RESUMO
The quantitative ratios of RNA:DNA were followed in a developing transplanted fibrosarcoma in C3H mice and in its lung metastases. There was a significant increase in these ratios in developing tumors originating from cell suspensions (P smaller than 0.001) and a single implanted piece (P smaller than 0.05). No significant change was demonstrated in developing fibrosarcoma originating from two pieces of this tumor (P greater than 0.05) which were implanted simultaneously. When comparing the ratios of RNA and DNA of developing lung metastases to the primary tumors, we found a significantly higher ratio in the metastases (P smaller than 0.001). No significant changes in RNA:DNA ratios were demonstrated in normal proliferating tissues either in physiologic hyperplasia or embryo tissue culture (P greater than 0.05).
Assuntos
DNA de Neoplasias/metabolismo , Fibrossarcoma/metabolismo , RNA Neoplásico/metabolismo , Animais , Técnicas de Cultura , Embrião de Mamíferos/metabolismo , Feminino , Neoplasias Pulmonares/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C3H , Metástase Neoplásica , Transplante de Neoplasias , Gravidez , Sarcoma Experimental/metabolismo , Transplante Homólogo , Útero/metabolismoRESUMO
A study has been made of the quantitative relation existing between the RNA:DNA ratios in benign and in malignant human tumors. It has been demonstrated that a significant difference (Student's t-test) existed between malignant and benign human tumors within the different groups studied: a) malignant and benign breast tumors (t=2.22, P less than 0.05); b) Hodgkin's disease in spleen and its controls (t=2.39, P less than 0.02); and c) malignant and benign thyroid tumors (t=4.23, P less than 0.01). The diagnostic value of this finding is discussed.