Interobserver variability in the determination of bone mineral density in Hounsfield units from differently configured fields of measurement in the cancellous bone of vertebral bodies from elderly body donors.

Background: Due to the absence of suitable diagnostic procedures, osteoporosis (OP) is frequently detected late or not at all. Many elderly persons undergo computed tomographies (CT). The routine determination of Hounsfield units (HU) in bone as a part of these examinations could close a gap here. Methods: Spines were extracted from 22 body donors, fixed in a PVC water phantom, and subjected to a high-resolution CT investigation. Cancellous bone was examined and its bone mineral density measured in HU from cervical vertebra 3 to lumbar vertebra 5 (484 vertebral bodies). On sagittal sections, a circular and a rectangular region of interest (ROI) were defined in mid-vertebral cancellous bone, positioned manually, and the measurements were performed by three experienced radiologists. Bone mineral density (BMD), measured in mg/cm3, was used to determine the presence of OP. Results: All of the spines were osteoporotic. In the presence of a BMD below 60 mg/cm3 and HU values below 63.36 in lumbar vertebrae, there were significantly more vertebral body fractures in the thoracic and thoracolumbar spine. No difference was observed between the manually positioned circular and rectangular regions of interest (ROI) on the sagittal CT section (p > 0.05). Similar HU counts were obtained by the individual examiners (p > 0.05). The following formula was used to determine QCT values on a non-contrasted CT of the spine: QCT = 0.6 × HU + 13.7. Conclusions: Measurement of the density of cancellous bone in HU can be used to determine BMD for estimating demineralization. Quantitative BMD values in mg/cm3, which can be calculated from the HU data, concur well with QCT values.

[Insufficiency fractures of the spine in relation to cancellous bone density : An in vitro study]. / Insuffizienzfrakturen der Wirbelsäule in Abhängigkeit von der spongiösen Knochendichte : Eine in-vitro-Studie.

BACKGROUND: The risk of osteoporotic insufficiency fractures (Fx) at the axial skeleton increases with decreasing bone density, with an accumulation in the thoracic and thoracolumbar regions. To better understand the differential distribution of Fx along the spine, morphological and osteodensitometric studies were performed by computed tomography (CT) in the various spine sections. In addition, we aimed to clarify whether Hounsfield units (HU) found on CT examinations from other indications correlate with bone density and could be grounds for osteologic diagnosis. MATERIAL AND METHODS: The entire spines of 26 body donors were fixed in a Plexiglas water phantom and analyzed by high-resolution spiral CT. In addition, CT morphological cancellous bone density was measured in HU from C3 to S2 (624 vertebral bodies). Bone mineral density (BMD, mg/ml) was calculated and used to estimate osteoporosis (OPO). RESULTS: OPO was present in all spines. Significantly increased sintering fractures were found in the thoracic and thoracolumbar regions when BMD was below 60 mg/ml. Fx in the cervical spine area were not found overall. Cancellous bone density was significantly higher in the cervical (median 188.6 HU) than in the lumbar (median 63.6 HU) and sacral (median 25.5 HU) spine. DISCUSSION: BMD loss of vertebral body cancellous bone leads to an increased risk of Fx, which is also found in the cadaver spines. However, an apparent threshold for the occurrence of sintering fractures is not undercut in the cervical region. Finding a threshold for HU would be relevant to clinical practice.

Breaking strength and bone microarchitecture in osteoporosis: a biomechanical approximation based on load tests in 104 human vertebrae from the cervical, thoracic, and lumbar spines of 13 body donors.

BACKGROUND: The purpose of the study was to investigate associations between biomechanical resilience (failure load, failure strength) and the microarchitecture of cancellous bone in the vertebrae of human cadavers with low bone density with or without vertebral fractures (VFx). METHODS: Spines were removed from 13 body donors (approval no. A 2017-0072) and analyzed in regard to bone mineral density (BMD), Hounsfield units (HU), and fracture count (Fx) with the aid of high-resolution CT images. This was followed by the puncture of cancellous bone in the vertebral bodies of C2 to L5 using a Jamshidi™ needle. The following parameters were determined on the micro-CT images: bone volume fraction (BVF), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), degree of anisotropy (DA), trabecular number (Tb.N), trabecular pattern factor (Tb.Pf), and connectivity density (Conn.D). The axial load behavior of 104 vertebral specimens (C5, C6, T7, T8, T9, T12, L1, L3) was investigated with a servohydraulic testing machine. RESULTS: Individuals with more than 2 fractures had a significantly lower trabecular pattern factor (Tb.Pf), which also proved to be an important factor for a reduced failure load in the regression analysis with differences between the parts of the spine. The failure load (FL) and endplate sizes of normal vertebrae increased with progression in the craniocaudal direction, while the HU was reduced. Failure strength (FS) was significantly greater in the cervical spine than in the thoracic or lumbar spine (p < 0.001), independent of sex. BVF, Tb.Th, Tb.N, and Conn.D were significantly higher in the cervical spine than in the other spinal segments. In contrast, Tb.Sp and Tb.Pf were lowest in the cervical spine. BVF was correlated with FL (r = 0.600, p = 0.030) and FS (r = 0.763, p = 0.002). Microarchitectural changes were also detectable in the cervical spine at lower densities. CONCLUSIONS: Due to the unique microarchitecture of the cervical vertebrae, fractures occur much later in this region than they do in the thoracic or lumbar spine. Trial registration Approval no. A 2017-0072.

Why Insufficiency Fractures are Rarely Found in the Cervical Spine, Even with Osteoporosis. / Warum sich in der Halswirbelsäule auch bei Osteoporose nur selten Insuffizienzfrakturen finden.

INTRODUCTION: The human bone structure changes with an increase in age. Both material and structural properties affect bone strength. Despite the ageing of society, however, hardly any data are available on these parameters for elderly individuals. Therefore, in the present study, cancellous bone cylinders were taken from the center of each vertebral body (C3 to L5) and examined with regard to bone volume fraction, trabecular thickness, separation, number of trabeculae, cross-linking, connectivity density and degree of anisotropy. MATERIAL AND METHODS: Samples were obtained from 440 body donors using a Jamshidi needle and analysed using microcomputed tomography. Existing deformities, fractures and bone mineral density of each vertebra were recorded by quantitative computed tomography. RESULTS: With regard to the microcomputed tomography parameters, statistically significant differences were found between the different sections of the vertebrae: the trabeculae of the cervical vertebrae were significantly thicker and more closely spaced than in the thoracic and lumbar vertebrae. The bone volume fraction was significantly higher in this spinal segment, as was the connection density and the number of trabeculae and cross-links. In addition, the degree of anisotropy was significantly lower in the cervical vertebrae than in the other spinal segments. With regard to quantitative computed tomography, there was a significantly higher bone mineral density in the cervical vertebrae. CONCLUSION: Even with osteoporosis, cervical vertebrae fracture significantly later than thoracic and lumbar vertebrae due to their unique microarchitecture and higher density. Thus, the cervical vertebrae has specific properties.

A comparison, using X-ray micro-computed tomography, of the architecture of cancellous bone from the cervical, thoracic and lumbar spine using 240 vertebral bodies from 10 body donors.

The vertebral trabecular bone has a complex three-dimensional microstructure with an inhomogeneous morphology. Correct identification and assessment of the weakest segments of the cancellous bone may lead to better prediction of fracture risk. The aim of this study was to compare cancellous bone from 240 vertebrae of the cervical, thoracic and lumbar spine of ten body donors with osteoporosis in regard to bone volume fraction (BVF), trabecular thickness, separation, trabecular number and degree of anisotropy, to ascertain why cervical vertebrae rarely fracture, even with severe osteoporosis. Samples were obtained from all vertebrae with a Jamshidi needle (8 Gauge). The investigations were performed with a micro-computed tomography (micro-CT) device (SKYSCAN 1172, RJL Micro & Analytic GmbH, Karlsdorf-Neuthard, Germany). Existing vertebral fractures and the bone mineral density of the lumbar spine were assessed with quantitative CT. Regarding the micro-CT parameters, statistically significant differences were observed between the various sections of the spine. We found a higher BVF, trabecular number and trabecular thickness, as well as a lower trabecular separation of the cervical vertebrae compared to other vertebrae. In addition, the degree of anisotropy in the cervical spine is lower than in the other spinal column sections. These results are age and sex dependent. Thus, the cervical spine has special structural features, whose causes must be determined in further investigations.

How context alters value: The brain's valuation and affective regulation system link price cues to experienced taste pleasantness.

Informational cues such as the price of a wine can trigger expectations about its taste quality and thereby modulate the sensory experience on a reported and neural level. Yet it is unclear how the brain translates such expectations into sensory pleasantness. We used a whole-brain multilevel mediation approach with healthy participants who tasted identical wines cued with different prices while their brains were scanned using fMRI. We found that the brain's valuation system (BVS) in concert with the anterior prefrontal cortex played a key role in implementing the effect of price cues on taste pleasantness ratings. The sensitivity of the BVS to monetary rewards outside the taste domain moderated the strength of these effects. These findings provide novel evidence for the fundamental role that neural pathways linked to motivation and affective regulation play for the effect of informational cues on sensory experiences.