Assortment of Native Microalgae for Improved Biomass and Lipid Production on Employing Vegetable Waste as a Frugal Cultivation Approach for Biodiesel Application.

Enhanced biofuel production strategies from microalgae by employing affordable bio-waste usage are fetching significance, nowadays. This study examines the effect of VWE for enhanced biomass from new indigenous microalgal isolates, Asterarcys sp. SPC, Scenedesmus sp. KT-U, Scenedesmus sp. KTWL-A, Coelastrum sp. T-E, and Chlorella sp. TWL-B. The growth of microalgae in VWE-treated growth media showed considerable increase (1.14-2.3 folds) than control medium (without VWE). Further, two effective native microalgae were selected based on growth in VWE treatment, biomass productivity, and TAG accumulation through statistical clustering analysis. Mixotrophic batch cultivation of Scenedesmus sp. KT-U and Asterarcys sp. SPC cultivated using VWE treatment in the optimum concentration had produced significant average increase in BP (1.8 and 1.4 folds, respectively) than control (without VWE). Whereas in the lipid production phase, there was a noticeable increase in lipid yield in VWE-treated cells of lipid phase (231.8 ± 17.9 mg/L and 243.5 ± 25 mg/L) in Scenedesmus sp. KT-U and Asterarcys sp. SPC, respectively, than in control (140.5 ± 28 mg/L and 166.4 ± 23 mg/L) with considerable TAG accumulation. Thus, this study imparts strain selection process of native microalgae based on vegetable waste usage for improved yield of biomass and lipid amenable for cost-effective biodiesel production.

A cheap two-step cultivation of Phaeodactylum tricornutum for increased TAG production and differential expression of TAG biosynthesis associated genes.

A cheap cultivation of microalgae greatly reduces the biodiesel production cost. Subsequently in this study, citric acid and effluents from sugar and tannery industries were used as the nutritional supplements for the improvement of biomass and TAG production in Phaeodactylum tricornutum using two-step cultivation. When compared to control (media without supplementation), a considerable increase in biomass and chlorophyll a was obtained with citric acid (CA) and sugar industry effluent (SIE) supplemented media. In the two-step cultivation method, biomass raised from CA (100 mg·L-1) and SIE (1.5 mL·L-1) supplementations in the first step, viz. biomass production (BP) step was allowed for lipid accumulation in the second step, viz. lipid production (LP) step, and thus yielded enhanced lipids of 11.5 ± 0.7 mg·L-1·day-1 and 13.5 ± 1.9 mg·L-1·day-1 respectively, with improved TAG synthesis. Further, differential expression analysis of TAG biosynthetic genes of P. tricornutum under single-step and two-step cultivation modes were performed, and the gene expression patterns were studied.

Review on integrated biofuel production from microalgal biomass through the outset of transesterification route: a cascade approach for sustainable bioenergy.

In recent years, microalgal feedstocks have gained immense potential for sustainable biofuel production. Thermochemical, biochemical conversions and transesterification processes are employed for biofuel production. Especially, the transesterification process of lipid molecules to fatty acid alkyl esters (FAAE) is being widely employed for biodiesel production. In the case of the extractive transesterification process, biodiesel is produced from the extracted microalgal oil. Whereas In-situ (reactive) transesterification allows the direct conversion of microalgae to biodiesel avoiding the sequential steps, which subsequently reduces the production cost. Though microalgae have the highest potential to be an alternate renewable feedstock, the minimization of biofuel production cost is still a challenge. The biorefinery approaches that rely on simple cascade processes involving cost-effective technologies are the need of an hour for sustainable bioenergy production using microalgae. At the same time, combining the biorefineries for both (i) high value-low volume (food and health supplements) and (ii) low value- high volume (waste remediation, bioenergy) from microalgae involves regulatory and technical problems. Waste-remediation and algal biorefinery were extensively reviewed in many previous reports. On the other hand, this review focuses on the cascade processes for efficient utilization of microalgae for integrated bioenergy production through the transesterification. Microalgal biomass remnants after the transesterification process, comprising carbohydrates as a major component (process flow A) or the carbohydrate fraction after bio-separation of pretreated microalgae (process flow B) can be utilized for bioethanol production. Therefore, this review concentrates on the cascade flow of integrated bioprocessing methods for biodiesel and bioethanol production through the transesterification and biochemical routes. The review also sheds light on the recent combinatorial approaches of transesterification of microalgae. The applicability of spent microalgal biomass residue for biogas and other applications to bring about zero-waste residue are discussed. Furthermore, techno-economic analysis (TEA), life cycle assessment (LCA) and challenges of microalgal biorefineries are discussed.

Improved biomass and lipid production in Synechocystis sp. NN using industrial wastes and nano-catalyst coupled transesterification for biodiesel production.

In this study, the improved biomass (1.6 folds) and lipid (1.3 folds) productivities in Synechocystis sp. NN using agro-industrial wastes supplementation through hybrid response surface methodology-genetic algorithm (RSM-GA) for cost-effective methodologies for biodiesel production was achieved. Besides, efficient harvesting in Synechocystis sp. NN was achieved by electroflocculation (flocculation efficiency 97.8±1.2%) in 10min when compared to other methods. Furthermore, different pretreatment methods were employed for lipid extraction and maximum lipid content of 19.3±0.2% by Synechocystis sp. NN was attained by ultrasonication than microwave and liquid nitrogen assisted pretreatment methods. The highest FAME (fatty acid methyl ester) conversion of 36.5±8.3mg FAME/g biomass was obtained using titanium oxide as heterogeneous nano-catalyst coupled whole-cell transesterification based method. Conclusively, Synechocystis sp. NN may be used as a biodiesel feedstock and its fuel production can be enriched by hybrid RSM-GA and nano-catalyst technologies.

Enhanced Extracellular Polysaccharide Production and Self-Sustainable Electricity Generation for PAMFCs by Scenedesmus sp. SB1.

In this study, a freshwater microalga, Scenedesmus sp. SB1, was isolated, purified, and identified by its internal transcribed spacer region (ITS1-5.8S-ITS2). Media optimization through the Plackett-Burman Design and response surface methodology (RSM) showed a maximum exopolysaccharide (EPS) production of 48 mg/L (1.8-fold higher than that for unoptimized media). Characterization using gas chromatography-mass spectrometry, Fourier transform infrared, X-ray diffraction, and thermogravimetric analysis reveals that the EPS is a sulfated pectin polysaccharide with a crystallinity index of 15.2% and prompt thermal stability. Furthermore, the photoelectrogenic activity of Scenedesmus sp. SB1 inoculated in BG-11 and RSM-optimized BG-11 (ROBG-11) media was tested by cyclic voltammogram studies, revealing the potential of the inoculated strain in ROBG-11 toward photosynthetic algal microbial fuel cells over normal BG-11. To the best of our knowledge, functional group characterization, physical and thermal property and media optimization for EPS production by RSM and electrogenic activity studies are reported for the first time in Scenedesmus sp. SB1.

Characterization and fatty acid profiling in two fresh water microalgae for biodiesel production: Lipid enhancement methods and media optimization using response surface methodology.

Two fresh water microalgae, Coelastrella sp. M-60 and Micractinium sp. M-13 were investigated in this study for their potential of biodiesel production. For increasing biomass and lipid production, these microalgae were subjected to nutrient starvation (nitrogen, phosphorous, iron), salinity stress and nutrient supplementation with sugarcane industry effluent, citric acid, glucose and vitamin B12. The lipid productivity obtained from the isolates Coelastrella sp. M-60 (13.9 ± 0.4 mg/L/day) and Micractinium sp. M-13 (11.1 ± 0.2 mg/L/day) was maximum in salinity stress. The media supplemented with all the four nutrients yielded higher lipid productivity than the control. The response surface methodology (RSM) was employed to evaluate the effect of sugarcane industry effluent and citric acid on growth and lipid yield. Fatty acid profile of Coelastrella sp. M-60 and Micractinium sp. M-13 were composed of C-14, C-16:0, C-18:0, C-18:1 and C-18:2 and their fuel properties were also in accordance with international standards.