A Miniature Bio-Photonics Companion Diagnostics Platform for Reliable Cancer Treatment Monitoring in Blood Fluids.

In this paper, we present the development of a photonic biosensor device for cancer treatment monitoring as a complementary diagnostics tool. The proposed device combines multidisciplinary concepts from the photonic, nano-biochemical, micro-fluidic and reader/packaging platforms aiming to overcome limitations related to detection reliability, sensitivity, specificity, compactness and cost issues. The photonic sensor is based on an array of six asymmetric Mach Zender Interferometer (aMZI) waveguides on silicon nitride substrates and the sensing is performed by measuring the phase shift of the output signal, caused by the binding of the analyte on the functionalized aMZI surface. According to the morphological design of the waveguides, an improved sensitivity is achieved in comparison to the current technologies (<5000 nm/RIU). This platform is combined with a novel biofunctionalization methodology that involves material-selective surface chemistries and the high-resolution laser printing of biomaterials resulting in the development of an integrated photonics biosensor device that employs disposable microfluidics cartridges. The device is tested with cancer patient blood serum samples. The detection of periostin (POSTN) and transforming growth factor beta-induced protein (TGFBI), two circulating biomarkers overexpressed by cancer stem cells, is achieved in cancer patient serum with the use of the device.

High performance refractive index sensor based on low Q-factor ring resonators and FFT processing of wavelength scanning data.

We extend our previous simulation study and we present experimental results regarding our Fast Fourier Transform method for the calculation of the resonance shifts in biosensors based on micro-ring resonators (MRRs). For the simulation study, we use a system model with a tunable laser at 850 nm, an MRR with 1.5∙104 quality factor, and a detection system with 50 dB maximum signal-to-noise ratio, and investigate the impact on the system performance of factors like the number of the resonance peaks inside the scanning window, the wavelength dependence of the laser power, and the asymmetry of the transfer functions of the MRRs. We find that the performance is improved by a factor of 2 when we go from single- to four-peak transfer functions, and that the impact of the wavelength dependence of the laser power is very low. We also find that the presence of asymmetries can lead to strong discontinuities of the transfer functions at the edges of the scanning window and can significantly increase the measurement errors, making necessary the use of techniques for their elimination. Using these conclusions, we build a system with sensing MRRs on TriPleX platform, and we experimentally validate our method using sucrose solutions with different concentrations. Involving techniques in order to exclude the noise originating from the microfluidic system, we achieve a wavelength resolution close to 0.08 pm, when the system operates with 0.5 pm scanning step. In combination with the sensitivity of the MRRs, which is measured to be equal to 93.7 nm/RIU, this wavelength resolution indicates the possibility for a limit of detection close to 8.5·10-7 RIU, which represents to the best of our knowledge a record performance for this type of optical sensors and this level of scanning steps.

Two-octave spanning supercontinuum generation in stoichiometric silicon nitride waveguides pumped at telecom wavelengths.

We demonstrate supercontinuum generation in stoichiometric silicon nitride (Si3N4 in SiO2) integrated optical waveguides, pumped at telecommunication wavelengths. The pump laser is a mode-locked erbium fiber laser at a wavelength of 1.56 µm with a pulse duration of 120 fs. With a waveguide-internal pulse energy of 1.4 nJ and a waveguide with 1.0 µm × 0.9 µm cross section, designed for anomalous dispersion across the 1500 nm telecommunication range, the output spectrum extends from the visible, at around 526 nm, up to the mid-infrared, at least to 2.6 µm, the instrumental limit of our detection. This output spans more than 2.2 octaves (454 THz at the -30 dB level). The measured output spectra agree well with theoretical modeling based on the generalized nonlinear Schrödinger equation. The infrared part of the supercontinuum spectra shifts progressively towards the mid-infrared, well beyond 2.6 µm, by increasing the width of the waveguides.

New set of design rules for resonant refractive index sensors enabled by FFT based processing of the measurement data.

It is still a common belief that ultra-high quality-factors (Q-factors) are a prerequisite in optical resonant cavities for high refractive index resolution and low detection limit in biosensing applications. In combination with the ultra-short steps that are necessary when the measurement of the resonance shift relies on the wavelength scanning of a laser source and conventional methods for data processing, the high Q-factor requirement makes these biosensors extremely impractical. In this work we analyze an alternative processing method based on the fast-Fourier transform, and show through Monte-Carlo simulations that improvement by 2-3 orders of magnitude can be achieved in the resolution and the detection limit of the system in the presence of amplitude and spectral noise. More significantly, this improvement is maximum for low Q-factors around 104 and is present also for high intra-cavity losses and large scanning steps making the designs compatible with the low-cost aspect of lab-on-a-chip technology. Using a micro-ring resonator as model cavity and a system design with low Q-factor (104), low amplitude transmission (0.85) and relatively large scanning step (0.25 pm), we show that resolution close to 0.01 pm and detection limit close to 10-7 RIU can be achieved improving the sensing performance by more than 2 orders of magnitude compared to the performance of systems relying on a simple peak search processing method. The improvement in the limit of detection is present even when the simple method is combined with ultra-high Q-factors and ultra-short scanning steps due to the trade-off between the system resolution and sensitivity. Early experimental results are in agreement with the trends of the numerical studies.

Stress-optic modulator in TriPleX platform using a ezoelectric lead zirconate titanate (PZT) thin film.

We will demonstrate a stress-optic phase modulator in the passive SiN-based TriPleX platform using a layer of piezoelectric material. Regarding the stress-optic effect, the piezoelectric layer deposited on top of an optical waveguide is employed to control the phase of propagating light in the structure by applying an electrical field across the layer. In this work, it is demonstrated that the stress-optic effect lowers the power consumption by a factor of one million for quasi-DC operation and increases the modulation speed by three orders of magnitude, compared to currently used thermo-optic modulation in the TriPleX platform.

High confinement, high yield Si(3)N(4) waveguides for nonlinear optical applications.

In this paper we present a novel fabrication technique for silicon nitride (Si(3)N(4)) waveguides with a thickness of up to 900 nm, which are suitable for nonlinear optical applications. The fabrication method is based on etching trenches in thermally oxidized silicon and filling the trenches with Si(3)N(4). Using this technique no stress-induced cracks in the Si(3)N(4) layer were observed resulting in a high yield of devices on the wafer. The propagation losses of the obtained waveguides were measured to be as low as 0.4 dB/cm at a wavelength of around 1550 nm.

On-chip visible-to-infrared supercontinuum generation with more than 495 THz spectral bandwidth.

We report ultra-broadband supercontinuum generation in high-confinement Si3N4 integrated optical waveguides. The spectrum extends through the visible (from 470 nm) to the infrared spectral range (2130 nm) comprising a spectral bandwidth wider than 495 THz, which is the widest supercontinuum spectrum generated on a chip.

On-chip optical trapping and Raman spectroscopy using a TripleX dual-waveguide trap.

We present a new approach to the dual-beam geometry for on-chip optical trapping and Raman spectroscopy, using waveguides microfabricated in TripleX technology. Such waveguides are box shaped and consist of SiO2 and Si3N4, so as to provide a low index contrast with respect to the SiO2 claddings and low loss, while retaining the advantages of Si3N4. The waveguides enable both the trapping and Raman functionality with the same dual beams. Polystyrene beads of 1 µm diameter can be easily trapped with the device. In the axial direction discrete trapping positions occur, owing to the intensity pattern of the interfering beams. Trapping events are interpreted on the basis of simulated optical fields and calculated optical forces. The average transverse trap stiffness is 0.8 pN/nm/W, indicating that a strong trap is formed by the beams emitted by the waveguides. Finally, we measure Raman spectra of trapped beads for short integration times (down to 0.25 s), which is very promising for Raman spectroscopy of microbiological cells.

On-chip microwave photonic beamformer circuits operating with phase modulation and direct detection.

We propose and experimentally demonstrate the working principles of two novel microwave photonic (MWP) beamformer circuits operating with phase modulation (PM) and direct detection (DD). The proposed circuits incorporate two major signal processing functionalities, namely a broadband beamforming network employing ring resonator-based delay lines and an optical sideband manipulator that renders the circuit outputs equivalent to those of intensity-modulated MWP beamformers. These functionalities allow the system to employ low-circuit-complexity modulators and detectors, which brings significant benefits on the system construction cost and operation stability. The functionalities of the proposed MWP beamformer circuits were verified in experimental demonstrations performed on two sample circuits realized in Si(3)N(4)/SiO(2) waveguide technology. The measurements exhibit a 2 × 1 beamforming effect for an instantaneous RF transmission band of 3‒7 GHz, which is, to our best knowledge, the first verification of on-chip MWP beamformer circuits operating with PM and DD.

Ring resonator-based on-chip modulation transformer for high-performance phase-modulated microwave photonic links.

In this paper, we propose and experimentally demonstrate a novel wideband on-chip photonic modulation transformer for phase-modulated microwave photonic links. The proposed device is able to transform phase-modulated optical signals into intensity-modulated versions (or vice versa) with nearly zero conversion of laser phase noise to intensity noise. It is constructed using waveguide-based ring resonators, which features simple architecture, stable operation, and easy reconfigurability. Beyond the stand-alone functionality, the proposed device can also be integrated with other functional building blocks of photonic integrated circuits (PICs) to create on-chip complex microwave photonic signal processors. As an application example, a PIC consisting of two such modulation transformers and a notch filter has been designed and realized in TriPleX(TM) waveguide technology. The realized device uses a 2 × 2 splitting circuit and 3 ring resonators with a free spectral range of 25 GHz, which are all equipped with continuous tuning elements. The device can perform phase-to-intensity modulation transform and carrier suppression simultaneously, which enables high-performance phase-modulated microwave photonics links (PM-MPLs). Associated with the bias-free and low-complexity advantages of the phase modulators, a single-fiber-span PM-MPL with a RF bandwidth of 12 GHz (3 dB-suppression band 6 to 18 GHz) has been demonstrated comprising the proposed PIC, where the achieved spurious-free dynamic range performance is comparable to that of Class-AB MPLs using low-biased Mach-Zehnder modulators.

Novel wideband microwave polarization network using a fully-reconfigurable photonic waveguide interleaver with a two-ring resonator-assisted asymmetric Mach-Zehnder structure.

We propose and demonstrate a novel wideband microwave photonic polarization network for dual linear-polarized antennas. The polarization network is based on a waveguide-implemented fully-reconfigurable optical interleaver using a two-ring resonator-assisted asymmetric Mach-Zehnder structure. For microwave photonic signal processing, this structure is able to serve as a wideband 2 × 2 RF coupler with reconfigurable complex coefficients, and therefore can be used as a polarization network for wideband antennas. Such a device can equip the antennas with not only the polarization rotation capability for linear-polarization signals but also the capability to operate with and tune between two opposite circular polarizations. Operating together with a particular modulation scheme, the device is also able to serve for simultaneous feeding of dual-polarization signals. These photonic-implemented RF functionalities can be applied to wideband antenna systems to perform agile polarization manipulations and tracking operations. An example of such a interleaver has been realized in TriPleX waveguide technology, which was designed with a free spectral range of 20 GHz and a mask footprint of smaller than 1 × 1 cm. Using the realized device, the reconfigurable complex coefficients of the polarization network were demonstrated with a continuous bandwidth from 2 to 8 GHz and an in-band phase ripple of smaller than 5 degree. The waveguide structure of the device allows it to be further integrated with other functional building blocks of a photonic integrated circuit to realize on-chip, complex microwave photonic processors. Of particular interest, it can be included in an optical beamformer for phased array antennas, so that simultaneous wideband beam and polarization trackings can be achieved photonically. To our knowledge, this is the first-time on-chip demonstration of an integrated microwave photonic polarization network for dual linear-polarized antennas.

Si3N4 ring resonator-based microwave photonic notch filter with an ultrahigh peak rejection.

We report a simple technique in microwave photonic (MWP) signal processing that allows the use of an optical filter with a shallow notch to exhibit a microwave notch filter with anomalously high rejection level. We implement this technique using a low-loss, tunable Si3N4 optical ring resonator as the optical filter, and achieved an MWP notch filter with an ultra-high peak rejection > 60 dB, a tunable high resolution bandwidth of 247-840 MHz, and notch frequency tuning of 2-8 GHz. To our knowledge, this is a record combined peak rejection and resolution for an integrated MWP filter.

Silicon nitride microwave photonic circuits.

We present an overview of several microwave photonic processing functionalities based on combinations of Mach-Zehnder and ring resonator filters using the high index contrast silicon nitride (TriPleX™) waveguide technology. All functionalities are built using the same basic building blocks, namely straight waveguides, phase tuning elements and directional couplers. We recall previously shown measurements on high spurious free dynamic range microwave photonic (MWP) link, ultra-wideband pulse generation, instantaneous frequency measurements, Hilbert transformers, microwave polarization networks and demonstrate new measurements and functionalities on a 16 channel optical beamforming network and modulation format transformer as well as an outlook on future microwave photonic platform integration, which will lead to a significantly reduced footprint and thereby enables the path to commercially viable MWP systems.

Novel microwave photonic fractional Hilbert transformer using a ring resonator-based optical all-pass filter.

We propose and demonstrate a novel wideband microwave photonic fractional Hilbert transformer implemented using a ring resonator-based optical all-pass filter. The full programmability of the ring resonator allows variable and arbitrary fractional order of the Hilbert transformer. The performance analysis in both frequency and time domain validates that the proposed implementation provides a good approximation to an ideal fractional Hilbert transformer. This is also experimentally verified by an electrical S21 response characterization performed on a waveguide realization of a ring resonator. The waveguide-based structure allows the proposed Hilbert transformer to be integrated together with other building blocks on a photonic integrated circuit to create various system-level functionalities for on-chip microwave photonic signal processors. As an example, a circuit consisting of a splitter and a ring resonator has been realized which can perform on-chip phase control of microwave signals generated by means of optical heterodyning, and simultaneous generation of in-phase and quadrature microwave signals for a wide frequency range. For these functionalities, this simple and on-chip solution is considered to be practical, particularly when operating together with a dual-frequency laser. To our best knowledge, this is the first-time on-chip demonstration where ring resonators are employed to perform phase control functionalities for optical generation of microwave signals by means of optical heterodyning.

System integration and radiation pattern measurements of a phased array antenna employing an integrated photonic beamformer for radio astronomy applications.

In this paper we describe the system integration and the experimental demonstration of a photonically beamformed four-element receiving array antenna for radio astronomy applications. To our knowledge, the work described here is the first demonstration of the squint-free, continuously tunable beamsteering capability offered by an integrated photonic beamformer based on optical ring resonator true-time-delay units, with measured radiation patterns. The integrated beamformer is realized in a low loss, complementary metal-oxide-semiconductor (CMOS) compatible optical waveguide technology. The measurements show a wideband, continuous beamsteering operation over a steering angle of 23.5 degrees and an instantaneous bandwidth of 500 MHz limited only by the measurement setup.

On-chip CMOS compatible reconfigurable optical delay line with separate carrier tuning for microwave photonic signal processing.

We report, for the first time, an integrated photonic signal processor consisting of a reconfigurable optical delay line (ODL) with a separate carrier tuning (SCT) unit and an optical sideband filter on a single CMOS compatible photonic chip. The processing functionalities are carried out with optical ring resonators as building blocks. We show that the integrated approach together with the use of SCT technique allows the implementation of a wideband, fully-tunable ODL with reduced complexity. To highlight the functionalities of the processor, we demonstrate a reconfigurable microwave photonic filter where the ODL has been configured in a bandwidth over 1 GHz.

Planar waveguides with less than 0.1 dB/m propagation loss fabricated with wafer bonding.

We demonstrate a wafer-bonded silica-on-silicon planar waveguide platform with record low total propagation loss of (0.045 ± 0.04) dB/m near the free space wavelength of 1580 nm. Using coherent optical frequency domain reflectometry, we characterize the group index, fiber-to-chip coupling loss, critical bend radius, and propagation loss of these waveguides.

Ultra-low-loss high-aspect-ratio Si3N4 waveguides.

We characterize an approach to make ultra-low-loss waveguides using stable and reproducible stoichiometric Si3N4 deposited with low-pressure chemical vapor deposition. Using a high-aspect-ratio core geometry, record low losses of 8-9 dB/m for a 0.5 mm bend radius down to 3 dB/m for a 2 mm bend radius are measured with ring resonator and optical frequency domain reflectometry techniques. From a waveguide loss model that agrees well with experimental results, we project that 0.1 dB/m total propagation loss is achievable at a 7 mm bend radius with this approach.

AFM1 Detection in Milk by Fab' Functionalized Si3N4 Asymmetric Mach-Zehnder Interferometric Biosensors.

Aflatoxins (AF) are naturally occurring mycotoxins, produced by many species of Aspergillus. Among aflatoxins, Aflatoxin M1 (AFM1) is one of the most frequent and dangerous for human health. The acceptable maximum level of AFM1 in milk according to EU regulation is 50 ppt, equivalent to 152 pM, and 25 ppt, equivalent to 76 pM, for adults and infants, respectively. Here, we study a photonic biosensor based on Si 3 N 4 asymmetric Mach-Zehnder Interferometers (aMZI) functionalized with Fab' for AFM1 detection in milk samples (eluates). The minimum concentration of AFM1 detected by our aMZI sensors is 48 pM (16.8 pg/mL) in purified and concentrated milk samples. Moreover, the real-time detection of the ligand-analyte binding enables the study of the kinetics of the reaction. We measured the kinetic rate constants of the Fab'-AFM1 interaction.

TriPleX™ waveguide-based fluorescence biosensor for multichannel environmental contaminants detection.

In order to realize the multi-analyte assays for environmental contaminants, an optical biosensor utilizing laser-induced fluorescence-based detection via the binding of biomolecules to the surface of an integrated TriPleX™ waveguide chip on a glass substrate (fused silica, FS) is described. As far as we know, this is the first demonstration of using the TriPleX™ technology to fabricate the waveguide chip on a FS substrate. The sensor consists of 32 individually addressable sensor patches, which were formed on the chip surface by exploiting 3 Y-junction splitters, creating four equal rows of eight evanescently excited windows in parallel. The basic low-loss SiO2/Si3N4 TriPleX™ waveguide configuration in combination with on-chip spotsize convertors allows for both high fiber-to-chip coupling efficiency and enables at the same time individually optimized high chip surface intensity and low patch-to-patch deviation. Moreover, the complementary metal-oxide-semiconductor compatible fabrication of waveguide chip allows for its mass production at low cost. By taking MC-LR, 2,4-D, atrazine and BPA as the model analytes, the as-proposed waveguide based biosensor was proven sensitive with the detection limits of 0.22 µg/L for MC-LR, 1.18 µg/L for 2, 4-D, 0.2 µg/L for atrazine and 0.06 µg/L for BPA. Recoveries of the biosensor towards simultaneous detection of MC-LR, 2, 4-D, atrazine and BPA in spiked real water samples varied from 84% to 120%, indicating the satisfactory accuracy of the established technology.