Switching Electric Double Layer Potential by Phase Structure Control for Advanced Oxygen Reduction Reaction of Cobalt@Nitrogen Doped Carbon Core-Shell.

The key to design an advanced oxygen reduction reaction (ORR) electrocatalyst is a well-balance between the adsorption and desorption of oxygen intermediates. This study systematically evaluated the ORR activity of HCP and FCC cobalt core-shell cobalt/N-doped carbon (Cobalt@NC) catalyst via theoretical and experimental studies. The electronic structure calculations using density functional theory (DFT) calculations revealed that the ORR activity of carbon layer can be improved by 1) switching the electrostatic potential in the electrical double layer due to the polarization induced at the carbon-cobalt interface and 2) modulating the electron population in the bonding orbital in the C-O bonds in an ORR. The results revealed that an O atom is bounded stronger to the outer NC shell with FCC Cobalt than HCP Cobalt, which hindered the desorption steps of OH*. Experimentally, plasma-engineered HCP Cobalt@NC also showed remarkably advanced performance toward ORR compared to that FCC Cobalt@NC. The kinetic current density of HCP Cobalt@NC at 0.85 V versus RHE is calculated as 6.24 mA cm-2, which is six folds higher than FCC Cobalt@NC and even outperform 20 wt.% Pt/C. In a practical Aluminium-air battery, HCP Cobalt@NC also exhibited slightly higher peak power density (110.57 mW cm-2) compared to 20 wt.% Pt/C.

Responsiveness and Minimal Clinically Important Difference of the Canadian Occupational Performance Measure Among Patients With Frozen Shoulder.

IMPORTANCE: Patient-reported outcome measures provide insights into intervention effects on patients. The Canadian Occupational Performance Measure (COPM) emphasizes identifying priorities in daily activity engagement and evaluating an individual's perception of changes over time. OBJECTIVE: To assess the responsiveness of the COPM and the minimal clinically important difference (MCID) among patients with frozen shoulders. DESIGN: Prospective, single-blind, randomized controlled trial. SETTING: Two physical medicine and rehabilitation clinics. PARTICIPANTS: Ninety-four patients with frozen shoulders enrolled in a previous study. OUTCOMES AND MEASURES: Baseline and 3-mo evaluations of the COPM and other measures. Responsiveness was assessed using effect size (ES) and standardized response mean (SRM). The MCID values were determined through a distribution-based approach, which used the 0.5 standard deviation and ES methods, and an anchor-based approach, which used the receiver operating characteristic curve method. RESULTS: The ES and SRM results indicated that the COPM had high responsiveness. The distribution-based MCID values for COPM Performance and COPM Satisfaction were 1.17 and 1.44, respectively. The anchor-based MCID values were 2.5 (area under the curve [AUC] = 0.78, 95% confidence interval [CI] [0.64-0.91]) and 2.1 (AUC = 0.76, 95% CI [0.60-0.91]), respectively. CONCLUSIONS AND RELEVANCE: The findings suggest that the COPM is a responsive outcome measure for patients with frozen shoulder. The established MCID values for the COPM can be valuable for interpreting changes in patient performance and satisfaction, thus aiding clinical interventions and research planning. Plain-Language Summary: This is the first study to review the effectiveness of the Canadian Occupational Performance Measure (COPM) to determine the success of occupational therapy interventions for people with a frozen shoulder. The findings suggest that the COPM is an effective and valuable tool for clients with a frozen shoulder to understand their experiences and treatment priorities and to detect meaningful changes in their performance and satisfaction after an occupational therapy intervention.

Associations of Circulating Levels of Sphingosine 1-Phosphate with the Trabecular Bone Score and Bone Mineral Density in Postmenopausal Women.

Despite the potential roles of sphingosine 1-phosphate (S1P) as a biomarker of osteoporotic fracture (OF), independent of bone mineral density (BMD) and clinical risk factors (CRFs), its association with bone microarchitecture, a key determinant of bone quality, have not been studied yet. We here investigated the association of S1P with the trabecular bone score (TBS), an index of the bone microarchitecture. The plasma S1P concentrations, TBS, and BMD were measured in the 339 postmenopausal women. The S1P level was inversely correlated with the TBS (γ=-0.096, p=0.049) and BMD at the femur neck (FN-BMD: γ=-0.122, p=0.025) and tended to be inversely correlated the BMD at the total hip (TH-BMD: γ=-0.096, p=0.079), but not at the lumbar spine (LS-BMD). After adjusting for fracture risk assessment tool probabilities of major OF from CRFs, the S1P level was inversely associated with the TBS (ß=-0.096, p=0.049) and FN-BMD (ß=-0.118, p=0.025) and tended to be inversely associated with the TH-BMD (ß=-0.092, p=0.083). Compared with subjects in the lowest S1P tertile, those in the highest S1P tertile had a significantly lower TBS (p=0.032) and BMD at femur (p=0.004-0.036). These findings indicated that a high S1P level in postmenopausal women was inversely associated with the both bone mass and microarchitecture, reflecting the compromised bone strength.

High Circulating Sphingosine 1-Phosphate is a Risk Factor for Osteoporotic Fracture Independent of Fracture Risk Assessment Tool.

Circulating sphingosine 1-phosphate (S1P) levels may be a biomarker for osteoporotic fracture (OF). This study assessed whether the addition of S1P levels to the fracture risk assessment tool (FRAX) could improve predictability of OF risk. Plasma S1P concentrations and FRAX variables were measured in 81 subjects with and 341 subjects without OF. S1P levels were higher in subjects with than those without OF (3.11 ± 0.13 µmol/L vs. 2.65 ± 0.61 µmol/L, P = 0.001). Higher S1P levels were associated with a higher likelihood of OF (odds ratio [OR] = 1.33, 95% confidence interval [CI] = 1.05-1.68), even after adjusting for FRAX probabilities. Compared with the lowest S1P tertile, subjects in the middle (OR = 3.37, 95% CI = 1.58-7.22) and highest (OR = 3.65, 95% CI = 1.66-8.03) S1P tertiles had higher rates of OF after adjustment. The addition of S1P levels to FRAX probabilities improved the area under the receiver-operating characteristics curve (AUC) for OF, from 0.708 to 0.769 (P = 0.013), as well as enhancing category-free net reclassification improvement (NRI = 0.504, 95% CI = 0.271-0.737, P < 0.001) and integrated discrimination improvement (IDI = 0.044, 95% CI = 0.022-0.065, P < 0.001). Adding S1P levels to FRAX probabilities especially in 222 subjects with osteopenia having a FRAX probability of 3.66-20.0% markedly improved the AUC for OF from 0.630 to 0.741 (P = 0.012), as well as significantly enhancing category-free NRI (0.571, 95% CI = 0.221-0.922, P = 0.001) and IDI (0.060, 95% CI = 0.023-0.097, P = 0.002). S1P is a consistent and significant risk factor of OF independent of FRAX, especially in subjects with osteopenia and low FRAX probability.

Genetic characterization of porcine epidemic diarrhea virus in Korea from 1998 to 2013.

The number of porcine epidemic diarrhea (PED) cases has increased over the past 20 years in Korea, with a major outbreak in 2013. A total of 27 Korean strains from 1998 to 2013 were analyzed (excluding the noncoding regions) and divided into two groups for comparison of the spike (S), ORF3, envelope (E), membrane (M), and nucleocapsid (N) genes with those of reference strains, vaccine strains, and previously identified strains based on phylogenetic analysis. Analysis of the selection patterns of PEDV isolated in Korea indicated positive selection of nine nonsynonymous sites in the S and N proteins and negative selection at 97 sites for all of the proteins. Interestingly, eight nonsynonymous mutations in S showed no significant pattern change over the 15-year period, and one of eight mutation sites was found only in IC05TK, GN05DJ, and KNU0802 in the epidemic years 2005 and 2008. These eight mutations were also present during the epidemic years in China. Furthermore, of the signs of positive selection in the S protein, the conservative substitutions were more frequent than radical substitutions in PEDVs, suggesting that the evolution of Korean strains has been slow. Serological cross-reactivity was detected between three field PEDVs and two vaccine strains, with different serum neutralization titers. In conclusion, although Korean PEDVs have been evolving slowly, their diverse antigenicity and genetics imply that multilateral efforts to prevent future PED outbreaks are required.

A novel reassortant H1N2 virus related to the pandemic H1N1 2009 influenza virus isolated from Korean pigs.

Since the discovery of the pandemic H1N1 (pH1N1) virus in 2009, a novel reassortant H1N2 virus (A/Swine/Korea/VDS1/2010) containing the pH1N1 segments has been detected in Korean pig populations. The hemagglutinin and neuraminidase genes of this virus are derived from reassortant H1N1- and H1N2-group viruses, respectively, identified in Korean pigs, while other genes originate from contemporary circulating pH1N1 viruses. The antigenic and biological properties of this novel virus, as determined by clinical, pathological, serological, and genetic analyses, are similar to those of pH1N1 viruses, which infect swine easily (Weingartl et al. J Virol 84:2245-2256, 2010; Brookes et al. PLoS one 5:e9068, 2010; Lange et al. J Gen Virol 90:2119-2123, 2009). Determining whether this virus will become established and pose a threat to mammalian populations requires further investigation.

Serological characteristics of affected cattle during an outbreak of bovine enzootic encephalomyelitis caused by Akabane virus.

During an outbreak of bovine enzootic encephalomyelitis caused by the Akabane virus (AKAV) in 2010, 210 serum samples were collected from the affected cattle, and serological investigations for the AKAV were performed using a serum neutralization test (SNT) and an enzyme-linked immunosorbent assay (ELISA). The seropositive rates for SNT and ELISA were 90.0 and 85.2 %, respectively. The titers of SNT (log2) against the AKAV were higher than 4.0 in the highly affected cattle (80.0 %). This finding indicates that most affected cattle were infected with the AKAV and that strong immune responses against this virus were elicited in affected cattle. The strong immune response to the AKAV in cattle may provide insight into the occurrence of bovine encephalomyelitis caused by the AKAV.

Canine adenovirus type 1 in a fennec fox (Vulpes zerda).

A 10-mo-old female fennec fox (Vulpes zerda) with drooling suddenly died and was examined postmortem. Histologic examination of different tissue samples was performed. Vacuolar degeneration and diffuse fatty change were observed in the liver. Several diagnostic methods were used to screen for canine parvovirus, canine distemper virus, canine influenza virus, canine coronavirus, canine parainfluenza virus, and canine adenovirus (CAdV). Only CAdV type 1 (CAdV-1) was detected in several organs (liver, lung, brain, kidney, spleen, and heart), and other viruses were not found. CAdV-1 was confirmed by virus isolation and nucleotide sequencing.

Parental control and college students' depressive symptoms: A latent class analysis.

We examined how maternal and paternal parental control (helicopter parenting, behavioral control, and psychological control) among college students are related to their depressive symptoms. We collected data from college students who attended a private university in Upstate New York (n = 455) and analyzed it using three-step latent class analysis. Latent class analysis identified four parental control latent classes: weak parental control, strong psychological control, strong helicopter parenting, and strong parental control. College students in the weak parental control class reported lower depressive symptoms than those in strong psychological control and strong parental control classes. In addition, college students in the strong helicopter parenting class reported lower depressive symptoms than those in strong psychological control and strong parental control classes. College students' depressive symptoms were not significantly different between weak parental control and strong helicopter parenting classes. Future researchers are encouraged to continue to acknowledge the characteristics of different forms of parental control and their influence on college students' quality of life in the transition to adulthood, considering the unique approaches of both parents.

Isolation of influenza A(H3N2)v virus from pigs and characterization of its biological properties in pigs and mice.

Recently, a novel reassortant virus, influenza A(H3N2)v [A(H3N2)v], was identified as the causative pathogen in 307 human cases of influenza in the United States. A(H3N2)v contains the matrix gene from the 2009 pandemic H1N1 (pH1N1) virus, while its other genes originate from H3N2 viruses with triple-reassorted internal genes. In this study, we isolated three A(H3N2)v viruses from commercial pigs in Korea that showed similarities with published human A(H3N2)v viruses in eight segment sequence alignments. After genetic characterization, the pathogenicity of one of these viruses was assessed in pigs and mice. Infection of pigs with this novel virus resulted in mild interstitial pneumonia with marked oronasal shedding of viral RNA for about 14 days. In mice, the virus replicated efficiently in the lungs; viral RNA was detected up to 9 days post-inoculation. However, the virus did not cause severe disease or death in mice, despite the administration of a high infectious dose (10(5.2) TCID50). This study demonstrates that A(H3N2)v causes a high morbidity rate with low virulence; however, global monitoring of A(H3N2)v outbreaks in mammals will be needed to determine whether this novel subtype will shift to a highly pathogenic virus.

Complete genome sequences of three rabies viruses isolated from rabid raccoon dogs and a cow in Korea.

The complete genomes of three rabies viruses (BD0406CC, BV9901PJ, and 08F40) of two raccoon dogs (Nyctereutes procyonoides koreensis) and a cow were determined. The genomic organization is typical of rabies viruses, and the open reading frames of the N, P, M, G, and L genes are 1,353, 894, 609, 1,575, and 6,384 bases in length, respectively. The full genome length of the three strains was 11,928 nucleotides, and the sequence similarity between the rabies viruses at the nucleotide level was 98.5-99.5%. Sequence comparisons indicated that these rabies viruses belong to the "Arctic and Arctic-like" group, with high homology to the Eurasian cluster. All Korean strains were clustered with the Mongolia strains, which belong to Arctic-like 1 clade. The 08F40 and BD0406CC strains were constructed with rabies virus strains isolated in Gangwon province. The BV9901PJ strain was closely related to strains isolated in Gyeonggi province in Korea. Three strains were more dependent upon geographical distribution and time period than host species. Complete genome sequencing of different host-origin rabies viruses will provide information that should contribute to understanding the transmission cycle and genetic variability of rabies from different hosts.

Detection and genetic characterization of feline kobuviruses.

In order to survey for feline kobuviruses infection, fecal samples (n = 39) of cats with diarrhea were collected during 2011-2012. Six (14.5%) of the fecal samples tested were positive for feline kobuviruses. The partial nucleotide sequences of feline kobuviruses based on the RNA-dependent RNA polymerase gene were compared to those of other species. Feline kobuviruses were most closely related to canine kobuvirus in terms of their amino acid and nucleotide levels. In a phylogenetic tree, feline kobuviruses were also closely clustered with canine kobuvirus, Aichi virus (human), and mouse kobuvirus. This is the first report of the detection and genetic characterization of feline kobuviruses.

Can helicopter parenting be beneficial for parent-child relationships? A person-centered approach in the United States and South Korea.

Introduction: We aimed to uncover latent classes of maternal and paternal helicopter parenting among American and Korean college students and to examine whether latent classes of maternal and paternal helicopter parenting are associated with parent- child relationships. Methods: We conducted three-step latent class analyses using five helicopter parenting indicators for 433 mother-child and 401 father-child groups in the United States and 207 mother-child and 195 father-child groups in South Korea. Results: We identified the same three helicopter parenting latent classes (strong, weak, and managed) in mother-child and father-child groups in the United States and Korea. In addition, we found that American and Korean college students in strong and managed helicopter parenting latent classes reported better parent- child relationships than those in the weak class regardless of parents' gender. Discussion: Our findings indicate that helicopter parenting is multidimensional and can be similarly interpreted by college students in Western and Eastern societies. Further, our findings suggest that helicopter parenting could be helpful for college students to establish an intimate relationship with their parents.

A multidimensional construct of helicopter parenting and college students' game and social media addictive behaviors: A cross-cultural study in South Korea and China.

Introduction: We explored latent classes of helicopter parenting among Korean and Chinese college students. In addition, we examined whether these latent classes of helicopter parenting are related to Korean and Chinese students' game and social media addictive behaviors. Methods: A three-step latent class analysis was conducted using 452 students from six universities in South Korea and 372 students from four universities in China. Results and discussion: We identified four distinct helicopter parenting latent classes among the parents of Korean and Chinese students: weak, strong, academic management, and academic and schedule management. We also found that Korean students in the strong class reported significantly higher levels of game and social media addictive behaviors than those in the other three classes, but this did not hold for Chinese students. This finding indicates that the association between helicopter parenting and college students' game and social media addictive behaviors can be differentiated within Asian cultural contexts.

Home-Based Intermittent Pneumatic Compression Therapy: The Impact in Chronic Leg Lymphedema in Patients Treated for Gynecologic Cancer.

We conducted a prospective study of cancer patients to investigate the efficacy, quality of life, satisfaction, and safety of a home-based intermittent pneumatic compression (IPC) device during the maintenance phase of lower extremity lymphedema. This device has a unique mode designed to mimic the manual lymphatic drainage (MLD) technique and thereby gently facilitate lymphatic draining of proximal extremities. Thirty patients with stage 3 chronic secondary unilateral leg lymphedema in the maintenance phase underwent IPC and conventional compression therapy for 4 weeks at home. The participants were guided to use 1 h course (30 min of MLD-mimicking mode and 30 min of conventional mode) of IPC device twice a day for 4 weeks. We assessed the patients' limb-volume measurement, quality of life (QOL), and satisfaction four times. There were no significant time-dependent interactions in the inter-limb volume difference ratio (Vratio). In a subgroup analysis, participants who used the home-based IPC device and maintained their routine self-maintenance program of short-stretch bandages (group B, n = 21) showed a more significant decline in Vratio than those who did not maintained their routine care (group A, n = 9). All scores of QOL decreased significantly after the intervention without subgroup difference. All participants were satisfied with the 4-week intervention. This study demonstrated that a home-based IPC device with an MLD-mimicking program is a useful option for maintaining the volume of limbs and improving the QOL of patients with stage 3 chronic leg lymphedema during the maintenance phase.

Detection of a novel porcine circovirus 4 in Korean pig herds using a loop-mediated isothermal amplification assay.

A novel porcine circovirus 4 has been recently identified in China and Korea. A sensitive and specific diagnostic method is urgently required to detect the virus in field samples. We developed a loop-mediated isothermal amplification (LAMP) the assay for the visual detection of PCV4 and evaluated its sensitivity, specificity, and applicability in clinical samples. This assay's results can be directly visualized by the naked eye using hydroxynaphthol blue after incubation for 40 min at 64 °C. The assay specifically amplified PCV4 DNA and no other viral nucleic acids. The sensitivity of the assay was <50 DNA copies/reaction, which was 10 times more sensitive than conventional polymerase chain reaction (cPCR) and comparable to real-time PCR (qPCR). Clinical evaluation revealed that the PCV4 detection rate in individual pig samples and at the farm level was 39.3 % (57/145) and 45.7 % (32/70), respectively, which were higher than cPCR (46 samples, 24 farms) and qPCR (52 samples, 29 farms) results. Cumulatively, owing to the advantages of high sensitivity and specificity, direct visual monitoring of the results, no possibility for cross-contamination, and being a low-cost equipment, the developed LAMP assay will be a valuable tool for the detection of the novel PCV4 in clinical samples, even in resource-limited laboratories.

One-step multiplex reverse-transcriptase PCR for detecting pandemic (H1N1) 2009 influenza virus.

Pandemic (H1N1) 2009 influenza has spread throughout the world since April 2009 and has caused many human deaths since its first report in humans. Pandemic (H1N1) 2009 influenza virus was first identified in a Canadian pig herd in April 2009 and has been reported in more than ten countries, including Korea. We developed a one-step multiplex reverse transcriptase polymerase chain reaction (RT-PCR) assay based on the matrix gene that discriminates pandemic (H1N1) 2009 influenza virus from endemic swine influenza viruses. The sensitivity of this assay was 100 copies of in vitro-transcribed target RNA and 0.01 tissue culture infective dose (TCID(50)/ml) of virus and was as high as those of conventional influenza A virus common matrix reverse transcriptase PCR assays and real-time reverse transcriptase PCR assays (1 to 200 copies) developed for detecting pandemic (H1N1) 2009 influenza viruses from human and pig samples. This one-step multiplex RT-PCR assay would be a good tool in monitoring pandemic (H1N1) 2009 influenza virus among pig herds on a regular basis.

Enhancing ORR/OER active sites through lattice distortion of Fe-enriched FeNi3 intermetallic nanoparticles doped N-doped carbon for high-performance rechargeable Zn-air battery.

Low-cost, high-activity, non-precious metal electrocatalysts are needed to enhance the bifunctional oxygen activities of rechargeable Zn-Air batteries. In this study, a Fe-enriched FeNi3 inter-metallic nanoparticle/nitrogen-doped carbon (Fe-enriched-FeNi3/NC) electrocatalyst was designed and prepared using a facile method based on plasma engineering. The excess Fe-ions in the Fe-enriched FeNi3 nanoparticles led to a high degree of lattice distortion that produced abundant oxygen-active sites. The electrocatalyst exhibited excellent oxygen evolution reaction (OER) activity as well as favorable oxygen reduction reaction (ORR) activity in an alkaline electrolyte. In addition, the electrocatalyst revealed a lower potential difference (ΔE = 0.80 V vs. RHE) in a bifunctional oxygen reaction compared to that of the benchmark 20 wt% Pt/C + Ir/C (ΔE = 0.84 V vs. RHE), and most of the reported FeNi3 alloy-doped carbon catalysts. Based on DFT calculations, the lattice distortion in Fe-enriched-FeNi3/NC promoted a higher density of active electrons around the Fermi level. Owing to its great bifunctional oxygen activities, Fe-enriched FeNi3/NC was applied as an ORR/OER catalyst in the air cathode in a homemade zinc-air battery and exhibited an excellent discharge-charge voltage gap (0.89 V), peak power density (89 mW/cm2), and high specific capacity of 734 mAh/g at 20 mA/cm2, which outperformed the benchmark 20 wt% Pt/C + Ir/C electrocatalyst. In summary, this research provides a novel strategy to enhance the OER/ORR activities of transition metal-based alloys through lattice distortion defects. In addition, it provides a new pathway for achieving noble metal-free air cathode materials for the next generation Zn-air battery.

Ultrasonic Plasma Engineering Toward Facile Synthesis of Single-Atom M-N4/N-Doped Carbon (M = Fe, Co) as Superior Oxygen Electrocatalyst in Rechargeable Zinc-Air Batteries.

As bifunctional oxygen evolution/reduction electrocatalysts, transition-metal-based single-atom-doped nitrogen-carbon (NC) matrices are promising successors of the corresponding noble-metal-based catalysts, offering the advantages of ultrahigh atom utilization efficiency and surface active energy. However, the fabrication of such matrices (e.g., well-dispersed single-atom-doped M-N4/NCs) often requires numerous steps and tedious processes. Herein, ultrasonic plasma engineering allows direct carbonization in a precursor solution containing metal phthalocyanine and aniline. When combining with the dispersion effect of ultrasonic waves, we successfully fabricated uniform single-atom M-N4 (M = Fe, Co) carbon catalysts with a production rate as high as 10 mg min-1. The Co-N4/NC presented a bifunctional potential drop of ΔE = 0.79 V, outperforming the benchmark Pt/C-Ru/C catalyst (ΔE = 0.88 V) at the same catalyst loading. Theoretical calculations revealed that Co-N4 was the major active site with superior O2 adsorption-desorption mechanisms. In a practical Zn-air battery test, the air electrode coated with Co-N4/NC exhibited a specific capacity (762.8 mAh g-1) and power density (101.62 mW cm-2), exceeding those of Pt/C-Ru/C (700.8 mAh g-1 and 89.16 mW cm-2, respectively) at the same catalyst loading. Moreover, for Co-N4/NC, the potential difference increased from 1.16 to 1.47 V after 100 charge-discharge cycles. The proposed innovative and scalable strategy was concluded to be well suited for the fabrication of single-atom-doped carbons as promising bifunctional oxygen evolution/reduction electrocatalysts for metal-air batteries.

A simple colorimetric detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification assay using hydroxynaphthol blue metal indicator.

A simple reverse transcription loop-mediated isothermal amplification combined with visual detection method (vRT-LAMP) assay was developed for rapid and specific detection of porcine epidemic diarrhea virus (PEDV) in this study, which overcomes the shortcomings of previously described RT-LAMP assays that require additional detection steps or pose a risk of cross-contamination. The assay results can be directly detected by the naked eye using hydroxynaphthol blue after incubating for 40 min at 62 °C. The assay specifically amplified PEDV RNA and no other viral nucleic acids. The limit of detection of the assay was less than 50 RNA copies per reaction, which was 100 times more sensitive than conventional reverse transcription polymerase chain reaction (RT-PCR) and comparable to real-time RT-PCR (RRT-PCR). In the clinical evaluation, the PEDV detection rate of vRT-LAMP was higher than that of RRT-PCR, showing 99 % concordance, with a kappa value (95 % confidence interval) of 0.97 (0.93-1.01). Considering the advantages of high sensitivity and specificity, simple and direct visual monitoring of the results, no possibility for cross-contamination, and being able to be used as low-cost equipment, the developed vRT-LAMP assay will be a valuable tool for detecting PEDV from clinical samples, even in resource-limited laboratories.