RESUMO
Increased activities of certain biochemical enzymes (alanine aminotransferase [ALT], aspartate aminotransferase [AST], lactate dehydrogenase [LDH], alkaline phosphatase [ALP]) have been associated with blunt liver injury in many species. To evaluate changes in plasma hepatic biochemical parameters in acute avian liver disease caused by trauma and to compare biochemical changes with histologic lesions in hepatic parenchyma, 30 healthy fasted Indian ring-necked parakeets (Psittacula krameri manillensis) were divided into 2 groups, and traumatic liver injury was caused by endoscopic liver biopsy (group 1) or by liver biopsy and crushing injury to the hepatic parenchyma with endoscopic forceps (group 2) in anesthetized birds. Blood samples were collected at baseline and at 12, 24, 36, 48, 60, 72, 84, 96, 108, and 120 hours in alternate groups to compare analyte values after injury with those at baseline. Results showed consistently decreased plasma ALP activity (excluding 1 time point) throughout the study, which was thought to be associated with isoflurane administration. Plasma glutamate dehydrogenase activity initially increased but rapidly declined thereafter and was attributed to acute focal hepatocellular injury. In both groups, increases in plasma AST, ALT, and LDH activities was most likely caused by muscle injury because creatine kinase activity was concurrently increased. Compared with baseline values, bile acid concentration and y-glutamyl transferase activity were not affected by liver biopsy or crush injury. Plasma sorbitol dehydrogenase activity was the most specific indicator of liver injury in both groups. Histologic changes correlated poorly with biochemical results, possibly because the small area of hepatic parenchyma that was damaged did not affect enzyme values substantially.
Assuntos
Doenças das Aves/patologia , Fígado/lesões , Psittacula/lesões , Ferimentos e Lesões/veterinária , Animais , Feminino , Masculino , Ferimentos e Lesões/patologiaRESUMO
The National Toxicology Program (NTP) Satellite Symposium is a one-day meeting that is held in conjunction with the annual Society of Toxicologic Pathology (STP) meeting. The topic of the 2009 Symposium was "Tumor Pathology and INHAND (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) Nomenclature." The goal of this article is to provide summaries of each speaker's presentation, including the diagnostic or nomenclature issues that were presented, along with a few select images that were used for voting. The results of the voting process and interesting points of discussion that were raised during the presentation are also provided. A supplemental file with voting choices and voting results for each case presented at the symposium is available at http://tpx.sagepub.com/supplemental.
Assuntos
Neoplasias/patologia , Medula Suprarrenal/patologia , Animais , Proliferação de Células , Colangiocarcinoma/patologia , Imuno-Histoquímica , Neoplasias Hepáticas/patologia , Meningioma/patologia , Camundongos , Ratos , Terminologia como AssuntoRESUMO
Osteogenic melanoma is a rare variant of metaplastic malignant melanoma in human medicine and appears to be a similarly rare variant in dogs. Two dogs with oral malignant melanoma with neoplastic bone formation are reported in this study. Both tumors were characterized by malignant melanocytes that transitioned into neoplastic bone at the deep margins of the neoplasm. Immunohistochemical analysis revealed S100- and Melan-A-positive neoplastic cells adjacent to, and occasionally embedded within, an osteoid and chondroblastic matrix. Scattered clusters of neoplastic cells were also positive for osteocalcin. The findings indicate that in dogs, as in humans, neoplastic melanocytes have metaplastic potential and can be osteogenic.
Assuntos
Doenças do Cão/patologia , Neoplasias Gengivais/veterinária , Melanoma/veterinária , Ossificação Heterotópica/veterinária , Animais , Cães , Feminino , Neoplasias Gengivais/patologia , Melanoma/patologia , Ossificação Heterotópica/patologiaRESUMO
Although cytauxzoonosis has historically been nearly 100% fatal in domestic cats, increasing number of reports of infected cats that demonstrate less-severe disease suggest the existence of different strains of Cytauxzoon felis. To test this hypothesis, the genetic variability of C. felis was examined in blood samples from naturally infected domestic cats from Arkansas and Georgia by using the first and second ribosomal internal transcribed spacer regions (ITS1, ITS2) as markers to assess genotypic variability. In addition, the clinical outcome of infection (survival vs. fatal disease) was analyzed. Within the C. felis ITS1 region, there were a total of 8 single nucleotide polymorphisms (SNP) and a single nucleotide insertion. Within the ITS2 region, there were a total of 4 SNPs and a single 40 base pair insertion. When taken together, the ITS1 and ITS2 sequence data defined a total of 11 different sequences and 3 unique genotypes. One unique ITS1-ITS2 genotype was detected in samples submitted exclusively from Arkansas, and a second unique genotype was submitted exclusively from Georgia. There was a significant association between infection with C. felis that contained particular ITS genotypes and survival of the infected domestic cat. The identification of unique C. felis genotypes obtained from different geographic areas and the association of particular ITS genotypes with the outcome of infection suggest the existence of parasite strains that may vary in pathogenicity to the domestic cat and offer an explanation for the survival of some infected cats in more recent case studies.
Assuntos
Doenças do Gato/parasitologia , Eucariotos/genética , Infecções Protozoárias em Animais/parasitologia , Animais , Arkansas/epidemiologia , Doenças do Gato/epidemiologia , Gatos , DNA de Protozoário/genética , Eucariotos/patogenicidade , Variação Genética , Georgia/epidemiologia , Reação em Cadeia da Polimerase/veterinária , Infecções Protozoárias em Animais/epidemiologia , VirulênciaRESUMO
Cytauxzoon felis infection in domestic cats has historically been nearly 100% fatal. However, increasing reports of domestic cats that survive cytauxzoonosis and reports of asymptomatic cats with C. felis infections suggest the existence of different parasite strains that vary in pathogenicity. The objective of the current study was to obtain epidemiologic information about cytauxzoonosis through genotypic characterization of archived histologic specimens from domestic cats with C. felis infections that were diagnosed in Georgia between 1995 and 2007. Such retrospective data on genetic variability will provide an historic context for current studies of C. felis genotype frequencies. Cytauxzoon felis DNA was obtained from formalin-fixed, paraffin-embedded tissues from infected cats diagnosed with cytauxzoonosis at necropsy. Genetic characterization of C. felis was performed using sequence analysis of the polymerase chain reaction-amplified ribosomal internal transcribed spacer regions 1 and 2 (ITS1, ITS2). Eleven different combined ITS1 and ITS2 sequences were identified, the majority of which were identical to those previously reported in fatally infected cats from Georgia. The findings of the current study document the existence of genetically distinct C. felis populations in historical samples and, together with data from contemporary samples, demonstrate a diverse population structure for C. felis.
Assuntos
Doenças do Gato/parasitologia , Variação Genética , Piroplasmida/genética , Infecções Protozoárias em Animais/parasitologia , Animais , Doenças do Gato/epidemiologia , Gatos , DNA Intergênico/genética , DNA de Protozoário/genética , Georgia/epidemiologia , Infecções Protozoárias em Animais/epidemiologia , Estudos RetrospectivosRESUMO
A novel siadenovirus was identified in the Sulawesi tortoise (Indotestudo forsteni). A group of 105 Sulawesi tortoises was obtained by the Turtle Survival Alliance. Many of the tortoises were in poor health. Clinical signs included anorexia, lethargy, mucosal ulcerations and palatine erosions of the oral cavity, nasal and ocular discharge, and diarrhea. Initial diagnostic tests included fecal testing for parasites, complete blood count and plasma biochemical analysis, mycoplasma serology, and polymerase chain reaction (PCR) testing for intranuclear coccidia and chelonian herpesvirus. Treatment included administration of antibiotics, antiparasitic medications, parenteral fluids, and nutritional support. Tissue samples from animals that died were submitted for histopathologic evaluation. Histopathologic examination revealed systemic inflammation and necrosis associated with intranuclear inclusions consistent with a systemic viral infection in 35 tortoises out of 50 examined. Fecal testing results and histopathologic findings revealed intestinal and hepatic amoebiasis and nematodiasis in 31 animals. Two of 5 tortoises tested by PCR were positive for Chlamydophila sp. Aeromonas hydrophila and Escherichia coli were cultured from multiple organs of 2 animals. The mycoplasma serology and PCR results for intranuclear coccidia and chelonian herpesvirus were negative. Polymerase chain reaction testing of tissues, plasma, and choanal/cloacal samples from 41 out of 42 tortoises tested were positive for an adenovirus, which was characterized by sequence analysis and molecular phylogenetic inference as a novel adenovirus of the genus Siadenovirus. The present report details the clinical and anatomic pathologic findings associated with systemic infection of Sulawesi tortoises by this novel Siadenovirus, which extends the known reptilian adenoviruses to the chelonians and extends the known genera of reptilian Adenoviridae beyond Atadenovirus to include the genus Siadenovirus.
Assuntos
Infecções por Adenoviridae/veterinária , Siadenovirus/genética , Siadenovirus/isolamento & purificação , Tartarugas , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/patologia , Infecções por Adenoviridae/virologia , Sequência de Aminoácidos , Animais , Osso e Ossos/ultraestrutura , Osso e Ossos/virologia , DNA Polimerase Dirigida por DNA/química , DNA Polimerase Dirigida por DNA/genética , DNA Polimerase Dirigida por DNA/metabolismo , Indonésia/epidemiologia , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase/veterinária , Baço/ultraestrutura , Baço/virologia , Proteínas não Estruturais Virais/química , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/metabolismoRESUMO
All fledgling canaries (Serinum canarius) in a small private aviary died from atoxoplasmosis during a single breeding season. The birds were clinically normal when removed from their parents at 2 mo of age, but by 3 mo of age all had died following an illness characterized by progressive lethargy, anorexia, and debilitation. Cachexia, splenomegaly, and pale foci in the liver were evident at necropsy. Microscopically, there was striking infiltration of the intestinal lamina propria by mononuclear cells that contained intracytoplasmic protozoa. Protozoa were also observed in mononuclear cells in splenic and hepatic sinusoids and in vascular or perivascular spaces of other organs, but were much less numerous. Ultrastructural features of infected enteric mononuclear cells were suggestive of lymphocytes, and the majority of parasitized cells in paraffin sections of intestine were positive for CD-79 antigen, consistent with B lymphocytes. CD-3 staining was minimal, suggesting little or no T-cell infection. The following year, after egg-laying was completed, adults were treated with sulfadimethoxine, and no further fledgling losses occurred.
Assuntos
Apicomplexa , Linfócitos B/parasitologia , Doenças das Aves/parasitologia , Canários , Enterite/veterinária , Infecções Protozoárias em Animais/parasitologia , Animais , Anti-Infecciosos/farmacologia , Doenças das Aves/tratamento farmacológico , Doenças das Aves/prevenção & controle , Enterite/tratamento farmacológico , Enterite/parasitologia , Enterite/prevenção & controle , Infecções Protozoárias em Animais/prevenção & controle , Sulfadimetoxina/farmacologiaRESUMO
Nineteen map turtles (Graptemys spp.) maintained under natural conditions were investigated because of chronic shell abnormalities. Animals were evaluated using a novel shell scoring system that divided the 54 scutes into six regions, with each region scored for lesion extent and severity, and summated to produce a total shell disease score (TSDS). Complete blood counts and various biochemistry analytes (total protein, albumin, globulin, urea, uric acid, 25-hydroxycholecalciferol, phosphorus, and ionized and total calcium) were measured. Under ketamine-medetomidine-morphine anesthesia, cytology tape strips and full thickness shell biopsies were collected aseptically for microbiologic, histologic (including scoring of biopsy quality), and ultrastructural evaluations. The TSDSs were low and ranged from 4 to 22 (median = 9) out of a possible score of 54. There were no correlations between TSDS and any hematologic or biochemistry parameter. The histologic quality of shell biopsies was good, and normal shell structure, by both light and electron microscopy, is described. Small clefts and pitting lesions were noted in 8/19 sections. There was no evidence of erosion, ulceration, inflammation, or infectious agents, but algae and diatoms were observed. Six biopsies yielded aerobic isolates (Chryseobacterium indologenes, Aeromonas hydrophila, Ralstonia pickettii, and Morganella morganii), whereas 11 shell samples grew various clostridial anerobes. No fungal organisms were cultured. Although the etiology of the lesions described remains unknown, the use of a scoring system in conjunction with full thickness biopsies is suggested to help standardize investigations into chelonian shell disease in the future.
Assuntos
Biópsia/veterinária , Osso e Ossos/anormalidades , Osso e Ossos/patologia , Tartarugas/anormalidades , Animais , Animais de Zoológico , Infecções Bacterianas/complicações , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/patologia , Infecções Bacterianas/veterinária , Biópsia/métodos , Osso e Ossos/ultraestrutura , Feminino , Imuno-Histoquímica/veterinária , MasculinoRESUMO
Currently used dosages for external-beam megavoltage radiation therapy in birds have been extrapolated from mammalian patients and often appear to provide inadequate doses of radiation for effective tumor control. To determine the tolerance doses of cutaneous and mucosal tissues of normal birds in order to provide more effective radiation treatment for tumors that have been shown to be radiation responsive in other species, ingluvial mucosa and the skin over the ingluvies of 9 ring-necked parakeets (Psittacula krameri) were irradiated in 4-Gy fractions to a total dose of either 48, 60, or 72 Gy using an isocentric cobalt-60 teletherapy unit. Minimal radiation-induced epidermal changes were present in the high-dose group histologically. Neither dose-related acute nor chronic radiation effects could be detected in any group grossly in cutaneous or mucosal tissue over a 9-month period. Radiation doses of 72 Gy in 4-Gy fractions were well tolerated in the small number of ring-necked parakeets in this initial tolerance dose study.
Assuntos
Psittacula , Lesões por Radiação/veterinária , Radioterapia de Alta Energia/veterinária , Animais , Doenças das Aves , Relação Dose-Resposta à Radiação , Feminino , Masculino , Dosagem Radioterapêutica/veterinária , Radioterapia de Alta Energia/efeitos adversos , DermatopatiasRESUMO
Repeated polymerase chain reaction (PCR) testing of 3 asymptomatic domestic cats were positive for Cytauxzoon felis DNA, suggesting persistent infection. Two cats initially presented with clinical signs consistent with acute cytauxzoonosis and, in both cases, signs of illness resolved after treatment. Parasitemia was detected in peripheral blood smears from these cats upon presentation with illness and, at subsequent follow-up appointments, in the absence of clinical illness. Polymerase chain reaction analysis was positive for C. felis from blood sampled at each time point. A third cat, a housemate of a cat fatally infected with C. felis, was preventatively treated for infection at the time of the housemate cat's death. This contact cat, having never shown signs of clinical illness consistent with cytauxzoonosis infection, had no detectable parasitemia but was positive for C. felis on repeated PCR testing. Detection of asymptomatically infected cats allows for the possibility of a yet unrecognized population of infected domestic cats that may have the capacity to serve as an additional reservoir host for C. felis, altering the currently accepted paradigm of C. felis transmission to domestic cats through bobcats as the reservoir host. In cases of very low parasitemia, more sensitive means of parasite detection, such as PCR testing, may be necessary to detect infected cats. Increased detection of asymptomatically infected cats will aid in understanding the epidemiology of C. felis infection and enhance the ability to prevent this highly fatal infectious disease of domestic cats.
Assuntos
Doenças do Gato/diagnóstico , Reação em Cadeia da Polimerase/veterinária , Infecções Protozoárias em Animais/diagnóstico , Animais , Antiprotozoários/uso terapêutico , Doenças do Gato/tratamento farmacológico , Gatos , Feminino , Imidocarbo/uso terapêutico , Masculino , Parasitemia , Infecções Protozoárias em Animais/sangue , Infecções Protozoárias em Animais/tratamento farmacológicoRESUMO
OBJECTIVE: To evaluate endoscopic liver biopsy and compare that technique with a standard coeliotomy biopsy technique in fish. DESIGN: Randomized controlled clinical trial. ANIMALS: 30 channel catfish (Ictalurus punctatus). PROCEDURES: 10 fish were randomly assigned into control, coeliotomy, and coelioscopy groups. Anesthesia was performed with a recirculating anesthesia machine. Body weight, PCV, and total protein (TP) concentration in blood as well as plasma activities of aspartate aminotransferase, creatinine phosphokinase, lactate dehydrogenase, and sorbitol dehydrogenase were measured before and after surgery. Standard ventral coeliotomy or coelioscopy was performed, and the biopsy specimens were scored histologically. RESULTS: Coeliotomy and coelioscopy procedures were well tolerated without acute deaths. Blood TP concentration and PCV decreased after surgery in the coelioscopy group because of intracoelomic fluid administration to aid visualization. Minor changes in activities for hepatic and muscular enzyme activities were apparent, but were not significantly different between the coelioscopy and coeliotomy groups. Coelioscopy and coeliotomy yielded biopsy specimens of similar diagnostic quality. However, coelioscopy permitted a more extensive evaluation of the viscera, and all 10 surgical wounds healed completely, compared with severe wound dehiscence in 3 of 10 fish that underwent coeliotomy. CONCLUSIONS AND CLINICAL RELEVANCE: Both coelioscopy and coeliotomy were capable of yielding antemortem liver biopsy specimens of diagnostic quality in catfish. Coelioscopy permitted a more detailed examination of the coelomic viscera through a smaller surgical incision, was less traumatic, and resulted in decreased wound dehiscence.
Assuntos
Biópsia/veterinária , Ictaluridae , Laparoscopia/veterinária , Laparotomia/veterinária , Fígado/patologia , Cicatrização/fisiologia , Animais , Biópsia/métodos , Proteínas Sanguíneas/análise , Doenças dos Peixes/diagnóstico , Doenças dos Peixes/patologia , Ictaluridae/cirurgia , Laparoscopia/métodos , Laparotomia/métodos , Fígado/enzimologia , Hepatopatias/diagnóstico , Hepatopatias/patologia , Hepatopatias/veterináriaRESUMO
Turtle populations are decreasing dramatically due to habitat loss and collection for the food and pet market. This study sought to determine hematologic values in two species of turtles to help assess health status of captive and wild populations. Blood samples were collected from 12 individuals of the Pascagoula map turtle (Graptemys gibbonsi) and seven individuals of the southeast Asian box turtle (Cuora amboinensis) from the Savannah River Ecology Laboratory (South Carolina, USA). The hematologic data included hematocrit, total solids, erythrocyte count, leukocyte count, and differential and percentage leukocyte counts. Low hematocrit values and high basophil counts were found in both species. The basophil was the most abundant leukocyte in the Pascagoula map turtle (median = 0.80 x 10(9)/L), whereas in the Southeast Asian box turtle the most abundant leukocyte was the heterophil (median = 2.06 x 10(9)/L).
Assuntos
Testes Hematológicos/veterinária , Tartarugas/sangue , Animais , Animais Selvagens/sangue , Animais de Zoológico/sangue , Contagem de Células Sanguíneas/veterinária , Feminino , Hematócrito/veterinária , Masculino , Valores de Referência , Especificidade da EspécieRESUMO
A 1.5-year-old male Moluccan cockatoo (Cacatua moluccensis) presented to Kansas State University Veterinary Medical Teaching Hospital with a 4-month history of lethargy and weakness. Hematologic and radiographic diagnostic testing revealed profound leukocytosis and coelomic and pulmonary granulomatous masses of unknown origin. The bird died during laparoscopic evaluation under general anesthesia. Necropsy revealed multiple pulmonary and hepatic soft-tissue nodules and an intracoelomic mass over the left kidney communicating with external subcutaneous masses and a pericloacal mass of similar gross appearance. Histopathologic findings identified a severe, disseminated, inflammatory infiltration of multiple tissues and multiple granulomas containing bizarre multinucleated cells. No causative agent of this granulomatous disease was identified. To our knowledge, this is the first report of systemic atypical granulomatous disease in Moluccan cockatoo. Traditional causes of granulomatous disease include mycotic disease, bacterial (ie, Mycobacterium) disease, and neoplasia. Attempts to identify an causative agent or neoplasia were unsuccessful. A retrospective review of pathology records revealed 2 additional cases with identical pathologic lesions. All 3 cases occurred in young Moluccan cockatoos and are assumed to be a disease of unknown origin that could be unique to this species.
Assuntos
Doenças das Aves/patologia , Cacatuas , Transtornos Linfoproliferativos/veterinária , Animais , Trato Gastrointestinal/patologia , Fígado/patologia , Pulmão/patologia , Transtornos Linfoproliferativos/patologia , MasculinoRESUMO
Cytology is a useful, rapid, inexpensive diagnostic technique that is particularly suitable for ferrets because of their small size and readily accessible organs and tissues. This article begins with a brief discussion of general cytologic information. The remainder of the article concentrates on the cytologic features of common diseases that affect ferrets.
Assuntos
Citodiagnóstico/veterinária , Furões , Animais , Infecções/diagnóstico , Infecções/veterinária , Inflamação/diagnóstico , Inflamação/veterinária , Fígado/patologia , Linfonodos/patologia , Neoplasias/diagnóstico , Neoplasias/veterinária , Sistema Respiratório/patologia , Dermatopatias/diagnóstico , Dermatopatias/veterinária , Baço/patologiaRESUMO
An overview of avian cytology is presented, discussing more common abnormalities that are encountered in routine clinical practice. The general cytologic features of inflammatory, infectious, and neo-plastic lesions are described. The remainder of the article covers major cytologic abnormalities by anatomic site of origin of the specimens.
Assuntos
Doenças das Aves/patologia , Técnicas Citológicas/veterinária , Infecções/veterinária , Inflamação/veterinária , Neoplasias/veterinária , Animais , Aves , Infecções/patologia , Inflamação/patologia , Neoplasias/patologiaRESUMO
Intracytoplasmic inclusion bodies suggestive of iridovirus infection were observed in formalin-fixed, paraffin-embedded tissues from a nautilus (Nautilus spp.) that died without premonitory signs. Transmission electron microscopy revealed enveloped, hexagonal, viral particles that measured approximately 176 nm in diameter. Virions contained a dense central core and morphology typical of iridoviruses. Extracted DNA was amplified using primers homologous to conserved iridovirus sequences. The amplicons were cloned, sequenced, and determined to be approximately 60% similar to reported amphibian iridovirus sequences. A polymerase chain reaction-generated digoxigenin probe was used to detect viral nucleic acid in tissue sections by DNA in situ hybridization and high-affinity cytochemistry. The detected nucleic acid corresponded to the inclusion bodies observed microscopically. This represents a novel iridovirus of mollusks.
Assuntos
Infecções por Vírus de DNA/veterinária , Iridovirus/crescimento & desenvolvimento , Nautilus/virologia , Animais , Sequência de Bases , Infecções por Vírus de DNA/virologia , DNA Viral/química , DNA Viral/genética , Iridovirus/genética , Rim/ultraestrutura , Rim/virologia , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Nautilus/ultraestrutura , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
Four female and 3 male Taita falcons (Falco fasciinucha) out of a breeding colony of 14 Taita falcons (7 pairs) died during the breeding season after showing lethargy and anorexia for 1 to 2 days. All animals were submitted for necropsy. Gross lesions in the female falcons were characterized by anemia secondary to marked hemorrhage into the ovary and oviduct, serofibrinous effusion into the cardioabdominal cavity and serosal petechiae. In addition, marked necrotizing splenitis and pulmonary hemorrhage were present. Histologically, the female falcons had mild necrotizing hepatitis with numerous intranuclear inclusion bodies and necrotizing splenitis with rare inclusion bodies. There were no gross lesions in the male falcons, and the histological lesions were characterized by urate deposition and rare intranuclear inclusion bodies in the renal tubular epithelial cells. Adenoviral particles were found by electron microscopy in the cloacal contents of the female Taita falcons but not in the male falcons. DNA in situ hybridization revealed widespread aviadenoviral nucleic acid within the nuclei of hepatocytes, renal tubular epithelial cells, and adrenal cells in the female falcons but no aviadenoviral nucleic acid in 1 male falcon and only a low quantity of adenoviral nucleic acid in the liver and kidney of another male Taita falcon. PCR amplified aviadenoviral DNA in the liver and intestine of all Taita falcons. The amplicons were sequenced, and the virus was identified as falcon adenovirus. The deaths of the female and male birds were attributed to the aviadenovirus infection.
Assuntos
Infecções por Adenoviridae/veterinária , Aviadenovirus/isolamento & purificação , Doenças das Aves/virologia , Surtos de Doenças/veterinária , Falconiformes , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/patologia , Infecções por Adenoviridae/virologia , Animais , Aviadenovirus/genética , Doenças das Aves/epidemiologia , Doenças das Aves/patologia , DNA Viral/química , DNA Viral/genética , Feminino , Histocitoquímica/veterinária , Fígado/patologia , Fígado/ultraestrutura , Fígado/virologia , Masculino , Microscopia Eletrônica de Transmissão , Reação em Cadeia da Polimerase/veterinária , Baço/patologia , Baço/ultraestrutura , Baço/virologiaAssuntos
Doenças do Gato/microbiologia , Celulite (Flegmão)/veterinária , Infecções por Mycobacterium não Tuberculosas/veterinária , Mycobacterium fortuitum/isolamento & purificação , Dermatopatias Bacterianas/veterinária , Animais , Doenças do Gato/patologia , Gatos , Celulite (Flegmão)/microbiologia , Celulite (Flegmão)/patologia , Feminino , Infecções por Mycobacterium não Tuberculosas/microbiologia , Infecções por Mycobacterium não Tuberculosas/patologia , Dermatopatias Bacterianas/microbiologia , Dermatopatias Bacterianas/patologiaRESUMO
Stomatitis is a common problem in lizards, and the etiologies of stomatitis in lizards are not well understood. Four green tree monitor lizards (Varanus prasinus) from two different collections were evaluated because of proliferative stomatitis. Degenerate PCR primers targeting a conserved region of herpesvirus DNA-dependent DNA polymerase were used to amplify and sequence a product from gingival tissue of three of four lizards (cases 1, 3, and 4). DNA in situ hybridization of tissues from three lizards was positive for herpesvirus in the oral mucosa of all three lizards tested (cases 1-3) and the brain of two lizards (cases 1 and 3). Comparative sequence analysis suggests that this virus is a novel member of the subfamily alpha-herpesvirinae, and is here termed varanid herpesvirus 1.
Assuntos
Infecções por Herpesviridae/veterinária , Herpesviridae/crescimento & desenvolvimento , Lagartos/virologia , Estomatite Herpética/veterinária , Animais , Sequência de Bases , DNA Viral/química , DNA Viral/genética , Feminino , Herpesviridae/genética , Infecções por Herpesviridae/patologia , Infecções por Herpesviridae/virologia , Dados de Sequência Molecular , Mucosa Bucal/virologia , Hibridização de Ácido Nucleico , Filogenia , Reação em Cadeia da Polimerase/veterinária , Estomatite Herpética/patologia , Estomatite Herpética/virologiaRESUMO
A 2-year-old domestic ferret that appeared clinically healthy was repeatedly seropositive for Aleutian mink disease parvovirus (ADV) over a 2-year observation period. Antibody titers, determined by counter-immunoelectrophoresis, ranged from 1024 to 4096. Viral DNA also was identified in serum, urine, feces, and blood cell fractions by polymerase chain reaction analysis. Ultimately, DNA in situ hybridization revealed ADV DNA in histologic sections of various tissues and organs. These data indicate that this asymptomatic ferret was persistently infected with ADV.