RESUMO
An SRV-like virus was isolated from a colony-born Japanese monkey. To identify this SRV-like virus, we designed universal primers at regions that were conserved among the reported SRV sequences in the 5'-LTR and the short ORF and we obtained plasmid clones containing the complete gag, prt, pol and env genes. The full-length sequences of the isolate were determined from the plasmids and by direct sequencing. Sequence comparisons and phylogenetic analyses indicated that this SRV-like virus had a sequence identical to the reported 626 bp of SRV-5. In this study, we isolated SRV5/JPN/2005/V1 from a Japanese monkey and characterized the full-length SRV-5 sequence.
Assuntos
Genes Virais/genética , Genoma Viral/genética , Macaca , Doenças dos Macacos/virologia , Infecções por Retroviridae/virologia , Retrovirus dos Símios/isolamento & purificação , Sequência de Aminoácidos , Animais , Sequência de Bases , Primers do DNA/genética , DNA Viral/genética , Genes env/genética , Genes gag/genética , Genes pol/genética , Japão , Dados de Sequência Molecular , Filogenia , Retrovirus dos Símios/classificação , Retrovirus dos Símios/genética , Análise de Sequência de DNAAssuntos
Antagonistas Colinérgicos/farmacologia , Clozapina/efeitos adversos , Esquizofrenia Paranoide/tratamento farmacológico , Escopolamina/farmacologia , Sialorreia/induzido quimicamente , Adulto , Antagonistas Colinérgicos/administração & dosagem , Feminino , Humanos , Pomadas , Escopolamina/administração & dosagemRESUMO
Two simian Entamoeba histolytica-like strains, EHMfas1 and P19-061405, have been suggested to represent a new species based on genetic characterization. Sequence analyses of the hexokinase, glucose phosphate isomerase, and phosphoglucomutase genes supported the previous findings of isoenzyme analyses demonstrating a new zymodeme pattern. Phylogenetic studies of 18S rDNA, 5.8S rDNA, the chaperonin 60 gene, and the pyridine nucleotide transhydrogenase gene showed original clusters of simian E. histolytica-like strains below or near E. histolytica, respectively. Comparative studies of the chitinase and the serine-rich E. histolytica protein genes and locus 1-2 region revealed that most mutated units were shared among the simian E. histolytica-like strains. The similarities of each of the repeating units within the simian E. histolytica-like strains or E. histolytica and the differences of those between the both might be generated by concerted evolution. Our results indicate that EHMfas1 and P19-061405 should be considered to be the same species, despite that they were isolated from different monkey species and different habitats. Simian E. histolytica-like amebas may be endemic to macaque monkeys, as a counterpart to E. histolytica in humans, and should be differentiated from E. histolytica by the revival name Entamoeba nuttalli, as proposed for P19-061405.