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Aim: To observe the effect of warming menstruation and analgesic herbal soup (WMAS) on the pathway of programmed cell death protein 1 and its ligand 1 PD-1/PD-L1 in rats with endometriosis model. Methods: A total of 90 mature female Wistar rats were randomly divided into 6 groups of 15 rats each. Of these, 5 groups were randomly selected for endometriosis molding and given high (HW group), medium (MW group) and low (LW group) doses of WMAS, western medicine (progesterone capsules, PC group) and saline gavage (SG group) respectively. The other group was a normal group (NM group), which was given saline gavage. The protein expression of PD-1 and PD-L1 on rat in eutopic and ectopic endothelium was detected by immunohistochemistry and the mRNA expression of PD-1 and PD-L1 in rats was detected by real-time fluorescence quantitative polymerase chain reaction (PCR). Results: The protein and mRNA expression of PD-1 and PD-L in the eutopic and ectopic endometrium of rats in the endometriosis group were higher than in the normal group (P <.05). The protein and mRNA expression of PD-1 and PD-L1 in the eutopic and ectopic endothelium of the HW, MW and PC groups were lower than in the SG group (P <.05). Conclusion: High expression of PD-1 and PD-L1 occurs in endometriosis, and WMAS can inhibit the immune signalling pathway PD-1/PD-L1, which may be available to inhibit the development of endometriosis.
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Endometriose , Humanos , Ratos , Feminino , Animais , Endometriose/tratamento farmacológico , Endometriose/genética , Endometriose/metabolismo , Menstruação , Antígeno B7-H1/metabolismo , Receptor de Morte Celular Programada 1/metabolismo , Ratos Wistar , RNA Mensageiro , AnalgésicosRESUMO
Plasma membrane intrinsic protein (PIP) is one of the largest subfamilies of Aquaporins (AQPs) and plays an important role in plant growth and development, and resistance to abiotic stress. In this study, the full length of the EuPIP1;1 cDNA was cloned from Eucommia ulmoides using the rapid amplification of cDNA ends (RACE) method. The EuPIP1;1 gene was induced by drought treatment and expressed in all tested tissues, with the highest expression level in fruit. The subcellular localization showed that EuPIP1;1 was located in the plasma membrane. Constitutive overexpression of EuPIP1;1 in Arabidopsisthaliana could promote leaf growth and development, and accelerate bolting and flowering. Six genes related to growth and flowering (AtPIF4, AtTCP14, AtCRY1, AtCRY2, AtFCA and AtFT) were significantly up-regulated in transgenic lines. Further, EuPIP1;1 gene improved resistance to drought and salt stress in transgenic Arabidopsis. Under drought and salt stress treatment, the transgenic lines had a higher germination rate and accumulation of osmotic substances, lower membrane damage, and could maintain ion homeostasis. Our results suggest that EuPIP1;1 plays an essential role in plant growth and development and in the response to drought and salt stress.
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Aquaporinas , Arabidopsis , Eucommiaceae , Aquaporinas/genética , Aquaporinas/metabolismo , Arabidopsis/metabolismo , DNA Complementar/metabolismo , Secas , Eucommiaceae/genética , Regulação da Expressão Gênica de Plantas , Folhas de Planta/genética , Folhas de Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Tolerância ao Sal/genética , Estresse Fisiológico/genéticaRESUMO
Growth-regulating factors (GRFs) are plant-specific transcription factors that play an important role in plant growth and development. In this study, fifteen GRF gene members containing QLQ and WRC domains were identified in Zanthoxylum armatum. Phylogenetic and collinearity analysis showed that ZaGRFs were closely related to CsGRFs and AtGRFs, and distantly related to OsGRFs. There are a large number of cis-acting elements related to hormone response and stress induction in the GRF gene promoter region of Z. armatum. Tissue-specific expression analysis showed that except for ZaGRF7, all the ZaGRFs were highly expressed in young parts with active growth and development, including terminal buds, seeds, and young flowers, suggesting their key roles in Z. armatum growth and development. Eight ZaGRFs were selected to investigate the transcriptional response to auxin, gibberellin and drought treatments. A total of six ZaGRFs in the NAA treatment, four ZaGRFs in the GA3 treatment, and six ZaGRFs in the PEG treatment were induced and significantly up-regulated. Overexpression of ZaGRF6 increased branching and chlorophyll content and delayed senescence of transgenic Nicotiana benthamiana. ZaGRF6 increased the expression of CRF2 and suppressed the expression of ARR4 and CKX1, indicating that ZaGRF6 is involved in cytokinin metabolism and signal transduction. These research results lay a foundation for further analysis of the GRF gene function of Z. armatum and provide candidate genes for growth, development, and stress resistance breeding of Z. armatum.
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Zanthoxylum , Longevidade , Filogenia , Melhoramento Vegetal , Extratos Vegetais/farmacologia , Folhas de Planta , Zanthoxylum/genéticaRESUMO
BACKGROUND: The study investigated the success rate of the great saphenous venous catheter placement performed by ultrasound-assisted technique compared with the conventional puncture method in infants and toddlers with congenital heart disease and aimed to assess the efficiency and feasibility of this method within the context of pediatric peripheral venous access. METHODS: We selected infants and toddlers who underwent congenital cardiac surgery in our medical center from June 1, 2020, to September 7, 2020, by convenience sampling. Children were stratified by the presence of the manifesting cardiac types (cyanotic or acyanotic heart disease). They were assigned to the conventional puncture method group or the ultrasound-assisted group through randomly blocked randomization. The primary outcome was the success rate of the first attempt. The second outcomes included the time to cannulation at the first attempt, the redirections of the first attempt, overall puncture time, and overall redirections of efforts. Besides, a binary logistic regression model was implemented to identify the possible variables related to the success rate of the first attempt. RESULTS: A total of 144 children in our medical center were recruited in the study. The success rate of the first attempt in the ultrasound-assisted group was higher than that of the conventional puncture method group in the stratification of cyanotic children (66.7% vs. 33.3%, P = 0.035). Among children of acyanotic kind, the difference in the success rate of the first attempt between the two groups was not significant (57.6% vs. 42.4%, P = 0.194). Overall puncture time (45.5 s vs. 94 s, P = 0.00) and the time to cannulation at the first attempt (41.0 s vs. 60 s, P = 0.00) in the ultrasound-assisted group was less than the conventional puncture method group. The ultrasound-assisted group also required fewer redirections of the first attempt (three attempts vs. seven attempts, P = 0.002) and fewer total redirections of efforts (two attempts vs. three attempts, P = 0.027) than the conventional puncture method group. The result of binary Logistic regression showed that the success rate of the first attempt was related to age (OR:1.141; 95% CI = 1.010-1.290, P = 0.034), the redirections of the first attempt (OR:0.698; 95% CI = 0.528-0.923, P = 0.012) and the saphenous venous width (OR:1.181; 95% CI = 1.023-1.364, P = 0.023). CONCLUSIONS: The ultrasound-assisted technique improves the saphenous venous cannulation sufficiently in children with difficult peripheral veins. The younger age is associated with a higher likelihood of peripheral venous difficulty. The ultrasound-assisted methods can effectively screen peripheral veins, e.g., selecting thicker diameter peripheral veins, making puncture less uncomfortable, and improving success rates. This method can be used as one of the effective and practical ways of peripheral venipuncture in children, especially in difficult situations. It should be widely applied as one of the alternative ultrasound techniques in the operating room. TRIAL REGISTRATION: ChiCTR.org.cn ( ChiCTR-2,000,033,368 ). Prospectively registered May 29, 2020.
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Cateterismo Venoso Central/métodos , Cardiopatias Congênitas/cirurgia , Punções/métodos , Veia Safena/diagnóstico por imagem , Ultrassonografia de Intervenção , Feminino , Humanos , Lactente , Masculino , Estudos Prospectivos , Estudos de AmostragemRESUMO
The abnormal deposition of the extracellular amyloid-ß peptide is the typical pathological hallmark of Alzheimer's disease. Strategies to reduce the amyloid-ß deposition effectively alleviate the neuronal degeneration and cognitive deficits of Alzheimer's disease. Danggui-Shaoyao-San has been considered a useful therapeutic agent known for the treatment of Alzheimer's disease. However, the mechanism of Danggui-Shaoyao-San for the treatment of Alzheimer's disease remains unclear. We investigated Danggui-Shaoyao-San's effect on amyloidosis and neuronal degeneration in an APP/PS1 mouse model. We found Danggui-Shaoyao-San alleviated the cognitive deficits in APP/PS1 mice. Additionally, Danggui-Shaoyao-San ameliorated the neuronal degeneration in these mice. Danggui-Shaoyao-San reduced the amyloidosis and amyloid-ß1-42 deposition in APP/PS1 mouse brain and down-regulated the receptor for advanced glycation end products, and up-regulated the level of low-density lipoprotein receptor-related protein-1. However, the protein expression of the ß-amyloid precursor protein, ß-Secretase and presenilin-1 (PS1) in the amyloid-ß production pathway, and the expression of neprilysin and insulin-degrading enzyme in the amyloid-ß degradation pathway were not altered. Our findings collectively suggest that Danggui-Shaoyao-San could ameliorate the amyloidosis and neuronal degeneration of Alzheimer's disease, which may be associated with its up-regulation lipoprotein receptor-related protein-1 and down-regulation of the receptor for advanced glycation end products.
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Doença de Alzheimer/tratamento farmacológico , Amiloidose/tratamento farmacológico , Disfunção Cognitiva/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Proteína-1 Relacionada a Receptor de Lipoproteína de Baixa Densidade/efeitos dos fármacos , Receptor para Produtos Finais de Glicação Avançada/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Regulação para Baixo/efeitos dos fármacos , Camundongos , Camundongos Transgênicos , Regulação para Cima/efeitos dos fármacosRESUMO
Indoleamine 2,3-dioxygenase 1 (IDO1) is an attractive therapeutic target for the treatment of cancer, chronic viral infections and neurological disorders characterized by pathological immune stimulation. Herein, a series of known metal-chelating ubiquinone derivatives were designed, synthesized and evaluated for the IDO1 inhibiting activities. The docking studies showed that the compounds 11, 16, 18 and coenzyme-Q1 exhibited different binding modes to IDO1 protein. Among these compounds, the most active compound is 16d with an IC50 of 0.13⯵M in enzymatic assay. The results reveal that a possible halogen bonding interaction between the bromine atom (3-Br) and Cys129 significantly enhances the inhibition activity against IDO1. This study provides structural insights of the interactions between ubiquinone analogues and IDO1 protein for the further modification and optimization.
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Desenho de Fármacos , Inibidores Enzimáticos/síntese química , Indolamina-Pirrol 2,3,-Dioxigenase/antagonistas & inibidores , Ubiquinona/análogos & derivados , Sítios de Ligação , Domínio Catalítico , Sobrevivência Celular/efeitos dos fármacos , Inibidores Enzimáticos/metabolismo , Inibidores Enzimáticos/farmacologia , Células HEK293 , Humanos , Indolamina-Pirrol 2,3,-Dioxigenase/metabolismo , Simulação de Acoplamento Molecular , Relação Estrutura-Atividade , Ubiquinona/metabolismo , Ubiquinona/farmacologiaRESUMO
Dielectric polymer nanocomposites have received keen interest due to their potential application in energy storage. Nevertheless, the large contrast in dielectric constant between the polymer and nanofillers usually results in a significant decrease of breakdown strength of the nanocomposites, which is unfavorable for enhancing energy storage capability. Herein, BaTiO3 nanowires (NWs) encapsulated by TiO2 shells of variable thickness were utilized to fabricate dielectric polymer nanocomposites. Compared with nanocomposites with bare BaTiO3 NWs, significantly enhanced energy storage capability was achieved for nanocomposites with TiO2 encapsulated BaTiO3 NWs. For instance, an ultrahigh energy density of 9.53 J cm-3 at 440 MV m-1 could be obtained for nanocomposites comprising core-shell structured nanowires, much higher than that of nanocomposites with 5 wt% raw ones (5.60 J cm-3 at 360 MV m-1). The discharged energy density of the proposed nanocomposites with 5 wt% mTiO2@BaTiO3-1 NWs at 440 MV m-1 seems to rival or exceed those of some previously reported nanocomposites (mostly comprising core-shell structured nanofillers). More notably, this study revealed that the energy storage capability of the nanocomposites can be tailored by the TiO2 shell thickness. Finite element simulations were employed to analyze the electric field distribution in the nanocomposites. The enhanced energy storage capability should be mainly attributed to the smoother gradient of dielectric constant between the nanofillers and polymer matrix, which alleviated the electric field concentration and leakage current in the polymer matrix. The methods and results herein offer a feasible approach to construct high-energy-density polymer nanocomposites with core-shell structured nanowires.
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Nano-IR technology was firstly employed on bamboo fiber research with purpose to further understand the fine structure of bamboo fiber cell wall. Chemical constituent distribution of bamboo fiber was studied, and the feasibility of the novel technology was discussed by comparing with other traditional methods. The results showed that Nano-IR technology, which has made a breakthrough on diffraction limit of traditional infrared spectroscopy, can acquire nano-scale infrared spectrum of bamboo cell wall in situ condition. The characteristic peak positions of Nano-IR spectrum is basically the same with that of microscopic FTIR spectrum, indicating that Nano-IR spectrum can reveal the chemical information of bamboo cell wall. The results of the present work suggested that nano-IR technology could be an effective research tool in research of nano chemical composition distribution of bamboo cell wall.
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Parede Celular , Espectrofotometria InfravermelhoRESUMO
OBJECTIVE: To investigate the effect of Chinese medicines using the warming Yang and removing blood stasis method on levels of matrix metalloproteinases (MMPs)/tissue inhibitor metalloproteinases (TIMPs) secreted by cultured endometrial cells from patients with endometriosis. METHODS: Ectopic and eutopic endometrial cells obtaind from 15 endometriosis patients were cultured in vitro, and divided randomly into five groups: high dose; moderate dose; low dose; nemestran; blank control. The three dose groups were treated with a decoction prepared according to the principle of warming Yang and removing blood stasis; nemestran and 0.9% NaCl were administered to the nemestran group and balnk control group, respectively. Eutopic endometrial cells obtaind from 10 hysteromyoma patients were cultured in vitro, as the normal control group, 0.9% NaCl were administered to the normal control group. Cell culture supernatants were collected and levels of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), tissue inhibitor metalloproteinase-1 (TIMP-1) and tissue inhibitor metalloproteinase-2 (TIMP-2) detected by enzyme-linked immuno sorbent assay (ELISA). RESULTS: Compared with the normal control group, levels of MMP-1, MMP-2, and MMP-9 in eutopic and ectopic endometrium cell supernatants in the blank control group were increased, whereas levels of TIMP-1 and TIMP-2 were decreased (P < 0.05). Compared with the blank control group, levels of MMP-1 and MMP-2 in ectopic and eutopic endometrium cell supernatants cultured in low-dose, middle-dose, and high-dose groups were decreased, whereas levels of TIMP-1 and TIMP-2 were increased significantly (P < 0.05). CONCLUSION: The warming Yang and removing blood stasis method affects expression of MMPs and TIMPs.
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Circulação Sanguínea/efeitos dos fármacos , Medicamentos de Ervas Chinesas/administração & dosagem , Endometriose/tratamento farmacológico , Endometriose/enzimologia , Endométrio/enzimologia , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Inibidor Tecidual de Metaloproteinase-2/metabolismo , Adulto , Células Cultivadas , Endometriose/genética , Endometriose/fisiopatologia , Endométrio/citologia , Endométrio/efeitos dos fármacos , Endométrio/metabolismo , Feminino , Humanos , Metaloproteinase 1 da Matriz/genética , Metaloproteinase 1 da Matriz/metabolismo , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/genética , Metaloproteinase 9 da Matriz/metabolismo , Inibidor Tecidual de Metaloproteinase-1/genética , Inibidor Tecidual de Metaloproteinase-2/genética , Adulto JovemRESUMO
Study on the modified mechanism of wood cell walls, it is very important for improving treatment reagents, optimizing treatment technology, and enhancing wood density, mechanical properties, dimensional stability, and so on. Samples of plantation Chinese fir were treated gradually with synthesized water-soluble low-molecular-weight phenol formaldehyde (PF) resins under vacuum and pressure. The correlated physical and chemical properties of the treated and untreated reference samples were determined by X-ray diffractometer (XRD), Fourier transform infrared spectrometer (FTIR), and nuclear magnetic resonance spectrometer(NMR) (Using method of Cross Polarization/Magic Angle Spinning for continuous testing) with high precision and resolution. The results showed that, after treated with water-soluble low-molecular-weight PF resin, the average values of crystallinity from the treated samples were decreased obviously, and the average reduction rate was 12.67%, 11.91% and 6.26%, respectively. Comparing water-soluble, low-molecular-weight PF resin modified Chinese fir with untreated reference samples, no new chemical shifts and characteristic peaks of functional groups from esters, ethers, etc. were present by using FTIR and ¹³C NMR spectrum. It was considered that there was no distinct chemical reaction between the water-soluble low-molecular-weight PF resin and Chinese Fir cell walls. But water-soluble low-molecular-weight PF resin could enter into the structure relatively loose, large size spaces, relatively area large amorphous regions in cell walls of Chinese fir tracheids, and form physical filling, which resulting in the decreasing of relative crystallinity. This study has important reference value for the development of new wood modification reagents and the optimization of wood modification process. The findings also provide important theoretical foundation for further proving the modification mechanisms of wood cell walls and enriching the modified theories of wood cell walls.
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Parede Celular , Cunninghamia/citologia , Formaldeído/química , Fenóis/química , Polímeros/química , Madeira , Espectroscopia de Ressonância Magnética , Peso MolecularRESUMO
Human parechoviruses (HPeVs) are widespread pathogens causing a wide spectrum of diseases. The prevalence and genetic diversity of HPeV in children with acute diarrhea in China is not well known. The purpose of this study was to investigate the epidemiological characteristics of HPeV in Guangzhou, China. A total of 328 stool specimens collected from children under the age of 5 years with acute diarrhea were tested for the presence of HPeV. Of these, 44 (13.4 %, 44/328) were HPeV positive, with the majority of the infected children (97.7 %, 43/44) being younger than two years of age. HPeV was more frequently detected during July and August. The epidemiological profile of co-infections was similar to that observed in a previous study. Six different HPeV genotypes, including HPeV1, -3, -4, -5, -6, and -14, were identified, and of these, HPeV14, a rarely reported genotype, was reported for the first time in children with acute gastroenteritis in China. In summary, this study clearly demonstrated that HPeV circulating in Guangzhou, China, is genetically diverse, including six genotypes, and it provides useful epidemiological data on the features of HPeV infection in this area.
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Gastroenterite/virologia , Parechovirus/isolamento & purificação , Infecções por Picornaviridae/virologia , Pré-Escolar , China/epidemiologia , Feminino , Gastroenterite/epidemiologia , Humanos , Lactente , Recém-Nascido , Masculino , Filogenia , Infecções por Picornaviridae/diagnóstico , Infecções por Picornaviridae/epidemiologiaRESUMO
This study reports the investigation on C60 bombardment on the carbon nanotube (CNT) by molecular dynamic (MD) simulations. We found that carbon nanotube nanobuds or nanotube-fullerene hybrid nanostructures can be formed by C60 bombardment. Different from the nanobuds in earlier studies, more structural patterns are found in the bombardment formed nanobuds and nanotube-fullerene hybrid nanostructures. In addition, the attaching strengths of the carbon nanobuds are explored, and results indicate that the junction between C60 and CNTs in the nanobuds is very stable. Moreover, we also found that the bombardment formed nanobuds and nanotube-fullerene hybrid nanostructures generally decrease the maximum tensile strength and Young's modulus of carbon nanotubes.
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BACKGROUND: Although interleukin-10 (IL-10) is a potent inhibitor of allergic diseases, the association between promoter -1082/-819/-592 polymorphisms and asthma susceptibility remains inconclusive. We sought to determine if IL-10 promoter -1082/-819/-592 polymorphisms contribute to asthma susceptibility and are associated with phenotypes of atopic asthma. METHODS: Systematic computerized searches were performed. Pooled odds ratios (ORs) and 95 % confidence intervals (CIs) were estimated by using random-effect and fixed-effect models, based on between-study heterogeneity. Subgroup analyses were performed according to age, ethnicity, and atopy. Publication bias was detected by funnel plot using Egger's test. RESULTS: A total of 4,716 asthmatic patients and 5,093 controls were included. The asthma susceptibility correlated significantly with IL-10 promoter gene -1082 polymorphism [OR (95 % CI) 1.26 (1.02, 1.55) for AA vs. AG + GG] and -592 polymorphism [OR (95 % CI) 1.12 (1.07, 1.34) for AC + AA vs. CC] (both P < 0.05), but not with -819 polymorphism (P > 0.05). Subgroup analyzes suggested that the AA versus AG + GG genotype of -1082A/G polymorphism and AC + AA versus CC genotype of -592A/C polymorphism contributed significantly to increased asthma susceptibility in adults [OR (95 % CI) 1.39 (1.03, 1.87) for -1082A/G and 1.53 (1.25, 1.87) for -592A/C polymorphism]. The Asian population [OR (95 % CI) 1.35 (1.1, 1.7) for -1082A/G and 1.4 (1.12, 1.64) for -592A/C polymorphism] and subjects with atopic asthma [OR (95 % CI) 1.49 (1.18, 1.88) for -1082A/G and 1.23 (1.01, 1.48) for -592A/C polymorphism] also had an increased susceptibility of asthma. No publication bias was detected. CONCLUSIONS: IL-10 promoter -1028A/G, -592A/C polymorphisms and their haplotypes, but not -819T/C polymorphism, correlate with asthma susceptibility.
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Povo Asiático/genética , Asma/genética , Interleucina-10/genética , Polimorfismo de Nucleotídeo Único , Regiões Promotoras Genéticas , Adulto , Fatores Etários , Asma/etnologia , Asma/imunologia , Estudos de Casos e Controles , Criança , Frequência do Gene , Predisposição Genética para Doença , Haplótipos , Humanos , Razão de Chances , Fenótipo , Fatores de RiscoRESUMO
OBJECTIVE: To study the correlation between dosage and curative effect of blood coagulation factor VIII in the prevention and treatment of haemophilia A in children and to determine the suitable dose for prevention of hemophilia in developing countries. METHODS: For different body weights of child patient, every time we used the same dosage of blood coagulation factor VIII (250 U each time, 3 times a week) and observed and recorded the number of hemorrhages in child patients. Then we compared the number of hemorrhages with children without treatment to determine the curative effect. According to the different body weights, we calculated the dosage of VIII factor of blood coagulation per kilogram (hereinafter referred to as the dose), and used Spearman correlation coefficient to study the correlation between dose and curative effect. RESULTS: The number of hemorrhages in 58 child patients before the treatment was 4.36 ± 1.78, while after the treatment was 2.22 ± 1.04 (t=7.91, P<0.001). The Spearman correlation coefficient of child patients of 5-10 U/kg was -0.421 (P=0.005); the Spearman correlation coefficient of child patients of 10-15 U/kg was -0.331 (P=0.030); the Spearman correlation coefficient of child patients over 15 U/kg was -0.16 (P=0.325). CONCLUSION: Prevention and treatment can significantly reduce the times of hemorrhage in hemophilia patients.
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Fator VIII/administração & dosagem , Fator VIII/uso terapêutico , Hemofilia A/terapia , Coagulação Sanguínea , Criança , Hemorragia/prevenção & controle , HumanosRESUMO
High intensity rainfall in southern China has led to soil erosion on sloping farmland, causing serious ecological and environmental problems. But how the interaction of rainfall factors and growth stages influence soil erosion and nitrogen loss on sugarcane-cultivated slope under natural rainfall have not been studied considerably. This study concentrated on the in situ runoff plot observation test. Surface runoff, soil erosion, and nitrogen loss under individual natural rainfall events during the different sugarcane growth stages (seedling stage (SS), tillering stage (TS), elongation stage (ES)) from May to September in 2019 and 2020 were recorded and measured. The effects of rainfall factors (intensity and amount) on soil erosion and nitrogen loss were quantified by path analysis. The influence of rainfall factors and sugarcane planting on soil erosion and nitrogen loss was analyzed. Surface runoff, soil erosion, and nitrogen loss on sugarcane-cultivated slope were 4354.1 m3/ha, 155.4 t/ha, and 25.87 kg/ha during 2019 to 2020, and were mainly concentrated in SS, accounting for 67.2%, 86.9%, and 81.9% of total surface runoff, soil erosion, and nitrogen loss, respectively. Nitrogen losses were mainly concentrated in surface runoff, accounting for 76.1% of total nitrogen loss, and the main form in surface runoff was nitrate nitrogen (NO3--N, 92.9%). Under individual rainfall events, surface runoff, soil erosion, and nitrogen loss changed with the changing of rainfall characteristics and sugarcane growth. Surface runoff and nitrogen loss were obviously affected by rainfall characteristics, while the soil erosion and nitrogen loss were affected by both rainfall characteristics and sugarcane growth stages. Path analysis indicated that maximum rainfall intensities at 15 min (I15) and 60 min (I60) were most significant to the production of surface runoff and soil erosion with direct path coefficients of 1.19 and 1.23, respectively. NO3--N and ammonium nitrogen (NH4+-N) losses in surface runoff were mostly influenced by maximum rainfall intensity at 30 min (I30) and I15 with direct path coefficients of 0.89 and 3.08, respectively. NO3--N and NH4+-N losses in sediment yield were mostly influenced by I15 and rainfall amount, and the direct path coefficients were 1.61 and 3.39, respectively. The main stage of soil and nitrogen loss was seedling stage, while the significant factors of rainfall characteristics affecting surface runoff, soil erosion, and nitrogen loss were quite different. The results provide theoretical support for soil erosion and quantitative rainfall erosion factors of sugarcane-cultivated slope in southern China.
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Saccharum , Solo , Nitrogênio/análise , Chuva , Movimentos da Água , Grão Comestível/química , ChinaRESUMO
The effects of vegetable planting on soil loss and nutrient loss, runoff, soil erosion, and nitrogen (ammonium nitrogen and nitrate nitrogen) losses under individual rainfalls of fruit- and leaf-vegetable fields between April to October in 2021 were observed using in-situ observation testing. The results showed that: â the runoff, erosion, and nitrogen loss of the fruit-vegetable pattern (eggplant-chili) were 1.27-2.00 times those under the leaf-vegetable pattern (leaf lettuce-sweet potato leaves), especially under the second season vegetable period. Those losses under the second season vegetable accounted for 50.86%-68.83% of the total losses under different vegetable patterns, which were approximately 1.03-2.04 times those under the first season vegetable. The runoff, erosion, and nutrient loss of vegetable fields under different treatments were both concentrated in June and July, and the nitrogen loss was mainly in the form of nitrate nitrogen with surface runoff. â¡ The runoff, erosion, and nutrient losses under individual rainfalls of vegetable fields under different treatments fluctuated among the vegetable growing season, and the losses were mainly concentrated in several typical rainfall events. On the whole, the loss and concentration of nitrate and ammonium nitrogen in runoff and erosion sediment of vegetables in the first season were lower than those in the second season. The runoff, erosion, and loss of ammonium nitrogen and nitrate nitrogen of fruit-vegetable were higher than those of leaf-vegetable. ⢠Both rainfall amount and maximum 30 min rainfall intensity had significantly positive effects on runoff, soil loss, and nitrogen loss. Runoff, erosion, and nutrient losses under different vegetable patterns were mainly generated by moderate rain, heavy rain, and heavy rainstorms, which accounted for 29.58%-46.68%, 24.54%-36.79%, and 24.01%-39.13% of the total losses, respectively. The results also showed that soil erosion and nutrient losses generated by different rainfall grades were obviously different for the fruit- and leaf-vegetable treatments. The results indicated that the vegetable pattern had significant impacts on soil loss and nutrient loss, and the leaf-vegetable pattern could reduce soil erosion and nutrient loss compared with the fruit-vegetable pattern. Furthermore, for different vegetable patterns and vegetable growing seasons, the effects of rainfall on soil loss and nutrient loss were quite different. The results of this study were helpful in clarifying the soil erosion and nutrient loss characteristics of vegetable fields in South China.
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Erosão do Solo , Verduras , Nitrogênio/análise , Nitratos , Fósforo/análise , Movimentos da Água , Solo , Chuva , ChinaRESUMO
The connectivity of the pore/fracture system is the key to CO2 injection and CH4 production, which is of great significance in analyzing the correlation and weight of the influencing factors affecting the connectivity on the microscale. First, the 3D reconstruction of the coal reservoir is realized. Second, the characteristic parameters of pore/fracture structures are analyzed. Next, the characteristics of absolute permeability are analyzed, and then the correlation and weight analysis of the influencing factors are realized. Finally, the schematic analysis and application discussion of the influencing factors are carried out. The results show that porosity is the key factor restricting fluid migration. The heterogeneity of the reservoir can be characterized by the volume changes of the pore/fracture, organic matter, and mineral. The interconnected pores/fractures are mainly distributed in sheets and bands. The coordination number ranges from 1 to 15. The Ferret diameter is 0-10 µm. The tortuosity is 2.27111, 1.9034, 3.98522, and 3.51516, respectively, and the Euler characteristic number is 0.931868, 0.974719, 0.921144, and 0.897697, respectively. The permeability of the SH and YW samples is higher than that of the RL and PY samples. The single weight of the influencing factor is as follows: coordination number > Ferret diameter > Euler characteristic number > porosity > tortuosity. The analysis area of the comprehensive evaluation score of the influencing factors and the permeability value can be divided into three grades. There is a positive correlation among the coordination number, the quantity equilibrium of pores and throats, and the connectivity. The shape factor gradually increases with the increase of the Ferret diameter. The reservoir permeability is indirectly characterized by the coordination number, Ferret diameter, tortuosity, Euler characteristic number, and shape factor. This study can provide new ideas for clarifying the correlation degree and weight value of the characteristic parameters and can enrich the development of 3D digital core and CO2-ECBM technology.
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Our previous study demonstrated that the zinc (Zn) proteinate with moderate chelation strength (Zn-Prot M) enhanced the Zn absorption in the small intestine partially via increasing the expression of some Zn and amino acid transporters in the duodenum of broilers. However, it remains unknown whether the Zn-Prot M could also regulate the expression of related transporters in the jejunum and ileum of broilers in the above enhancement of Zn absorption. The present study was conducted to investigate the effect of the Zn-Prot M on the expression of related transporters in the jejunum and ileum of broilers compared to the Zn sulfate (ZnS). Zinc-deficient broilers (13-d-old) were fed with the Zn-unsupplemented basal diets (control) or the basal diets supplemented with 60 mg Zn/kg as ZnS or Zn-Prot M for 26 d. The results showed that in the jejunum, compared to the control, supplementation of the organic or inorganic Zn increased (P < 0.05) mRNA and protein expression of b0,+-type amino acid transporter (rBAT), Zn transporter 10 (ZnT10), and peptide-transporter 1 (PepT1) mRNA expression and Zn transporter 7 (ZnT7) protein expression on d 28, while y+L-type amino transporter 2 (y+LAT2) mRNA and protein expression, and protein expression of ZnT7 and ZnT10 on 28 d and zrt-irt-like protein 3 (ZIP3) and zrt-irt-like protein 5 (ZIP5) on d 39 were higher (P < 0.05) for Zn-Prot M than for ZnS. In the ileum, Zn addition regardless of Zn source up-regulated (P < 0.05) mRNA expression of Zn transporter 9 (ZnT9) and ZIP3, ZIP5, and y+LAT2 protein expression on d 28, and PepT1 mRNA and protein expression, ZIP3 and y+LAT2 mRNA expression and ZnT10 protein expression on d 39. Furthermore, Zn transporter 4 (ZnT4) and ZnT9 mRNA expression and Zn transporter 1 (ZnT1) protein expression on d 28, and y+LAT2 mRNA expression and ZnT10 and PepT1 protein expression on d 39 were higher (P < 0.05) for Zn-Prot M than for ZnS. It was concluded that the Zn-Prot M enhanced the expression of the ZnT1, ZnT4, ZnT9, ZnT10, ZIP3, ZIP5, y+LAT2, and PepT1 in the jejunum or ileum of broilers compared to the ZnS.
Assuntos
Galinhas , Jejuno , Compostos Organometálicos , Zinco , Animais , Sistemas de Transporte de Aminoácidos/genética , Sistemas de Transporte de Aminoácidos/metabolismo , Galinhas/genética , Galinhas/metabolismo , Íleo/metabolismo , Jejuno/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Zinco/metabolismo , Compostos Organometálicos/metabolismoRESUMO
Resistivity low-contrast oil pays are a kind of unconventional oil resource with no obvious difference in physical and electrical properties from water layers, which makes it difficult to be identified based on the characteristics of the geophysical well logging response. In this study, the support vector machine (SVM) technology was used to interpret the resistivity low-contrast oil pays in Chang 8 tight sandstone reservoir of Huanxian area, Ordos Basin. First, the input data sequences of logging curves were selected by analyzing the relationship between reservoir fluid types and logging data. Then, the SVM classification model for fluid identification and SVR regression model for reservoir parameter prediction were constructed. Finally, these two models were applied to interpret the resistivity low-contrast oil pays in the study area. The application results show that the fluid recognition accuracy of the SVM classification model is higher than that of the logging cross plot method, back propagation neural network method and radial basis function neural network method. The calculation accuracy of permeability and water saturation predicted by the SVR regression model is higher than that based on the experimental fitting model, which indicates that it is feasible to carry out logging interpretation and evaluation of the resistivity low-contrast oil pays by the SVM method. The research results not only provide an important reference and basis for the review of old wells but also provide technical support for the exploration and development of new strata.
RESUMO
Inner wall temperature of ladle is closely related to the quality of steelmaking and control of steel-making tapping temperature. This article adopts a rotating platform to drive an infrared temperature sensor and a laser sensor to scan the temperature field distribution of the ladle inner wall at the hot repair station, where the scanning laser sensor obtains coordinates of each measured point. Because of measuring errors of infrared thermal radiation caused by emissivity uncertainty of the ladle inner wall surface, this article proposes a method for temperature measurement based on Monte Carlo model for effective emissivity correction of each measured point. In the model, we consider the ladle and fire baffle as a cavity. By calculation of the model, the effect of distance from the fire baffle to the ladle and the material surface emissivity of the ladle inner wall on the effective emissivity of the cavity are obtained. After that, the effective emissivity of each measured point is determined. Then the scanning temperature of each measured point is corrected to real temperature. By field measuring test and verification contrast, the results show that: the maximum absolute error of the method in this article is 4.7 °C, the minimum error is 0.6 °C, and the average error is less than 2.8 °C. The method in this article achieves high measurement accuracy and contributes to the control of metallurgical process based on temperature information.