RESUMO
Most cases of oral squamous cell carcinoma (OSCC) develop from visible oral potentially malignant disorders (OPMDs). The latter exhibit heterogeneous subtypes with different transformation potentials, complicating the early detection of OSCC during routine visual oral cancer screenings. To develop clinically applicable biomarkers, we collected saliva samples from 96 healthy controls, 103 low-risk OPMDs, 130 high-risk OPMDs, and 131 OSCC subjects. These individuals were enrolled in Taiwan's Oral Cancer Screening Program. We identified 302 protein biomarkers reported in the literature and/or through in-house studies and prioritized 49 proteins for quantification in the saliva samples using multiple reaction monitoring-MS. Twenty-eight proteins were successfully quantified with high confidence. The quantification data from non-OSCC subjects (healthy controls + low-risk OPMDs) and OSCC subjects in the training set were subjected to classification and regression tree analyses, through which we generated a four-protein panel consisting of MMP1, KNG1, ANXA2, and HSPA5. A risk-score scheme was established, and the panel showed high sensitivity (87.5%) and specificity (80.5%) in the test set to distinguish OSCC samples from non-OSCC samples. The risk score >0.4 detected 84% (42/50) of the stage I OSCCs and a significant portion (42%) of the high-risk OPMDs. Moreover, among 88 high-risk OPMD patients with available follow-up results, 18 developed OSCC within 5 y; of them, 77.8% (14/18) had risk scores >0.4. Our four-protein panel may therefore offer a clinically effective tool for detecting OSCC and monitoring high-risk OPMDs through a readily available biofluid.
Assuntos
Biomarcadores Tumorais/metabolismo , Carcinoma de Células Escamosas/metabolismo , Neoplasias Bucais/metabolismo , Proteínas e Peptídeos Salivares/metabolismo , Carcinoma de Células Escamosas/patologia , Cromatografia Líquida , Demografia , Detecção Precoce de Câncer , Chaperona BiP do Retículo Endoplasmático , Feminino , Seguimentos , Humanos , Masculino , Espectrometria de Massas , Pessoa de Meia-Idade , Neoplasias Bucais/patologia , Estadiamento de Neoplasias , Fatores de Risco , Saliva/metabolismo , TaiwanAssuntos
Neoplasias Bucais , Saliva , Biomarcadores , Biomarcadores Tumorais , Carcinoma de Células Escamosas , HumanosRESUMO
The discovery of genes that control cell division in yeast, and their relation to cancer, is reviewed.
Assuntos
Proteínas de Ciclo Celular/genética , Variação Genética , Neoplasias/genética , Neoplasias/patologia , Leveduras/fisiologia , Proteínas de Ciclo Celular/história , Proteínas de Ciclo Celular/metabolismo , Divisão Celular/genética , Dano ao DNA/genética , História do Século XX , História do Século XXI , Neoplasias/história , Neoplasias/metabolismo , Prêmio Nobel , Estados Unidos , Leveduras/genéticaRESUMO
The discovery of genes that control cell division in yeast, and their relation to cancer, is reviewed.