Potential use of lime combined with additives on (im)mobilization and phytoavailability of heavy metals from Pb/Zn smelter contaminated soils.

This explorative study was aimed to assess the efficiency of lime alone and in combined with additives to immobilize Pb, Cd, Cu and Zn in soil and reduce their phytoavailability for plant. A greenhouse pot experiment was performed by using low and heavily contaminated top soils viz. Tongguan contaminated (TG-C); Fengxian heavily contaminated (FX-HC) and Fengxian low contaminated (FX-LC). The contaminated soils were treated with lime (L) alone and in combined with Ca-bentonite (CB), Tobacco biochar (TB) and Zeolite (Z) at 1% and cultivated by Chinese cabbage (Brassica campestris L). Results revealed that all amendments (p< 0.05) significantly reduced the DTPA-extractable Pb 97.33, Cd 68.06 and Cu 91.11% with L+TB, L+CB, L+Z in FX-LC soil and Zn 87.12% respectively, with L+CB into TG-C soil. Consequently, the application of lime alone and in combined with additives were drastically decreased the dry biomass yield of Brassica campestris L. as compared with control. Thus, these feasible amendments potentially maximum reduced the uptake by plant shoots upto Pb 53.47 and Zn 67.93% with L+Z and L+TB in FX-LC soil, while Cd 68.58 and Cu 60.29% with L+TB, L+CB in TG-C soil but Cu uptake in plant shoot was observed 27.26% and 30.17% amended with L+TB and L+Z in FX-HC and FX-LC soils. On the other hand, these amendments were effectively reduced the potentially toxic metals (PTMs) in roots upto Pb77.77% L alone in FX-HC, Cd 96.76% with L+TB in TG-C, while, Cu 66.70 and Zn 60.18% with L+Z in FX-LC. Meanwhile, all amendments were responsible for increasing soil pH and CEC but decreased soils EC level. Based on this result, these feasible soil amendments were recommended for long term-study under field condition to see the response of another hyper accumulator crop.

Futuristic advancements in phytoremediation of endocrine disruptor Bisphenol A: A step towards sustainable pollutant degradation for rehabilitated environment.

Bisphenol A (BPA) accumulates in the environment at lethal concentrations because of its high production rate and utilization. BPA, originating from industrial effluent, plastic production, and consumer products, poses serious risks to both the environment and human health. The widespread aggregation of BPA leads to endocrine disruption, reactive oxygen species-mediated DNA damage, epigenetic modifications and carcinogenicity, which can disturb the normal homeostasis of the body. The living being in a population is subjected to BPA exposure via air, water and food. Globally, urinary analysis reports have shown higher BPA concentrations in all age groups, with children being particularly susceptible due to its occurrence in items such as milk bottles. The conventional methods are costly with a low removal rate. Since there is no proper eco-friendly and cost-effective degradation of BPA reported so far. The phytoremediation, green-biotechnology based method which is a cost-effective and renewable resource can be used to sequestrate BPA. Phytoremediation is observed in numerous plant species with different mechanisms to remove harmful contaminants. Plants normally undergo several improvements in genetic and molecular levels to withstand stress and lower levels of toxicants. But such natural adaptation requires more time and also higher concentration of contaminants may disrupt the normal growth, survival and yield of the plants. Therefore, natural or synthetic amendments and genetic modifications can improve the xenobiotics removal rate by the plants. Also, constructed wetlands technique utilizes the plant's phytoremediation mechanisms to remove industrial effluents and medical residues. In this review, we have discussed the limitations and futuristic advancement strategies for degrading BPA using phytoremediation-associated mechanisms.

Comprehensive review on recent production trends and applications of biochar for greener environment.

The suitability of biochar as a supplement for environmental restoration varies significantly based on the type of feedstocks used and the parameters of the pyrolysis process. This study comprehensively examines several aspects of biochar's potential benefits, its capacity to enhance crop yields, improve nutrient availability, support the co-composting, water restoration and enhance overall usage efficiency. The supporting mechanistic evidence for these claims is also evaluated. Additionally, the analysis identifies various gaps in research and proposes potential directions for further exploration to enhance the understanding of biochar application. As a mutually advantageous approach, the integration of biochar into agricultural contexts not only contributes to environmental restoration but also advances ecological sustainability. The in-depth review underscores the diverse suitability of biochar as a supplement for environmental restoration, contingent upon the specific feedstock sources and pyrolysis conditions used. However, concerns have been raised regarding potential impacts on human health within agricultural sectors.

Biochar preparation and evaluation of its effect in composting mechanism: A review.

This article provides an overview of biochar application for organic waste co-composting and its biochemical transformation mechanism. As a composting amendment, biochar work in the adsorption of nutrients, the retention of oxygen and water, and the promotion of electron transfer. These functions serve the micro-organisms (physical support of niche) and determine changes in community structure beyond the succession of composing primary microorganisms. Biochar mediates resistance genes, mobile gene elements, and biochemical metabolic activities of organic matter degrading. The participation of biochar enriched the α-diversity of microbial communities at all stages of composting, and ultimately reflects the high γ-diversity. Finally, easy and convincing biochar preparation methods and characteristic need to be explored, in turn, the mechanism of biochar on composting microbes at the microscopic level can be studied in depth.

Neem extract-blended nanocellulose derived from jackfruit peel for antibacterial packagings.

The use of jackfruit peel as a source for natural and fully biodegradable "nanocellulose" (NC) for the production of bioplastics with Azadirachta indica (A. indica) extracts and polyethylene glycol (PEG) for the antibacterial properties is investigated. The characterization of the biocomposite using FT-IR and WXRD was reported. The physicochemical properties including thickness, moisture content, water holding capacity, swelling, porosity, and biodegradability in soil were investigated. The incorporation of A. indica extract revealed an increased shelf life due to the strong antibacterial activity, and these biocomposites were degraded in soil within 60 days after the end use without any harm to the environment. Jackfruit-derived nanocellulose film blended with A. indica extract exhibited strong antibacterial activity against gram-positive and gram-negative food spoilage bacteria. Disc diffusion assay, live/dead assay, and CFU analysis confirmed the antibacterial property of the synthesized film. Moreover, the films clearly prevented the biofilm formation in bacteria. Thus, the developed bioplastics can be utilized as appropriate substitutes to food packaging materials and also for biomedical applications such as wound dressings.

Effects of magnesite on nitrogen conversion and bacterial community during pig manure composting.

The objective of this research was to elucidate the effect of varying proportions of magnesite (MS) addition - 0% (T1), 2.5% (T2), 5% (T3), 7.5% (T4), and 10% (T5) - on nitrogen transformation and bacterial community dynamics during pig manure composting. In comparison to T1 (control), MS treatments amplified the abundance of Firmicutes, Actinobacteriota, and Halanaerobiaeota, bolstered the metabolic functionality of associated microorganisms, and enhanced the nitrogenous substance metabolic pathway. A complementary effect in core bacillus species played a key role in nitrogen preservation. Compared to T1, 10% MS demonstrated the most substantial influence on composting because Total Kjeldahl Nitrogen increased by 58.31% and NH3 emission decreased by 41.52%. In conclusion, 10% MS appears to be optimal for pig manure composting, as it can augment microbial abundance and mitigate nitrogen loss. This study offers a more ecologically sound and economically viable method for curtailing nitrogen loss during composting.

Regulation of fungal communities during pig manure composting.

The role of protein shell (PS) amendment in altering the fungal community during pig manure (PM) composting was investigated. Six different dosages of PS based on the dry weight of PM (0 %, 2.5 %, 5 %, 7.5 %, 10 %, and 12 %; T1-T6, respectively) were mixed with wheat straw to make the initial feedstock and composted for 42 days. The results showed that Ascomycota, Basidiomycota, and Giomeromycota were the most abundant phyla in all treatments. However, the relative abundance of Giomeromycota was the highest in the control treatment, although a substantially greater population was observed in all treatments. Genus abundance declined steadily from T1 to T6; however, T4 and T6 had smaller populations. Correlation analysis also suggested that T6 amendment increased the overall fungal dynamics and organic matter degradation. Thus, T6 was more efficient to enhance the overall fungal population and dynamics with considerable network connections among all the analyzed parameters.

Biochar as smart organic catalyst to regulate bacterial dynamics during food waste composting.

The impact of wheat straw biochar (WSB) on bacterial dynamics succession during food waste (FW) composting was analyzed. Six treatments [0(T1), 2.5(T2), 5 (T3), 7.5 (T4), 10 (T5), and 15 %(T6)] dry weight WSB were used with FW and saw dust for composting. At the highest thermal peak at 59 ℃ in T6, the pH varied from 4.5 to 7.3, and electrical conductivity among the treatments varied from 1.2 to 2.0 mScm1. Firmicutes (25-97 %), Proteobacteria (8-45 %), and Bacteroidota (5-50 %) were among the dominate phyla of the treatments. Whereas, Bacillus (5-85 %), Limoslactobacillus (2-40 %), and Sphingobacterium (2-32 %) were highest among the identified genus in treatments but surprisingly Bacteroides was in greater abundance in the control treatments. Moreover, heatmap constructed with 35 various genera in all the treatments showed that Gammaproteobacterial genera contributed in large proportion after 42 days in T6. Additionally, a dynamic shift from Lactobacillus fermentum to higher abundance of Bacillus thermoamylovorans was reported on 42 days of FW composting. Biochar 15 % amendment can improve FW composting by influencing bacterial dynamics.

Advanced biofuel production, policy and technological implementation of nano-additives for sustainable environmental management - A critical review.

This review article critically evaluates the significance of adopting advanced biofuel production techniques that employ lignocellulosic materials, waste biomass, and cutting-edge technology, to achieve sustainable environmental stewardship. Through the analysis of conducted research and development initiatives, the study highlights the potential of these techniques in addressing the challenges of feedstock supply and environmental impact and implementation policies that have historically plagued the conventional biofuel industry. The integration of state-of-the-art technologies, such as nanotechnology, pre-treatments and enzymatic processes, has shown considerable promise in enhancing the productivity, quality, and environmental performance of biofuel production. These developments have improved conversion methods, feedstock efficiency, and reduced environmental impacts. They aid in creating a greener and sustainable future by encouraging the adoption of sustainable feedstocks, mitigating greenhouse gas emissions, and accelerating the shift to cleaner energy sources. To realize the full potential of these techniques, continued collaboration between academia, industry representatives, and policymakers remains essential.

Recent advances in lignocellulosic and algal biomass pretreatment and its biorefinery approaches for biochemicals and bioenergy conversion.

As the global demand for sustainable energy increases, lignocellulosic (such as agricultural residues, forest biomass, municipal waste, and dedicated energy crops) and algal (including macroalgae and microalgae) biomass have attracted considerable attention, because of their high availability of carbohydrates. This is a potential feedstock to produce biochemical and bioenergy. Pretreatment of biomass can disrupt their complex structure, increasing conversion efficiency and product yield. Therefore, this review comprehensively discusses recent advances in different pretreatments (physical, chemical, physicochemical, and biological pretreatments) for lignocellulosic and algal biomass and their biorefining methods. Life cycle assessment (LCA) which enables the quantification of the environmental impact assessment of a biorefinery also be introduced. Biorefinery processes such as raw material acquisition, extraction, production, waste accumulation, and waste conversion are all monitored under this concept. Nevertheless, there still exist some techno-economic barriers during biorefinery and extensive research is still needed to develop cost-effective processes.

Effect of scleral protein shell amendment on bacterial community succession during the pig manure composting.

The impact of scleral protein shell (SPS) amendment on bacterial community succession during pig manure (PM) composting were evaluated in the present work. Five treatments representing different dry weight dosage of SPS [0 % (T1), 2.5 % (T2), 5 % (T3), 7.5 % (T4), 10 % (T5) and 12 % (T6)] were applied with initial mixture of raw materials (Wheat straw along with the PM) and composted for 42 days. Results indicated that the dominant of phyla were Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes. The relative abundance (RA) of genus un-identified, Ruminofilibacter, Thermovum, Longispora and Pseudomonas were greater among the all treatments but interestingly genus Ruminofilibacter was also higher in control treatment. The network analysis was confirmed that T6 treatment with higher dosage of SPS amendment could enhance the bacterial population and rate of organic matter mineralization. Compared with T1, the T5 has greater potential impact to enhance the bacterial population and significant correlation among the pH and temperature.

Genetic manipulation strategies for ethanol production from bioconversion of lignocellulose waste.

Lignocellulose waste was served as promising raw material for bioethanol production. Bioethanol was considered to be a potential alternative energy to take the place of fossil fuels. Lignocellulosic biomass synthesized by plants is regenerative, sufficient and cheap source for bioethanol production. The biotransformation of lignocellulose could exhibit dual significance-reduction of pollution and obtaining of energy. Some strategies are being developing and increasing the utilization of lignocellulose waste to produce ethanol. New technology of bioethanol production from natural lignocellulosic biomass is required. In this paper, the progress in genetic manipulation strategies including gene editing and synthetic genomics for the transformation from lignocellulose to ethanol was reviewed. At last, the application prospect of bioethanol was introduced.

Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC).

This study uses a cost effective and efficient method for production of higher DP (degree of polymerization) Xylooligosaccharides (XOS) from xylan extracted from the waste walnut shells. Copper based metal organic framework (Cu-BTC MOF) was prepared for immobilization of free xylanase (Xy) enzyme by green synthesis method. Both free and immobilized xylanase (Xy-Cu-BTC) were able to cause the bioconversion of xylan (87.4% yield) into XOS. Predominant production of xylotetrose (X4) and xylopentose (X5) was observed for both the methods. Percentage XOS conversion for free enzyme (Xy) was found to be 4.1% X4 and 60.57% X5 whereas these values increased in case of immobilized system where 11.8% X4 and 64.2% X5 were produced. Xylose production was minute in case of immobilized xylanase 0.88% which makes it a better method for XOS production free from xylose interference. Xy-Cu-BTC MOF can hence be used as an attractive alternative for pure XOS production.

Nanocellulose in tissue engineering and bioremediation: mechanism of action.

Nanocellulose are nano-sized components which are biodegradable, biocompatible and renewable. It offers mechanical strength and chemical stability in plants and bacteria. The environmental contamination is reduced by employing various bioremediation techniques which usesmicroorganisms like algae, bacteria and fungi as bio-adsorbents. The bio adsorbent property of nanocellulose contribute more for the bioremediation methods and the detailed study of its mechanism and application is essential which is discussed here. The mechanism happening between the contaminant and nanocellulose adsorbent should be explored in detail in order to develop effective new bioremediation strategies. Nanocellulose structural functionalization helps to modify the nanocellulose structure based on which it can be utilized for specific functions. Exploring the mechanisms that contribute to the implementation of nanocellulose in tissue engineering helps for further developments and advancement in the biomedical application of nanocellulose. Not much studies are available that elucidate and study the basic steps involved in the biomedical and environmental usage of nanocellulose. This review has focussed on the basic mechanisms involved in the use of nanocellulose in tissue engineering and bioremediation processes.

Magnesite driven the complementary effects of core fungi by optimizing the physicochemical parameters in pig manure composting.

The effects of magnesite (MS) on fungi communities and the core fungi complementarity during pig manure (PM) composting were explored. Different dosage of MS [0% (T1), 2.5% (T2), 5% (T3), 7.5% (T4) and 10% (T5)] as amendments mixed with PM for 42 days composting. The results showed the dominant of phyla were Ascomycota (78.87%), Neocallimastigomycota (41.40%), Basidiomycota (30.81%) and Aphelidiomycota (29.44%). From day 7 to 42, the abundance of Ascomycota and Aphelidiomycota were increased from 7.75% to 42.41% to 57.27%-78.87% and 0-0.70% to 11.73%-29.44% among all treatments. Nevertheless, the phyla abundance of Neocallimastigomycota and Basidiomycota decreased from day 7 to 42. The co-occurrence network indicated that the high additive amendment could enhance the core fungi complementarity effects capacity. The 10% MS addition was a promisable candidate to optimum fungal communities, and causing a better compost quality. This study illustrated the potential and fungi communities changing of MS as additives in composting.

Enhanced removal of humic acid from piggery digestate by combined microalgae and electric field.

Microalgae technology is a promising method for treating piggery digestate, while its removal ability of humic acids (HAs) is poor. Here, an electric field-microalgae system (EFMS) was used to improve the removal of HAs from the piggery digestate. Results indicated that the removal of HAs by EFMS relied on the initial concentration of HAs, electrical intensity, the initial inoculation concentration of microalgae and pH. Values of these parameters were optimized as electrical intensity of 1.2 V/cm, microalgae initial inoculation concentration of 0.1 g/L and pH 5.0. The HAs removal efficiency by EFMS (55.38%) was 13% and 38% higher than that by single electric field and microalgal technology. It was observed that oxidation, coagulation and assimilation contributed to the removal of HAs, suggesting that EFMS could serve as an attractive and cost-effective technique for the removal of HAs from the piggery digestate.

Multifunctional applications of bamboo crop beyond environmental management: an Indian prospective.

Increasing population, industrialization, and economic growth cause several adverse impacts on the existing environment and living being. Therefore, rising pollutants load and their mitigation strategies, as well as achieving energy requirements while reducing reliance on fossil fuels are the key areas, which needs significant consideration for sustainable environment. Since India has considerable biomass resources, bioenergy is a significant part of the country's energy policy. However, the selection of feedstock is a crucial step in bioenergy production that could produce raw material without compromising food reserve along with the sustainable environment. Higher growth capacity of bamboo species makes them a suitable lignocellulosic substrate for the production of high-value greener products such as fuels, chemicals, and biomaterials as well as an appropriate candidate for eco-restoration of degraded land. In that context, the current review discusses the multidimensional applications of bamboo species in India. The bioenergy potency of bamboo and probability of aligning its production, cultivation, and operation with economic and social development agendas are also addressed, making it an exceptional crop in India. Additionally, its fast growth, perennial root systems, and capability to restore degraded land make it an essential part of ecological restoration. Furthermore, this review explores additional benefits of bamboo plantation on the environment, economy, and society along with future research prospects.

Activities of functional enzymes involved in C, N, and P conversion and their stoichiometry during agricultural waste composting with biochar and biogas residue amendments.

This experiment was carried out to explore the effects of biochar, biogas residue and their combination amendment on enzyme activities and their stoichiometry during agricultural waste composting. A comprehensive analysis of the variation in, and stoichiometric correlations between, ß-glucosidase (BG), N-acetylglucosaminidase (NAG), leucine aminopeptidase (LAP), and alkaline phosphatase (AKP) were determined. The results showed that biochar, biogas residue, and their combined addition significantly increased those enzyme activities. The potential C:P and N:P acquisition activities represented by ln(BG): ln(AKP) and ln(LAP + NAG): ln(AKP), were significantly decreased with biogas residue addition. BG, NAG and LAP were significantly negatively correlated with temperature, organic matter and water-soluble carbon. Redundancy analysis also showed that moisture and water-soluble carbon were significantly related to the variations of enzyme activities. Biochar and biogas residue changed the characteristics of the composting substrate, thus affecting the activity and stoichiometry of functional enzymes involved in C, N and P cycling.

Exergy analysis and optimization of bio-methane production from corn stalk pretreated by compound bacteria based on genetic algorithm.

An exergy equilibrium model was established to obtain the exergy efficiency under different conditions of compound bacteria pretreatment and anaerobic digestion (AD) of corn stalk. The Genetic Algorithm (GA) was applied to optimize the exergy efficiency of the combination process of the pretreatment and AD. The maximum exergy efficiency with the GA was 19.04%, corresponding to the optimal pretreatment parameters: pretreatment temperature 33.34℃, stalk particle size 0.50 mm, ventilation rate 0.88 L/min, pretreatment time 169.03 h. The optimal AD parameters were: digestion temperature 38.08℃ and stirring rate 48.04 r/min. The validation experiment exergy efficiency reached to 19.25%, which was 24.37% higher as compared to that of the non-pretreatment process. Under these optimal conditions, the energy consumption of the compound bacteria pretreatment and the time of the bio-methane production process were effectively reduced.

Synthesis of C2-C4 diols from bioresources: Pathways and metabolic intervention strategies.

Diols are important platform chemicals with extensive industrial applications in biopolymer synthesis, cosmetics, and fuels. The increased dependence on non-renewable sources to meet the energy requirement of the population raised issues regarding fossil fuel depletion and environmental impacts. The utilization of biological methods for the synthesis of diols by utilizing renewable resources such as glycerol and agro-residual wastes gained attention worldwide because of its advantages. Among these, biotransformation of 1,3-propanediol (1,3-PDO) and 2,3-butanediol (2,3-BDO) were extensively studied and at present, these diols are produced commercially in large scale with high yield. Many important isomers of C2-C4 diols lack natural synthetic pathways and development of chassis strains for the synthesis can be accomplished by adopting synthetic biology approaches. This current review depicts an overall idea about the pathways involved in C2-C4 diol production, metabolic intervention strategies and technologies in recent years.