RESUMO
Late blight disease caused by the plant pathogenic oomycete pathogen Phytophthora infestans is one of the most limiting factors in potato production. P. infestans is able to overcome introgressed late blight resistance by adaptation of effector genes. AVR1 is an RXLR effector that triggers immune responses when recognized by the potato resistance protein R1. P. infestans isolates avirulent on R1 plants were found to have AVR1 variants that are recognized by R1. Virulent isolates though, lack AVR1 but do contain a close homologue of AVR1, named A-L, of which all variants escape recognition by R1. Co-expression of AVR1 and R1 in Nicotiana benthamiana results in a hypersensitive response (HR). In contrast, HR is not activated when A-L is co-expressed with R1. AVR1 and A-L are highly similar in structure. They share two W motifs and one Y motif in the C-terminal part but differ in the T-region, a 38 amino acid extension at the carboxyl-terminal tail of AVR1 lacking in A-L. To pinpoint what determines R1-mediated recognition of AVR1 we tested elicitor activity of AVR1 and A-L chimeric and deletion constructs by co-expression with R1. The T-region is important as it enables R1-mediated recognition of A-L, not only when fused to A-L but also via trans-complementation. Yet, AVR1 lacking the T-region is still active as an elicitor of HR, but this activity is lost when certain motifs are swapped with A-L. These data show that A-L circumvents R1 recognition not only because it lacks the T-region, but also because of differences in the conserved C-terminal effector motifs.
RESUMO
G-protein-coupled receptors (GPCRs) are key cellular components that mediate extracellular signals into intracellular responses. Genome mining revealed that Phytophthora spp. have over 60 GPCR genes among which a prominent class of 12 encoding novel proteins with an N-terminal GPCR domain fused to a C-terminal phosphatidylinositol phosphate kinase (PIPK) domain. This study focuses on two GPCR-PIPKs (GKs) in Phytophthora sojae. PsGK4 and PsGK5 are differentially expressed during the life cycle with the highest expression in cysts and during cyst germination, and at late infection stages. In P. sojae transformants that constitutively express RFP-tagged PsGK4 and PsGK5, the fusion proteins in hyphae reside in small, rapidly moving vesicular-like structures. Functional analysis using gene silencing showed that PsGK4-silenced transformants displayed higher levels of encystment and a reduced cyst germination rate when compared with the recipient strain. Moreover, GK4 deficiency (or reduction) resulted in severe defects in zoospore chemotaxis towards isoflavones and soybean roots. In contrast, PsGK5-silenced transformants exhibited no obvious defects in asexual development but oospore production was severely impaired. Both, PsGK4- and PsGK5-silenced transformants showed reduced pathogenicity. These results point to involvement of GKs in zoospore behaviour, chemotaxis and oospore development, and suggest that PsGK4 and PsGK5 each head independent signalling pathways.
Assuntos
Quimiotaxia , Regulação da Expressão Gênica no Desenvolvimento , Fosfatos de Fosfatidilinositol/metabolismo , Fosfotransferases/metabolismo , Phytophthora/crescimento & desenvolvimento , Phytophthora/fisiologia , Receptores Acoplados a Proteínas G/química , Esporos/fisiologia , Proteínas de Algas/genética , Proteínas de Algas/metabolismo , Isoflavonas , Fosfotransferases/química , Fosfotransferases/genética , Phytophthora/genética , Phytophthora/metabolismo , Doenças das Plantas/microbiologia , Raízes de Plantas/microbiologia , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo , Transdução de Sinais , Glycine max/microbiologiaRESUMO
Phenotypic and genotypic characteristics of 48 Phytophthora infestans isolates, collected in five provinces in Northern China between 1997 and 2003, were determined and compared with reference isolates. Characterisation included mating type, virulence, mitochondrial DNA (mtDNA) haplotype and DNA fingerprinting patterns based on simple sequence repeats (SSR) and amplified fragment length polymorphisms (AFLP). All isolates had the A1 mating type, mtDNA haplotype IIa and an identical SSR genotype (designated as SG-01-01) that differed from SSR genotypes found in the reference isolates, including those representing the 'old' US-1 lineage that dominated the P. infestans population worldwide prior to 1980. In contrast, the virulence spectra were highly variable and virulence to all resistance genes present in the standard differential set (R1 to R11) was found. AFLP analysis revealed some diversity; eight different AFLP genotypes were found that could be grouped into two major clusters. This study shows that there is very little genotypic diversity in the P. infestans population in Northern China. The occurrence of many different races within this rather uniform population is discussed in the framework of recent insights into the molecular determinants of avirulence in potato-P. infestans'gene-for-gene' interactions.
Assuntos
Variação Genética , Phytophthora infestans/genética , Phytophthora infestans/patogenicidade , Doenças das Plantas/microbiologia , Solanum tuberosum/microbiologia , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos , China , DNA Mitocondrial/genética , Genótipo , Haplótipos , Repetições Minissatélites , Fenótipo , Dinâmica Populacional , Virulência/genéticaRESUMO
Phytophthora infestans, the agent of potato and tomato late blight disease, produces a 10-kD extracellular protein, INF1 elicitin. INF1 induces a hypersensitive response in a restricted number of plants, particularly those of the genus Nicotiana. In virulence assays with different P. infestans isolates, five Nicotiana species displayed resistance responses. In all of the interactions, after inoculation with P. infestans zoospores, penetration of an epidermal cell was observed, followed by localized necrosis typical of a hypersensitive response. To determine whether INF1 functions as an avirulence factor in these interactions, we adopted a gene-silencing strategy to inhibit INF1 production. Several transformants deficient in inf1 mRNA and INF1 protein were obtained. These strains remained pathogenic on host plants. However, in contrast to the wild-type and control transformant strains, INF1-deficient strains induced disease lesions when inoculated on N. benthamiana. These results demonstrate that the elicitin INF1 functions as an avirulence factor in the interaction between N. benthamiana and P. infestans.
RESUMO
The cytogenetics of Rauscher murine leukemia virus-induced erythroid, myeloid, and lymphatic leukemias were studied in BALB/c and DBA/2 mice. In primary virus-induced leukemias, no chromosome abnormalities were found. However, in tumors derived from transplanted leukemia tissue and in cell lines obtained from these tumors, euploidy and mostly aneuploidy were observed that varied according to the type of tumor cells and the number of passages. Eleven erythroleukemia cell lines showed aneuploidy from the first in vivo transplant stage on. Nonrandom abnormalities were found: trisomy 15 in 9 of 11 lines, followed by trisomy 3 in 8 of 11 lines and monosomy 6 in 5 of 11 lines. Subsequent evolution of the karyotypes was frequent (10 of 11 lines) and rapid. In vivo cell lines established from these tumors showed many structural rearrangements. Three myeloid lines revealed a stable karyotype with no or only minor changes: trisomy 15 in 1 line and normal diploid in 2 lines. One lymphatic leukemia cell line established in vitro presented with a very stable karyotype: trisomies 14 and 15. Another in vivo transplantable line showed trisomies 11, 9, and 17. These results suggest that trisomy of chromosome 15 plays a significant role in tumors derived from different types of mouse leukemias.
Assuntos
Aberrações Cromossômicas , Leucemia Experimental/genética , Trissomia , Infecções Tumorais por Vírus/genética , Aneuploidia , Animais , Linhagem Celular , Feminino , Cariotipagem , Leucemia Eritroblástica Aguda/genética , Masculino , Camundongos , Camundongos Endogâmicos , Transplante de Neoplasias , Vírus RauscherRESUMO
In this study we investigated the genetic control of avirulence in the diploid oomycete pathogen Phytophthora infestans, the causal agent of late blight on potato. The dominant avirulence (Avr) genes matched six race-specific resistance genes introgressed in potato from a wild Solanum species. AFLP markers linked to Avr genes were selected by bulked segregant analysis and used to construct two high-density linkage maps, one containing Avr4 (located on linkage group A2-a) and the other containing a cluster of three tightly linked genes, Avr3, Avr10, and Avr11 (located on linkage group VIII). Bulked segregant analysis also resulted in a marker linked to Avr1 and this allowed positioning of Avr1 on linkage group IV. No bulked segregant analysis was performed for Avr2, but linkage to a set of random markers placed Avr2 on linkage group VI. Of the six Avr genes, five were located on the most distal part of the linkage group, possibly close to the telomere. The high-density mapping was initiated to facilitate future positional cloning of P. infestans Avr genes.
Assuntos
Phytophthora/genética , Polimorfismo Genético/genética , Virulência/genética , Mapeamento Cromossômico , Clonagem Molecular , Impressões Digitais de DNA , Genes Dominantes , Ligação Genética , Marcadores Genéticos , Genótipo , Escore Lod , Modelos Genéticos , FenótipoRESUMO
Elicitins are a family of structurally related proteins that induce hypersensitive response in specific plant species. Two Phytophthora infestans cDNAs, inf2A and inf2B, potentially encoding novel elicitin-like proteins, were isolated from a cDNA library made from infected potato tissue. Multiple sequence alignments and phylogenetic analyses of 19 elicitins and elicitin-like proteins from nine Phytophthora spp. and from Pythium vexans suggest that there are at least five distinct classes within the elicitin family.
Assuntos
Proteínas de Algas , Proteínas Fúngicas/genética , Genes Fúngicos , Micotoxinas/genética , Phytophthora/genética , Sequência de Aminoácidos , Proteínas Fúngicas/classificação , Dados de Sequência Molecular , Doenças das Plantas/genética , Proteínas , Pythium/genética , Homologia de Sequência de Aminoácidos , Especificidade da EspécieRESUMO
Recent phylogenetic analyses of the nucleotide binding sites (NBS)-leucine-rich repeats (LRR) class of plant disease resistance (R) genes suggest that these genes are ancient and coexist next to susceptibility alleles at resistance loci. Another class of R genes encodes serine-threonine protein kinases related to Pto that were originally identified from wild relatives of tomato. In this study, we exploit the highly diverse genus Solanum to identify Pto-like sequences and test various evolutionary scenarios for Pto-like genes. Polymerase chain reaction amplifications with the use of primers that were developed on the basis of conserved and variable regions of Pto revealed an extensive Pto gene family and yielded 32 intact Pto-like sequences from six Solanum species. Furthermore, Pto-like transcripts were detected in the leaf tissue of all tested plants. The kinase consensus and autophosphorylation sites were highly conserved, in contrast to the kinase activation domain, which is involved in ligand recognition in Pto. Phylogenetic analyses distinguished nine classes of Pto-like genes and revealed that orthologs were more similar than paralogs, suggesting that the Pto gene family evolved through a series of ancient gene duplication events prior to speciation in Solanum. Thus, like the NBS-LRR class, the kinase class of R genes is highly diverse and ancient.
Assuntos
Evolução Biológica , Genes de Plantas , Família Multigênica , Proteínas de Plantas , Proteínas Serina-Treonina Quinases/genética , Solanaceae/genética , Sequência de Aminoácidos , Domínio Catalítico/genética , Sequência Conservada , Primers do DNA , Modelos Genéticos , Dados de Sequência Molecular , Filogenia , Doenças das Plantas/genética , Folhas de Planta/genética , Solanaceae/classificação , Solanaceae/enzimologia , Especificidade da EspécieRESUMO
In order to isolate in planta-induced genes encoding putative pathogenicity factors of the late blight fungus Phytophthora infestans, a genomic library was differentially screened. For the differential hybridization, labeled first-strand cDNA synthesized on mRNA isolated from P. infestans-infected potato leaves and on mRNA isolated from the fungus grown in vitro were used as probes. This screening resulted in the isolation of the P. infestans calmodulin gene. The gene, designated calA, contains an open reading frame of 447 base pairs without introns and is unique in the P. infestans genome. The predicted amino acid sequence is 89.9-94.6% identical to calmodulins from higher eukaryotes, whereas the identity to calmodulins of higher fungi is significantly less (60.8-85.1%). Expression studies revealed that the P. infestans calA gene is constitutively expressed in in vitro grown mycelium. However, during pathogenesis on potato the level of P. infestans calmodulin mRNA is increased approximately fivefold.
Assuntos
Calmodulina/genética , Genes Bacterianos/genética , Phytophthora/genética , Doenças das Plantas/etiologia , Solanum tuberosum/microbiologia , Sequência de Aminoácidos , Sequência de Bases , Calmodulina/biossíntese , Expressão Gênica , Dados de Sequência Molecular , Phytophthora/patogenicidade , Doenças das Plantas/microbiologia , RNA Mensageiro/genética , Sequências Reguladoras de Ácido Nucleico/genética , Mapeamento por Restrição , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
In Phytophthora infestans, a cluster of three dominant avirulence genes is located on the distal part of linkage group VIII. In a mapping population from a cross between two Dutch field isolates, probe M5.1, derived from an amplified fragment length polymorphism (AFLP) marker linked to the Avr3-Avr10-Avr11 cluster, hybridized only to DNA from the parent and F1 progeny that is avirulent on potato lines carrying the R3, R10, and R11 resistance gene. In the virulent parent and the virulent progeny, no M5.1 homologue was detected, demonstrating a deletion on that part of linkage group VIII. P. infestans is diploid, so the avirulent strains must be hemizygous for the region concerned. A similar situation was found in another mapping population from two Mexican strains. The deletion was also found to occur in many field isolates. In a large set of unique isolates collected in The Netherlands from 1980 to 1991, 37% had no M5.1 homologue and the deletion correlated strongly with gain of virulence on potato lines carrying R3, R10, and R11. Also, in some old isolates that belong to a single clonal lineage (US-1) and are thus highly homogenous, deletions at the M5.1 locus were detected, indicating that this region is unstable.
Assuntos
Deleção Cromossômica , Phytophthora/genética , Phytophthora/patogenicidade , Solanum tuberosum/genética , Solanum tuberosum/microbiologia , Southern Blotting , Cruzamentos Genéticos , Ligação Genética , Marcadores Genéticos , Genótipo , Família Multigênica , Polimorfismo Genético , Solanum tuberosum/classificação , Virulência/genéticaRESUMO
Most species of the genus Phytophthora produce 10-kDa extracellular protein elicitors, collectively termed elicitins. Elicitins induce hypersensitive response in a restricted number of plants, particularly in the genus Nicotiana within the Solanaceae family. A cDNA encoding INF1, the major secreted elicitin of Phytophthora infestans, a pathogen of solanaceous plants, was isolated and characterized. The expression of the corresponding inf1 gene during the disease cycle of P. infestans was analyzed. inf1 was shown to be expressed in mycelium grown in various culture media, whereas it was not expressed in sporangiospores, zoospores, cysts, and germinating cysts. In planta, during infection of potato, particularly during the biotrophic stage, expression of inf1 was down-regulated compared to in vitro. The highest levels of expression of inf1 were observed in in vitro grown mycelium and in late stages of infection when profuse sporulation and leaf necrosis occur. The potential role of INF1 as an elicitor in interactions between P. infestans and Solanum species was investigated. Nineteen lines, representing nine solanaceous species with various levels of resistance to P. infestans, were tested for response to an Escherichia coli expressed INF1. Within the genus Solanum, resistance to P. infestans did not appear to be mediated by a defense response elicited by INF1. However, INF1 recognition could be a component of nonhost resistance of tobacco to P. infestans.
Assuntos
Proteínas Fúngicas/biossíntese , Regulação Fúngica da Expressão Gênica , Phytophthora/genética , Solanum tuberosum/microbiologia , Proteínas de Algas , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Escherichia coli , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Regulação da Expressão Gênica no Desenvolvimento , Genes Fúngicos , Dados de Sequência Molecular , Phytophthora/patogenicidade , Folhas de Planta , Proteínas , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/químicaRESUMO
From a set of Phytophthora infestans cDNA clones randomly selected from a potato-P. infestans interaction cDNA library, three out of 22 appeared to correspond to a gene encoding translation elongation factor 1alpha. The gene, called tef1, is a single copy gene in P. infestans. During the life cycle of P. infestans, tef1 is expressed in all developmental stages. Alignment and phylogeny analysis based on EF-1alpha proteins from several taxonomic groups, including fungi, slime molds, algae, higher plants and archeabacteria, support the view that oomycetes evolved completely independently from the true fungi. In the phylogenetic tree, P. infestans EF-1alpha forms one branch with EF-1alpha from the unicellular alga Cyanophora paradoxa, an organism belonging to a taxonomic group that occupies a key position in the evolution of plastids.
Assuntos
Fator 1 de Elongação de Peptídeos/genética , Phytophthora/genética , Proteínas de Saccharomyces cerevisiae , Sequência de Aminoácidos , Sequência de Bases , Southern Blotting , DNA Complementar/química , DNA Complementar/genética , DNA Fúngico/genética , Regulação Fúngica da Expressão Gênica , Dados de Sequência Molecular , Filogenia , RNA Fúngico/genética , RNA Fúngico/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
Two in planta-induced (ipi) genes, designated ipiB and ipiO, of the potato late blight fungus, Phytophthora infestans (Mont.) de Bary, were isolated from a genomic library by a differential hybridization procedure [Pieterse et al., Physiol. Mol. Plant Pathol. (1993a) in press]. Both genes are expressed at high levels in the early phases of the pathogenic interaction of P. infestans with its host plant potato, suggesting that their gene products have a function in the early stages of the infection process. Here, we describe the nucleotide (nt) sequence and genomic organization of ipiB and ipiO. The ipiB gene belongs to a small gene family consisting of at least three genes, designated ipiB1, ipiB2 and ipiB3, which are clustered in a head-to-tail arrangement. The three ipiB genes are highly homologous throughout the coding regions and 5' and 3' flanking regions. The P. infestans genome contains two very similar ipiO genes, ipiO1 and ipiO2, which are closely linked and arranged in an inverted orientation. The ipiB genes encode three novel, highly similar Gly-rich proteins of 301, 343 and 347 amino acids (aa), respectively. The Gly-rich domains of the IPI-B proteins are predominantly composed of two repeats with the core sequences, A/V-G-A-G-L-Y-G-R and G-A-G-Y/V-G-G. The ipiO genes code for two almost identical 152-aa proteins which do not have any homology with sequences present in data libraries. IPI-B, as well as IPI-O, contains putative signal peptides of 20 and 21 aa, respectively, suggesting that they are transported out of the cytoplasm. In the promoter regions of ipiB and ipiO, a 16-nt sequence motif, matching the core sequence, GCTCATTYYNCAWTTT (where N = A or C or G or T; W = A or T; Y = C or T), was found. This sequence motif appears to be present around the transcription start point (tsp) of seven out of eight oomycetous genes for which the tsp have been determined, suggesting that oomycetes have a sequence preference for transcription initiation.
Assuntos
Proteínas Fúngicas/biossíntese , Genes Fúngicos , Genoma Fúngico , Família Multigênica , Phytophthora/genética , Sequência de Aminoácidos , Sequência de Bases , Sequência Conservada , DNA Fúngico/genética , DNA Fúngico/isolamento & purificação , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Biblioteca Genômica , Íntrons , Dados de Sequência Molecular , Conformação Proteica , Sequências Repetitivas de Ácido Nucleico , Mapeamento por Restrição , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido NucleicoRESUMO
Here we present the first comprehensive genetic linkage map of the heterothallic oomycetous plant pathogen Phytophthora infestans. The map is based on polymorphic DNA markers generated by the DNA fingerprinting technique AFLP (Vos et al., 1995, Nucleic Acids Res. 23: 4407-4414). AFLP fingerprints were made from single zoospore progeny and 73 F1 progeny from two field isolates of P. infestans. The parental isolates appeared to be homokaryotic and diploid, their AFLP patterns were mitotically stable, and segregation ratios in the F1 progeny were largely Mendelian. In addition to 183 AFLP markers, 7 RFLP markers and the mating type locus were mapped. The linkage map comprises 10 major and 7 minor linkage groups covering a total of 827 cM. The major linkage groups are composed of markers derived from both parents, whereas the minor linkage groups contain markers from either the A1 or the A2 mating type parent. Non-Mendelian segregation ratios were found for the mating type locus and for 13 AFLP markers, all of which are located on the same linkage group as the mating type locus. Copyright 1997 Academic Press
RESUMO
ABSTRACT Natural potato late blight epidemics were studied to assess the relative impact of various inoculum sources of Phytophthora infestans in Southern Flevoland (the Netherlands) from 1994 through 1996. Disease surveys were combined with characterization of isolates for mating type and DNA fingerprint pattern using probe RG57. Seventy-four percent of the commercial potato fields with early foci were clearly associated with nearby infested refuse piles. Characterization of isolates from refuse piles and fields confirmed the association. Infected seed tubers, volunteer plants, and infested allotment gardens appeared to be of minor importance for late blight development in potato fields. Several foci in refuse piles, potato fields, and allotment gardens contained more than one genotype. Due to favorable weather in August 1994, infested organic potato fields became major inoculum sources, resulting in the spread of P. infestans to adjacent conventional potato fields. Analyses of disease gradients, both at the field and regional levels, confirmed the role of the organic fields as mid-season infection sources. The mean slope of field gradients downwind of refuse piles (point sources) was significantly steeper (100-fold difference) than the mean slope of field gradients downwind of organic fields (area sources). The genotypic composition of the P. infestans populations along the gradient and of the source populations in the organic potato crops did not differ significantly. Analysis of the region gradient revealed genotype-specific disease gradients. Control measures are recommended.
RESUMO
ABSTRACT The extracellular protein INF1 of Phytophthora infestans is a member of the elicitin family of protein elicitors known to induce a hypersensitive response on some solanaceous and cruciferous plants. The presence of INF1 elicitin in culture filtrates of 102 P. infestans isolates from 15 countries was examined. All tested isolates produced INF1 except five isolates collected in 1976 and 1977 from infected potatoes in East Germany (the former German Democratic Republic). Based on hybridization to the multi-locus DNA fingerprint probe RG57, all the INF1-nonproducing isolates were shown to belong to the clonal lineage US-1 that dominated world populations until the 1980s. Phylogenetic analysis of a set of European US-1 isolates using amplified fragment length polymorphism fingerprint data indicated that loss of INF1 production evolved independently in separate lineages within US-1. DNA and RNA blot hybridizations showed that INF1-nonproducing isolates still retain a copy of the inf1 gene, whereas little inf1 mRNA could be detected. Hypothetical interpretations of the evolution in a restricted geographic area of P. infestans lineages deficient in the production of a specific elicitor protein are discussed.
RESUMO
Certain characteristics of late noduline gene from pea-Nod6 were investigated as part of works of characterization of higher plant genes taking part in symbiotic nitrogen fixation. The complete 450 b.p. long cDNA was sequenced, it's coding sequence includes the open reading frame. The part of DNA containing the corresponding gene from the genomic clone was also sequenced. The predicted Nod6 amino acid sequence has been analyzed and do not reveal the significant homology with any known protein.
Assuntos
DNA/genética , Fabaceae/genética , Proteínas de Membrana , Fixação de Nitrogênio , Proteínas de Plantas/genética , Plantas Medicinais , Rhizobium/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Southern Blotting , Dados de Sequência Molecular , Fases de Leitura Aberta , Homologia de Sequência do Ácido NucleicoRESUMO
For a comprehensive survey of the structure and dynamics of the Dutch Phytophthora infestans population, 652 P. infestans isolates were collected from commercial potato fields in the Netherlands during the 10-year period 2000-2009. Genotyping was performed using 12 highly informative microsatellite markers and mitochondrial haplotypes. In addition, for each isolate, the mating type was determined. STRUCTURE analysis grouped the 322 identified genotypes in three clusters. Cluster 1 consists of a single clonal lineage NL-001, known as "Blue_13"; all isolates in this cluster have the A2 mating type and the Ia mitochondrial haplotype. Clusters 2 and 3 display a more elaborate substructure containing many unique genotypes. In Cluster 3, several distinct clonal lineages were also identified. This survey witnesses that the Dutch population underwent dramatic changes in the 10 years under study. The most notable change was the emergence and spread of A2 mating type strain NL-001 (or "Blue_13"). The results emphasize the importance of the sexual cycle in generating genetic diversity and the importance of the asexual cycle as the propagation and dispersal mechanism for successful genotypes. Isolates were also screened for absence of the Avrblb1/ipiO class I gene, which is indicative for virulence on Rpi-blb1. This is also the first report of Rpi-blb1 breakers in the Netherlands. Superimposing the virulence screening on the SSR genetic backbone indicates that lack the Avrblb1/ipiO class I gene only occurred in sexual progeny. So far, the asexual spread of the virulent isolates identified has been limited.
Assuntos
Ligação Genética , Phytophthora infestans/genética , Análise por Conglomerados , DNA Mitocondrial/genética , Genótipo , Haplótipos , Repetições de Microssatélites , Países Baixos , Phytophthora infestans/patogenicidade , Polimorfismo Genético , Dinâmica Populacional , Solanum tuberosum/parasitologia , Virulência/genéticaRESUMO
This article documents the addition of 396 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Anthocidaris crassispina, Aphis glycines, Argyrosomus regius, Astrocaryum sciophilum, Dasypus novemcinctus, Delomys sublineatus, Dermatemys mawii, Fundulus heteroclitus, Homalaspis plana, Jumellea rossii, Khaya senegalensis, Mugil cephalus, Neoceratitis cyanescens, Phalacrocorax aristotelis, Phytophthora infestans, Piper cordulatum, Pterocarpus indicus, Rana dalmatina, Rosa pulverulenta, Saxifraga oppositifolia, Scomber colias, Semecarpus kathalekanensis, Stichopus monotuberculatus, Striga hermonthica, Tarentola boettgeri and Thermophis baileyi. These loci were cross-tested on the following species: Aphis gossypii, Sooretamys angouya, Euryoryzomys russatus, Fundulus notatus, Fundulus olivaceus, Fundulus catenatus, Fundulus majalis, Jumellea fragrans, Jumellea triquetra Jumellea recta, Jumellea stenophylla, Liza richardsonii, Piper marginatum, Piper aequale, Piper darienensis, Piper dilatatum, Rana temporaria, Rana iberica, Rana pyrenaica, Semecarpus anacardium, Semecarpus auriculata, Semecarpus travancorica, Spondias acuminata, Holigarna grahamii, Holigarna beddomii, Mangifera indica, Anacardium occidentale, Tarentola delalandii, Tarentola caboverdianus and Thermophis zhaoermii.
RESUMO
The expression of plant genes involved in the pea-Rhizobium symbiosis was studied by analysing mRNA from root nodules. The RNA was translated in vitro and the translation products were separated by two-dimensional gel electrophoresis. The results show differential expression of nodulin genes during root nodule development. One gene encoding N-40' is expressed at a significant level 5 days before the leghemoglobin genes. Most other nodulin genes are expressed more of less concomitantly with the leghemoglobin genes whereas the N-21 mRNA is only present late during the development. In the development of ineffective root nodules induced by infection with different nod+fix- mutants of R. leguminosarum all nodulin genes are expressed except for the N-21 gene. The results suggest that neither bacteroid development, heme excretion nor nitrogen fixation are essential for the induction of nodulin gene expression in the host plant. Further, it appears that the amount of leghemoglobin in ineffective nodules is regulated at a post-transcriptional level.