primary, secondary and early antral follicles. After 48 h ovaries showed large antral follicles. Preovulatory follicles were observed at 72 h, and two out of five rats displayed cumulus-oocyte complexes (COCs) in the oviducts. All animals exhibited corpora lutea after 120 h. We observed increased estradiol (E2) levels 48 h after eCG treatment that might trigger an endogenous preovulatory gonadotropin surge. Higher progesterone (P4) level, which is the hallmark of a functional corpus luteum, was observed at 120 h. Atresia in secondary and antral follicles was observed by pyknotic granulosa cell nuclei in histology and positive immunolabelling for cleaved caspase 3. We also observed macrophages in secondary and antral follicles in atresia. Transmission electron microscopy revealed GCs with compacted chromatin against the nuclear envelope, nuclear fragmentation, cell shrinkage and fragmentation. No preovulatory follicles showed apoptosis of GCs. In conclusion, our results suggested the occurrence of an endogenous gonadotropin surge, promoting ovulation and preventing atresia of preovulatory follicles.
Assuntos
Apoptose , Atresia Folicular/fisiologia , Células da Granulosa/patologia , Folículo Ovariano/patologia , Ovulação , Animais , Células Cultivadas , Feminino , Células da Granulosa/metabolismo , Folículo Ovariano/metabolismo , Ratos , Ratos WistarRESUMO
Parkinson's disease is the second most common neurodegenerative disease, with an estimated prevalence of 41/100,000 individuals affected aged between 40 and 49 years old and 1,900/100,000 aged 80 and over. Based on the essentiality of ascertaining which wearable devices have clinical literary evidence and with the purpose of analyzing the information revealed by such technologies, we conducted this scientific article of integrative review. It is an integrative review, whose main objective is to carry out a summary of the state of the art of wearable devices used in patients with Parkinson's disease. After the review, we retrieved 8 papers. Of the selected articles, only 3 were not systematic reviews; one was a series of cases and two prospective longitudinal studies. These technologies have a very rich field of application; however, research is still necessary to make such evaluations reliable and crucial to the well-being of these patients.
Assuntos
Doença de Parkinson/fisiopatologia , Dispositivos Eletrônicos Vestíveis , Humanos , Monitorização Ambulatorial/instrumentação , Monitorização Fisiológica/instrumentaçãoRESUMO
SUMMARY Parkinson's disease is the second most common neurodegenerative disease, with an estimated prevalence of 41/100,000 individuals affected aged between 40 and 49 years old and 1,900/100,000 aged 80 and over. Based on the essentiality of ascertaining which wearable devices have clinical literary evidence and with the purpose of analyzing the information revealed by such technologies, we conducted this scientific article of integrative review. It is an integrative review, whose main objective is to carry out a summary of the state of the art of wearable devices used in patients with Parkinson's disease. After the review, we retrieved 8 papers. Of the selected articles, only 3 were not systematic reviews; one was a series of cases and two prospective longitudinal studies. These technologies have a very rich field of application; however, research is still necessary to make such evaluations reliable and crucial to the well-being of these patients.
RESUMO A doença de Parkinson figura como a segunda doença neurodegenerativa mais comum. Sua prevalência é estimada de 41 por 100.000 pessoas entre 40 e 49 anos a 1.900 por 100.000 pessoas com 80 anos ou mais. Baseando-se na essencialidade de averiguar os dispositivos vestíveis que possuem evidências clínicas literárias e com o objetivo de analisar as informações reveladas por tais tecnologias, temos a construção deste artigo científico de revisão integrativa. Trata-se de uma revisão integrativa que tem como principal objetivo realizar um sumário do estado da arte de dispositivos vestíveis utilizados em pacientes com doença de Parkinson. Após realizada a revisão, obtiveram-se oito artigos. Pode-se observar que dos artigos selecionados, apenas três não eram revisões sistemáticas, sendo um deles uma série de casos e outros dois, estudos longitudinais prospectivos. A utilização dessas tecnologias possui um campo muito rico para atuar, contudo ainda são necessárias pesquisas para que tais avaliações sejam fidedignas e cruciais para o bem-estar desses pacientes.
Assuntos
Humanos , Doença de Parkinson/fisiopatologia , Dispositivos Eletrônicos Vestíveis , Monitorização Ambulatorial/instrumentação , Monitorização Fisiológica/instrumentaçãoRESUMO
Este trabalho buscou descrever uma das ações extensiva e interdisciplinar da LASEM da Universidade Federal dos Vales do Jequitinhonha e Mucuri, os Mutirões de Saúde. A liga acadêmica, firmada no tripé ensino, pesquisa e extensão, pretende aproximar o estudante da prática da atenção à saúde, com foco na vivência, para auxiliar o entendimento de como os fatores socioeconômicos e culturais influenciam no processo saúde-doença. Além disso, tem por objetivo descrever sua proposta e dinâmica, incentivar sua realização como uma atividade de Atenção Primária à Saúde, visto a sua importância para a formação médica. Os mutirões de saúde foram mobilizações coletivas, planejadas pela liga e pelo gestor de saúde local, com intuito de promover uma aproximação com a comunidade, permitir a aplicação do conhecimento construído nas reuniões da liga e ofertar serviços de saúde à população local. A inserção precoce do aluno de medicina na prática médica permite uma aproximação entre a instituição de ensino e a comunidade, de forma que é construído um ambiente de crescimento mútuo, tanto para a educação do aluno, quanto para a saúde da população. Isso permite que o estudante se desenvolva como agente transformador do meio. A experiência com os mutirões de saúde mostrou que são atividades eficazes para o aperfeiçoamento do ensino, para o compartilhamento de saberes entre profissionais, estudantes e a comunidade, bem como para o fortalecimento da liga acadêmica de semiologia médica dentro da instituição universitária de ensino. (AU)
This work sought to describe one of the extensive and interdisciplinary actions of LASEM of the Federal University of the Jequitinhonha and Mucuri Valleys, the Health Task Force. The academic league, signed on the tripod teaching, research and extension, aims to bring the student closer to the practice of attention to health, with a focus on the experience, to help the understanding of how socioeconomic and cultural factors influence the health-disease process. In addition, it aims to describe its proposal and dynamics, to encourage its realization as a Primary Health Care activity, given its importance for medical training. The health efforts were collective mobilizations, planned by the league and the local health manager, in order to promote a rapprochement with the community, to allow the application of knowledge built in league meetings and to offer health services to the local population. The precocious insertion of the medical student in medical practice allows an approximation between the educational institution and the community, so that an environment of mutual growth is built, both for the students education and for the health of the population. This allows the student to develop as an agent that transforms the environment. The experience with health efforts showed that they are effective activities for the improvement of teaching, for the sharing of knowledge among professionals, students and the community, as well as for the strengthening of the academic league of medical semiology within the university teaching institution. (AU)