RESUMO
A Gram-stain-negative, aerobic bacterial strain, designated Y21T, was isolated from surface lake water in Xiamen, Fujian Province of China. Growth was observed at temperatures from 4 to 37 °C, at salinities from 0 to 7.0 % and at pH from 6.0 to 10.0. Optimum growth was observed at 28 °C, at pH 7.0 and with 1.5-2.0 % (w/v) NaCl. The highest similarity of 16S rRNA gene sequence between strain Y21T and the other strains was 96.9 %. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain was a member of the genus Rhizobium, forming a distinct lineage with R. subbaraonis KCTC 23614T. The dominant fatty acids were summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), C18:1 ω7c 11-methyl, which accounted for 78.1 %. The G+C content of the chromosomal DNA was 60.9 mol%. The predominant respiratory quinone was ubiquinone-10. The polar lipids of strain Y21T were found to consist of five unidentified phospholipids and three unidentified aminolipids. According to its morphology, physiology, fatty acid composition and 16S rRNA sequence data, strain Y21T should be regarded as a new species of the genus Rhizobium, for which Rhizobium albus sp. nov. is proposed (type strain Y21T = MCCC 1F01210T = KCTC 42252T).
Assuntos
Lagos/microbiologia , Rhizobium/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Lagos/análise , Filogenia , RNA Ribossômico 16S/genética , Rhizobium/classificação , Rhizobium/genética , Rhizobium/metabolismoRESUMO
Alexandrium tamarense is a toxic harmful algal blooms (HABs) causing species, which poses great threat to human health and marine economy. In this study, we isolated an algicidal bacterium Altererythrobacter sp. LY02 towards to A. tamarense and later investigated the algicidal activity, algicidal mode, characteristics of algicidal active substance and algicidal procedure. The results indicated that the cell-free filtrate of strain LY02 showed high algicidal effect on algal growth, however, bacterial cells almost lost algicidal activity. The algicidal active substance was temperature- and pH-stability, and its molecular weight was less than 1000 Da, and was a non-proteinaceous material or non-polysaccharide, mid-polar substance. Under the algicidal effect of active substance, the morphology and structure of A. tamarense cells were seriously damaged as well as organelles. Our study confirmed that the algicidal active substance could be used as an excellent bio-agent for controlling HABs caused by A. tamarense.
Assuntos
Alphaproteobacteria/química , Antiprotozoários/toxicidade , Dinoflagellida/efeitos dos fármacos , Proliferação Nociva de Algas/efeitos dos fármacos , Alphaproteobacteria/genética , Alphaproteobacteria/isolamento & purificação , Alphaproteobacteria/metabolismo , Antiprotozoários/química , Antiprotozoários/metabolismo , Dinoflagellida/crescimento & desenvolvimento , Estabilidade de Medicamentos , Concentração de Íons de HidrogênioRESUMO
A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, yellow bacterium designated strain KD53(T), was isolated from a culture of the alga Phaeodactylum tricornutum from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD53(T) was a member of the Roseobacter clade within the family Rhodobacteraceae , forming a distinct lineage with species of the genus Mameliella . The 16S rRNA gene sequence similarities between strain KD53(T) and other strains examined were all less than 97.0%. Strain KD53(T) was found to grow optimally at 28 °C, at pH 7.5-8.0 and in the presence of 3% (w/v) NaCl. The dominant fatty acids of strain KD53(T) were C18 : 1ω6c and/or C18 : 1ω7c, C18 : 0 and C16 : 0. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 65 mol% and the major respiratory quinone was ubiquinone 10 (Q-10). On the basis of phenotypic data and phylogenetic inference, strain KD53(T) represents a novel member of the genus Mameliella , then the name Mameliella phaeodactyli sp. nov. is proposed. The type strain is KD53(T) (â=MCCC 1K00273(T)â=KCTC 42178(T)).
Assuntos
Diatomáceas/microbiologia , Filogenia , Rhodobacteraceae/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodobacteraceae/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A Gram-staining-negative, orange-pigmented, non-motile, aerobic bacterial strain, designated GYP20T, was isolated from a culture of the alga Picochlorum sp., a promising feedstock for biodiesel production, which was isolated from the India Ocean. Growth was observed at temperatures from 20 to 37 °C, salinities from 0 to 3% and pH from 5 to 9.Mg2+ and Ca2+ ions were required for growth. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain was a member of the genus Phaeodactylibacter, which belongs to the family Saprospiraceae. Strain GYP20T was most closely related to Phaeodactylibacter xiamenensis KD52T (95.5% sequence similarity). The major fatty acids were iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3. The predominant respiratory quinone was menaquinone-7 (MK-7). The polar lipids of strain GYP20T were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified glycolipids, two unidentified phospholipids and three unidentified aminolipids. According to its morphology, physiology, fatty acid composition and 16S rRNA sequence data, the novel strain most appropriately belongs to the genus Phaeodactylibacter, but can readily be distinguished from Phaeodactylibacter xiamenensis GYP20T. The name Phaeodactylibacter luteus sp. nov. is proposed with the type strain GYP20T ( = MCCC 1F01222T = KCTC 42180T).
Assuntos
Bacteroidetes/classificação , Clorófitas/microbiologia , Filogenia , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Oceano Índico , Dados de Sequência Molecular , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A Gram-staining-negative, orange-pigmented, aerobic bacterial strain, designated KA37T, was isolated from a mangrove sediment sample collected from Yunxiao mangrove National Nature Reserve, Fujian Province, China. Growth was observed at 4-37 °C, 0-3% (w/v) NaCl and pH 5-10. Mg2+ ions were required for growth. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus Erythrobacter, which belongs to the family Erythrobacteraceae. Strain KA37T was most closely related to Erythrobacter gangjinensis KCTC 22330T (96.9% sequence similarity), followed by Erythrobacter marinus KCTC 23554T (96.8%); similarity to other members of the genus was below 96.6%. The major fatty acids were C17 : 1ω6c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). Strain KA37T did not produce bacteriochlorophyll a. The predominant respiratory quinone was ubiquinone 10 (Q-10). The polar lipids of strain KA37T were sphingoglycolipid, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, five unknown lipids and one unidentified phospholipid. According to its morphology, physiology, fatty acid composition and 16S rRNA sequence, the novel strain most appropriately belongs to the genus Erythrobacter, but can be distinguished readily from species of the genus Erythrobacter with validly published names. The name Erythrobacter luteus sp. nov. is proposed, with strain KA37T ( = MCCC 1F01227T = KCTC 42179T) as the type strain.
Assuntos
Sedimentos Geológicos/microbiologia , Filogenia , Sphingomonadaceae/classificação , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Sphingomonadaceae/genética , Sphingomonadaceae/isolamento & purificação , Ubiquinona/químicaRESUMO
A Gram-stain-negative, yellow-pigmented, aerobic bacterial strain, designated LY02(T), was isolated from red tide seawater in Xiamen, Fujian Province, China. Growth was observed at temperatures from 4 to 44 °C, at salinities from 0 to 9% and at pH from 6 to 10. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus Altererythrobacter, which belongs to the family Erythrobacteraceae. Strain LY02(T) was related most closely to Altererythrobacter marensis MSW-14(T) (97.2% 16S rRNA gene sequence similarity), followed by Altererythrobacter ishigakiensis JPCCMB0017(T) (97.1%), Altererythrobacter epoxidivorans JCS350(T) (97.1%) and Altererythrobacter luteolus SW-109(T) (97.0%). The dominant fatty acids were C(18 : 1)ω7c, C(17 : 1)ω6c and summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c). DNA-DNA hybridization showed that strain LY02(T) possessed low DNA-DNA relatedness to A. marensis MSW-14(T), A. ishigakiensis JPCCMB0017(T), A. epoxidivorans JCS350(T) and A. luteolus SW-109(T) (mean ± SD of 33.2 ± 1.3, 32.1 ± 1.0, 26.7 ± 0.7 and 25.2 ± 1.1â%, respectively). The G+C content of the chromosomal DNA was 61.2 mol%. The predominant respiratory quinone was ubiquinone-10 (Q-10). According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, the novel strain most appropriately belongs to the genus Altererythrobacter, but can readily be distinguished from recognized species. The name Altererythrobacter xiamenensis sp. nov. is proposed (type strain LY02(T)â=âCGMCC 1.12494(T)â=âKCTC 32398(T)â=âNBRC 109638(T)).
Assuntos
Alphaproteobacteria/classificação , Proliferação Nociva de Algas , Filogenia , Água do Mar/microbiologia , Alphaproteobacteria/genética , Alphaproteobacteria/isolamento & purificação , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A novel Gram-stain-negative, horseshoe-shaped, non-motile bacterium, designated strain KD51(T), forming colonies coloured pink by carotenoid pigments, was isolated from aggregates of the alga Chlorella autotrophica collected from the coastal sea off the city of Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD51(T) was a member of the genus Cyclobacterium, forming a distinct lineage with Cyclobacterium lianum HY9(T). The 16S rRNA gene sequence similarity between strain KD51(T) and the type strains of species of the genus Cyclobacterium ranged from 92.1â% to 95.2â%. Growth occurred at 4-40 °C (optimum, 28 °C), in the presence of 3-9â% NaCl (optimum, 3-5â%) and at pH 6-10 (optimum, pH 7.5). The dominant fatty acids (>20â%) of strain KD51(T) were iso-C15â:â0 (32.2â%) and summed feature 3 (comprising C16â:â1ω7c and/or C16â:â1ω6c; 22.2â%). The DNA G+C content was 41.7 mol% and the only respiratory quinone was menaquinone-7. On the basis of phenotypic data and phylogenetic inference, strain KD51(T) represents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium xiamenense sp. nov. is proposed. The type strain is KD51(T) (â=âCGMCC 1.12432(T)â=âKCTC 32253(T)). An emended description of the genus Cyclobacterium is also proposed.
Assuntos
Bacteroidetes/classificação , Chlorella/microbiologia , Filogenia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , Carotenoides/química , China , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Pigmentação , RNA Ribossômico 16S/genética , Água do Mar , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A Gram-stain-negative, elongated rod-shaped, motile by gliding, green-pigmented, aerobic bacterial strain, designated LY03(T), was isolated from lake water in Xiamen, Fujian Province, China. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus Chitinimonas, which belongs to the family Burkholderiaceae. Strain LY03(T) was most closely related to Chitinimonas taiwanensis LMG 22011(T) (96.02â% 16S rRNA gene sequence similarity), followed by Chitinimonas koreensis KACC 11467(T) (94.85â%), and the three strains formed a distinct lineage from other strains in the phylogenetic analyses. Optimum conditions for growth were 37 °C, pH 7-9 and without NaCl. The major fatty acids were summed feature 3 (C16â:â1ω6c and/or C16â:â1ω7c), C16â:â0 and C10â:â0 3-OH. The DNA G+C content of strain LY03(T) was 63.6 mol% and the major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown polar lipids and unidentified phospholipids. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain LY03(T) from all other members of the genus Chitinimonas. On the basis of its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain LY03(T) represents a novel species of the genus Chitinimonas, for which the name Chitinimonas prasina sp. nov. is proposed. The type strain is LY03(T) (â=âMCCC 1F01209(T)â=âKCTC 32574(T)).
Assuntos
Burkholderiaceae/classificação , Lagos/microbiologia , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , Burkholderiaceae/genética , Burkholderiaceae/isolamento & purificação , China , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, reddish-orange and chemoheterotrophic bacteria, designated strain KD52(T), was isolated from a culture of the alga Phaeodactylum tricornutum from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD52(T) was a member of the family Saprospiraceae, forming a distinct lineage with 'Portibacter lacus' KCTC 23747. The 16S rRNA gene sequence similarity between strain KD52(T) and the type strains of species of the family Saprospiraceae ranged from 86% to 89%. Growth occurred at 20-37 °C (optimum, 28 °C), in the presence of 1-9% (w/v) NaCl (optimum, 2.5%) and at pH 5-8.5 (optimum, pH 6.0). The dominant fatty acids (>10%) of strain KD52(T) were iso-C15:0 (33.1%), iso-C15:1 G (14.8%) and summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c, 13.8%). The major polar lipids were diphosphatidylglycerol, three unidentified phospholipids, four unknown lipids and one unidentified aminolipid. The DNA G+C content was 51 mol% and the major respiratory quinone was menaquinone-7 (MK-7). On the basis of phenotypic data and phylogenetic inference, strain KD52(T) represents a novel species of a new genus, for which the name Phaeodactylibacter xiamenensis gen. nov., sp. nov., is proposed. The type strain is KD52(T) ( = MCCC 1F01213(T)â=âKCTC 32575(T)).
Assuntos
Bacteroidetes/classificação , Diatomáceas/microbiologia , Filogenia , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
Alexandrium tamarense is a notorious harmful algal bloom species, which is associated with the largest number of paralytic shellfish poisoning cases, causing devastating economic losses and health hazards. The marine bacterium Mangrovimonas yunxiaonensis strain LY01 showed high algicidal effects on A. tamarense. A. tamarense was also susceptible to the supernatant of LY01 as revealed by algicidal activity assay, but washed bacterial cells did not show algicidal activity towards A. tamarense. In this study, we investigated the algicidal effect of the supernatant on growth, photosynthesis and the antioxidative response of A. tamarense. The results indicated that under the algicidal effect of the supernatant, the contents of cellular pigments including chlorophyll a and carotenoids were significantly decreased, and the decline of the maximum quantum yield and relative electron transport rate values suggested that photosynthetic inhibition occurred in the photosystem II system. The content of reactive oxygen species (ROS) increased after 0.5 h exposure, and the surplus ROS induced lipid peroxidation, the destruction of cellular membrane integrity and decreased cellular protein and carbohydrate contents in the algal cells. At the same time, the supernatant also induced the responses of antioxidant enzymes and non-enzymatic antioxidant. The transcription of photosynthesis- and respiration-related genes were significantly inhibited during the exposure procedure, which obstructed photosynthetic efficiency and capacity and disturbed the respiratory system, thereby increasing ROS production again. All these results elaborate clearly the entire procedure by which cellular physiological levels respond to the algicidal bacterium and may contribute to a better understanding of the bacterial control of A. tamarense.
Assuntos
Alveolados/efeitos dos fármacos , Alveolados/fisiologia , Antibiose , Citotoxinas/metabolismo , Flavobacteriaceae/fisiologia , Estresse Oxidativo , Fotossíntese/efeitos dos fármacos , Alveolados/química , Organismos Aquáticos/química , Organismos Aquáticos/efeitos dos fármacos , Organismos Aquáticos/metabolismo , Organismos Aquáticos/fisiologia , Carboidratos/análise , Carotenoides/análise , Sobrevivência Celular/efeitos dos fármacos , Clorofila/análise , Clorofila A , Flavobacteriaceae/metabolismo , Peroxidação de Lipídeos , Redes e Vias Metabólicas/efeitos dos fármacos , Proteínas de Protozoários/análise , Espécies Reativas de Oxigênio/análiseRESUMO
A Gram-stain negative, short rod-shaped aerobic bacterium with flagella, designated strain Y32(T), was isolated from coastal seawater in Xiamen, Fujian Province of China. 16S rRNA gene sequence comparisons showed that strain Y32(T) is a member of the family Oceanospirillaceae, forming a distinct lineage with species of the genus Litoribacillus. The 16S rRNA gene sequence similarities between strain Y32(T) and other strains were all less than 94.0 %. Strain Y32(T) was found to grow optimally at 28 °C, at pH 7.0-8.0 and in the presence of 4-5 % (w/v) NaCl. The major fatty acids were identified as Summed Feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c, 49.4 %), C16:0 (17.7 %), C14:0 (6.9 %) and C18:1 ω9c (5.4 %). The major respiratory quinone was identified as ubiquinone-8 (Q-8). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain Y32(T) was determined to be 55.6 mol%. According to its morphology, physiology, fatty acid composition, polar lipids composition and 16S rRNA gene sequence data, strain Y32(T) represents a novel species of a new genus in the family Oceanospirillaceae, for which the name Litoribrevibacter albus gen. nov. sp. nov. is proposed. The type strain of Litoribrevibacter albus is Y32(T) (=MCCC 1F01211(T)=NBRC 110071(T)).
Assuntos
Oceanospirillaceae/classificação , Oceanospirillaceae/isolamento & purificação , Água do Mar/microbiologia , Aerobiose , Técnicas de Tipagem Bacteriana , Composição de Bases , China , Análise por Conglomerados , Citosol/química , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Concentração de Íons de Hidrogênio , Locomoção , Dados de Sequência Molecular , Oceanospirillaceae/genética , Oceanospirillaceae/fisiologia , Fosfolipídeos/análise , Filogenia , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Cloreto de Sódio/metabolismo , TemperaturaRESUMO
Phaeocystis globosa causes severe marine pollution by forming harmful algal blooms and releasing hemolytic toxins and is therefore harmful to marine ecosystems and aquaculture industries. In this study, Microbulbifer sp. YX04 exerted high algicidal activity against P. globosa by producing and secreting metabolites. The algicidal activity of the YX04 supernatant was stable after exposure to different temperatures (-80 to 100°C) and pH values (4 to 12) for 2 h, suggesting that algicidal substances could temporarily be stored under these temperature and pH value conditions. To explore the algicidal process and mechanism, morphological and structural changes, oxidative stress, photosynthesis, autophagic flux, and global gene expression were investigated. Biochemical analyses showed that the YX04 supernatant induced reactive oxygen species (ROS) overproduction, which caused lipid peroxidation and malondialdehyde (MDA) accumulation in P. globosa. Transmission electron microscopy (TEM) observation and the significant decrease in both maximum photochemical quantum yield (Fv/Fm) and relative electron transfer rate (rETR) indicated damage to thylakoid membranes and destruction of photosynthetic system function. Immunofluorescence, immunoblot, and TEM analyses indicated that cellular damage caused autophagosome formation and triggered large-scale autophagic flux in P. globosa. Transcriptome analysis revealed many P. globosa genes that were differentially expressed in response to YX04 stress, most of which were involved in photosynthesis, respiration, cytoskeleton, microtubule, and autophagosome formation and fusion processes, which may trigger autophagic cell death. In addition to P. globosa, the YX04 supernatant showed high algicidal activity against Thalassiosira pseudonana, Thalassiosira weissflogii, Skeletonema costatum, Heterosigma akashiwo, and Prorocentrum donghaiense. This study highlights multiple mechanisms underlying YX04 supernatant toxicity toward P. globosa and its potential for controlling the occurrence of harmful algal blooms. IMPORTANCEP. globosa is one of the most notorious harmful algal bloom (HAB)-causing species, which can secrete hemolytic toxins, frequently cause serious ecological pollution, and pose a health hazard to animals and humans. Hence, screening for bacteria with high algicidal activity against P. globosa and studies on the algicidal characteristics and mechanism will contribute to providing an ecofriendly microorganism-controlling agent for preventing the occurrence of algal blooms and reducing the harm of algal blooms to the environment. Our study first reported the algicidal characteristic and mechanism of Microbulbifer sp. YX04 against P. globosa and demonstrated that P. globosa shows different response mechanisms, including movement ability, antioxidative systems, photosynthetic systems, gene expression, and cell death mode, to adapt to the adverse environment when algicidal compounds are present.
Assuntos
Morte Celular Autofágica , Gammaproteobacteria/química , Haptófitas/citologia , Haptófitas/efeitos dos fármacos , Herbicidas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Gammaproteobacteria/metabolismo , Haptófitas/crescimento & desenvolvimento , Haptófitas/metabolismo , Proliferação Nociva de Algas , Herbicidas/química , Herbicidas/metabolismo , Herbicidas/farmacologia , Concentração de Íons de Hidrogênio , Fotossíntese/efeitos dos fármacos , Espécies Reativas de OxigênioRESUMO
NiFe2O4 has been regarded as one of the promising candidates for lithium-ion battery (LIB) anode materials due to its high theoretical specific capacity. However, the large volume expansion and pulverization of NiFe2O4 during the charge/discharge process result in severe capacity fading. Herein, heterostructured NiFe2O4-C nanorods have been successfully fabricated by recovering transition metals from simulated electroplating sludge leaching solution. The constructed NiFe2O4-C heterointerface plays a vital role in accommodating volume change, stabilizing the reaction products and providing rapid electron and Li+ ion transportation ability, resulting in a high and stable Li+ accommodation performance. The fabricated NiFe2O4-C nanorods demonstrate a high specific capacity (889.9 mA h g-1 at 100 mA g-1), impressive rate capability (861.5, 704.5, 651.4, 579.6 and 502.1 mA h g-1 at 0.2, 0.6, 1.0, 2.0 and 5.0 A g-1) and cycling stability (650.2 mA h g-1 at 2 A g-1 after 500 cycles). This work exemplifies a facile and effective approach for the fabrication of high performance LIB electrode materials by recycling metals from electroplating sludge in an application-oriented manner.
RESUMO
In spite of the great potential in leading next-generation energy storage technology, Li-S batteries suffer rapid capacity decay arising from the shuttling effect of lithium polysulfides (LiPSs), a major concern that must be addressed before commercialization can be realized. To tackle this challenge, we demonstrate a facile approach to fabricate a hierarchically structured composite of Fe2P@nitrogen, phosphorus codoped carbon (Fe2P@NPC) by direct biological recycling of iron metal from electroplating sludge using bacteria. This material, featuring uniform dispersion of Fe2P nanoparticles (NPs) in porous NPC matrix, effectively adapts volume variation of sulfur upon cycling and simultaneously provides multiple channels for efficient lithium ion transport. In addition, Fe2P NPs with strong adhesion properties of tightly anchored soluble LiPSs formed during discharge can significantly facilitate the decomposition of Li2S during the subsequent charging process. The Li-S cell built on this cathode architecture delivers high specific capacity (1555.7 mAh g-1 at 0.1 C), appreciable rate capability (679.7 mAh g-1 at 10 C), and greatly enhanced cycling performance (761.9 mAh g-1 at 1.0 C after 500 cycles).
RESUMO
In this study, Marinobacter sp. FL06 was used to effectively harvest the energy-producing microalga Thalassiosira pseudonana through direct flocculation. Strain FL06 showed 92.7% flocculating efficiency against T. pseudonana, and no metal ion was added for the flocculation process, resulting in a more environmentally friendly process. The flocculation efficiency of FL06 was stable over a wide range of pH values and temperatures, indicating that the application of this bacteria has potential advantages under various conditions. Strain FL06 also exhibited flocculation activity against different microalgae, indicating that the strain can be used to harvest multiple types of microalgae. Strain FL06 showed high chemotactic ability toward algal cells, suggesting that chemotaxis is important for flocculation. This study provides the first demonstration that the Marinobacter genus could be used to harvest T. pseudonana biomass. In summary, the results showed that FL06 has the potential for effective harvesting of microalgal biomass.
Assuntos
Marinobacter , Biomassa , Reatores Biológicos , Floculação , Microalgas , TemperaturaRESUMO
To find the potential algicidal microorganisms and apply them to prevent and terminate harmful algal blooms (HABs), we isolated an actinomycete U3 from Mangrove, which had a potent algicidal effect on the harmful alga Heterosigma akashiwo. It could completely lyse the algal cells by producing active compounds, which were highly sensitive to high temperature and strong alkaline, but resistant to acid. One µg/mL of crude extract of the fermentation supernatant could kill 70% of H. akashiwo cells in 3 d. Unlike most of the other known algicidal Streptomyces, U3 showed strong ability of proliferation with the algal inclusion as the nutrient source. The washed mycelial pellets also gradually exhibited significant algicidal effect during the visible growth in the algal culture. It suggests that U3 could efficiently absorb nutrients from algal culture to support its growth and produce algicidal compounds that might cause the autophagy of algal cells. Therefore, applying U3, as a long-term and environmentally friendly bio-agent to control the harmful blooms of H. akashiwo, would be effective and promising. And the decrease of bioavailable DOM and increase of bio-refractory DOM during the algicidal process of U3 provided new insights into the ecological influence of algicial microorganisms on marine ecosystem.
Assuntos
Agentes de Controle Biológico , Proliferação Nociva de Algas , Streptomyces , Microbiologia da ÁguaRESUMO
In recent years, Microcystis aeruginosa blooms have occurred throughout the world, causing huge economic losses and destroying aquatic ecosystems. It is necessary to develop effective and ecofriendly methods to control M. aeruginosa blooms. Here, we report a high algicidal activity of prodigiosin (PG) against M. aeruginosa as well as the algicidal mechanism. PG showed high algicidal activity against M. aeruginosa, with a 50% lethal dose (LD50) of 5.87 µg/mL in 72 h. A combination of methods, including propidium iodide and Annexin V-fluorescein staining assays and light and electron microscopy indicated the existence of two modes of cell death with features similar to those in eukaryotic programmed cell death: necrotic-like and apoptotic-like. Biochemical and physiological analyses showed that PG generates reactive oxygen species (ROS), which induce lipid peroxidation, damage the membrane system and destroy the function of the photosystem. A proteomics analysis revealed that many proteins were differentially expressed in response to PG stress and that most of these proteins were involved in important metabolic processes, which may trigger necrotic-like or apoptotic-like cell death. The present study sheds light on the multiple toxicity mechanisms of PG on M. aeruginosa and its potential for controlling the occurrence of M. aeruginosa blooms in lakes.
Assuntos
Proliferação Nociva de Algas/efeitos dos fármacos , Microcystis/efeitos dos fármacos , Prodigiosina/farmacologia , Apoptose , Gammaproteobacteria/química , Peroxidação de Lipídeos , Microcystis/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteoma/genética , Proteoma/metabolismoRESUMO
Algicidal microbes could effectively remove the harmful algae from the waters. In this study, we were concerned with the ecological influence of an algicide extracted from Streptomyces alboflavus RPS, which could completely lyse the Phaeocystis globosa cells within two days. In microcosms, 4 µg/mL of the microbial algicide could efficiently remove P. globosa cells without suppressing other aquatic organisms. Bioluminescent assays confirmed that the toxicity of microbial algicide at this concentration was negligible. Interestingly, the toxicity of P. globosa exudates was also significantly reduced after being treated with the algicide. Further experiments revealed that the microbial algicide could instantly increase the permeability of the plasma membrane and disturb the photosynthetic system, followed by the deformation of organelles, vacuolization and increasing oxidative stress. The pre-incubation of N-acetyl cysteine (NAC) verified that the rapid damages to the plasma membrane and photosynthetic system caused the algal death in the early phase, and the increasing oxidative stress killed the rest. The late accumulation and possible release of CAT also explained the decreasing toxicity of the algal culture. These results indicated that this microbial algicide has great potential in controlling the growth of P. globosa on site.
Assuntos
Anti-Infecciosos/farmacologia , Haptófitas/efeitos dos fármacos , Streptomyces/metabolismo , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/metabolismo , Catalase/metabolismo , Permeabilidade da Membrana Celular/efeitos dos fármacos , Exsudatos e Transudatos/efeitos dos fármacos , Exsudatos e Transudatos/metabolismo , Citometria de Fluxo , Fluorometria , Haptófitas/metabolismo , Haptófitas/ultraestrutura , Proliferação Nociva de Algas/efeitos dos fármacos , Microscopia Eletrônica de Transmissão , Estresse Oxidativo/efeitos dos fármacos , Oxirredutases/metabolismo , Fotossíntese/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismoRESUMO
Chitinase producing bacteria can involve extensively in nutrient cycling and energy flow in the aquatic environment through degradation and utilization of chitin. It is well known that diatoms cells are encased by box-like frustules composed of chitin. Thus the chitin containing of diatoms shall be a natural target of chitinase producing bacteria, however, the interaction between these two organismic groups has not been studied thus far. Therefore, in this study, the algicidal mechanism of one chitinase producing bacterium (strain LY03) on Thalassiosira pseudonana was investigated. The algicidal range and algicidal mode of strain LY03 were first studied, and then bacterial viability, chemotactic ability and direct interaction characteristic between bacteria and diatom were also confirmed. Finally, the characteristic of the intracellular algicidal substance was identified and the algicidal mechanism was determined whereby algicidal bacterial cells showed chemotaxis to algal cells, fastened themselves on algal cells with their flagella, and then produced chitinase to degrade algal cell walls, and eventually caused algal lysis and death. It is the first time to investigate the interaction between chitinase producing bacteria and diatoms, and this novel special interaction mode was confirmed in this study, which will be helpful in protection and utilization of diatoms resources.