RESUMO
We studied the effect of biogenic ferrihydrite nanoparticles synthesized as a result of the culturing of Klebsiella oxytoca on Wistar rats with experimental toxic hemolytic anemia. The pathology was simulated by single intraperitoneal injection of phenylhydrazine hydrochloride. On day 4, the functional parameters of erythrocytes in rats corresponded to the state of toxic hemolytic anemia. It is shown that ferrihydrite nanoparticles suspension has chronic toxicity and causes morphological changes in organs (mainly in the spleen), which are characterized by accumulation of nanoparticles. Administration of phenylhydrazine induced systemic vascular damage and the formation of extramedullary hematopoietic foci, which indicated a compensatory activation in hematopoiesis in the liver and spleen. Injection of nanoparticles reduced discirculatory and necrotic changes in the kidneys.
Assuntos
Anemia Hemolítica , Nanopartículas , Ratos , Animais , Ratos Wistar , Anemia Hemolítica/induzido quimicamente , Anemia Hemolítica/patologia , Modelos TeóricosRESUMO
The objective of the present study was to develop the methods for the chemical and toxicological analysis of cyclopentolate contained in the material evidence for the purpose of forensic chemical and forensic toxicological expertises. The optimal conditions for the isolation of cyclopentolate from the cyclomed preparation and biological fluids were created using a chloroform-2-ptropanol mixture at pH of the medium 10.0. The substance of interest was identified with the use of the color and flocculation reactions, UV spectroscopy, thin layer chromatography and gas chromatography with the use of a mass-selective detector. HPLC and GCh/MS were employed for the quantitative determination. The results of the two methods are in excellent agreement.
Assuntos
Ciclopentolato/química , Toxicologia Forense/métodos , Detecção do Abuso de Substâncias/métodos , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia em Camada Fina/métodos , Ciclopentolato/toxicidade , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Parassimpatolíticos/química , Parassimpatolíticos/toxicidadeRESUMO
The objective of the present work was to study conditions for isolation of ketorolac and diclofenac from biological fluids. A method of their extraction with a mixture of organic solvents has been developed and the conditions for the identification of these compounds are proposed with the use of high performance liquid chromatography (HPLC), UV spectroscopy, and gas chromatography with electron capture detection (GC/ECD). The possibilities of using HPLC, UV spectrometry, and GC/ECD for quantitative determination of ketorolac and diclofenac are illustrated.
Assuntos
Diclofenaco/sangue , Cetorolaco/sangue , Cromatografia Gasosa/métodos , Cromatografia Líquida de Alta Pressão/métodos , Humanos , Sensibilidade e Especificidade , Espectrofotometria Ultravioleta/métodosRESUMO
Optimal conditions for the separation of diclofenac and ketorolac in the presence of other pharmaceutical products and narcotic substances are described with the help of gas and high performance liquid chromatography. The detection limits for individual compounds were established and the characteristic ions were identified. The possibility of application of IR- and UV-spectroscopy for the analysis of selected narcotic and non-narcotic analgesics was evaluated.
Assuntos
Anti-Inflamatórios/análise , Ciências Forenses/métodos , Anti-Inflamatórios/química , Cromatografia Gasosa/métodos , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas/métodos , Entorpecentes/análise , Entorpecentes/químicaRESUMO
This work was devoted to the elucidation of conditions for isolation of ketorolac and diclofenac from biological fluids. A method is proposed for the extraction of these compounds from solutions with organic solvents at different pH values. Other methods permit to optimize identification of analytes by thin layer chromatography while the densitometric technique may be used for qualitative and quantitative analysis of their composition in biological fluids.
Assuntos
Anti-Inflamatórios não Esteroides/análise , Líquidos Corporais/química , Cromatografia em Camada Fina/métodos , Diclofenaco/análise , Medicina Legal , Cetorolaco/análise , Animais , Anti-Inflamatórios não Esteroides/sangue , Anti-Inflamatórios não Esteroides/urina , Diclofenaco/sangue , Diclofenaco/urina , Humanos , Cetorolaco/sangue , Cetorolaco/urina , Camundongos , Ratos , Sensibilidade e EspecificidadeRESUMO
The conditions of butorphanol isolation from biological fluids were studied. The method of its extraction with the mix of organic solvents by pH 12 was proposed. How to identify butorphanol with the methods of thin-layer chromatography, ultraviolet spectrometry, high-performance liquid chromatography, gas chromatography with a detector of electron capture, chromato-mass spectrometry was developed. Possibility of use ultraviolet spectrometry for quantitative assessment of butorphanol was shown.
Assuntos
Analgésicos Opioides , Butorfanol , Toxicologia Forense/métodos , Preparações Farmacêuticas , Detecção do Abuso de Substâncias/métodos , Analgésicos Opioides/análise , Analgésicos Opioides/sangue , Analgésicos Opioides/urina , Animais , Butorfanol/análise , Butorfanol/sangue , Butorfanol/urina , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Preparações Farmacêuticas/química , Preparações Farmacêuticas/normas , RatosRESUMO
The conditions of doxilamine isolation from biological fluids are studied. The method of its extraction with a mixture of organic solvents in pH 9 is proposed. Identification of doxilamine with techniques of thin layer chromatography, UV-, IR-spectroscopy, chromato-mass-spectrometry, densitometry, gas-liquid chromatography is described. UV-spectrometry, gas chromatography and densitometry can be used for quantitation of doxilamine.