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1.
Annu Rev Entomol ; 69: 21-40, 2024 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-37562048

RESUMO

The evolution of sexual communication is critically important in the diversity of arthropods, which are declining at a fast pace worldwide. Their environments are rapidly changing, with increasing chemical, acoustic, and light pollution. To predict how arthropod species will respond to changing climates, habitats, and communities, we need to understand how sexual communication systems can evolve. In the past decades, intraspecific variation in sexual signals and responses across different modalities has been identified, but never in a comparative way. In this review, we identify and compare the level and extent of intraspecific variation in sexual signals and responses across three different modalities, chemical, acoustic, and visual, focusing mostly on insects. By comparing causes and possible consequences of intraspecific variation in sexual communication among these modalities, we identify shared and unique patterns, as well as knowledge needed to predict the evolution of sexual communication systems in arthropods in a changing world.


Assuntos
Artrópodes , Animais , Comunicação
2.
J Chem Ecol ; 2024 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-38421546

RESUMO

The fall armyworm, Spodoptera frugiperda (J.E. Smith), is a global pest that feeds on > 350 plant species and causes major yield loses. Variation in the responses of S. frugiperda males to female sex pheromone compounds affects the detection, monitoring and management of the pest. We determined geographic variation in the responses of S. frugiperda males to four different doses of synthetic sex pheromone compounds using a gas chromatography-electroantennogram detector (GC-EAD). Furthermore, we disentangled regional populations into C- and R- mitotypes via molecular analysis of the cytochrome oxidase I gene, and measured their responses to the compounds. When comparing responses of males from Florida, Benin, Nigeria and Kenya, we found some regional differences in the responses of S. frugiperda males to the major compound, Z9-14:OAc and minor component Z9-12:OAc. However, we found no differences in male responses between the different African countries. All males showed significantly higher antennal responses to Z7-12:OAc than to E7-12:OAc. When comparing the mitotypes, we found that Florida R-type males showed higher responses to Z9-14:OAc, Z7-12:OAc and Z9-12:OAc than Benin R-type males, while C-type males from both regions responded equally to Z7-12:OAc. In addition, Florida R-type males showed higher responses to E7-12:OAc than Florida C-type males. Our study thus shows some differential physiological responses of S. frugiperda males towards the known sex pheromone compounds, including E7-12:OAc, but mostly in the different mitotypes. How these differences translate to field trap catches remains to be determined.

3.
Annu Rev Entomol ; 68: 299-317, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36198399

RESUMO

The fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera, Noctuidae), is a well-known agricultural pest in its native range, North and South America, and has become a major invasive pest around the globe in the past decade. In this review, we provide an overview to update what is known about S. frugiperda in its native geographic ranges. This is followed by discussion of studies from the invaded areas to gain insights into S. frugiperda's ecology, specifically its reproductive biology, host plant use, status of insecticide resistance alleles, and biocontrol methods in native and invasive regions. We show that reference to host strains is uninformative in the invasive populations because multidirectional introduction events likely underpinned its recent rapid spread. Given that recent genomic analyses show that FAW is much more diverse than was previously assumed, and natural selection forces likely differ geographically, region-specific approaches will be needed to control this global pest.


Assuntos
Agricultura , Espécies Introduzidas , Animais , Spodoptera/genética , América do Sul , Ecologia
4.
J Evol Biol ; 36(5): 780-794, 2023 05.
Artigo em Inglês | MEDLINE | ID: mdl-37026574

RESUMO

Chemical communication is ubiquitous in nature and chemical signals convey species-specific messages. Despite their specificity, chemical signals may not be limited to only one function. Identifying alternative functions of chemical signals is key to understanding how chemical communication systems evolve. Here, we explored alternative functions of moth sex pheromone compounds. These chemicals are generally produced in, and emitted from, dedicated sex pheromone glands, but some have recently also been found on the insects' legs. We identified and quantified the chemicals in leg extracts of the three heliothine moth species Chloridea (Heliothis) virescens, Chloridea (Heliothis) subflexa and Helicoverpa armigera, compared their chemical profiles and explored the biological function of pheromone compounds on moth legs. Identical pheromone compounds were present on the legs in both sexes of all three species, with no striking interspecies or intersex differences. Surprisingly, we also found pheromone-related acetate esters in leg extracts of species that lack acetate esters in their female sex pheromone. When we assessed gene expression levels in the leg tissue, we found known and putative pheromone-biosynthesis genes expressed, which suggests that moth legs may be additional sites of pheromone production. To determine possible additional roles of the pheromone compounds on legs, we explored whether these may act as oviposition-deterring signals, which does not seem to be the case. However, when we tested whether these chemicals have antimicrobial properties, we found that two pheromone compounds (16:Ald and 16:OH) reduce bacterial growth. Such an additional function of previously identified pheromone compounds likely coincides with additional selection pressures and, thus, should be considered in scenarios on the evolution of these signals.


Assuntos
Mariposas , Atrativos Sexuais , Masculino , Animais , Feminino , Feromônios , Atrativos Sexuais/farmacologia , Mariposas/genética , Especificidade da Espécie , Ésteres/metabolismo
5.
RNA Biol ; 20(1): 48-58, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36727270

RESUMO

Automated genome annotation is essential for extracting biological information from sequence data. The identification and annotation of tRNA genes is frequently performed by the software package tRNAscan-SE, the output of which is listed for selected genomes in the Genomic tRNA database (GtRNAdb). Here, we highlight a pervasive error in prokaryotic tRNA gene sets on GtRNAdb: the mis-categorization of partial, non-canonical tRNA genes as standard, canonical tRNA genes. Firstly, we demonstrate the issue using the tRNA gene sets of 20 organisms from the archaeal taxon Thermococcaceae. According to GtRNAdb, these organisms collectively deviate from the expected set of tRNA genes in 15 instances, including the listing of eleven putative canonical tRNA genes. However, after detailed manual annotation, only one of these eleven remains; the others are either partial, non-canonical tRNA genes resulting from the integration of genetic elements or CRISPR-Cas activity (seven instances), or attributable to ambiguities in input sequences (three instances). Secondly, we show that similar examples of the mis-categorization of predicted tRNA sequences occur throughout the prokaryotic sections of GtRNAdb. While both canonical and non-canonical prokaryotic tRNA gene sequences identified by tRNAscan-SE are biologically interesting, the challenge of reliably distinguishing between them remains. We recommend employing a combination of (i) screening input sequences for the genetic elements typically associated with non-canonical tRNA genes, and ambiguities, (ii) activating the tRNAscan-SE automated pseudogene detection function, and (iii) scrutinizing predicted tRNA genes with low isotype scores. These measures greatly reduce manual annotation efforts, and lead to improved prokaryotic tRNA gene set predictions.


Assuntos
Genoma , RNA de Transferência , RNA de Transferência/genética
6.
BMC Evol Biol ; 20(1): 53, 2020 05 07.
Artigo em Inglês | MEDLINE | ID: mdl-32380947

RESUMO

BACKGROUND: Ever since Darwin, evolutionary biologists have studied sexual selection driving differences in appearance and behaviour between males and females. An unchallenged paradigm in such studies is that one sex (usually the male) signals its quality as a mate to the other sex (usually the female), who is choosy in accepting a partner. Here, we hypothesize that in polygamous species these roles change dynamically with the mating status of males and females, depending on direct reproductive costs and benefits of multiple matings, and on sperm competition. We test this hypothesis by assessing fitness costs and benefits of multiple matings in both males and females in a polygamous moth species, as in moths not males but females are the signalers and males are the responders. RESULTS: We found that multiple matings confer fitness costs and benefits for both sexes. Specifically, the number of matings did not affect the longevity of males or females, but only 67% of the males and 14% of the females mated successfully in all five nights. In addition, the female's reproductive output increased with multiple matings, although when paired with a new virgin male every night, more than 3 matings decreased her reproductive output, so that the Bateman gradient for females fit a quadratic model better than a linear model. The male's reproductive success was positively affected by the number of matings and a linear regression line best fit the data. Simulations of the effect of sperm competition showed that increasing last-male paternity increases the steepness of the male Bateman gradient and thus the male's relative fitness gain from additional mating. Irrespective of last-male paternity value, the female Bateman gradient is steeper than the male one for up to three matings. CONCLUSION: Our results suggest that choosiness in moths may well change throughout the mating season, with males being more choosy early in the season and females being more choosy after having mated at least three times. This life-history perspective on the costs and benefits of multiple matings for both sexes sheds new light on sexual selection forces acting on sexual signals and responses.


Assuntos
Mariposas/fisiologia , Comportamento Sexual Animal/fisiologia , Animais , Evolução Biológica , Feminino , Longevidade , Masculino , Reprodução/fisiologia , Especificidade da Espécie , Espermatozoides/metabolismo
7.
J Invertebr Pathol ; 170: 107328, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31952966

RESUMO

Many parasites are constrained to only one or a few hosts, showing host specificity. It remains unclear why some parasites are specialists and other parasites are generalists. The parasite Ophryocystis elektroscirrha (OE) is a neogregarine protozoan thought to be restricted to monarch butterflies, Danaus plexippus (Nymphaliae) and D. gilippus. Recently, we found OE-like spores in other Lepidoptera, specifically in three noctuid moths: Helicoverpa armigera, H. assulta and H. punctigera, as well as another nymphalid, Parthenos sylvia. To our knowledge, this is the first report of OE-like parasite infections in species other than the genus Danaus. In sequencing 558 bp of 18S rRNA, we found the genetic similarity between OE from D. plexippus and OE-like parasite from the moths H. armigera and H. punctigera to be 95.2%. When we conducted cross-species infection experiments, we could not infect the moths with OE from D. plexippus, but OE-like parasite from H. armigera did infect D. plexippus and a closely related moth species Heliothis virescens. Interestingly, we did not find the OE-like parasite in the H. armigera population from Spain. Inter-population infection experiments with H. armigera demonstrated a higher sensitivity to OE-like infection in the population from Spain compared to the populations from Australia and China. These results suggest geographic variation in OE-like susceptibility and coevolution between parasite and host. Our findings give important new insights into the prevalence and host specificity of OE and OE-like parasites, and provide opportunities to study parasite transmission over spatial and temporal scales.


Assuntos
Apicomplexa/fisiologia , Borboletas/parasitologia , Especificidade de Hospedeiro , Interações Hospedeiro-Parasita , Mariposas/parasitologia , Animais , Borboletas/crescimento & desenvolvimento , Larva/crescimento & desenvolvimento , Larva/parasitologia , Mariposas/crescimento & desenvolvimento , Pupa/crescimento & desenvolvimento , Pupa/parasitologia , Especificidade da Espécie
8.
J Evol Biol ; 32(8): 754-768, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31215105

RESUMO

In an ideal world, funding agencies could identify the best scientists and projects and provide them with the resources to undertake these projects. Most scientists would agree that in practice, how funding for scientific research is allocated is far from ideal and likely compromises research quality. We, nine evolutionary biologists from different countries and career stages, provide a comparative summary of our impressions on funding strategies for evolutionary biology across eleven different funding agencies. We also assess whether and how funding effectiveness might be improved. We focused this assessment on 14 elements within four broad categories: (a) topical shaping of science, (b) distribution of funds, (c) application and review procedures, and (d) incentives for mobility and diversity. These comparisons revealed striking among-country variation in those elements, including wide variation in funding rates, the effort and burden required for grant applications, and the extent of emphasis on societal relevance and individual mobility. We use these observations to provide constructive suggestions for the future and urge the need to further gather informed considerations from scientists on the effects of funding policies on science across countries and research fields.


Assuntos
Evolução Biológica , Financiamento de Capital , Apoio à Pesquisa como Assunto , Ciência/economia , Política Pública , Estados Unidos
9.
Proc Natl Acad Sci U S A ; 113(42): E6401-E6408, 2016 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-27698145

RESUMO

The sexual pheromone communication system of moths is a model system for studies of the evolution of reproductive isolation. Females emit a blend of volatile components that males detect at a distance. Species differences in female pheromone composition and male response directly reinforce reproductive isolation in nature, because even slight variations in the species-specific pheromone blend are usually rejected by the male. The mechanisms by which a new pheromone signal-response system could evolve are enigmatic, because any deviation from the optimally attractive blend should be selected against. Here we investigate the genetic mechanisms enabling a switch in male response. We used a quantitative trait locus-mapping approach to identify the genetic basis of male response in the two pheromone races of the European corn borer, Ostrinia nubilalis Male response to a 99:1 vs. a 3:97 ratio of the E and Z isomers of the female pheromone is governed by a single, sex-linked locus. We found that the chromosomal region most tightly linked to this locus contains genes involved in neurogenesis but, in accordance with an earlier study, does not contain the odorant receptors expressed in the male antenna that detect the pheromone. This finding implies that differences in the development of neuronal pathways conveying information from the antenna, not differences in pheromone detection by the odorant receptors, are primarily responsible for the behavioral response differences among the males in this system. Comparison with other moth species reveals a previously unexplored mechanism by which male pheromone response can change in evolution.


Assuntos
Mapeamento Cromossômico , Genes de Insetos , Estudos de Associação Genética , Mariposas/fisiologia , Neurogênese/genética , Feromônios , Análise de Variância , Animais , Comportamento Animal , Cruzamentos Genéticos , Feminino , Ligação Genética , Escore Lod , Masculino , Locos de Características Quantitativas , Atrativos Sexuais
10.
New Phytol ; 220(3): 739-749, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-28256726

RESUMO

Chemical communication is ubiquitous. The identification of conserved structural elements in visual and acoustic communication is well established, but comparable information on chemical communication displays (CCDs) is lacking. We assessed the phenotypic integration of CCDs in a meta-analysis to characterize patterns of covariation in CCDs and identified functional or biosynthetically constrained modules. Poorly integrated plant CCDs (i.e. low covariation between scent compounds) support the notion that plants often utilize one or few key compounds to repel antagonists or to attract pollinators and enemies of herbivores. Animal CCDs (mostly insect pheromones) were usually more integrated than those of plants (i.e. stronger covariation), suggesting that animals communicate via fixed proportions among compounds. Both plant and animal CCDs were composed of modules, which are groups of strongly covarying compounds. Biosynthetic similarity of compounds revealed biosynthetic constraints in the covariation patterns of plant CCDs. We provide a novel perspective on chemical communication and a basis for future investigations on structural properties of CCDs. This will facilitate identifying modules and biosynthetic constraints that may affect the outcome of selection and thus provide a predictive framework for evolutionary trajectories of CCDs in plants and animals.


Assuntos
Evolução Biológica , Vias Biossintéticas , Animais , Fenótipo , Compostos Orgânicos Voláteis/metabolismo
11.
J Chem Ecol ; 44(7-8): 621-630, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30039209

RESUMO

Female-emitted volatile sex pheromones in most moths are composed of biosynthetically related blends of fatty acid derivatives, such as aldehydes, acetate esters and alcohols. In many moths, as in the noctuid Heliothis (Chloridea) virescens, the pheromone gland contains alcohols (e.g., (Z)-11-hexadecen-1-ol, hereafter Z11-16:OH) that may serve dual functions as pheromone components as well as precursors of other pheromone components. The relative importance of Z11-16:OH to male attraction in H. virescens has been controversial. It occurs in the pheromone gland in relatively large amounts, but several studies could neither detect Z11-16:OH in gland emissions nor attribute any conspecific behavioral function to it in flight- tunnel assays. Trapping assays in the field, however, have more consistently documented that the addition of Z11-16:OH increased trap catch. Using a short section of thick film megabore column, in combination with derivatization and GC-CI-SIM-MS, we determined that Z11-16:OH is emitted from the sex pheromone gland during calling. Field trapping studies demonstrated that trap catch increased when Z11-16:OH was added to a 2-component minimal blend and to a 6-component blend. Behavioral observations in the field confirmed that more males responded to a pheromone blend that contained a low blend ratio of Z11-16:OH, but ≥5% Z11-16:OH depressed both male behavior and trap catch. We conclude that Z11-16:OH should be considered a component of the sex pheromone of H. virescens females.


Assuntos
Álcoois Graxos/metabolismo , Mariposas/fisiologia , Atrativos Sexuais/metabolismo , Comportamento Sexual Animal , Comunicação Animal , Animais , Álcoois Graxos/análise , Feminino , Masculino , Mariposas/química , Atrativos Sexuais/análise , Compostos Orgânicos Voláteis/análise , Compostos Orgânicos Voláteis/metabolismo
12.
BMC Evol Biol ; 17(1): 68, 2017 03 06.
Artigo em Inglês | MEDLINE | ID: mdl-28264650

RESUMO

BACKGROUND: Very little is known on how changes in circadian rhythms evolve. The noctuid moth Spodoptera frugiperda (Lepidoptera: Noctuidae) consists of two strains that exhibit allochronic differentiation in their mating time, which acts as a premating isolation barrier between the strains. We investigated the genetic basis of the strain-specific timing differences to identify the molecular mechanisms of differentiation in circadian rhythms. RESULTS: Through QTL analyses we identified one major Quantitative trait chromosome (QTC) underlying differentiation in circadian timing of mating activity. Using RADtags, we identified this QTC to be homologous to Bombyx mori C27, on which the clock gene vrille is located, which thus became the major candidate gene. In S. frugiperda, vrille showed strain-specific polymorphisms. Also, vrille expression differed significantly between the strains, with the rice-strain showing higher expression levels than the corn-strain. In addition, RT-qPCR experiments with the other main clock genes showed that pdp1, antagonist of vrille in the modulatory feedback loop of the circadian clock, showed higher expression levels in the rice-strain than in the corn-strain. CONCLUSIONS: Together, our results indicate that the allochronic differentiation in the two strains of S. frugiperda is associated with differential transcription of vrille or a cis-acting gene close to vrille, which contributes to the evolution of prezygotic isolation in S. frugiperda.


Assuntos
Genes de Insetos , Spodoptera/genética , Animais , Ritmo Circadiano , Larva/genética , Oryza , Polimorfismo Genético , Reprodução , Estações do Ano , Spodoptera/classificação , Spodoptera/crescimento & desenvolvimento , Spodoptera/fisiologia , Zea mays
13.
Int J Mol Sci ; 18(1)2017 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-28106771

RESUMO

It is well known that microbial pathogens and herbivores elicit defence responses in plants. Moreover, microorganisms associated with herbivores, such as bacteria or viruses, can modulate the plant's response to herbivores. Herbivorous spider mites can harbour different species of bacterial symbionts and exert a broad range of effects on host-plant defences. Hence, we tested the extent to which such symbionts affect the plant's defences induced by their mite host and assessed if this translates into changes in plant resistance. We assessed the bacterial communities of two strains of the common mite pest Tetranychus urticae. We found that these strains harboured distinct symbiotic bacteria and removed these using antibiotics. Subsequently, we tested to which extent mites with and without symbiotic bacteria induce plant defences in terms of phytohormone accumulation and defence gene expression, and assessed mite oviposition and survival as a measure for plant resistance. We observed that the absence/presence of these bacteria altered distinct plant defence parameters and affected mite performance but we did not find indications for a causal link between the two. We argue that although bacteria-related effects on host-induced plant defences may occur, these do not necessarily affect plant resistance concomitantly.


Assuntos
Bactérias/efeitos dos fármacos , Herbivoria , Solanum lycopersicum/microbiologia , Solanum lycopersicum/parasitologia , Simbiose , Tetranychidae/fisiologia , Animais , Antibacterianos/farmacologia , Feminino , Herbivoria/efeitos dos fármacos , Solanum lycopersicum/imunologia , Masculino , Movimento , Reguladores de Crescimento de Plantas/análise , Análise de Sobrevida , Simbiose/efeitos dos fármacos , Tetranychidae/efeitos dos fármacos
14.
Annu Rev Entomol ; 61: 99-117, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26565898

RESUMO

Moth sexual pheromones are widely studied as a fine-tuned system of intraspecific sexual communication that reinforces interspecific reproductive isolation. However, their evolution poses a dilemma: How can the female pheromone and male preference simultaneously change to create a new pattern of species-specific attraction? Solving this puzzle requires us to identify the genes underlying intraspecific variation in signals and responses and to understand the evolutionary mechanisms responsible for their interspecific divergence. Candidate gene approaches and functional analyses have yielded insights into large families of biosynthetic enzymes and pheromone receptors, although the factors controlling their expression remain largely unexplored. Intra- and interspecific crosses have provided tantalizing evidence of regulatory genes, although, to date, mapping resolution has been insufficient to identify them. Recent advances in high-throughput genome and transcriptome sequencing, together with established techniques, have great potential to help scientists identify the specific genetic changes underlying divergence and resolve the mystery of how moth sexual communication systems evolve.


Assuntos
Evolução Biológica , Proteínas de Insetos/genética , Mariposas/fisiologia , Atrativos Sexuais/genética , Animais , Feminino , Proteínas de Insetos/metabolismo , Masculino , Mariposas/genética , Atrativos Sexuais/metabolismo , Especificidade da Espécie
15.
Nature ; 466(7305): 486-9, 2010 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-20592730

RESUMO

Pheromone-based behaviours are crucial in animals from insects to mammals, and reproductive isolation is often based on pheromone differences. However, the genetic mechanisms by which pheromone signals change during the evolution of new species are largely unknown. In the sexual communication system of moths (Insecta: Lepidoptera), females emit a species-specific pheromone blend that attracts males over long distances. The European corn borer, Ostrinia nubilalis, consists of two sex pheromone races, Z and E, that use different ratios of the cis and trans isomers of acetate pheromone components. This subtle difference leads to strong reproductive isolation in the field between the two races, which could represent a first step in speciation. Female sex pheromone production and male behavioural response are under the control of different major genes, but the identity of these genes is unknown. Here we show that allelic variation in a fatty-acyl reductase gene essential for pheromone biosynthesis accounts for the phenotypic variation in female pheromone production, leading to race-specific signals. Both the cis and trans isomers of the pheromone precursors are produced by both races, but the precursors are differentially reduced to yield opposite ratios in the final pheromone blend as a result of the substrate specificity of the enzymes encoded by the Z and E alleles. This is the first functional characterization of a gene contributing to intraspecific behavioural reproductive isolation in moths, highlighting the importance of evolutionary diversification in a lepidopteran-specific family of reductases. Accumulation of substitutions in the coding region of a single biosynthetic enzyme can produce pheromone differences resulting in reproductive isolation, with speciation as a potential end result.


Assuntos
Alelos , Mariposas/fisiologia , Oxirredutases/genética , Oxirredutases/metabolismo , Atrativos Sexuais/metabolismo , Animais , Feminino , Isomerismo , Masculino , Dados de Sequência Molecular , Mariposas/classificação , Mariposas/enzimologia , Mariposas/genética , Filogenia , RNA/análise , RNA/genética , RNA/metabolismo , Atrativos Sexuais/biossíntese , Atrativos Sexuais/química , Especificidade por Substrato
16.
BMC Evol Biol ; 15: 282, 2015 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-26672978

RESUMO

BACKGROUND: Immune response induction benefits insects in combatting infection by pathogens. However, organisms have a limited amount of resources available and face the dilemma of partitioning resources between immunity and other life-history traits. Since males and females differ in their life histories, sex-specific resource investment strategies to achieve an optimal immune response following an infection can be expected. We investigated immune response induction of females and males of Heliothis virescens in response to the entomopathogenic bacterium Serratia entomophila, and its effects on mating success and the female sexual signal. RESULTS: We found that females had higher expression levels of immune-related genes after bacterial challenge than males. However, males maintained a higher baseline expression of immune-related genes than females. The increased investment in immunity of female moths was negatively correlated with mating success and the female sexual signal. Male mating success was unaffected by bacterial challenge. CONCLUSIONS: Our results show that the sexes differed in their investment strategies: females invested in immune defense after a bacterial challenge, indicating facultative immune deployment, whereas males had higher baseline immunity than females, indicating immune maintenance. Interestingly, these differences in investment were reflected in the mate choice assays. As female moths are the sexual signallers, females need to invest resources in their attractiveness. However, female moths appeared to invest in immunity at the cost of reproductive effort.


Assuntos
Mariposas/imunologia , Mariposas/fisiologia , Serratia/fisiologia , Animais , Feminino , Estágios do Ciclo de Vida , Masculino , Mariposas/crescimento & desenvolvimento , Mariposas/microbiologia , Reprodução , Caracteres Sexuais , Transcriptoma
17.
J Chem Ecol ; 41(12): 1155-62, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26625875

RESUMO

The legume pod borer, Maruca vitrata, is a pantropical pest on leguminous crops. (E,E)-10,12-Hexadecadienal, (E,E)-10,12-hexadecadienol, and (E)-10-hexadecenal were described previously as sex pheromone components for this nocturnal moth. A blend of these components in a ratio of 100:5:5 attracted males in field trapping experiments in Benin, but not in Taiwan, Thailand, or Vietnam. This finding suggests geographic variation in the pheromone blend between Asian and West African populations of M. vitrata. We, therefore, determined the pheromone compositions of single pheromone glands of females from the three Asian regions and from Benin by gas chromatography-mass spectrometry. Additionally, we compared the responses of males from Taiwan and Benin to calling females and to gland extracts of females from both regions in laboratory no-choice and two-choice assays. Chemical analysis revealed the presence of (E,E)-10,12-hexadecadienal and (E,E)-10,12-hexadecadienol, as well as the absence of (E)-10-hexadecenal in all four populations. The relative amounts of the detected compounds did not vary significantly among the insect populations. The behavioral bioassays showed that Taiwanese and Beninese males were similarly attracted to females from both regions, as well as to their gland extracts. As a result, we did not find geographic variation in the sexual communication system of M. vitrata between West African and Asian insect populations.


Assuntos
Mariposas/fisiologia , Atrativos Sexuais/metabolismo , Animais , Benin , Quimiotaxia , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Masculino , Taiwan
18.
Proc Biol Sci ; 281(1788): 20140897, 2014 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-24943370

RESUMO

Ecological immunology examines the adaptive responses of animals to pathogens in relation to other environmental factors and explores the consequences of trade-offs between investment in immune function and other life-history traits. Among species of herbivorous insects, diet breadth may vary greatly, with generalists consuming a wide variety of plant families and specialists restricted to a few species. Generalists may thus be exposed to a wider range of pathogens exerting stronger selection on the innate immune system. To examine whether this produces an increase in the robustness of the immune response, we compared larvae of the generalist herbivore Heliothis virescens and the specialist Heliothis subflexa challenged by entomopathogenic and non-pathogenic bacteria. Heliothis virescens larvae showed lower mortality, a lower number of recoverable bacteria, lower proliferation of haemocytes and higher phagocytic activity. These results indicate a higher tolerance to entomopathogenic bacteria by the generalist, which is associated with a more efficient cell-mediated immune response by mechanisms that differ between these closely related species. Our findings provide novel insights into the consequences of diet breadth and related environmental factors, which may be significant in further studies to understand the ecological forces and investment trade-offs that shape the evolution of innate immunity.


Assuntos
Bacillus subtilis/fisiologia , Bacillus thuringiensis/fisiologia , Mariposas/fisiologia , Serratia/fisiologia , Animais , Dieta , Larva/imunologia , Larva/microbiologia , Larva/fisiologia , Mariposas/crescimento & desenvolvimento , Mariposas/imunologia , Mariposas/microbiologia , Especificidade da Espécie
19.
Proc Biol Sci ; 281(1779): 20133054, 2014 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-24500170

RESUMO

Evolutionary diversification of sexual communication systems in moths is perplexing because signal and response are under stabilizing selection in many species, and this is expected to constrain evolutionary change. In the moth Heliothis virescens, we consistently found high phenotypic variability in the female sex pheromone blend within each of four geographically distant populations. Here, we assess the heritability, genetic basis and behavioural consequences of this variation. Artificial selection with field-collected moths dramatically increased the relative amount of the saturated compound 16:Ald and decreased its unsaturated counterpart Z11-16:Ald, the major sex pheromone component (high line). In a cross between the high- and low-selected lines, one quantitative trait locus (QTL) explained 11-21% of the phenotypic variance in the 16:Ald/Z11-16:Ald ratio. Because changes in activity of desaturase enzymes could affect this ratio, we measured their expression levels in pheromone glands and mapped desaturase genes onto our linkage map. A delta-11-desaturase had lower expression in females producing less Z11-16:Ald; however, this gene mapped to a different chromosome than the QTL. A model in which the QTL is a trans-acting repressor of delta-11 desaturase expression explains many features of the data. Selection favouring heterozygotes which produce more unsaturated components could maintain a polymorphism at this locus.


Assuntos
Mariposas/genética , Atrativos Sexuais/genética , Comportamento Sexual Animal , Animais , Variação Genética , Endogamia , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Mariposas/metabolismo , Mariposas/fisiologia , Fenótipo , Locos de Características Quantitativas , Atrativos Sexuais/química
20.
Insect Sci ; 2024 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-38769890

RESUMO

Given the limited availability of resources in nature, sexual attractiveness may trade off with immunocompetence, as the immunocompetence handicap hypothesis (ICHH) posits. In invertebrates, a direct link between trade-offs through hormonal/molecular effectors in sexual signals and immunity has not been found so far. Here, we assessed how variation in sexual signals affected parasite infection in two sex pheromone selected lines of the moth Chloridea virescens: an attractive line with a low ratio of 16:Ald/Z11-16:Ald and an unattractive line with a high ratio. When infecting these lines with an apicomplexan parasite, we found that the attractive Low line was significantly more susceptible to the parasite infection than the unattractive High line. Since the ratio difference between these two lines is determined by a delta-11-desturase, we hypothesized that this desaturase may have a dual role, i.e., in the quality of the sexual signal as well as an involvement in immune response, comparable to testosterone in vertebrates. However, when we used CRISPR/cas9 to knockout delta-11-desturase in the attractive Low line, we found that the pheromonal phenotype did change to that of the High line, but the infection susceptibility did not. Notably, when checking the genomic location of delta-11-desaturase in the C. virescens, we found that mucin is adjacent to delta-11-desaturase. When comparing the mucin sequences in both lines, we found four nonsynonymous SNPs in the coding sequence, as well as intronic variation between the two lines. These differences suggest that genetic hitchhiking may explain the variation in susceptibility to parasitic infection.

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