Rapid increase in contaminant burdens following loss of body condition in canvasbacks (Aythya valisineria) overwintering on the Lake St. Clair region of the Great Lakes.

Overwintering canvasbacks were collected in the Lake St. Clair region of the Great Lakes in the winter of 2008/09 and livers were analyzed for organochlorines, mercury (Hg), and selenium (Se). We found dramatic increases in hepatic concentrations of Hg, Se, sum PCBs, p,p'-DDE, and other organochlorines in canvasbacks in which concentrations in February were greater than concentrations in November when overwintering ducks arrived in the study area. Increases in contaminant burdens were generally greatest between December and January which also coincided with the period when ducks from Lake St. Clair (LSC) moved following freeze-up of the Lake to forage on the St. Clair River (SCR), an area of known historic contamination, and upstream of LSC. Body condition estimated using body metrics and measured using lipid reserves (after controlling for body size) increased in LSC ducks but subsequently decreased in SCR ducks. This rapid loss of body condition through loss of lipid reserves was one factor likely driving the dramatic increase in contaminant burdens and particularly for organochlorines which were inversely related to body condition in SCR ducks. Increased exposure due to foraging in closer proximity to contaminant sources and changes in diet associated with the movement of ducks may have also contributed to temporal trends. Concentrations overall were below those associated with toxicity with the exception of Se for which 30% of ducks exceeded the Se threshold that is considered elevated and one duck exceeded the threshold associated with possible toxicity. Fitness consequences of reduced lipid reserves include reduced survival, delayed migration, reduced breeding propensity, and transfer of contaminant burdens to eggs. Food availability, ice cover, and movements of canvasbacks are additional factors influencing contaminant accumulation and lipid reserves in waterfowl utilizing this important wintering location.

Effects of halogenated contaminants on reproductive development in wild mink (Neovison vison) from locations in Canada.

The concept of the Anthropocene, that humans are now re-engineering global ecosystems, is in part evidenced by the pervasive pollution by persistent organic pollutants (POPs). Certain POPs are hormone mimics and can disrupt endocrine and hence reproductive processes, shown mainly by laboratory studies with model species. There are, in contrast, fewer confirmations of such disruption from eco-epidemiological studies of wild mammals. Here we used the American mink (Neovison vison) as a sentinel species for such a study. Over the period 1998-2006, 161 mink carcasses were obtained from commercial trappers in the Canadian provinces of British Columbia and Ontario. Mink were aged, sexed, measured, and body condition assessed. Livers were analyzed either individually or pooled for organochlorine (OC) pesticides, polychlorinated biphenyls (PCBs), and subsets for polybrominated diphenyl ethers (PBDEs). We primarily addressed whether contaminants affected male reproductive development by measuring baculum size and assessing the influences of age and body condition. We also considered the influence of spatial variation on relative exposure and size of baculum. Statistical models separated by age class revealed that significant relationships between baculum length or mass and juvenile mink were mostly positive, whereas for adults and first year mink they were mostly negative. A significant negative relationship for adult mink was determined between DDE and both baculum length and mass. For juvenile mink we found significant positive relationships between ∑PCBs, DDE and ∑PBDEs with baculum length. Our results provide some indication of negative effects of halogenated contaminants on male reproductive development in wild mink, and the most likely candidate chemical is the confirmed anti-androgenic compound, DDE, rather than PCBs or other compounds.

Organochlorine contaminants in wild mink from the lower Great Lakes basin, Canada, 1998-2006.

Organochlorine contaminants were measured in livers of wild mink (Neovison vison) trapped in the lower Great Lakes basin from 1998 to 2006. To assess exposure and potential risk in mink feeding on Great Lakes biota, concentrations of contaminants were compared in mink trapped within 7.8 km of the shoreline as well as at inland sites (i.e., 8-40 km). Overall, significant spatial variation in mean hepatic concentrations of sum PCBs and seven other organochlorines was found in mink from 13 Great Lakes sites, many of which are within the Great Lakes Areas of Concern. Mean sum PCB concentrations, on a lipid weight basis, ranged from 2 µg/g in mink from inland Lake Ontario sites to 44 µg/g in mink from western Lake Erie. Concentrations of other organochlorines in mink were generally low. Mink from western Lake Erie had the highest mean cumulative organochlorine burdens dominated largely by PCBs. A significant age effect was found with 1-year-old mink having significantly higher PCB burdens than mink less than 1 year in age. With respect to published PCB threshold effect concentrations, some mink exceeded those associated with effects on reproduction and survival as well as the presence of jaw lesions. This was most consistently found in western Lake Erie where the health of populations of wild mink may be adversely affected and where no mink 2 years of age or older were collected.

Mercury and other heavy metals in free-ranging mink of the lower Great Lakes basin, Canada, 1998-2006.

Mercury concentrations were examined in livers of free-ranging mink (Neovison vison) trapped in the lower Great Lakes basin from 1998 to 2006. Significant geographic variation in total mercury levels was found in mink from 13 Great Lakes locations, many of which are within Great Lakes Areas of Concern (AOCs). Total mercury levels in mink from these locations were generally low to moderate, with means (±SE) ranging from 0.99 (±0.36) µg/g dry weight in mink from inland Lake Erie to 7.31 (±1.52) µg/g in mink from Walpole Island in the St. Clair River AOC. Overall, mercury exposure was highest in mink trapped in locations associated with large riverine wetlands. Total mercury concentrations were negatively related to the percentage of mercury present as methylmercury and positively related to selenium concentrations, consistent with increasing demethylation of methylmercury with increasing total mercury concentrations. One-year old mink had significantly higher levels of mercury and selenium than mink under 1 year but similar to mink 2 years of age and older. Mercury levels were below those associated with mercury toxicity. Concentrations of cadmium, lead and arsenic were low and largely associated with environmental background levels. Significant age and sex effects were found for cadmium. Lead levels were significantly lower in mink infected by the lung nematode parasite compared to uninfected animals. Further studies of biomagnification of methylmercury in wetland environments, where top predators such as mink may be an increased risk of exposure, are warranted.