Community structure of a sulfate-reducing consortium in lead-contaminated wastewater treatment process.

This study evaluated the capacity to remove lead by an indigenous consortium of five sulfate-reducing bacteria (SRB): Desulfobacterium autotrophicum, Desulfomicrobium salsugmis, Desulfomicrobium escambiense, Desulfovibrio vulgaris, and Desulfovibrio carbinolicus, using continuous moving bed biofilm reactor systems. Four continuous moving bed biofilm reactors (referred as R1-R4) were run in parallel for 40 days at lead loading rates of 0, 20, 30 and 40 mg l-1 day-1, respectively. The impact of lead on community structure of the SRB consortium was investigated by dsrB gene-based denaturing gradient gel electrophoresis (dsrB-based DGGE), fluorescence in situ hybridization (FISH) and chemical analysis. These results indicated that D. escambiense and D. carbinolicus were dominant in all analyzed samples and played a key role in lead removal in R2 (20 mg l-1 day-1) and R3 (30 mg l-1 day-1). However, in R4 (40 mg l-1 day-1), these two strains were barely detected by FISH and dsrB-based DGGE. As a result, SRB activity was severely affected by lead toxicity. High lead removal efficiencies of lead (99-100%) were observed in R2 and R3 throughout the operation, whereas that in R4 was significantly decreased (91%) after 40 days of operation. This data strongly implied that the investigated SRB consortium might have potential application for lead removal. Moreover, to improve the efficiency of the lead treatment process, the lead loading rates below the inhibitory level to SRB activity should be selected.

The effect of heavy metals on microbial community structure of a sulfidogenic consortium in anaerobic semi-continuous stirred tank reactors.

The effect of heavy metals on community structure of a heavy metal tolerant sulfidogenic consortium was evaluated by using a combination of denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene and dissimilatory sulfite reductase (dsrB) gene fragments, 16S rRNA gene cloning analysis and fluorescence in situ hybridization (FISH). For this purpose, four anaerobic semi-continuous stirred tank reactors (referred as R1-R4) were run in parallel for 12 weeks at heavy metal loading rates of 1.5, 3, 4.5 and 7.5 mg l(-1) d(-1) each of Cu(2+), Ni(2+), Zn(2+), and Cr(6+), respectively. The abundance ratio of Desulfovibrio vulgaris detected by FISH to total cell counts was consistent with the obtained results of cloning and DGGE. This indicated that D. vulgaris was dominant in all analyzed samples and played a key role in heavy metal removal in R1, R2, and R3. In contrast, after 4 weeks of operation of R4, a distinct biomass loss was observed and no positive hybridized cells were detected by specific probes for the domain Bacteria, sulfate-reducing bacteria and D. vulgris. High removal efficiencies of heavy metals were achieved in R1, R2 and R3 after 12 weeks, whereas the precipitation of heavy metals in R4 was significantly decreased after 4 weeks and almost not observed after 6 weeks of operation. In addition, the anaerobic bacteria, such as Pertrimonas sulfuriphila, Clostridium sp., Citrobacter amalonaticus, and Klebsiella sp., identified from DGGE bands and clone library were hypothesized as heavy metal resistant bacteria at a loading rate of 1.5 mg l(-1) d(-1) of Cu(2+), Ni(2+), Zn(2+), and Cr(6+.)

Heavy metal removal in anaerobic semi-continuous stirred tank reactors by a consortium of sulfate-reducing bacteria.

Removal of heavy metals by an enriched consortium of sulfate-reducing bacteria (SRB) was evaluated through the abundance of SRB, sulfate reduction, sulfide production and heavy metal precipitation. Five parallel anaerobic semi-continuous stirred tank reactors (CSTR, V = 2 L) (referred as R1-R5) were fed with synthetic wastewater containing mixtures of Cu(2+), Zn(2+), Ni(2+), and Cr(6+) in the concentrations of 30, 60, 90, 120, and 150 mg L(-1) of each metal and operated with a hydraulic retention time of 20 days for 12 weeks. The loading rates of each metal in R1-R5 were 1.5, 3, 4.5, 6, and 7.5 mg L(-1) d(-1), respectively. The results showed that there was no inhibition of SRB growth and that heavy metal removal efficiencies of 94-100% for Cu(2+), Zn(2+), Ni(2+), and Cr(6+) were achieved in R1-R3 throughout the experiment and in R4 during the first 8 weeks. The toxic effect of heavy metals on the SRB consortium was revealed in R5, in which no SRB could survive and almost no heavy metal precipitation was detected after four weeks of operation.