In-vivo Measurements of Tissue Impeditivity by Electrical Impedance Spectroscopy.

The electrical properties of biological tissues differ depending on their structural characteristics. In literature, a lot of study have been carried out with the intent of taking advantage of bioimpedance analysis. Unfortunately, many apparatuses used during these evaluations were not always designed for measurements on living tissues. As a consequence, data could be affected by electrode polarization. In 2016, we presented a new impedance meter, developed for measurements on living tissues. Initially, it was tested only on ex-vivo rabbit's tissues with promising results. As a continuation, this device has been tested on in-vivo samples by placing a needle-probe into 3 tissues (liver, spleen, ovary) of 2 female dogs. Furthermore, was evaluated also the bioimpedance signal of the ovary explanted, comparing it with the in-vivo data. Bioimpedance was analyzed in terms of modulus and phase along a broad spectrum of frequencies (10Hz - 10kHz).Data obtained confirm the possibility of discriminating among the 3 tested tissues, at high frequencies for modulus and at low for phase. Confirmation that values on in-vivo and exvivo tissues are comparable if detected within few minutes after the explant, is also reported. We conclude that this clinical evaluation confirmed, also in-vivo, the good performance of the device previously tested on ex-vivo tissues, and provide more information about the tissue properties and characteristics.

Reflux esophagitis in three cats associated with metaplastic columnar esophageal epithelium.

Gastroesophageal reflux is a relatively common condition in dogs and cats and may lead to secondary reflux esophagitis. A consequence of chronic gastroesophageal reflux that is well described in humans is Barrett's esophagus, which is the replacement of the normal squamous epithelium of the distal esophagus with metaplastic columnar epithelium. Three cats with clinical and endoscopic signs of chronic esophagitis had metaplastic columnar epithelium on biopsy of the distal esophageal mucosa. Suspected underlying causes were cardial incompetence and sliding hiatal hernia. Two cats had complete resolution of the clinical signs after treatment. One cat was euthanized.

Mechanisms, causes, investigation and management of vomiting disorders in cats: a literature review.

Vomiting is a common presenting complaint in feline practice. This article differs from previous reviews in that it is an evidence-based review of the mechanisms, causes, investigation and management of vomiting in the domestic cat. Published evidence was reviewed, and then used to make recommendations for clinical assessment, diagnosis, antiemetic drug treatment, dietary management and monitoring of cats presenting with vomiting. The strength of the evidence on which recommendations are made (and areas where evidence is lacking for cats) has been highlighted throughout.

Grey eosinophils in sighthounds: frequency in 3 breeds and comparison of eosinophil counts determined manually and with 2 hematology analyzers.

BACKGROUND: Grey eosinophils (GE) reported to occur in Greyhounds, and occasionally in other breeds, have clear granules, probably due to abnormal staining properties. OBJECTIVES: The aims of this study were to investigate the frequency of GE in Greyhounds and 2 other sighthound breeds, and to assess the capacity of the ADVIA 120 and Sysmex XT-2000iV hematology analyzers to correctly identify GE. METHODS: Blood samples from 20 Greyhounds, 29 Italian Greyhounds, and 24 Whippets were analyzed using the ADVIA and Sysmex hematology analyzers, and blood smears stained with May-Grünwald Giemsa were evaluated microscopically. The frequency of samples with GE detected on smears was recorded for each breed. Manual and automated eosinophil counts were compared using a Wilcoxon signed-rank test. Agreement between methods was assessed using Passing-Bablok and Bland-Altman plots. RESULTS: GE were detected in all 3 breeds: 9/20 Greyhounds (45.0%), 10/29 Italian Greyhounds (34.5%), and 5/24 Whippets (62.5%) with no significant differences in the frequency of GE among the breeds. In samples containing GE, both analyzers underestimated the percentage of eosinophils and occasionally eosinophils were not detected at all. When a novel "GE gate" was used, the percentage of eosinophils reported by the Sysmex was similar to that obtained by manual counting. CONCLUSIONS: GE are found in the blood of sighthounds other than Greyhounds. Hematology analyzers may underestimate the percentage of GE, probably due to their abnormal physical or chemical features. Underestimation is slight and usually clinically insignificant, but occasionally eosinophils are completely misclassified. Using the Sysmex analyzer, a GE gate can be designed to normalize the eosinophil count.