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Inflammatory response and oxidative stress are considered to be important mechanisms of lung injury induced by lunar dust. However, the pulmonary toxicological mechanism remains unclear. In the present study, Wistar rats were exposed to CLDS-i 7 days/week, 4 h/day, for 4 weeks in the mouth and nose. Lung tissue samples were collected for histopathological analysis and ultra-performance liquid chromatography-mass spectrometry analysis. Enzyme activities and expression levels of key metabolic enzymes were detected by biochemical analysis and real-time PCR. The pathological features of lung tissue showed that CLDS-i caused congestion and inflammation in the lungs, and the lung structure was severely damaged. Metabolomics analysis showed that 141 metabolites were significantly changed in the lung tissue of the CLDS-i group compared with the control group. Combined with Kegg pathway analysis, it was found that the changes of amino acid metabolites were involved in these pathways, indicating that the simulated lunar dust exposure had the most obvious effect on amino acid metabolism in the lung tissue of rats. Real-time PCR analysis showed that the mRNA expression of six key enzymes related to amino acid metabolism was changed, and the enzyme activities of these key enzymes were also changed, which were consistent with the results of qPCR. These results suggest that changes in amino acid metabolism may be closely related to the pathogenesis of lung injury induced by lunar dust, and amino acid metabolism may be a potential biomarker of lung diseases related to lunar dust exposure.
Assuntos
Pneumopatias , Lesão Pulmonar , Ratos , Animais , Poeira/análise , Lesão Pulmonar/metabolismo , Ratos Wistar , Pulmão , Pneumopatias/metabolismo , Metabolômica , Aminoácidos/toxicidade , Aminoácidos/metabolismoRESUMO
Lunar dust is one of the biggest risk factors in the future manned exploration mission. Much is not known about the pulmonary toxicity of lunar dust. The aim of this study was to evaluate the lung inflammation and oxidative stress induced by subacute exposure to lunar dust stimulant (LDS) in rats. Wistar rats were intratracheally administered LDS, twice a week for 3 weeks. Inflammatory cell counting and cytokine assays using bronchoalveolar lavage fluid (BALF) were performed. Lung tissues were processed for histopathological examination and immunohistochemical staining. Biomarkers of oxidative stress and genes and proteins related to inflammation and fibrosis in lung tissue were also determined. The neutrophil count in the BALF of LDS-exposed groups was higher than that in controls (P < .05). LDS caused a significant increase in some of biochemical indicators and proinflammatory factors levels in BALF compared with control group. The normal balance between oxidation and antioxidation was broken by LDS. Pathological characteristics of lung tissue and immunohistochemical results for α-smooth muscle actin (α-SMA) indicated that inflammatory response was an extremely important passage to pulmonary fibrosis. Real-time PCR analysis showed elevated levels of nitric oxide synthase (NOS) and nicotinamide adenine dinucleotide phosphate oxidase (NOX) mRNA in the lungs (P < .05). Western blotting results were consistent with immunohistochemistry and qPCR results. These results indicate that inhalation of lunar dust may cause inflammatory pulmonary fibrosis. NOX4 may be a key potential therapeutic target for inflammatory injury and fibrosis in the lung.
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Poluentes Atmosféricos/toxicidade , Poeira Cósmica/efeitos adversos , Poeira/análise , Pulmão/efeitos dos fármacos , Pneumonia/induzido quimicamente , Fibrose Pulmonar/induzido quimicamente , Poluentes Atmosféricos/química , Animais , Líquido da Lavagem Broncoalveolar/citologia , Líquido da Lavagem Broncoalveolar/imunologia , Contagem de Células , Citocinas/imunologia , Exposição por Inalação , Pulmão/metabolismo , Pulmão/patologia , Masculino , Tamanho da Partícula , Pneumonia/metabolismo , Pneumonia/patologia , Fibrose Pulmonar/metabolismo , Fibrose Pulmonar/patologia , Ratos , Ratos WistarRESUMO
Lunar dust particles are an environmental threat to lunar astronauts, and inhalation of lunar dust can cause lung damage. The current study explored the mechanism of lunar dust simulant (CLDS-i) inducing inflammatory pulmonary injury. Wistar rats were exposed to CLDS-i for 4 h/d and 7d/week for 4 weeks. Pathological results showed that a large number of inflammatory cells gathered and infiltrated in the lung tissues of the simulated lunar dust group, and the alveolar structures were destroyed. Transcriptome analysis confirmed that CLDS-i was mainly involved in the regulation of activation and differentiation of immune inflammatory cells, activated signaling pathways related to inflammatory diseases, and promoted the occurrence and development of inflammatory injury in the lung. Combined with metabolomics analysis, the results of joint analysis of omics were found that the genes Kmo, Kynu, Nos3, Arg1 and Adh7 were involved in the regulation of amino acid metabolism in rat lung tissues, and these genes might be the key targets for the treatment of amino acid metabolic diseases. In addition, the imbalance of amino acid metabolism might be related to the activation of nuclear factor kappaB (NF-κB) signaling pathway. The results of quantitative real-time polymerase chain reaction and Western blot further confirmed that CLDS-i may promote the occurrence and development of lung inflammation and lead to abnormal amino acid metabolism by activating the B cell activation factor (BAFF)/ B cell activation factor receptor (BAFFR)-mediated NF-κB signaling pathway.
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The existence of molecular H2O and evolution of solar wind-derived water on the lunar surface remain controversial. We report that large amounts of OH and molecular H2O related to solar wind and other multiple sources are preserved in impact glasses from Chang'e-5 (CE5) lunar soil based on reflectance infrared spectroscopy and nanoscale secondary ion mass spectrometry analyses. The estimated water content contributed by impact glasses to CE5 lunar soil was ~72 ppm, including molecular H2O of up to 15 to 25 ppm. Our studies revealed that impact glasses are the main carrier of molecular H2O in lunar soils. Moreover, water in CE5 impact glasses provides a record of complex formation processes and multiple water sources, including water derived from solar wind, deposited by water-bearing meteorites/micrometeorites, and inherited from lunar indigenous water. Our study provides a better understanding of the evolution of surficial water on airless bodies and identifies potential source and storage pathways for water in the terrestrial planets.
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Lunar exploration is deemed crucial for uncovering the origins of the Earth-Moon system and is the first step for advancing humanity's exploration of deep space. Over the past decade, the Chinese Lunar Exploration Program (CLEP), also known as the Chang'e (CE) Project, has achieved remarkable milestones. It has successfully developed and demonstrated the engineering capability required to reach and return from the lunar surface. Notably, the CE Project has made historic firsts with the landing and on-site exploration of the far side of the Moon, along with the collection of the youngest volcanic samples from the Procellarum KREEP Terrane. These achievements have significantly enhanced our understanding of lunar evolution. Building on this success, China has proposed an ambitious crewed lunar exploration strategy, aiming to return to the Moon for scientific exploration and utilization. This plan encompasses two primary phases: the first crewed lunar landing and exploration, followed by a thousand-kilometer scale scientific expedition to construct a geological cross-section across the lunar surface. Recognizing the limitations of current lunar exploration efforts and China's engineering and technical capabilities, this paper explores the benefits of crewed lunar exploration while leveraging synergies with robotic exploration. The study refines fundamental lunar scientific questions that could lead to significant breakthroughs, considering the respective engineering and technological requirements. This research lays a crucial foundation for defining the objectives of future lunar exploration, emphasizing the importance of crewed missions and offering insights into potential advancements in lunar science.
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Self-polymerization epoxy/phthalonitrile (APPEN) pre-polymers were studied systematically, and then, gelation time and differential scanning calorimetry (DSC) were employed to investigate their curing behaviors. Taking advantage of orthogonal test analysis, the key factors that affected the co-polymerization of APPEN were defined and the appropriate pre-polymerization conditions were analyzed. A possible curing mechanism of APPEN was proposed. Then, the thermomechanical and mechanical properties of glass-fiber-reinforced APPEN laminates (APPEN/GF) prepared at 180 °C were analyzed to understand the cross-linked and aggregation structures. Fracture surface of the composite laminates was also investigated to reveal the copolymerization degree and the interfacial binding. The results indicated that APPEN/GF composites exhibit outstanding mechanical and thermomechanical properties (flexural strength, 712 MPa, flexural modulus, 38 GPa, and Tg > 185 °C). The thermal stability (T5% > 334 °C and IPDT reached 1482 °C) of APPEN/GF composites was also investigated to further reveal the copolymerization between epoxy resin and aminophthalonitrile, which may be beneficial to the application of epoxy-matrix-based composites in the field of high-performance polymer composites.
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Frequent impacts on the Moon have changed the physical and chemical properties of the lunar regolith, with new materials deposited from the impact-induced vapor phase. Here, we combined nanoscale chemical and structural analysis to identify the mineral digenite (4Cu2S·CuS) in Chang'e-5 lunar soil. This is the first report of digenite in a lunar sample. The surface-correlated digenite phase is undifferentiated in distribution and compositionally distinct from its hosts, suggesting that it originated from vapor-phase deposition. The presence of an Al-rich impact glass bead suggests that a thermal effect provided by impact ejecta is the main heat source for the evaporation of Cu-S components from a cupriferous troilite precursor, and the digenite condensed from these Cu-S vapors. A large pure metallic iron (Fe0) particle and high Cu content within the studied Cu-Fe-S grain suggest that this grain was most likely derived from a highly differentiated and reduced melt.
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The Zhurong rover of the Tianwen-1 mission landed in southern Utopia Planitia, providing a unique window into the evolutionary history of the Martian lowlands. During its first 110 sols, Zhurong investigated and categorized surface targets into igneous rocks, lithified duricrusts, cemented duricrusts, soils and sands. The lithified duricrusts, analysed by using laser-induced breakdown spectroscopy onboard Zhurong, show elevated water contents and distinct compositions from those of igneous rocks. The cemented duricrusts are likely formed via water vapor-frost cycling at the atmosphere-soil interface, as supported by the local meteorological conditions. Soils and sands contain elevated magnesium and water, attributed to both hydrated magnesium salts and adsorbed water. The compositional and meteorological evidence indicates potential Amazonian brine activities and present-day water vapor cycling at the soil-atmosphere interface. Searching for further clues to water-related activities and determining the water source by Zhurong are critical to constrain the volatile evolution history at the landing site.
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Irradiation structural damage (e.g., radiation tracks, amorphous layers, and vesicles) is widely observed in lunar soil grains. Previous experiments have revealed that irradiation damage is caused by the injection of solar wind and solar flare energetic particles. In this study, cordierite and gabbro were selected as analogs of shallow and deep excavated lunar crust materials for proton irradiation experiments. The fluence was 1.44 ± 0.03 × 1018 H+/cm2, which is equivalent to 102 years of average solar wind proton implantation on the Moon. Before and after irradiation, structural damage in samples is detected by slow positron annihilation technology (PAT), Doppler broadening (DB) measurement, focused ion beam (FIB), and transmission electron microscopy (TEM). The DB results showed the structural damage peaks of irradiated gabbro and cordierite were located at 40 and 45 nm. Hydrogen diffused to a deeper region and it reached beyond depths of 150 and 136 nm for gabbro and cordierite, respectively. Hydrogen atoms occupied the original vacancy defects and formed vacancy sites-hydrogen atom complexes, which affected the annihilation of positrons with electrons in the vacancy defects. All of the DB results were validated by TEM. This study proves that the positron annihilation technique has an excellent performance in the detection of defects in the whole structure of the sample. In combination with TEM and other detection methods, this technology could be used for the detection of structural damage in extraterrestrial samples.
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The formation and distribution of lunar surficial water remains ambiguous. Here, we show the prominence of water (OH/H2O) attributed to solar wind implantation on the uppermost surface of olivine, plagioclase, and pyroxene grains from Chang'E-5 samples. The results of spectral and microstructural analyses indicate that solar wind-derived water is affected by exposure time, crystal structure, and mineral composition. Our estimate of a minimum of 170 ppm water content in lunar soils in the Chang'E-5 region is consistent with that reported by the Moon Minerology Mapper and Chang'E-5 lander. By comparing with remote sensing data and through lunar soil maturity analysis, the amount of water in Chang'E-5 provides a reference for the distribution of surficial water in middle latitude of the Moon. We conclude that minerals in lunar soils are important reservoirs of water, and formation and retention of water originating from solar wind occurs on airless bodies.
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Reducing lunar dust adhesion to various material surfaces is important for protecting equipment from damage during lunar exploration missions. In this study, we investigate the lunar dust-mitigation ability and dust adhesion force of aluminum (Al) substrates prepared using different etching methods. Among them, composite etching methods (combining chemical and electrochemical steps) can result in multiscale structures with micro- and nanoroughness, reducing the contact area between the substrate and thus decreasing lunar dust adhesion. After composite etching, the dust adhesion force of the Al substrate was significantly reduced by 80% from 45.53 to 8.89 nN. The dust adhesion force of Al substrates dominates their dust-mitigation performance in floating dust environments. The lunar dust coverage (2.19%) of the Al substrate modified by composite etching (placed with a tilt angle of 90°) was 4-fold lower than that of the pristine Al substrate (9.11%), indicating excellent lunar-dust repellence. In addition, other factors such as tilt angle of the substrate and dust loading significantly affect dust-mitigation performance of the modified Al substrates. The Al substrate with an excellent dust-mitigation ability highlights good potential for lunar exploration missions.
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Ferric iron as well as magnetite are rarely found in lunar samples, and their distribution and formation mechanisms on the Moon have not been well studied. Here, we discover sub-microscopic magnetite particles in Chang'E-5 lunar soil. Magnetite and pure metallic iron particles are embedded in oxygen-dissolved iron-sulfide grains from the Chang'E-5 samples. This mineral assemblage indicates a FeO eutectoid reaction (4FeO = Fe3O4 + Fe) for formation of magnetite. The iron-sulfide grains' morphology features and the oxygen's distribution suggest that a gas-melt phase reaction occurred during large-impact events. This could provide an effective method to form ubiquitous sub-microscopic magnetite in fine lunar soils and be a contributor to the presentation of ferric iron on the surface of the Moon. Additionally, the formation of sub-microscopic magnetite and metallic iron by eutectoid reaction may provide an alternative way for the formation of magnetic anomalies observed on the Moon.