Lifestyle exercise attenuates immunosenescence; flow cytometry analysis.

BACKGROUND: Interaction of physical activity and overall immune profile is very complex and depends on the intensity, duration and frequency of undertaken physical activity, the exposure to cytomegalovirus (CMV) infection and the age-related changes in the immune system. Daily physical activity, which particularly influences immunity, declines dramatically with age. Therefore, the aim of the study was to explain whether physical activity sustained throughout life can attenuate or reverse immunosenescence. METHODS: Ninety-nine older adults (60-90 years) were recruited for the study. According to the 6-min walk test (6WMT), the Åstrand-Ryhming bike test (VO2max) and Community Healthy Activities Model Program for Seniors (CHAMPS) questionnaire, the individuals were classified as physically active (n = 34) and inactive (n = 20) groups. The analysis of T lymphocytes between active vs. inactive participants was performed using eight-parameter flow cytometry. RESULTS: Analysis of the baseline peripheral naïve and memory T lymphocytes showed a significant relationship of lifestyle exercise with the CD4/CD8 ratio. Above 50% of physically active participants demonstrated the CD4/CD8 ratio ≥ 1 or ≤ 2.5 contrary to the inactive group who showed the ratio < 1. The older adults with the result of 6WMT > 1.3 m/s and VO2max > 35 mL/kg/min had a significantly higher CD4+CD45RA+ T lymphocyte percentage and also a higher ratio of CD4+CD45RA+/CD4+CD45RO+. Interestingly, in active older adults with IgG CMV+ (n = 30) the count of CD4+CD45RA+ T lymphocytes was higher than in the inactive group with IgG CMV+ (n = 20). CONCLUSION: Based on the flow cytometry analysis, we concluded that lifestyle exercise could lead to rejuvenation of the immune system by increasing the percentage of naïve T lymphocytes or by reducing the tendency of the inverse CD4/CD8 ratio.

Old wine into new wineskins? "Legacy data" in research on Roman Period East Germanic iron smelting.

This paper discusses the use of "legacy data" in research on Roman Period iron smelting in the territory of the Przeworsk Culture in Magna Germania. The dataset includes results of 240 analyses of smelting slag and iron ores chemistry. A majority of these analyses were conducted in the 1950s-1980s. The quality of these data is far below present-day standards. Only major elements were reported, analytical methods were often not specified (although optical emission spectroscopy and wet chemical analyses can be assumed in such cases) and information on detection limits, precision and accuracy of the results is missing. In spite of this, a Principal Component Analysis-Agglomerative Hierarchical Clustering treatment successfully isolated observations from the three main iron smelting regions of the Przeworsk Culture (the Holy Cross Mountains, Masovia and Silesia). These results to a degree confirm a theory proposed in the 1960s by J. Piaskowski, according to whom it was possible to distinguish iron produced in the Holy Cross Mountains from the iron produced elsewhere in the territory of what is now Poland on the basis of metal characteristics. These findings will pave the way to the ongoing research project on the Przeworsk Culture metallurgy. It is also argued that, apart from a search for new methods in iron provenance studies, more attention should be paid to results of earlier analyses and to a compilation of legacy databases. The other result is an open and flexible Agglomerative Hierarchical Clustering R code to examine discrimination between production areas and to propose artefact provenance patterns in a convenient interactive way using the R development environment.

A deep learning framework for epileptic seizure detection based on neonatal EEG signals.

Electroencephalogram (EEG) is one of the main diagnostic tests for epilepsy. The detection of epileptic activity is usually performed by a human expert and is based on finding specific patterns in the multi-channel electroencephalogram. This is a difficult and time-consuming task, therefore various attempts are made to automate it using both conventional and Deep Learning (DL) techniques. Unfortunately, authors do not often provide sufficiently detailed and complete information to be able to reproduce their results. Our work is intended to fill this gap. Using a carefully selected 79 neonatal EEG recordings we developed a complete framework for seizure detection using DL approch. We share a ready to use R and Python codes which allow: (a) read raw European Data Format files, (b) read data files containing the seizure annotations made by human experts, (c) extract train, validation and test data, (d) create an appropriate Convolutional Neural Network (CNN) model, (e) train the model, (f) check the quality of the neural classifier, (g) save all learning results.

Pre-Existing Hypertension Is Related with Disproportions in T-Lymphocytes in Older Age.

Age-related immune deficiencies increase the risk of comorbidities and mortality. This study evaluated immunosenescence patterns by flow cytometry of naïve and memory T cell subpopulations and the immune risk profile (IRP), expressed as the CD4/CD8 ratio and IgG CMV related to comorbidities. The disproportions in naïve and memory T cells, as well as in the CD4/CD8 ratio, were analysed in 99 elderly individuals (71.9 ± 5.8 years) diagnosed with hypertension (n = 51) or without hypertension (n = 48), using an eight-parameter flow cytometer. The percentage of CD4+ T lymphocytes was significantly higher in hypertensive than other individuals independently from CMV infections, with approximately 34% having CD4/CD8 > 2.5, and only 4% of the elderly with hypertension having CD4/CD8 < 1. The elderly with a normal BMI demonstrated the CD4/CD8 ratio ≥ 1 or ≤ 2.5, while overweight and obese participants showed a tendency to an inverted CD4/CD8 ratio. CD4/CD8 ratio increased gradually with age and reached the highest values in participants aged >75 years. The decline in CD4+ naïve T lymphocytes was more prominent in IgG CMV+ men when compared to IgG CMV+ women. The changes in naïve and memory T lymphocyte population, CD4/CD8, and CMV seropositivity included in IRP are important markers of health status in the elderly that are dependent on hypertension.

Intermittent Hypoxic Exposure with High Dose of Arginine Impact on Circulating Mediators of Tissue Regeneration.

Intermittent exposure to hypoxia (IHE) increases production of reactive oxygen and nitrogen species which, as signalling molecules, participate in tissue injury-repair-regeneration cascade. The process is also stimulated by arginine whose bioavailability is a limiting factor for NO synthesis. The effects of IHE in combination with arginine (Arg) intake on myogenesis and angiogenesis mediators were examined in a randomized and placebo-controlled trial. Blood samples were collected from 38 elite athletes on the 1st, 7th and 14th days during the training camp. The oral doses of arginine (2 × 6 g/day) and/or IHE using hypoxicator GO2Altitude (IHE and Arg/IHE) were applied. Serum NO and H2O2 concentrations increased significantly and were related to muscle damage (CK activity >900 IU/mL) in IHE and Arg/IHE compared to placebo. The changes in NO and H2O2 elevated the levels of circulating growth factors such as HGF, IHG-1, PDGFBB, BDNF, VEGF and EPO. Modification of the lipid profile, especially reduced non-HDL, was an additional beneficial effect of hypoxic exposure with arginine intake. Intermittent hypoxic exposure combined with high-dose arginine intake was demonstrated to affect circulating mediators of injury-repair-regeneration. Therefore, a combination of IHE and arginine seems to be a potential therapeutic and non-pharmacological method to modulate the myogenesis and angiogenesis in elite athletes.