Machine Learning Reveals Serum Glycopatterns as Potential Biomarkers for the Diagnosis of Nonalcoholic Fatty Liver Disease (NAFLD).

Nonalcoholic fatty liver disease (NAFLD) has emerged as the predominant chronic liver condition globally, and underdiagnosis is common, particularly in mild cases, attributed to the asymptomatic nature and traditional ultrasonography's limited sensitivity to detect early-stage steatosis. Consequently, patients may experience progressive liver pathology. The objective of this research is to ascertain the efficacy of serum glycan glycopatterns as a potential diagnostic biomarker, with a particular focus on the disease's early stages. We collected a total of 170 serum samples from volunteers with mild-NAFLD (Mild), severe-NAFLD (Severe), and non-NAFLD (None). Examination via lectin microarrays has uncovered pronounced disparities in serum glycopatterns identified by 19 distinct lectins. Following this, we employed four distinct machine learning algorithms to categorize the None, Mild, and Severe groups, drawing on the alterations observed in serum glycopatterns. The gradient boosting decision tree (GBDT) algorithm outperformed other models in diagnostic accuracy within the validation set, achieving an accuracy rate of 95% in differentiating the None group from the Mild group. Our research indicates that employing lectin microarrays to identify alterations in serum glycopatterns, when integrated with advanced machine learning algorithms, could constitute a promising approach for the diagnosis of NAFLD, with a special emphasis on its early detection.

Emodin as an Inhibitor of PRV Infection In Vitro and In Vivo.

Pseudorabies (PR) is an acute and severe infectious disease caused by pseudorabies virus (PRV). Once the virus infects pigs, it is difficult to eliminate, resulting in major economic losses to the global pig industry. In addition, reports of human infection with PRV suggest that the virus is a potential threat to human health; thus, its significance to public health should be considered. In this paper, the anti-PRV activities of emodin in vitro and in vivo, and its mechanism of action were studied. The results showed that emodin inhibited the proliferation of PRV in PK15 cells in a dose-dependent manner, with an IC50 of 0.127 mg/mL and a selection index of 5.52. The addition of emodin at different stages of viral infection showed that emodin inhibited intracellular replication. Emodin significantly inhibited the expression of the IE180, EP0, UL29, UL44, US6, and UL27 genes of PRV within 48 h. Emodin also significantly inhibited the expression of PRV gB and gD proteins. The molecular docking results suggested that emodin might form hydrogen bonds with PRV gB and gD proteins and affect the structure of viral proteins. Emodin effectively inhibited the apoptosis induced by PRV infection. Moreover, emodin showed a good protective effect on PRV-infected mice. During the experimental period, all the control PRV-infected mice died resulting in a survival rate of 0%, while the survival rate of emodin-treated mice was 28.5%. Emodin also significantly inhibited the replication of PRV in the heart, liver, brain, kidneys and lungs of mice and alleviated tissue and organ damage caused by PRV infection. Emodin was able to combat viral infection by regulating the levels of the cytokines TNF-α, IFN-γ, IL-6, and IL-4 in the sera of infected mice. These results indicate that emodin has good anti-PRV activity in vitro and in vivo, and is expected to be a new agent for the prevention and control of PRV infection.

Turning Complexity into Simplicity: In Situ Synthesis of High-Performance Si@C Anode in Battery Manufacturing Process by Partially Carbonizing the Slurry of Si Nanoparticles and Dual Polymers.

For Si/C anodes, achieving excellent performance with a simple fabrication process is still an ongoing challenge. Herein, we report a green, facile and scalable approach for the in situ synthesis of Si@C anodes during the electrode manufacturing process by partially carbonizing Si nanoparticles (Si NPs) and dual polymers at a relatively low temperature. Due to the proper mass ratio of the two polymer precursors and proper carbonization temperature, the resultant Si-based anode demonstrates a typical Si@C core-shell structure and has strong mechanical properties with the aid of dual-interfacial bonding between the Si NPs core and carbon shell layer, as well as between the C matrix and the underlying Cu foil. Consequently, the resultant Si@C anode shows a high specific capacity (3458.1 mAh g-1 at 0.2 A g-1), good rate capability (1039 mAh g-1 at 4 A g-1) and excellent cyclability (77.94% of capacity retention at a high current density of 1 A g-1 after 200 cycles). More importantly, the synthesis of the Si@C anode is integrated in situ into the electrode manufacturing process and, thus, significantly decreases the cost of the lithium-ion battery but without sacrificing the electrochemical performance of the Si@C anode. Our results provide a new strategy for designing next-generation, high-capacity and cost-effective batteries.

[Risk assessment of lead and cadmium exposure of Nanjing residents through vegetables and edible fungus from 2014 to 2018].

OBJECTIVE: To evaluate the potential risk of lead and cadmium through vegetables and edible fungus in Nanjing City from 2014 to 2018. METHODS: Based on the survey of dietary consumption of residents in Nanjing City, combining with the concentration data of lead and cadmium in vegetables and edible fungus, using @Risk software to simulate and analyze the cumulative dietary exposure in a probabilistic way, and compared with the health guidance value or benchmark dose(BMD) to assess the potential health risks of lead and cadmium for Nanjing residents. RESULTS: The average exposure of lead and cadmium was 0.237 and 0.090 µg/kg, the exposure of cadmium in the general population were lower than the corresponding health guidance value, meanwhile the margin of exposure(MOE) for lead was higher than 1 in 18-49 years old group and over 50 years old group respectively, except for the high-end exposure(P95) in 6-17 years group the MOE for lead was 0.9. The pollution level and the consumption of leafy vegetables, edible fungus and artemisia selengensis were the main factors affecting the exposure of lead and cadmium through vegetables and edible fungus. CONCLUSION: The exposure to lead and cadmium through vegetables is at a relatively safe level, but more attention should be paid to the risk of lead and cadmium exposure in 6-17 years old and over 50 years old group of high-end exposure population.

Halogen-modified carbazole derivatives for lipid droplet-specific bioimaging and two-photon photodynamic therapy.

Lipid droplets (LDs) are dynamic multifunctional organelles that participate in the regulation of many metabolic processes, visualization of which is necessary for biological research. In this work, a series of two-photon responsive fluorescent probes (C-H, C-Br, and C-I) based on carbazole units were designed and synthesized. Thereinto, an iodine-modified carbazole derivative C-I exhibited an exciting lipid droplet targeting ability due to its excellent lipophilicity. Meanwhile, benefiting from its larger Stokes shift and two-photon absorption cross-section, C-I was employed for two-photon confocal laser scanning microscopy (CLSM) and stimulated emission depletion (STED) microscopy imaging to observe LDs more accurately. In addition, given the heavy atom effect, C-I can effectively generate reactive oxygen species (ROS) leading to cancer cell apoptosis under near-infrared light irradiation. Notably, we explained the process of cell apoptosis through in vitro simulation experiments. This study provides a promising platform for visualization of lipid droplets.

Identification of Blood miR-216a, miR-377 and Their Target Genes ANGPTL4, GAP-43 and Serum of PPARG as Biomarkers for Diabetic Peripheral Neuropathy of Type 2 Diabetes.

BACKGROUND: Diabetic peripheral neuropathy (DPN) is one of the most common and complex chronic complications of diabetes, but it is clinically lacking effective means for early diagnosis and early treatment. MicroRNA, in the occurrence and development of the disease, has an important regulatory role. Its role in diabetes has been reported more. However, specific research on microRNA in DPN is rare. METHODS: Based on the results of bioinformatics screening, miR-377 and miR-216a, their respective target molecules growth association protein 43 (GAP-43) and angiopoietin-like 4 protein (ANGPTL4), and related pathways peroxisome proliferator activated receptor gamma (PPARG) and chemerin were tested by RT-qPCR and ELISA in blood samples of DPN to analyze the correlation between these differentially expressed molecules and clinico-pathological factors of DPN. RESULTS: In this study, we found that miR-377, miR-216a, GAP-43, ANGPTL4, and PPARG were significantly differentially expressed genes for DPN. The correlation analysis showed that they were closely related to the clinical indicators of DPN suggesting that they may be involved in the development of DPN. In addition, receiver operating characteristic (ROC) curves generated for miR216a, miR377, ANGPTL4, GAP43, PPARG revealed that they can be used as new molecular diagnostic markers of DPN. CONCLUSIONS: miR-216a, miR-377, ANGPTL4, GAP-43, and PPARG could potentially be biomarkers of DPN.

Association between sleep-disordered breathing and lower extremity artery disease: a meta-analysis.

OBJECTIVE: Previous studies suggest a relationship between sleep disordered breathing (SDB) and lower extremity artery disease (LEAD) though disagreements remain. This study was performed to assess the association between SDB and LEAD in a metaanalysis. METHODS: PubMed and Embase databases were searched for observational studies. A random-effects model incorporating between-study heterogeneity was used to pool the data. RESULTS: The search revealed 7 studies including 152,885 participants. Patients with SDB had higher odds of LEAD (risk ratio [RR] = 1.32, 95% confidence interval [CI] = 1.07 to 1.63, p = 0.01, I 2 = 66%) compared to non-SDB participants. In subgroup analyses, SDB was related to higher odds of LEAD in patients with stroke or diabetes (RR = 3.28, p < 0.001) than in a community-derived population (RR = 1.17, p = 0.08; p for subgroup difference < 0.001). Furthermore, the odds of LEAD was increased in patients with moderate (RR = 1.34, 95% CI = 1.04 to 1.73, p = 0.02) or severe SDB (RR = 1.81, 95% CI = 1.08 to 3.03, p = 0.02), but not in those with mild SDB (RR = 0.94, 95% CI = 0.69 to 1.29, p = 0.71). Differences in study design or methods for LEAD diagnosis did not appear to affect the association between SDB and LEAD. CONCLUSIONS: Patients with SDB have higher odds of LEAD compared to non-SDB participants.

Development and validation of a photographic atlas of food portions for accurate quantification of dietary intakes in China.

BACKGROUND: Accurate estimation of food portion sizes remains an important challenge in dietary data collection. The present study aimed to develop a food atlas with adequate visual reference to improve the accuracy of dietary surveys in China. METHODS: A food atlas for dietary surveys in China was developed using three visual reference systems, namely, regularly placed food portions, the two-dimensional background coordinates and common objects known in daily life. The atlas was validated by estimating a meal before and after using the food atlas, and differences in weight estimation were compared using a paired t-test. In total, 50 college students participated in the study. RESULTS: After determination of food varieties; design of the food display; purchase, processing, cooking and weighing of food; photographing food; post-image processing and data processing, a total of 799 pictures of 303 types of food and two types of tableware were produced. The mean value of food weight estimated with the atlas was closer to the actual weight, and the variation range of these values was smaller and more stable than that estimated without the atlas. The differences estimated before and after using the atlas for all foods were significant (P < 0.05). Comparing the differences in weight before using the atlas, the error ranges of food samples were reduced. CONCLUSIONS: A food atlas has been developed for a retrospective dietary survey in China, which can be used to enable a better understanding of nutritional adequacy in the Chinese population.

Genome-wide identification and expression analysis of the CLC superfamily genes in tea plants (Camellia sinensis).

The voltage-gated chloride channel (CLC) superfamily is one of the most important anion channels that is widely distributed in bacteria and plants. CLC is involved in transporting various anions such as chloride (Cl-) and fluoride (F-) in and out of cells. Although Camellia sinensis is a hyper-accumulated F plant, there is no studies on the CLC gene superfamily in the tea plant. Here, 8 CLC genes were identified from C. sinensis and they were named CsCLC1-8. The structure of CsCLC genes and the proteins were not conserved; the number of exons varied from 3 to 24, and the number of transmembrane domains contained 2 to 10. Furthermore, phylogenetic analysis revealed that CsCLC4-8 in subclass I contained the typical conserved domains GxGIPE (I), GKxGPxxH (II) and PxxGxLF (III), and CsCLC1-3 in subclass II did not contain any of the three conserved residues. We measured the expression levels of CsCLCs in roots, stems and leaves to assess the responses to different concentrations of Cl- and F-. The result indicated that CsCLCs participated in subfunctionalization in response to Cl- and F-, and CsCLC1-3 was more sensitive to F- treatments than CsCLC4-8, CsCLC6 and CsCLC7 may participate in absorption and long-distance transport of Cl-.

pH-triggered morphological change in a self-assembling amphiphilic peptide used as an antitumor drug carrier.

The geometry of nanoparticles plays an important role in the process of drug encapsulation and release. In this study, an acid-responsive amphiphilic polypeptide consisting of lysine and leucine was prepared. In neutral media, the amphiphilic peptide L6K4 self-assembled to form spherical nanoparticles and encapsulated fat-soluble antitumor drugs. The intratumoral accumulation of the drug-loaded nanoparticles was improved in HeLa cells compared with normal cells. Compared to a neutral environment, increasingly acidic solutions changed the secondary structure of the peptide. In addition, the drug-loaded nanoparticles expanded and decomposed, rapidly releasing the poorly soluble antitumor drug doxorubicin (DOX). In addition, the amphiphilic peptide L6K4 had antitumor properties, and the antitumor performance of the combination of L6K4 and DOX was better than that of free DOX. Our results indicate that the use of acid responsiveness to induce geometric changes in drug-loaded peptide nanoparticles could be a promising strategy for antitumor drug delivery.

[Theoretical risk assessment for dietary exposure to sodium saccharin among residents in Nanjing City].

OBJECTIVE: To evaluate the potential risk of dietary exposure to sodium saccharin among residents in Nanjing City, and provide scientific evidence for safety surveillance and risk management in processed foods. METHODS: Based on the survey of dietary consumption of residents in Nanjing City, combining with the limits of sodium saccharin in processed foods(GB 2760-2014), using @Risk software to establish the cumulative dietary exposure in a probabilistic way, and compared with the acceptable daily intake(ADI) for risk assessment. RESULTS: The average exposure of sodium saccharin of residents was 0. 69 mg/kg, accounted for 13. 80% of ADI, while high exposure(P97. 5) was 5. 37 mg/kg, and accounted for 107. 36% of ADI. The exposure of sodium saccharin of residents in all age groups exceeded ADI were ranged from 20. 60% to 132. 80%. The intake of sodium saccharin exposure of high exposures(P97. 5) exceeded ADI was 132. 80% and 110. 75% in 18-49 years and 11-17 years people respectively. Cooked nuts, candied fruit and bean products were the main sources of sodium saccharin exposure compared with others. CONCLUSION: Dietary exposure assessment shows that the risk dietary exposure to sodium saccharin is generally safe. Residents who intake these processed foods higher should be paid more attention to reduce the level of sodium saccharin exposure.

Prognostic Significance of the Lymph Node Ratio in Surgical Patients With Distal Cholangiocarcinoma.

BACKGROUND: The aim of this study was to compare the prognostic impact of the lymph node ratio (LNR) versus positive lymph node count (PLNC) in patients who had undergone resection for distal cholangiocarcinoma. METHODS: We identified 448 patients with resected distal cholangiocarcinoma from the Surveillance, Epidemiology, and End Results database. The X-Tile program was used to calculate the cutoff values for the LNR and PLNC that discriminate survival. The overall survival and cancer-specific survival rates were calculated. Relationships between clinicopathological factors and patient survival were assessed using univariate and multivariate analyses. RESULTS: The optimal cutoff values for the LNR and PLNC were 0.45 and 3, respectively. Univariate analysis revealed that tumor size, the American Joint Committee on Cancer stage, T stage, the LNR and PLNC were significantly associated with prognosis (P < 0.05). Multivariate analysis demonstrated that the LNR, T stage, and tumor size were independent prognostic factors for cancer-specific and overall survival, whereas PLNC was not. In the subgroup of patients with positive lymph nodes, patients with an LNR of greater than 0.45 had significantly worse cancer-specific survival (hazard ratio, 2.418; 95% confidence interval, 1.588 to 3.682; P < 0.001) and overall survival (hazard ratio, 2.149; 95% CI, 1.421 to 3.249; P < 0.001) than those with an LNR of 0.45 or less. CONCLUSIONS: The LNR was a better predictor of long-term prognosis than PLNC in patients with distal cholangiocarcinoma.

[Prevalence and epidemic characteristics of metabolic syndrome among adult residents in Nanjing City in 2010-2013].

OBJECTIVE: To understand the prevalence and epidemic characteristics of metabolic syndrome(MS) among adult residents in Nanjing City. METHODS: In 2010-2013, a total of 4918 adult residents in Nanjing City were surveyed using a multi-stage stratification and population proportional clustering random sampling method. Questionnaires, physical examinations and laboratory examinations were conducted to collect the basic conditions, health history of the subjects and related indicators including height, weight, waist circumference, blood pressure, fasting blood glucose, total cholesterol, triglycerides, high-density lipoprotein cholesterol, etc. The 2009 International Joint Interim Statement(JIS) was used to filter patients with metabolic syndrome. RESULTS: Among the 4918 surveyed subjects, 1627 were MS, with a crude prevalence rate of 33. 1% and an age-standardized prevalence rate of 26. 3%, the standardized prevalence rate of MS was 29. 0% in males and 24. 8% in females. Age-standardized prevalence rates between different genders had no significant statistical difference(χ~2=1. 352, P=0. 245). There were significant statistical differences between the crude prevalence rate of MS among subjects of different ages(χ~2=132. 557, P<0. 001), occupations(χ~2=87. 572, P<0. 001)and educational level(χ~2=26. 077, P<0. 001). Patients with a family history of hypertension(χ~2=26. 582, P<0. 001) and a family history of diabetes(χ~2=16. 013, P<0. 001)had a higher prevalence of MS. CONCLUSION: The prevalence of metabolic syndrome in adult residents in Nanjing City in 2010-2013 was relatively high.

Induction of innate immune response following introduction of infectious bronchitis virus (IBV) in the trachea and renal tissues of chickens.

Infectious bronchitis (IB) is a highly contagious respiratory disease of chickens, which is caused by the infectious bronchitis virus (IBV). The innate immune response is crucial for antiviral infections and revealing the pathogenic mechanisms of IBV. In this study, we presents an evaluation of interferon (I, II and III IFNs) in renal and tracheal samples from chickens experimentally infected previously vaccinated or not. The results suggest differential expression of chicken interferon, among them type I IFN elaborate a major role in fighting off virus. And vaccine confers greater induction ability of innate immunity thereby vaccination prior infection occurs might be necessary. Above all, we found that IFN-λ also have an effect on IBV infection in trachea besides many other respiratory virus. This study provides the first comprehensive analysis of host-virus interactions of IBV with chicken innate immune response mediated by interferon in various groups.

Association of dietary sodium:potassium ratio with the metabolic syndrome in Chinese adults.

Several epidemiological studies have investigated that Na or K intakes might be associated with the metabolic syndrome (MetS). However, little evidence has evaluated the association between Na:K ratio and the MetS. In this study, we assessed the association between the dietary Na:K ratio and the MetS. The cross-sectional study was conducted among adults aged 18 years and older in Nanjing, using a multi-stage random sampling method, which resulted in a sample size of 1993 participants. Dietary Na and K intakes were assessed by 3 consecutive days of dietary recollection combined with condiments weighing method. Health-related data were obtained by standardised questionnaires, as well as physical examinations and laboratory assessments. The prevalence rate of the MetS was 36·5 % (728/1993). After adjusting for various lifestyle and dietary factors of the MetS, participants in the highest quartile of dietary Na:K ratio were at a higher risk of developing MetS (OR=1·602; 95 % CI 1·090, 2·353) compared with those in the lowest quartile. Each 1-sd increase in dietary Na:K ratio was associated with a higher risk of prevalent MetS (OR=1·166; 95 % CI: 1·018, 1·336). Among the components of the MetS, dietary Na:K ratio was positively associated with high blood pressure (quartile 3 v. quartile 1: OR=1·656; 95 % CI 1·228, 2·256) and hypertriacylglycerolaemia (quartile 4 v. quartile1: OR=1·305; 95 % CI 1·029, 1·655) in multivariate analysis. These results revealed that higher dietary Na:K ratio significantly increased the risk of the MetS in Chinese adults. Further studies are needed to verify this association.

[Consumption and constitutional parameters of indefinite-configuration foods among urban residents in Shandong and Jiangsu Provinces].

OBJECTIVE: To explore consumption situation and composition parameter distribution of indefinite-configuration foods in diets of some urban adult Chinese residents, and establish the parameters databases. METHODS: A total of 1620 adult urban residents were sampled randomly by a multi-stage sampling method from four cities in Jiangsu and Shandong Provinces, and were investigated with a self-made Food Frequency Questionnaire. The moisture content status of indefinite-configuration foods from the market were analysed with the method of China national standards GB 5009. 3-2010, then raw material composition of indefinite-configuration foods was calculated according to their moisture content. Kinds of food samples were simulated and took visual foods pictures in lab. RESULTS: Consumption rate of indefinite-configuration foods was higher in the last month. When eating outside, consumption rate of buns and soya-bean milk foods in whole diet were as high as 55. 6% and 40. 4%. When eating at home, consumptionratio of porridge and soup was more than 50%. Daily intake of rice porridge, soya-bean milk reached as high as 30. 2% and 22. 2%. 100 g samples of the rice gruel contained rice 9. 2 g, 100 g soya-bean milk contained soybean 8. 2 g, 100 g pork buns contained wheat flour 42. 3 g and minced cooked meat 20. 7 g by laboratory analysis. CONCLUSION: Indefinite-configuration foods make up a large proportion of the whole diet, so it 's necessary to research the stationary raw material component parameters of the food and establish the parameters database for the dietary evaluator and nutrition instructor.

HSPB8 Promotes the Fusion of Autophagosome and Lysosome during Autophagy in Diabetic Neurons.

Although autophagy has been proposed to play an emerging role in diabetic neuropathy, autophagy and its possible role remains unclear. Moreover, only few studies about diabetes have explored the autophagy mediated by heat shock protein beta-8 (HSPB8) and Bcl-2 associated athanogene 3 (BAG3). In the present study, we examined the autophagy induced by high glucose levels in an in vivo rat model of diabetes induced by streptozotocin (STZ) and an in vitro model of retinal ganglion cell-5 (RGC5) cells under high glucose conditions. In the spinal cord tissues of the STZ-induced diabetic rats, the levels of light chain 3 (LC3) and Beclin-1-marked autophagy rose with increasing HSPB8 and BAG3 levels. By confocal immunofluorescence, HSPB8 and LC3 were observed to be co-localized in the spinal cord tissues. In the RGC5 cells, high-glucose stimulation upregulated the expression of LC3-Ⅱ, Beclin-1, and HSPB8 in a dose-dependent manner. When the RGC5 cells were subjected to high-glucose conditions, HSPB8 overexpression, along with upregulated LC3-Ⅱ and Beclin-1 expression, increased the autophagic rate, whereas siRNA-silenced HSPB8 decreased the autophagic rate. Furthermore, in GFP-mRFP-LC3 probe experiments, HSPB8 overexpression promoted autophagosome-lysosome fusion, whereas HSPB8 silencing disrupted this process. In the cells treated with HSPB8 and siRNA, the fusion was impaired, as indicated by the elevated p62 expression. HSPB8 overexpression can partly rescue the blocking of the autophagy flux with chloroquine through the reduction of p62 expression level. Our study demonstrated that HSPB8 is involved in the high glucose-induced autophagy under the in vivo and in vitro conditions and critically participated in the autophagosome-lysosome fusion during the autophagy flux.

[Analysis of dietary iron intakes of pregnant and lactating women based on food composition databases from China and US].

OBJECTIVE: To evaluate the difference between estimations on dietary iron intake based on the China and US food composition databases. METHODS: Total 368 records of 24-h dietary recall on mid-term pregnant women and lactating rural women were analyzed for their iron intakes with the China Food Composition Table 2002 and the USDA National Nutrient Database for Standard Reference release 25, respectively. The values of dietary iron intake derived from two composition databases were compared statistically. RESULTS: The dietary iron intakes of total 368 dietary records estimated with China and US databases were (24.37 +/- 9.66) mg and (16.20 +/- 9.13) mg respectively (with paired t test, t = 20.081, P < 0.01, correlation coefficient r = 0.657, P < 0.01), with average ratio of 1.69 +/- 0.55 between China and US values. In terms of food classification, the most significant differences were with dairy products, fishes, fruits, meats and cereals, with ratios of (10.66 +/- 2.24), (5.10 +/- 5.51), (3.01 +/- 2.26), (3.01 +/- 7.85) and (2.33 +/- 0.77), respectively. Only iron intake values from tuberous crops and soy products had inverse ratio as (0.83 +/- 0.50) and (0.75 +/- 0.53). CONCLUSION: The averaged dietary iron intake value for total records was very close to the reported national levels when estimated with the China Food Composition Table 2002, but much decreased when estimated with US food composition database with values as only approximately 66% of reported levels.

Excellent mechanical and electromagnetic interference shielding properties of polylactic acid/polycaprolactone/multiwalled carbon nanotube composites enabled by a multilayer structure design.

In this work, a special multilayer structure consisting of polylactic acid (PLA) and a co-continuous PLA/polycaprolactone (PCL)/multiwalled carbon nanotube (MWCNT) (ALM) composite with a double-percolated conductive network was fabricated via layer-assembly coextrusion. It was revealed that PLA domains located at the layer interface could serve as rivets properly linking adjacent layers. Such a nacre-like structure with alternately stacked rigid PLA and flexible ALM increased the fracture strain to 354.4%, nearly quadruple that of the PLA/PCL/MWCNT conventional blending composite with the same composition, while maintaining an excellent strength above 46.0 MPa. In addition, the multilayer composites showed a special frequency-selective electromagnetic interference (EMI) shielding performance, with tunable shielding peak positions controlled by the layer number. Their maximum EMI shielding effectiveness almost contributed by absorption loss could reach 49.8 dB, which originated from two aspects: one was the high electrical conductivity offered by the double-percolated distribution of MWCNTs, and the other was the multiple wave attenuation effect that occurred at the interfaces between PLA and ALM layers and the blend interfaces in ALM layers. This effort paves a new way for developing composites with outstanding mechanical and EMI shielding properties that can be extended to other polymeric composite systems.