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1.
Eur Radiol ; 34(1): 355-366, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37528301

RESUMO

OBJECTIVES: To determine whether the texture feature analysis of multi-phase abdominal CT can provide a robust prediction of benign and malignant, histological subtype, pathological stage, nephrectomy risk, pathological grade, and Ki67 index in renal tumor. METHODS: A total of 1051 participants with renal tumor were split into the internal cohort (850 patients from four different hospitals) and the external testing cohort (201 patients from another local hospital). The proposed framework comprised a 3D-kidney and tumor segmentation model by 3D-UNet, a feature extractor for the regions of interest based on radiomics and image dimension reduction, and the six classifiers by XGBoost. A quantitative model interpretation method called SHAP was used to explore the contribution of each feature. RESULTS: The proposed multi-phase abdominal CT model provides robust prediction for benign and malignant, histological subtype, pathological stage, nephrectomy risk, pathological grade, and Ki67 index in the internal validation set, with the AUROC values of 0.88 ± 0.1, 0.90 ± 0.1, 0.91 ± 0.1, 0.89 ± 0.1, 0.84 ± 0.1, and 0.88 ± 0.1, respectively. The external testing set also showed impressive results, with AUROC values of 0.83 ± 0.1, 0.83 ± 0.1, 0.85 ± 0.1, 0.81 ± 0.1, 0.79 ± 0.1, and 0.81 ± 0.1, respectively. The radiomics feature including the first-order statistics, the tumor size-related morphology, and the shape-related tumor features contributed most to the model predictions. CONCLUSIONS: Automatic texture feature analysis of abdominal multi-phase CT provides reliable predictions for multi-tasks, suggesting the potential usage of clinical application. CLINICAL RELEVANCE STATEMENT: The automatic texture feature analysis framework, based on multi-phase abdominal CT, provides robust and reliable predictions for multi-tasks. These valuable insights can serve as a guiding tool for clinical diagnosis and treatment, making medical imaging an essential component in the process. KEY POINTS: • The automatic texture feature analysis framework based on multi-phase abdominal CT can provide more accurate prediction of benign and malignant, histological subtype, pathological stage, nephrectomy risk, pathological grade, and Ki67 index in renal tumor. • The quantitative decomposition of the prediction model was conducted to explore the contribution of the extracted feature. • The study involving 1051 patients from 5 medical centers, along with a heterogeneous external data testing strategy, can be seamlessly transferred to various tasks involving new datasets.


Assuntos
Carcinoma de Células Renais , Neoplasias Renais , Humanos , Carcinoma de Células Renais/patologia , Antígeno Ki-67 , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos , Neoplasias Renais/diagnóstico por imagem , Neoplasias Renais/cirurgia , Neoplasias Renais/patologia
2.
BMC Cardiovasc Disord ; 24(1): 335, 2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38961354

RESUMO

BACKGROUND: The efficacy of optimal medical therapy (OMT) with or without revascularization therapy in patients with stable coronary artery disease (SCAD) remains controversial. We performed a meta-analysis of randomized controlled trials (RCTs) that compared OMT with or without revascularization therapy for SCAD patients. METHODS: Studies were searched in PubMed, EMBASE, and the Cochrane Central Register of Clinical Trials from January 1, 2005, to December 30, 2023. The main efficacy outcome was a composite of all-cause death, myocadiac infarction, revascularization, and cerebrovascular accident. Results were pooled using random effects model and fixed effects model and are presented as odd ratios (ORs) with 95% confidence intervals (CI). RESULTS: Ten studies involving 12,790 participants were included. The arm of OMT with revascularization compared with OMT alone was associated with decreased risks for MACCE (OR 0.55 [95% CI 0.38-0.80], I²=93%, P = 0.002), CV death (OR 0.84 [95% CI 0.73-0.97], I²=36%, P = 0.02), revascularization (OR 0.32 [95% CI 0.20-0.50], I²=92%, P < 0.001), and MI (OR 0.85 [95% CI 0.76-0.96], I²=45%, P = 0.007). While there was no significant difference between OMT with revascularization and OMT alone in the odds of all-cause death (OR 0.94 [95% CI 0.84-1.05], I²=0%, P = 0.30). CONCLUSIONS: The current updated meta-analysis of 10 RCTs shows that in patients with SCAD, OMT with revascularization would reduce the risk for MACCE, cardiovascular death, and MI. However, the invasive strategy does not decrease the risks for all-cause mortality when comparing with OMT alone.


Assuntos
Doença da Artéria Coronariana , Ensaios Clínicos Controlados Aleatórios como Assunto , Humanos , Doença da Artéria Coronariana/mortalidade , Doença da Artéria Coronariana/terapia , Doença da Artéria Coronariana/diagnóstico por imagem , Resultado do Tratamento , Fatores de Risco , Feminino , Masculino , Idoso , Pessoa de Meia-Idade , Fármacos Cardiovasculares/uso terapêutico , Fármacos Cardiovasculares/efeitos adversos , Intervenção Coronária Percutânea/efeitos adversos , Intervenção Coronária Percutânea/mortalidade , Medição de Risco , Revascularização Miocárdica/efeitos adversos , Revascularização Miocárdica/mortalidade , Fatores de Tempo
3.
Sci Total Environ ; 831: 154961, 2022 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-35367544

RESUMO

Air pollution is a major concern of the new civilized world due to its adverse impact on human health and environment. As typical air pollutants, nitrogen oxide (NOX) and sulfur dioxide (SO2) not only pollute the atmosphere by forming acid rain and particulate matter, but are also harmful to the human respiratory system. Significant emissions of NOX and SO2 in the production phases make the textile industry under enormous environmental pressure. Chemical footprint (ChF) is an effective method for transforming the potential environmental risks of pollutant emissions into an intuitive form of toxicity. In this study, we present a ChF assessment method for NOX and SO2 emissions from textiles production. For this purpose, we adopt the USEtox model and calculate the relevant characterization factors (CFs) by considering the physicochemical properties and toxicity of NOX and SO2. The textile industry in Zhejiang Province, China, is chosen as a case study to demonstrate the feasibility of this proposed ChF assessment methodology. Results indicate that ChF caused by NOX emission in Zhejiang's textile industry is approximately eight times larger than that caused by SO2 emission. The four sub-sectors of Zhejiang's textile industry (textile manufacturing sector; textile wearing apparel, footware, and caps manufacturing sector; leather, fur, feather and related products manufacturing sector; chemical fibers manufacturing sector) also have similar proportional distributions of ChFs. Besides, the textile manufacturing sector has the largest ChF, accounting for 73% of the total ChF caused by NOX and SO2 emissions.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China , Meio Ambiente , Monitoramento Ambiental/métodos , Humanos , Material Particulado/análise , Dióxido de Enxofre , Têxteis
4.
J Pediatr ; 157(4): 623-9, 629.e1, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20553844

RESUMO

OBJECTIVE: To test the hypothesis that predisposition to childhood herpes simplex virus (HSV) type 1 encephalitis (HSE) may be determined in part by human genetic factors. STUDY DESIGN: A genetic epidemiologic survey of childhood HSE (onset at age 3 months to 15 years) over a 20-year period (1985-2004) was conducted throughout France (comprising 29 university hospital neuropediatric centers). A total of 85 children fulfilled the diagnostic criteria for inclusion. Family and personal histories were obtained by face-to-face interview for 51 patients. RESULTS: No familial cases of HSE were identified in our survey; however, a high proportion (20%) of the children interviewed had a relevant family history: parental consanguinity (12% of patients), early-onset herpetic keratitis in a first-degree relative (6%), or both (2%). The narrow window of high susceptibility to HSE before age 3 years (62% of patients) further indicates that predisposition to HSE is tightly age-dependent. CONCLUSIONS: This survey suggests that childhood HSE, although sporadic, may result from Mendelian predisposition (from autosomal recessive susceptibility in particular), at least in some children. There likely is incomplete penetrance, however, which may reflect, at least in part, the impact of age at the time of HSV-1 infection.


Assuntos
Encefalite por Herpes Simples/genética , Encefalite por Herpes Simples/virologia , Variação Genética , Receptor 3 Toll-Like/genética , Aciclovir/uso terapêutico , Adolescente , Fatores Etários , Idade de Início , Antivirais/uso terapêutico , Criança , Pré-Escolar , Encefalite por Herpes Simples/tratamento farmacológico , Feminino , Predisposição Genética para Doença , Variação Genética/genética , Humanos , Lactente , Masculino , Fatores de Risco , Simplexvirus , Adulto Jovem
5.
J Exp Med ; 209(9): 1567-82, 2012 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-22851595

RESUMO

Childhood herpes simplex virus-1 (HSV-1) encephalitis (HSE) may result from single-gene inborn errors of TLR3 immunity. TLR3-dependent induction of IFN-α/ß or IFN-λ is crucial for protective immunity against primary HSV-1 infection in the central nervous system (CNS). We describe here two unrelated children with HSE carrying different heterozygous mutations (D50A and G159A) in TBK1, the gene encoding TANK-binding kinase 1, a kinase at the crossroads of multiple IFN-inducing signaling pathways. Both mutant TBK1 alleles are loss-of-function but through different mechanisms: protein instability (D50A) or a loss of kinase activity (G159A). Both are also associated with an autosomal-dominant (AD) trait but by different mechanisms: haplotype insufficiency (D50A) or negative dominance (G159A). A defect in polyinosinic-polycytidylic acid-induced TLR3 responses can be detected in fibroblasts heterozygous for G159A but not for D50A TBK1. Nevertheless, viral replication and cell death rates caused by two TLR3-dependent viruses (HSV-1 and vesicular stomatitis virus) were high in fibroblasts from both patients, and particularly so in G159A TBK1 fibroblasts. These phenotypes were rescued equally well by IFN-α2b. Moreover, the IFN responses to the TLR3-independent agonists and viruses tested were maintained in both patients' peripheral blood mononuclear cells and fibroblasts. The narrow, partial cellular phenotype thus accounts for the clinical phenotype of these patients being limited to HSE. These data identify AD partial TBK1 deficiency as a new genetic etiology of childhood HSE, indicating that TBK1 is essential for the TLR3- and IFN-dependent control of HSV-1 in the CNS.


Assuntos
Encefalite por Herpes Simples/genética , Encefalite por Herpes Simples/imunologia , Mutação , Proteínas Serina-Treonina Quinases/genética , Receptor 3 Toll-Like/imunologia , Animais , Morte Celular/imunologia , Células Cultivadas , Criança , Feminino , Fibroblastos/efeitos dos fármacos , Fibroblastos/imunologia , Fibroblastos/virologia , Genes Dominantes , Herpesvirus Humano 1/patogenicidade , Humanos , Interferon beta/imunologia , Masculino , Camundongos , Poli I-C/farmacologia , Proteínas Serina-Treonina Quinases/imunologia , Vesiculovirus/patogenicidade
6.
J Clin Invest ; 121(12): 4889-902, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22105173

RESUMO

Herpes simplex encephalitis (HSE) is the most common sporadic viral encephalitis of childhood. Autosomal recessive (AR) UNC-93B and TLR3 deficiencies and autosomal dominant (AD) TLR3 and TRAF3 deficiencies underlie HSE in some children. We report here unrelated HSE children with AR or AD TRIF deficiency. The AR form of the disease was found to be due to a homozygous nonsense mutation that resulted in a complete absence of the TRIF protein. Both the TLR3- and the TRIF-dependent TLR4 signaling pathways were abolished. The AD form of disease was found to be due to a heterozygous missense mutation, resulting in a dysfunctional protein. In this form of the disease, the TLR3 signaling pathway was impaired, whereas the TRIF-dependent TLR4 pathway was unaffected. Both patients, however, showed reduced capacity to respond to stimulation of the DExD/H-box helicases pathway. To date, the TRIF-deficient patients with HSE described herein have suffered from no other infections. Moreover, as observed in patients with other genetic etiologies of HSE, clinical penetrance was found to be incomplete, as some HSV-1-infected TRIF-deficient relatives have not developed HSE. Our results provide what we believe to be the first description of human TRIF deficiency and a new genetic etiology for HSE. They suggest that the TRIF-dependent TLR4 and DExD/H-box helicase pathways are largely redundant in host defense. They further demonstrate the importance of TRIF for the TLR3-dependent production of antiviral IFNs in the CNS during primary infection with HSV-1 in childhood.


Assuntos
Proteínas Adaptadoras de Transporte Vesicular/deficiência , Encefalite por Herpes Simples/genética , Herpesvirus Humano 1 , Proteínas Adaptadoras de Transporte Vesicular/genética , Proteínas Adaptadoras de Transporte Vesicular/fisiologia , Sequência de Aminoácidos , Pré-Escolar , Códon sem Sentido , Consanguinidade , RNA Helicases DEAD-box/fisiologia , Feminino , Genes Dominantes , Genes Recessivos , Heterogeneidade Genética , Predisposição Genética para Doença , Estudo de Associação Genômica Ampla , Genótipo , Humanos , Lactente , Interferon-alfa/biossíntese , Interferon-alfa/genética , Masculino , Dados de Sequência Molecular , Mutação de Sentido Incorreto , Linhagem , Arábia Saudita , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Transdução de Sinais/fisiologia , Receptor 3 Toll-Like/fisiologia , Receptor 4 Toll-Like/fisiologia
7.
J Exp Med ; 208(10): 2083-98, 2011 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-21911422

RESUMO

Autosomal dominant TLR3 deficiency has been identified as a genetic etiology of childhood herpes simplex virus 1 (HSV-1) encephalitis (HSE). This defect is partial, as it results in impaired, but not abolished induction of IFN-ß and -λ in fibroblasts in response to TLR3 stimulation. The apparently normal resistance of these patients to other infections, viral illnesses in particular, may thus result from residual TLR3 responses. We report here an autosomal recessive form of complete TLR3 deficiency in a young man who developed HSE in childhood but remained normally resistant to other infections. This patient is compound heterozygous for two loss-of-function TLR3 alleles, resulting in an absence of response to TLR3 activation by polyinosinic-polycytidylic acid (poly(I:C)) and related agonists in his fibroblasts. Moreover, upon infection of the patient's fibroblasts with HSV-1, the impairment of IFN-ß and -λ production resulted in high levels of viral replication and cell death. In contrast, the patient's peripheral blood mononuclear cells responded normally to poly(I:C) and to all viruses tested, including HSV-1. Consistently, various TLR3-deficient leukocytes from the patient, including CD14(+) and/or CD16(+) monocytes, plasmacytoid dendritic cells, and in vitro derived monocyte-derived macrophages, responded normally to both poly(I:C) and HSV-1, with the induction of antiviral IFN production. These findings identify a new genetic etiology for childhood HSE, indicating that TLR3-mediated immunity is essential for protective immunity to HSV-1 in the central nervous system (CNS) during primary infection in childhood, in at least some patients. They also indicate that human TLR3 is largely redundant for responses to double-stranded RNA and HSV-1 in various leukocytes, probably accounting for the redundancy of TLR3 for host defense against viruses, including HSV-1, outside the CNS.


Assuntos
Encefalite por Herpes Simples/imunologia , Imunidade/imunologia , Simplexvirus/imunologia , Receptor 3 Toll-Like/deficiência , Células Cultivadas , Análise Mutacional de DNA , Encefalite por Herpes Simples/genética , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/fisiologia , Estudo de Associação Genômica Ampla , Humanos , Leucócitos Mononucleares/citologia , Leucócitos Mononucleares/imunologia , Masculino , Mutação , Fator 88 de Diferenciação Mieloide/genética , Fator 88 de Diferenciação Mieloide/imunologia , Linhagem , Poli I-C/imunologia , Poli I-C/farmacologia , Simplexvirus/genética , Receptor 3 Toll-Like/genética , Adulto Jovem
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