Comparison of APACHE II and APACHE IV score as predictors of mortality in patients with septic shock in intensive care unit: A prospective observational study.

Background and Aims: Prediction of outcome in intensive care unit (ICU) patients is of imperative importance. Our aim was to assess and compare the performance of Acute Physiology and Chronic Health Evaluation (APACHE) II and APACHE IV scores in predicting mortality in adult patients suffering from septic shock admitted to our ICU. Material and Methods: This was a prospective observational study conducted in a 14-bedded medical ICU of a tertiary care center from January 2019 to March 2020; 128 patients suffering from septic shock were included and APACHE II and IV scores were calculated. We also calculated the predicted and actual mortality rates and standardized mortality ratios. The receiver operating characteristic curves were used to assess discrimination. Results: Out of the 128 patients, 63 patients (49.21%) died. The mean (± standard deviation) admission APACHE II score was 16.7 ± 5.53, while the mean APACHE IV score was 67.25 ± 25.99. The non-survivors had significantly higher APACHE II and IV scores when compared to those who survived (P < 0.001). APACHE II had a slightly better discriminative power (with the area under the Receiver operating characteristic (ROC) curve of 0.78) than APACHE IV (with the area under the ROC curve of 0.74). The mean predicted mortality rate (PMR) of the patient population calculated on the basis of the APACHE II scoring system was 22.46 ± 15.76, and the mean PMR calculated as per the APACHE IV scoring system was 11.64 ± 15.59. Conclusion: Both APACHE II and APACHE IV underestimated mortality in septic shock patients. Both APACHE II and APACHE IV were comparable in differentiating survivors from non-survivors. However, there was a good correlation between the two models.

Zwitterionic Luminescent 2D Metal-Organic Framework Nanosheets (LMONs): Selective Turn-On Fluorescence Sensing of Dihydrogen Phosphate.

While a plethora of organic linkers based on carboxylic acids have been utilized in the construction of MOFs, zwitterionic linkers that typify the attributes of naturally occurring amino acids have been exploited only scarcely to the best of our knowledge. Zwitterionic interior characteristics should be expected to impart unique properties to the resultant MOFs with a high potential to interact with guest species through electrostatic interactions. In our investigations with bis(p-carboxyphenyl)imidazolylarenes as a novel class of linkers for the development of functional MOFs, we have found that bisimidazole-tetracarboxylic acid H4DMBI undergoes metal-assisted self-assembly with Zn(NO3)2 to yield a layered MOF (Zn-DMBI). In the latter, the linker serves as a two-connecting linker with imidazoles and carboxylic acids behaving as zwitterions. The layers are offset stacked in the crystal structure and are bound firmly by hydrogen bonds between imidazolium and carboxylate ions. Such a packing precludes fluorescence from being observed due to self-quenching. However, exfoliation into zwitterionic 2D metal-organic nanosheets (MONs) by sonication in methanol for 1 h liberates palpable fluorescence. Furthermore, the suspension of luminescent MONs (LMONs) in methanol permits selective sensing of anions; in particular, dihydrogen phosphate (H2PO4-) that is complementary to the zwitterions in terms of hydrogen bond donor and acceptor sites is observed with fluorescence enhancement by 120%, leading to its detection at a sub-parts-per-million (0.13 ppm) level. Thus, access to zwitterionic 2D MONs and their application for selective anion sensing with "turn-on" fluorescence are demonstrated by a rational de novo bottom-up approach.

Topical vs. intravenous administration of tranexamic acid to minimize blood loss in abdominal hysterectomy perioperatively: A randomized controlled study.

Background and Aims: Topical application of tranexamic acid (TXA) to bleeding wound surfaces is rapidly gaining recognition and currently a topic of further research in patients undergoing abdominal hysterectomy. The aim of the study was to compare the efficacy of topical vs. intravenous (i.v.) administration of TXA in reducing perioperative blood loss in patients undergoing abdominal hysterectomy. Material and Methods: A double-blinded parallel-group randomized controlled study was conducted in a tertiary teaching institute. Group 1 (n = 25) received 10 mg.kg-1 i.v. bolus of TXA after induction followed by infusion of 1 mg.kg-1.h-1 of TXA, in 50 ml of normal saline (NS), till the completion of surgery and just before closure of peritoneum 100 ml of NS was applied topically over the raw surface. Group 2 (n = 25) received 50 ml of NS over 10 min after induction, followed by infusion of 50 ml of NS, till the completion of surgery and just before closure of peritoneum, 1.5 g of TXA mixed in 100 ml of NS was applied topically over the raw surface. The primary outcome was total perioperative blood loss (intraoperative plus 24 h postoperative). The secondary outcomes included change in hemoglobin concentration postoperatively at 12 h, 24 h; need for blood/blood product transfusion; amount of blood/blood product transfused and side effects of TXA. Results: Total perioperative blood loss was 312 ± 106.65 ml in group 1 and 325 ± 89.90 ml in group 2 (p = 0.659). It was found that the mean reduction in hemoglobin was 0.7 g.dl-1 and 0.54 g.dl-1 in group 1 and 0.67 g.dl-1 and 0.44 g.dl-1 in group 2 at 12 h and 24 h respectively, with no significant intergroup difference. Conclusion: Administration of TXA topically is as efficacious as TXA administered i.v. to minimize perioperative blood loss in patients undergoing abdominal hysterectomy.

Temperature-Dependent Emission and Turn-Off Fluorescence Sensing of Hazardous "Quat" Herbicides in Water by a Zn-MOF Based on a Semi-Rigid Dibenzochrysene Tetraacetic Acid Linker.

A zinc metal-organic framework, i.e., Zn-MOF (Zn-DBC), with ca. 27% solvent-accessible void volume was synthesized from a rationally designed tetraacid based on sterically insulated dibenzo[g,p]chrysene core; the latter inherently features concave shapes. Due to rigidification of the fluorophore in the MOF, Zn-DBC exhibits a respectable fluorescence quantum yield of ca. 30% in the solid state. The fluorescent and water-stable Zn-DBC MOF was found to display intriguing temperature-dependent emission behavior with an activation barrier of 1.06 kcal/mol for radiationless deactivation from the singlet-excited state. It is shown that the Zn-MOF can be employed as an efficient sensory material for detection of hazardous "quat" dicationic herbicides in water by diffusion-limited "turn-off" fluorescence. Due to confinement of the cationic guest analytes within the pores of the MOF, the fluorescence quenching via excited-state charge transfer mechanism is shown to depend on the molecular size of the analyte in addition to the redox potentials. Remarkably, Zn-DBC permits sensing of DQ, a well-known toxic "quat" herbicide, with a detection limit as low as 2.8 ppm in water. The unique structural attributes of the Zn-MOF for highly efficient fluorescence sensing of toxic herbicides in water are thus exemplified for the first time.

Does an acute pain service improve the perception of postoperative pain management in patients undergoing lower limb surgery? A prospective controlled non-randomized study.

BACKGROUND AND AIM: An acute pain service (APS) has been running in our institute since April 2013 and is managed by the Department of Anesthesia and Intensive Care. However, it is not clear to what extent the patients feel benefited from the APS. The aim of the study was to compare the perception of postoperative pain management in patients receiving care under APS with those receiving routine postoperative pain relief following lower limb surgery. MATERIAL AND METHODS: This was a prospective, hospital-based, controlled non-randomized study. American Society of Anesthesiologists (ASA) grades I-III patients with age 18-75 years undergoing lower limb orthopedic surgery were prospectively recruited into APS (index group) or routine postoperative care (control group) (n = 55 each). Postoperatively, American Pain Society Patient Outcome Questionnaire-Revised (APS-POQ-R) and Short Form (SF-12) were used to evaluate the outcome of postoperative pain management at 24 h and health-related quality of life after 4 weeks respectively. RESULTS: Both groups were comparable in terms of demographic data. Patients in the index group had statistically significant better perception of care than the control group. Index group scored significantly higher than control group on median patient satisfaction score (9; interquartile range [IQR] [7-10] vs. 5 [3-6]; P < 0.001). In index group, there was significant reduction of worst pain in first 24 h along with decreased frequency of severe pain. CONCLUSION: Implementation of acute pain service plays an important role in improving the quality of postoperative pain relief, perception of care, and patient satisfaction.

Redox-Reversible 2D Metal-Organic Framework Nanosheets (MONs) Based on the Hydroquinone/Quinone Couple.

2D metal-organic nanosheets (MONs), akin to graphene, have aroused immense contemporary interest. In our quest to develop functional 2D MONs based on organic linkers designed de novo, we reasoned that benzene-tetrabenzoic acid, which has been exploited tremendously in the construction of pillared metal-organic frameworks (MOFs), could be maneuvered readily to access redox-active MONs based on the benzoquinone/hydroquinone redox couple. Herein, we show that the self-assembly of 2,3,5,6-tetrakis(p-carboxyphenyl)hydroquinone H4 BTA with Zn(NO3 )2 does lead to 2D metal-organic nanosheets that stack down the y axis, affording a layered Zn MOF. Although the crystals of the latter do not exhibit a discernible chemically induced redox switching behavior, the 2D MONs accessed by ultrasound-induced liquid-phase exfoliation (UILPE) lend themselves to a facile redox switching behavior. Treatment of a dispersion of the 2D MONs in methanol with phenyliodine(III) diacetate (PIDA) results in the oxidation of the hydroquinone core to benzoquinone. Remarkably, the latter can be reverted to the former by treatment with ascorbic acid as a reducing agent; indeed, the redox process can be made out by the naked eye. The results constitute the first example of chemically induced redox switching of 2D MONs. In view of emergent applications of 2D materials in general and MONs in particular, for example, improvement of the performance of membranes in separations by doping with MONs, the redox-switchable property may lead to the development of unique materials with heretofore unexplored potential.

Serotonin Syndrome in ICU-A Road Less Traveled.

A potentially life-threatening adverse drug reaction, serotonin syndrome (SS) may occur in patients on serotonin agonist medications. Medications responsible for SS include commonly prescribed antidepressants, anxiolytics, analgesics and antiemetics. This syndrome has been mentioned in various scenarios in perioperative setting but rarely acknowledged in intensive care unit (ICU) setting. Multiple comorbities leading to polypharmacy and serotoninergic medications place elderly patients, particularly at higher risk of SS. We wish to report a case of SS in an elderly male with depression who developed SS and required ICU admission. Most crucial point is to be able to recognize this entity whenever a patient on multiple drugs with altered sensorium is admitted to ICU. HOW TO CITE THIS ARTICLE: Jindal S, Gombar S, Jain K. Serotonin Syndrome in ICU-A Road Less Traveled. Indian J Crit Care Med 2019;23(8):376-377.

Development of a 3D printable maxillofacial silicone: Part II. Optimization of moderator and thixotropic agent.

STATEMENT OF PROBLEM: Conventionally, maxillofacial prostheses are fabricated by hand carving the missing anatomic defect in wax and creating a mold into which pigmented silicone elastomer is placed. Digital technologies such as computer numerical control milling and 3-dimensional (3D) printing have been used to prepare molds, directly or indirectly, into which a biocompatible pigmented silicone elastomer can be placed. PURPOSE: The purpose of this in vitro study was to develop a silicone elastomer that could be 3D printed directly without a mold to create facial or body prostheses by varying its composition. MATERIAL AND METHODS: The room temperature vulcanizing silicone composition was divided into 2 components which were mixed 1:1 to initiate polymerization in the printer before printing began. Different types of moderators and thixotropic agents were used, and the base composition was varied to obtain 11 formulations. The specimens were printed and polymerized from these formulations and tested for tear and tensile strength and hardness. Ten readings of the specimens were recorded for tear and tensile strength and 6 for hardness. Results were analyzed using ANOVA (α=.05). Visual assessment of uncured printed specimens was undertaken for 5 formulations to assess any differences in their ability to hold their shape after printing. RESULTS: The tear and tensile strength of the 11 formulations with varying moderators, thixotropic agents, and base compositions were statistically similar to each other (P>.05). Five of 11 formulations were chosen for the visual assessment as they had sufficient thixotropic agent to avoid slumping while printing. The specimens showed varied slumping behavior until they polymerized. The filler content was increased in the selected formulation, and the tear and tensile strength of the formulation was increased to 6.138 kNm-1 and 3.836 MPa; these increases were comparable to those of commercial silicones currently used for the fabrication of facial prostheses. CONCLUSIONS: The optimum combination of mechanical properties implies the use of one of the formulations as a suitable material for the 3D printing of facial prostheses.

Development of a 3D printable maxillofacial silicone: Part I. Optimization of polydimethylsiloxane chains and cross-linker concentration.

STATEMENT OF PROBLEM: Conventionally, maxillofacial prostheses are fabricated by hand carving the missing anatomic defect in wax and creating a mold into which pigmented silicone elastomer is placed. Digital technologies such as computer numerical control (CNC) milling and 3-dimensional (3D) printing have been used to prepare molds directly or indirectly into which a biocompatible pigmented silicone elastomer is placed. PURPOSE: The purpose of this in vitro study was to develop a silicone elastomer by varying composition that could eventually be 3D printed directly without a mold to create facial/body prostheses. MATERIAL AND METHODS: The silicone was composed of polydimethylsiloxane (PDMS), filler, catalyst, and cross-linker. Four types of base silicone polymers were prepared with different PDMS molecular weight combinations with long, medium, and short chain length PDMS. The effect of the cross-linker (2.5% to 12.5%) content in these bases was assessed for the effect upon the mechanical properties of the elastomer. Ten readings were made for each formulation, and differences in the means were evaluated with a 2-way ANOVA (α=.05). RESULTS: Variations in silicone composition resulted in hardness from 6.8 to 28.5 durometer, tensile strength from 0.720 to 3.524 kNm-1 and tear strength from 0.954 to 8.484 MPa. Significant differences were observed among all formulations (P<.05). These formulations have mechanical properties comparable with the commercial silicones currently used for the fabrication of facial prostheses. The formulation with 5% cross-linker content and high content of long-chain PDMS chains with optimum mechanical properties was chosen for further development. CONCLUSIONS: The optimum combination of mechanical properties implies the use of one of these formulations for further evaluation in a 3D printer capable of actively mixing and extruding 2-component, room temperature vulcanization silicone.

Bacterial diversity and real-time PCR based assessment of linA and linB gene distribution at hexachlorocyclohexane contaminated sites.

The disposal of hexachlorocyclohexane (HCH) muck has created large number of HCH dumpsites all over the world from where the harmful HCH isomers are leaking into the environment. Bacteria have evolved at such contaminated sites that have the ability to degrade HCH. Degradation of various HCH isomers in bacterial strains is mediated primarily by two genes: linA and linB which encode dehydrochlorinase and haloalkane dehalogenase respectively. In this study we explored one such highly contaminated HCH dumpsite located in Lucknow, Uttar Pradesh, India. To assess the biostimulation potential of the contaminated site, microbial diversity study and real-time PCR based quantification of lin genes was carried out. The soil samples from dumpsite and surrounding areas were found to be highly contaminated with HCH residue levels as high as 1.8 × 10(5) mg kg(-1). The residues were detected in areas upto 13 km from the dumpsite. Sphingomonads, Chromohalobacter, and Marinobacter were the dominant genera present at the dump-site. Role of Sphingomonads in HCH degradation has been well documented. The highest copy numbers of linA and linB genes as determined using real-time PCR were 6.2 × 10(4) and 5.3 × 10(5), respectively, were found in sample from the dump site. The presence of Sphingomonads, linA, and linB genes from HCH contaminated soil indicates the presence of indigenous bacterial communities capable of HCH degradation.

Functional screening of enzymes and bacteria for the dechlorination of hexachlorocyclohexane by a high-throughput colorimetric assay.

Two distinct microbial dehalogenases are involved in the first steps of degradation of hexachlorocyclohexane (HCH) isomers. The enzymes, LinA and LinB, catalyze dehydrochlorination and dechlorination reactions of HCH respectively, each with distinct isomer specificities. The two enzymes hold great promise for use in the bioremediation of HCH residues in contaminated soils, although their kinetics and isomer specificities are currently limiting. Here we report the functional screening of a library of 700 LinA and LinB clones generated from soil DNA for improved dechlorination activity by means of a high throughput colorimetric assay. The assay relies upon visual colour change of phenol red in an aqueous medium, due to the pH drop associated with the dechlorination reactions. The assay is performed in a microplate format using intact cells, making it quick and simple to perform and it has high sensitivity, dynamic range and reproducibility. The method has been validated with quantitative gas chromatographic analysis of promising clones, revealing some novel variants of both enzymes with superior HCH degrading activities. Some sphingomonad isolates with potentially superior activities were also identified.

Sphingopyxis indica sp. nov., isolated from a high dose point hexachlorocyclohexane (HCH)-contaminated dumpsite.

A Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped and light-yellow-pigmented bacterium, designated DS15(T), was isolated from a soil sample collected from a hexachlorocyclohexane dumpsite in Lucknow, Uttar Pradesh, India. Strain DS15(T) showed highest 16S rRNA gene sequence similarity to Sphingopyxis panaciterrulae DCY34(T) (98.7%) and Sphingopyxis soli BL03(T) (98.0%). The 16S rRNA gene sequence similarity between strain DS15(T) and species of genus Sphingopyxis with validly published names ranged from 92.5% to 98.7%. The DNA G+C content of strain DS15(T) was 67.5 mol%. The chemotaxonomic markers in strain DS15(T) were consistent with its classification in the genus Sphingopyxis, i.e. Q-10 as the major ubiquinone and summed feature 8 (C18:1ω7c/C18:1ω9c), C17:1ω6c, summed feature 3 (C16:1ω7c/C16:1ω6c), C14:0 2-OH, C15:0 2-OH, C16:0 and C17:1ω8c as the predominant fatty acids. The major polar lipids of strain DS15(T) were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylcholine (PC), phosphatidylglycerol (PG) and sphingoglycolipids (SGL) and spermidine was detected as the major polyamine. Phylogenetic analysis, DNA-DNA hybridization, and chemotaxonomic and phenotypic analysis support the conclusion that strain DS15(T) represents a novel species within the genus Sphingopyxis, for which the name Sphingopyxis indica is proposed. The type strain is DS15(T) (=MTCC 9455(T)=CCM 7542(T)=MCC 2023(T)).

Contrastive Representation Learning for Gaze Estimation.

Self-supervised learning (SSL) has become prevalent for learning representations in computer vision. Notably, SSL exploits contrastive learning to encourage visual representations to be invariant under various image transformations. The task of gaze estimation, on the other hand, demands not just invariance to various appearances but also equivariance to the geometric transformations. In this work, we propose a simple contrastive representation learning framework for gaze estimation, named Gaze Contrastive Learning (GazeCLR). GazeCLR exploits multi-view data to promote equivariance and relies on selected data augmentation techniques that do not alter gaze directions for invariance learning. Our experiments demonstrate the effectiveness of GazeCLR for several settings of the gaze estimation task. Particularly, our results show that GazeCLR improves the performance of cross-domain gaze estimation and yields as high as 17.2% relative improvement. Moreover, the GazeCLR framework is competitive with state-of-the-art representation learning methods for few-shot evaluation. The code and pre-trained models are available at https://github.com/jswati31/gazeclr.

Assessment of residual gastric volume using point-of-care ultrasonography in adult patients who underwent elective surgery.

BACKGROUND: Aspiration pneumonitis remains a dreaded complication that may lead to almost 9% of anaesthesia-related deaths. The presence of gastric contents has always been a contributing factor to an increased risk of aspiration. Preoperative gastric ultrasound has been suggested as a modality for determining residual volume in special populations and conditions. We conducted an observational study to determine the gastric residual volume in preoperative patients of elective surgery with gastric ultrasound and to study its correlation with patient factors. METHODS: We enrolled 411 patients in the age group of 18-80 with ASA-PS I and II having BMI less than 35 kg/m2. Patients with prior gastrointestinal surgery and parturients were excluded from the present study. Gastric antrum in both supine and right lateral decubitus positions was measured using USG in the immediate preoperative period, and gastric residual volume was calculated, which was subsequently correlated with various patient factors. RESULTS: On qualitative assessment, 97 and 118 patients were observed to have distended stomachs in the supine and right lateral decubitus positions, respectively. On quantitative assessment, 336 had safe GRV, 60 patients were classified as having a low risk of aspiration (GRV < 1.5 ml/kg) while 13 had a high risk of aspiration (> 1.5 ml/kg). Eight patients with a fasting duration of more than ten hours and five who fasted between 6 and 10 h had a gastric residual volume of more than 1.5 ml/h. Patients who were premedicated with histamine blockers had a statistically significant higher antral cross-sectional area (p-value - 0.022*) and GRV (p-value - 0.018*) in the right lateral decubitus position compared to patients who had taken proton pump inhibitors (PPIs). As BMI increased, there was a statistically significant (p-value < 0.001) increase in mean antral CSA in both supine and right lateral decubitus positions. There was a statistically significant association found between type 2 diabetes (p-value 0.045*) with antral grade. DISCUSSION: Patients can have significant residual volume (> 1.5 ml/kg) despite adequate fasting, and preoperative gastric ultrasound can help in assessing the same and guiding perioperative airway management. PPIs are more effective in reducing gastric residual volume as compared to histamine blockers. Patients with a BMI of more than 30 and type 2 diabetes mellitus have significant correlation with increased gastric residual volume mandating preoperative gastric ultrasound assessment for effective management. CONCLUSIONS: Patients with BMI over 30 and type 2 diabetes may benefit from POCGUS to guide perioperative airway management by stratifying GRV. Trial registration Name of registry-Clinical Trial Registry of India. Trial registration number-2020/03/024083. Date of registration-19.3.2020. URL- http://ctri.nic.in/Clinicaltrials/pmaindet2.php?trialid=39961&EncHid=&userName=.

Sphingomonas indica sp. nov., isolated from hexachlorocyclohexane (HCH)-contaminated soil.

A bacterial strain, designated Dd16(T), was isolated from a hexachlorocyclohexane (HCH) dumpsite at Lucknow, India. Cells of strain Dd16(T) were Gram-stain-negative, non-motile, rod-shaped and yellow-pigmented. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Sphingomonas in the family Sphingomonadaceae, as it showed highest sequence similarity to Sphingomonas asaccharolytica IFO 15499(T) (95.36 %), Sphingosinicella vermicomposti YC7378(T) (95.30), 'Sphingomonas humi' PB323 (95.20 %), Sphingomonas sanxanigenens NX02(T) (95.14%) and Sphingomonas desiccabilis CP1D(T) (95.00%). The major fatty acids were summed feature 3 (C(16:1)ω7c/C(16:1)ω6c) C(14:0) 2-OH, summed feature 8 (C(18:1)ω7c and/or C(18:1)ω6c) and C(16:0). The polar lipid profile of strain Dd16(T) also corresponded to those reported for species of the genus Sphingomonas (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, and a sphingoglycolipid), again supporting its identification as a member of the genus Sphingomonas. The predominant respiratory quinone was ubiquinone Q(10), and sym-homospermidine was the major polyamine observed. The total DNA G+C content of strain Dd16(T) was 65.8 mol%. The results obtained on the basis of phenotypic characteristics and phylogenetic analysis and after biochemical and physiological tests, clearly distinguished strain Dd16(T) from closely related members of the genus Sphingomonas. Thus, strain Dd16(T) represents a novel species of the genus Sphingomonas for which the name Sphingomonas indica sp. nov. is proposed. The type strain is Dd16(T) ( = DSM 25434(T)  = CCM 7882(T)).

Acinetobacter indicus sp. nov., isolated from a hexachlorocyclohexane dump site.

The taxonomic position of a Gram-negative, non-motile, oxidase negative and catalase positive strain, A648(T), isolated from a hexachlorocyclohexane (HCH) dump site located in Lucknow, India, was ascertained by using a polyphasic approach. A comparative analysis of a partial sequence of the rpoB gene and the 16S rRNA gene sequence revealed that strain A648(T) belonged to the genus Acinetobacter. DNA-DNA relatedness values between strain A648(T) and other closely related members (16S rRNA gene sequence similarity greater than 97%), namely Acinetobacter radioresistens DSM 6976(T), A. venetianus ATCC 31012(T), A. baumannii LMG 1041(T), A. parvus LMG 21765(T) A. junii LMG 998(T) and A. soli JCM 15062(T), were found to be less than 8%. The major cellular fatty acids of strain A648(T) were 18:1ω9c (19.6%), summed feature 3 (15.9%), 16:0 (10.6%) and 12:0 (6.4%). The DNA G+C content was 40.4 mol%. The polar lipid profile of strain A648(T) indicated the presence of diphosphatidylglycerol, phosphatidylethanolamine, followed by phosphatidylglycerol and phosphatidylcholine. The predominant polyamine of strain A648(T) was 1,3-diaminopropane and moderate amounts of putrescine, spermidine and spermine were also detected. The respiratory quinone consisted of ubiquinone with nine isoprene units (Q-9). On the basis of DNA-DNA hybridization, phenotypic characteristics and chemotaxonomic and phylogenetic comparisons with other members of the genus Acinetobacter, strain A648(T) is found to be a novel species of the genus Acinetobacter, for which the name Acinetobacter indicus sp. nov. is proposed. The type strain is A648(T) ( = DSM 25388(T) = CCM 7832(T)).

Tracker/Camera Calibration for Accurate Automatic Gaze Annotation of Images and Videos.

Modern appearance-based gaze tracking algorithms require vast amounts of training data, with images of a viewer annotated with "ground truth" gaze direction. The standard approach to obtain gaze annotations is to ask subjects to fixate at specific known locations, then use a head model to determine the location of "origin of gaze". We propose using an IR gaze tracker to generate gaze annotations in natural settings that do not require the fixation of target points. This requires prior geometric calibration of the IR gaze tracker with the camera, such that the data produced by the IR tracker can be expressed in the camera's reference frame. This contribution introduces a simple tracker/camera calibration procedure based on the PnP algorithm and demonstrates its use to obtain a full characterization of gaze direction that can be used for ground truth annotation.

Rethinking Model-Based Gaze Estimation.

Over the past several years, a number of data-driven gaze tracking algorithms have been proposed, which have been shown to outperform classic model-based methods in terms of gaze direction accuracy. These algorithms leverage the recent development of sophisticated CNN architectures, as well as the availability of large gaze datasets captured under various conditions. One shortcoming of black-box, end-to-end methods, though, is that any unexpected behaviors are difficult to explain. In addition, there is always the risk that a system trained with a certain dataset may not perform well when tested on data from a different source (the "domain gap" problem.) In this work, we propose a novel method to embed eye geometry information in an end-to-end gaze estimation network by means of a "geometric layer". Our experimental results show that our system outperforms other state-of-the-art methods in cross-dataset evaluation, while producing competitive performance over within dataset tests. In addition, the proposed system is able to extrapolate gaze angles outside the range of those considered in the training data.

A real time face mask detection system using convolutional neural network.

In current times, after the rapid expansion and spread of the COVID-19 outbreak globally, people have experienced severe disruption to their daily lives. One idea to manage the outbreak is to enforce people wear a face mask in public places. Therefore, automated and efficient face detection methods are essential for such enforcement. In this paper, a face mask detection model for static and real time videos has been presented which classifies the images as "with mask" and "without mask". The model is trained and evaluated using the Kaggle data-set. The gathered data-set comprises approximately about 4,000 pictures and attained a performance accuracy rate of 98%. The proposed model is computationally efficient and precise as compared to DenseNet-121, MobileNet-V2, VGG-19, and Inception-V3. This work can be utilized as a digitized scanning tool in schools, hospitals, banks, and airports, and many other public or commercial locations.