1.
Angew Chem Int Ed Engl
; 57(23): 6863-6867, 2018 06 04.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29689604
RESUMO
Described here is an efficient stereoselective synthesis of vibegron enabled by an enzymatic dynamic kinetic reduction that proceeds in a high-pH environment. To overcome enzyme performance limitations under these conditions, a ketoreductase was evolved by a computationally and structurally aided strategy to increase cofactor stability through tighter binding.