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1.
Leukemia ; 31(12): 2702-2708, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-28663577

RESUMO

Myelodysplastic syndromes (MDS) represent a heterogeneous group of hematological clonal disorders. Here, we have tested the bone marrow (BM) cells from 38 MDS patients covering all risk groups in two immunodeficient mouse models: NSG and NSG-S. Our data show comparable level of engraftment in both models. The level of engraftment was patient specific with no correlation to any specific MDS risk group. Furthermore, the co-injection of mesenchymal stromal cells (MSCs) did not improve the level of engraftment. Finally, we have developed an in vitro two-dimensional co-culture system as an alternative tool to in vivo. Using our in vitro system, we have been able to co-culture CD34+ cells from MDS patient BM on auto- and/or allogeneic MSCs over 4 weeks with a fold expansion of up to 600 times. More importantly, these expanded cells conserved their MDS clonal architecture as well as genomic integrity.


Assuntos
Células da Medula Óssea/patologia , Síndromes Mielodisplásicas/patologia , Animais , Biomarcadores , Transplante de Medula Óssea , Aberrações Cromossômicas , Modelos Animais de Doenças , Feminino , Expressão Gênica , Genes Reporter , Xenoenxertos , Humanos , Imunofenotipagem , Masculino , Células-Tronco Mesenquimais , Camundongos , Camundongos Knockout , Síndromes Mielodisplásicas/diagnóstico , Síndromes Mielodisplásicas/genética , Síndromes Mielodisplásicas/metabolismo
2.
Cah Anesthesiol ; 40(6): 403-5, 1992.
Artigo em Francês | MEDLINE | ID: mdl-1477754

RESUMO

Perioperative blood salvage during hip and knee prosthetic surgery have been evaluated in a series of 345 patients who underwent a total of 426 surgical procedures. In 85 per cent of the cases, no additional homologous transfusion was required. In the patients who benefited from autotransfusion the quantity of bank blood transfused was 10 times less than in patients who were not autotransfused.


Assuntos
Transfusão de Sangue Autóloga/instrumentação , Hemodiluição/métodos , Prótese de Quadril , Prótese do Joelho , Idoso , Perda Sanguínea Cirúrgica , Transfusão de Sangue Autóloga/métodos , Separação Celular , França/epidemiologia , Humanos , Pessoa de Meia-Idade , Reoperação , Estudos Retrospectivos
4.
Theor Appl Genet ; 88(1): 33-41, 1994 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24185879

RESUMO

Methods based on geostatistics were applied to quantitative traits of agricultural interest measured on a collection of 547 wild populations of perennial ryegrass in France. The mathematical background of these methods, which resembles spatial autocorrelation analysis, is briefly described. When a single variable is studied, the spatial structure analysis is similar to spatial autocorrelation analysis, and a spatial prediction method, called "kriging", gives a filtered map of the spatial pattern over all the sampled area. When complex interactions of agronomic traits with different evaluation sites define a multivariate structure for the spatial analysis, geostatistical methods allow the spatial variations to be broken down into two main spatial structures with ranges of 120 km and 300 km, respectively. The predicted maps that corresponded to each range were interpreted as a result of the isolation-by-distance model and as a consequence of selection by environmental factors. Practical collecting methodology for breeders may be derived from such spatial structures.

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