Should anti-tuberculosis therapy be prescribed in erythema induratum? A case-controlled and incidence correlation study between 2001 to 2020 in Taiwan.

BACKGROUND: Erythema induratum (EI) is a tuberculid associated with Mycobacterium tuberculosis (TB) infection. TB has been identified in Taiwan with a high percentage of EI using PCR. However, this pathogen has rarely been detected recently. OBJECTIVE: To explore the association between EI, annual incidence of tuberculosis in Taiwan, and treatment outcomes over the past two decades. METHODS: Patients diagnosed with EI between 2001 and 2020 were enrolled based on histopathology, tissue culture, and TB-PCR tests. Other cases of panniculitis with TB-PCR results were used as controls. Their clinical information was obtained. The results were correlated with the annual incidence of tuberculosis and compared between groups. RESULTS: Fifty-five specimens from EI patients fulfilled the inclusion criteria; three (3/55, 5%) had positive TB PCR. One patient diagnosed with erythema nodosum in the control group had positive TB PCR (1/27, 4%). There was no significant relationship between TB and EI (odds ratio = 1.5, 95% confidence interval = -0.964 to 3.964, p = 1). Correlation between the incidence of TB and number of EI cases was not significant (r = -0.185, p = 0.447). Only four patients received anti-tuberculosis therapy. They all showed clinical improvement without recurrence. One patient with PCR-positive EI was not treated with anti-tuberculosis therapy; however, the skin lesion improved 3 months later. No other patients in the EI group were diagnosed with TB infection in the follow up of 508 person-years. CONCLUSIONS: Most EI in Taiwan are nodular vasculitis and not tuberculids owing to well-controlled tuberculosis. This condition can be alleviated without anti-tuberculosis therapy.

A Three-Dimensional Morphological Assessment for Evaluating Pre- and Postsurgery in Cervical Laminoplasty.

OBJECTIVE: The efficacy of medical treatments and the changes in radiologic imaging before and after treatment have consistently remained pivotal factors. This is particularly critical for surgical procedures, where precise evaluation of disparities pre and postsurgery or the accuracy of implantation is paramount. Based on three-dimensional morphological interests, we provide an automatic quantification evaluation method that delivers an evident base for assessing the outcomes of a widely employed surgical technique, cervical laminoplasty. METHODS: The sample study included patients who underwent cervical laminoplasty for cervical spondylotic myelopathy/ossification of the longitudinal ligament. We present a superimposition method that facilitates a unique and precise assessment between pre and postsurgery. The degree of expansion was evaluated by the canal volume increase and canal expansion rate after surgery. RESULTS: There were 31 patients with 112 vertebral segments measured. The target cervical's pre and postoperative canal areas were 122.63 ± 30.34 and 196.50 ± 37.10 mm2, respectively (P < 0.001). The average cervical canal expansion rate was 64.42%. The expansion effect of C5 cervical laminoplasty was the maximum (71.01%), and the canal volume of other segments expanded by approximately 60%. The functional outcomes demonstrated significant improvements in symptoms. CONCLUSIONS: The quantification evaluation method can be utilized for any morphology changes before and after laminoplasty, as it does not lead to errors or variations from different inspection machines or human factors. The automatic method delivers an evident base for assessing the outcomes of a widely employed surgical technique.

A Symmetry-Based Superposition Method for Planning and Surgical Outcome Assessment.

Computer-aided surgical planning has been widely used to increase the safety and predictability of surgery. The validation of the target of surgical planning to surgical outcomes on a patient-specific model is an important issue. The aim of this research was to develop a robust superposition method to assess the deviation of planning and outcome by using the symmetrical characteristic of the affected target. The optimal symmetry plane (OSP) of an object is usually used to evaluate the degree of symmetry of an object. We proposed a refined OSP-based contouring method to transfer a complex three-dimensional superposition operation into two dimensions. We compared the typical iterative closest point (ICP) algorithm with the refined OSP-based contouring method and examined the differences between them. The results using the OSP-based method were much better than the traditional method. As for processing time, the OSP-based contouring method was 11 times faster than the ICP method overall. The proposed method was not affected by the metallic artifacts from medical imaging or geometric changes due to surgical intervention. This technique can be applied for post-operative assessment, such as quantifying the differences between surgical targets and outcomes as well as performing long-term medical follow-up.

An Automatic Voxel-Based Method for Optimal Symmetry Plane Generation for the Maxillofacial Region in Severe Asymmetry Cases.

Symmetry is representative of aesthetics and health in all kinds of vertebrates, especially the human face. Therefore, to automatically locate the appropriate symmetry plane is crucial. The aim of this study was to develop an automatic and reliable method to determine the symmetry plane of the maxillofacial region. We compared the proposed method of determining the symmetry plane by assessing landmark-based and surface-based methods by way of quantitative symmetry assessments. Statistical analysis was applied to evaluate whether significant difference existed among these three kinds of symmetry planes. Twenty cases who had a diagnosis of severe facial asymmetry were evaluated retrospectively. The results showed that searching for the symmetry plane using a voxel-based method, named the optimal symmetry plane (OSP), achieved the most representative symmetry according to the outcomes of the trials. The OSP was significantly more symmetrical than the other two planes, as determined by other methods. The paired-voxel computation method proposed in this research is a robust and reliable method for identifying the unique symmetry plane for patients with severe facial asymmetry. Symmetry is of crucial significance for all kinds of vertebrates, including its clinical implications for surgical planning in orthognathic surgery.

Lamotrigine attenuates the motivation to self-administer ketamine and prevents cue- and prime-induced reinstatement of ketamine-seeking behavior in rats.

BACKGROUND: Lamotrigine is an anticonvulsant drug used in the treatment of epilepsy and bipolar disorder. A case report has demonstrated that a ketamine addict experienced a significant reduction in craving and ketamine use after taking lamotrigine. The present study determined whether lamotrigine can reduce the motivation for ketamine and prevent the relapse to ketamine seeking behavior in rats. METHODS: Male Sprague-Dawley rats were trained to respond for intravenous ketamine (0.5 mg/kg/infusion) self-administration or food pellets. The effects of lamotrigine on the motivation for ketamine or food were assessed using breakpoint test under a progressive ratio (PR) paradigm. Furthermore, the effects of lamotrigine on reinstatement of ketamine-seeking and food-seeking behaviors were examined after extinction. RESULTS: Lamotrigine significantly decreased the breakpoint for ketamine and prevented cue- and ketamine priming-induced reinstatement of ketamine seeking behavior. However, lamotrigine did not affect the breakpoint for food reinforcement, cue-induced reinstatement of food-seeking behavior, or spontaneous locomotor activity. CONCLUSIONS: Our data reveal that lamotrigine is capable of attenuating the reinforcing efficacy of ketamine and reducing ketamine craving and relapse risk, which lays the foundation for conducting clinical trials in patients with ketamine use disorder.

Capturing magnetic bead-based arrays using perpendicular magnetic anisotropy.

Designing and implementing means of locally trapping magnetic beads and understanding the factors underlying the bead capture force are important steps toward advancing the capture-release process of magnetic particles for biological applications. In particular, capturing magnetically labeled cells using magnetic microstructures with perpendicular magnetic anisotropy (PMA) will enable an approach to cell manipulation for emerging lab-on-a-chip devices. Here, a Co (0.2 nm)/Ni (0.4 nm) multilayered structure was designed to exhibit strong PMA and large saturation magnetization (M s ). Finite element simulations were performed to assess the dependence of the capture force on the value of M s . The simulated force profile indicated the largest force at the perimeter of the disks. Arrays of Co/Ni disk structures of (4-7) µm diameter were fabricated and tested in a microchannel with suspended fluorescent magnetic beads. The magnetic beads were captured and localized to the edge of the disks as predicted by the simulations. This approach has been demonstrated to enable uniform assembly of magnetic beads without external fields and may provide a pathway toward precise cell manipulation methods.