[An immunoenzyme test in the diagnosis of opisthorchiasis and in seroepidemiological research]. / Immunofermentnyi test v diagnostike opistorkhoza i seroépidemiologicheskikh issledovaniiakh.

An indirect method for the determination of antibodies to Opisthorchis antigens has been used in the elaboration of enzyme immunoassay for opisthorchiasis diagnosis. A visual method for the assessment of the results has been suggested. Its diagnostic efficacy has been determined. With the study of 1722 sera of the control subjects, the test specificity was 97.5%. Test sensitivity in the acute stage of the disease (40 patients) was about 100%, in the chronic stage (435 patients) it depended on oocyst production by the parasite. With less than 10 oocysts per I g feces, test sensitivity did not exceed 34.3%, with more than 1000 oocysts per I g feces it reached 94.4%. Mass population screenings, using the test in question, in 2 opisthorchiasis foci with different levels of endemia have shown that their results correspond to the data of copro-ovoscopy. The use of the test is most promising for the diagnosis of early phase of opisthorchiasis and the assessment of epidemiological situation in the foci.

[The effectiveness of an immunoenzyme test with homologous and heterologous antigens in clonorchiasis]. / Effektivnost' immunofermentnogo testa s gomologichnym i geterologichnym antigenam pri klonorkhoze.

An enzyme immunoassay with homologous and heterologous antigens has been elaborated for the diagnosis of clonorchiasis. 251 blood serum samples from patients with clonorchiasis have been studied, as well as 230 samples from patients with geohelminthiases obtained from North Vietnam provinces and 299 samples from Moscow donors. The test sensitivity and specificity were 78.72% and 94.52%, respectively. The dependence of test sensitivity on the intensity of infection has been noted. The calculation of the diagnostic value of enzyme immunoassay makes it possible to use it with respect to the level of the population invaxon: in low level of invaxon (below 32%) D = 2.11M + 0.06, in invaxon level above 32%, D = 1.74M + 0.04. The use of a homologous antigen enhances the test sensitivity.

[Experience with the parallel use of immunoenzyme analysis and copro-ovoscopy for assessing the clonorchiasis infectivity of the population of Khabarovsk Territory]. / Opyt parallel'nogo ispol'zovaniia immunofermentnogo analiza i koproovoskopii dlia otsenki porazhennosti naseleniia klonorkhozom v Khabarovskom krae.

The paper describes how enzyme immunoassay (EIA) and copro-ovoscopy are concurrently used to define the spread of clonorchiasis among the population of the Nanaisk District in the Khabarovsk Territory. It shows the efficiency of EIA in seroepidemiological surveys and the possibility of its use in endemic areas. The sensitivity of EIA to determine antibodies to Clonorchis antigens was 70.58% and its specificity was 98.4%. The assay is recommended for wide application in clinical and epidemiological practice in the foci of the disease. The total affliction of the population, which was calculated by 2 techniques is 33.7% +/- 3.0.