BPA promotes lung fibrosis in mice by regulating autophagy-dependent ferroptosis in alveolar epithelial cells.

BACKGROUND: Bisphenol A (BPA) is an industrial chemical that is commonly found in daily consumer products. BPA is reportedly associated with lung diseases. However, the impact of BPA on pulmonary fibrosis (PF) and its possible mechanisms of action both remain unclear. METHODS: A PF mouse model was induced by bleomycin (BLM). Mouse lung fibroblasts (MLG 2908) and mouse alveolar epithelial cells (MLE-12) were treated with BPA to establish a PF cell model. Tissue staining, CCK-8 assays, western blot experiments and relevant indicator kits were used to detect and evaluate the effect of BPA on PF. RESULTS: BPA dose-dependently promoted oxidative stress and induced ferroptosis, leading to PF. The ferroptosis inhibitor Fer-1 partly attenuated the effect of BPA. In addition, among the two main cell types associated with the progression of PF, MLE-12 cells are more sensitive to BPA than are MLG 2908 cells, and BPA induces ferroptosis in MLE-12 cells. Furthermore, BPA promoted autophagy-mediated ferroptosis by activating the AMPK/mTOR signaling pathway, thereby exacerbating the progression of PF. The autophagy inhibitor CQ1 partly attenuated the effect of BPA. CONCLUSION: BPA promotes the progression of PF by promoting autophagy-dependent ferroptosis in alveolar epithelial cells, which provides a new theoretical basis for understanding BPA-induced PF.

Biochar modification to enhance arsenic removal from water: a review.

Arsenic (As) contamination is a major threat to drinking water quality throughout the world, and the development of appropriate remediation methods is critical. Adsorption is considered the most effective method for remediation of As-contaminated water. Biochar is a promising adsorbent and widely discussed for As removal due to its potential low cost and environmental friendliness. However, pristine biochar generally exhibited relatively low adsorption capacity for As mainly due to the electrostatic repulsion between the negatively charged biochar and As. Biochar modification, especially metal modification, was developed to boost the adsorption capacity for As. A systematic analysis of As removal as affected by biochar properties and modification will be of great help for As removal. This paper presents a comprehensive review on As removal by biochars from different feedstock, preparation procedures, and modification methods, with a major focus on the possible mechanisms of interaction between As and biochar. Biochar derived from sewage sludge exhibited relatively high adsorption capacity for As. Considering energy conservation, biochars prepared at 401-500 °C were more favorable in adsorbing As. Fe-modified biochar was the most popular modified biochar for As remediation due to its low cost and high efficiency. In addition, the limitations of the current studies and future perspectives are presented. The aim of this review is to provide guidance for the preparation of low-cost, environmentally friendly, and high efficiency biochar for the remediation of As-contaminated water.

Characterization of the ryanodine receptor gene in Encarsia formosa (Gahan) and its expression profile in response to diamide insecticides.

Ryanodine receptors (RyRs) are the targets of diamide insecticides, which have been identified and characterized in a dozen insect pests of Lepidoptera, Hemiptera, Diptera and Coleoptera, but limited attention has been paid to the RyR in parasitoid natural enemies. Without this knowledge, it will hinder our effective and efficient application using both parasitoid natural enemies and diamide insecticides simultaneously in the integrated pest management (IPM). In this study, the full-length cDNA of RyR was cloned from Encarsia formosa (EfRyR), a parasitic wasp used worldwide for the biological control of whitefly. Its expression profile was examined in various tissues of E. formosa adults. The toxicities of four diamide insecticides to E. formosa were measured, and then the expression profile of EfRyR after 12 h and 24 h exposure to the LC50 dosages of diamide insecticides was investigated. The results showed that the full-length cDNA of EfRyR was 16, 778 bp including a 15, 345 bp open reading frame, and two alternative splice (AS) sites. Comparing to its expression in the abdomen, EfRyR was highly expressed in the head (11.9-fold) and the thorax (3.7-fold). The toxicities of four dimide insecticides against E. formosa from low to high were chlorantraniliprole (LC50 = 367.84 mg L-1), cyantraniliprole (221.72 mg L-1), cyclaniliprole (51.77 mg L-1), and tetrachlorantraniliprole (8.35 mg L-1). The expressions of EfRyR and its variants with AS were significantly increased after E. formosa adults were exposed to different diamide insecticides. This study improves our understanding of the RyR in parasitoid wasps and provides useful information on IPM by using E. formosa.

Negative Impacts of Biochars on Urease Activity: High pH, Heavy Metals, Polycyclic Aromatic Hydrocarbons, or Free Radicals?

Although biochars show promise for soil remediation, their negative impacts have not been systematically assessed. This study investigated the effects of corn cob biochars on urease-mediated urea hydrolysis and attempted to determine the mechanisms underlying those effects. The results showed that biochars inhibited urease activity (indicated by the NH4+ generation). Released heavy metals and polyaromatic hydrocarbons explained the 20% inhibition of urease activity in biochar supernatants (200 °C) but could not explain the 70% inhibition in particle systems (400 °C). The levels of protein carbonyl and nitrotyrosine, common oxidative damagers of urease, were high in particle systems but very low in biochar supernatants. The electron paramagnetic resonance signal intensity reached its highest level in the 400 °C biochar and decreased in the 500 °C biochar, possibly due to the decomposition of organic molecules. The observed inhibition of urease activity may be the result of oxidative reactions with free radicals on the biochar surface or oxidative reactions with reactive oxygen species promoted by free radicals. We suggest that these potential hazards be evaluated further to gauge the relevance of these findings to field conditions and to assist in the development of safe and sustainable application schemes for biochars.

Overexpression of NLRP3, NLRC4 and AIM2 inflammasomes and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in Malassezia folliculitis.

The activation of NLRP3, NLRC4 and AIM2 inflammasomes is pivotal for innate immunity against some pathogenic fungi, but their role in the pathogenesis of Malassezia folliculitis (MF) remains unclear. The objective of the study was to determine the expression of 4 canonical inflammasomes (NLRP1, NLRP3, NLRC4 and AIM2) and their priming-associated molecules (TLR2, TLR4, Dectin-1, Dectin-2 and NFκB) in MF lesion. Expression of NLRP1, NLRP3, NLRC4, AIM2, caspase-1, IL-1ß, TLR2, TLR4, Dectin-1, Dectin-2 and NFκB was detected by immunohistochemistry in skin lesion of 23 MF patients and normal skin of 12 healthy subjects. Furthermore, NLRP1, NLRP3, NLRC4, AIM2, caspase-1 and IL-1ß mRNA was measured by quantitative real-time PCR (qRT-PCR) in 12 MF cases and 10 controls. Immunohistochemical analysis revealed that NLRP3, NLRC4, AIM2, Casp-1, IL-1ß, TLR2, TLR4, Dectin-1, Dectin-2 and NFκB expression was up-regulated in the epidermis and dermal inflammatory cells of MF lesion compared with control skin (P < .01-.05), but NLRP1 expression was not different between both groups (P > .05). qRT-PCR showed that levels of NLRP3, Casp-1 and IL-1ß mRNA were significantly increased (P < .01-.05), whereas those of NLRP1, NLRC4 and AIM2 mRNA were slightly augmented compared to control skin (P > .05). Our observation suggests that simultaneous activation of NLRP3, NLRC4 and AIM2 inflammasomes may play an important role in the pathogenesis of MF.

[The new applications of NMR technology in the field of characterization of surface properties of the material].

Nuclear magnetic resonance (NMR) technology has been developed continuely because of its rapid, accurate and high resolution advantages, The technology has become an important method to analyze the soil properties, and to identify the structure of matters, food analysis, medical imaging fields. This study summarized two aspects of the important applications of NMR in the characterization of the surface properties of materials. First, the use of NMR techniques for the adsorption, desorption and diffusion behaviors of polymer on solid particles (mainly SiO2 particles). Sencond, to investigate the wettability and water uptake progress of contaminated soils using NMR techniques. This study summarized the important applications of NMR techniques in the characterization of surface properties. It also showed the unique application in the field of polymer materials and environment. This study introduced the measured method for the relaxation time of substance using acorn surface area analyzer in the liquid environment. It reflect the surface properties and structural features of substance. It also provided data support for the explanation of environmental behaviors of contaminants and thus, it played an important role in the environmental field. Compared to the conventional BET method, acorn surface area analyzer showed the advantages in terms of conditions and time measurement. Especially, traditional BET method cannot get the information of the specific surface area of substances in the liquid directly, but acorn surface area analyzer solved the difficult problem. It is a new breakthrough in the field of characterization of the surface properties of materials by NMR in the liquid. We analyzed the application of acorn surface area analyzer in pharmaceuticals, cosmetic materials, electronic products because acorn surface area analyzer could reflects the important characteristics of the surface properties and structural features of substance. And we provide the prospective of the applications of NMR techniques in organic matter of soils, drug and natural products detecting.

Platinum-catalyzed anti-stereocontrolled ring-opening of oxabicyclic alkenes with Grignard reagents.

A new platinum-catalyzed anti-stereocontrolled ring-opening of oxabicyclic alkenes with various Grignard reagents was reported, which afforded the corresponding anti-2-substituted-1,2-dihydronaphthalen-1-ol products with moderate to good yields in the presence of a catalytic amount of Pt(PPh3)4 (2.5 mol%) under mild conditions. The effects of catalyst loading, solvent and temperature on the yield were also investigated. Furthermore, the trans-configuration of the product 5i was confirmed by X-ray diffraction analysis.

Role of miR-276-3p in the cyantraniliprole resistance mechanism of Bemisia tabaci via CYP6CX3 targeting.

The sweet potato whitefly, Bemisia tabaci, is an important insect pest that transmits over 200 different plant viruses and causes serious damage to the production of cotton and Solanaceae vegetables. Cyantraniliprole is the first diamide insecticide, showing toxicity against B. tabaci. However, B. tabaci has developed resistance to this insecticide by upregulating the expressions of cytochrome P450 genes such as CYP6CX3, while there is limited information on the regulatory mechanism mediated by miRNA. In the present study, ten miRNAs were predicted to target CYP6CX3, in which miR-276-3p showed an inverse expression pattern with CYP6CX3 in two cyantraniliprole resistant strains and under cyantraniliprole exposure. A luciferase assay demonstrated that miR-276-3p suppressed CYP6CX3 expression by pairing with residues 1445-1453. Overexpression or knockdown of miR-276-3p directly impacted B. tabaci resistance to cyantraniliprole. In addition, exposure to cyantraniliprole led to a significant reduction in the expressions of five genes (drosha, dicer1, dicer2, Ago1, and Ago2A) associated with miRNA biogenesis. Suppressing genes such as drosha, dicer1, and Ago2A reduced the expression of miR-276-3p, increased CYP6CX3 expression, and decreased B. tabaci resistance to cyantraniliprole. These results improve our understanding of the role of miRNAs in P450 regulation and cyantraniliprole resistance in B. tabaci.

Expression profile of CYP402C1 and its role in resistance to imidacloprid in the whitefly, Bemisia tabaci.

Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cosmopolitan insect pest causing serious damage to crop production. Cytochromes P450 (CYPs) of B. tabaci are widely known to be involved in the metabolic resistance to a variety of insecticides, continuously increasing the difficulty in controlling this pest. In this study, four P450 genes (CYP6CM1, CYP6CX1, CYP6CX3, and CYP402C1) in B. tabaci exhibited correlations with the resistance to imidacloprid. We have focused on trying to understand the function and metabolism capacity of CYP402C1. The expression profiles of CYP402C1 were examined by reverse transcription quantitative real-time PCR and fluorescence in situ hybridizations. Its role in resistance to imidacloprid was investigated by RNA interference, transgenic Drosophila melanogaster, and heterologous expression. The results showed that CYP402C1 was highly expressed in the active feeding stages of B. tabaci, such as nymphs and female adults. CYP402C1 was mainly expressed in midguts of nymphs and adults, especially in the filter chamber. Knockdown of CYP402C1 significantly decreased the resistance of B. tabaci to imidacloprid by 3.96-fold (50% lethal concentration: 186.46 versus 47.08 mg/L). Overexpression of CYP402C1 in a transgenic D. melanogaster line (Gal4 > UAS-CYP402C1) significantly increased the resistance to imidacloprid from 12.68- to 14.92-fold (129.01 and 151.80 mg/L versus 1925.14 mg/L). The heterologous expression of CYP402C1 showed a metabolism ability of imidacloprid (imidacloprid decreased by 12.51% within 2 h). This study provides new insights for CYP402C1 function in B. tabaci and will help develop new strategies in B. tabaci control and its insecticide resistance.

Sorption of organic contaminants by biochars with multiple porous structures: Experiments and molecular dynamics simulations mediated by three-dimensional models.

Interactions between organic pollutants and carbon-based particles are critical for understanding and predicting the fate of organic contaminants in the environment. However, traditional modeling concepts did not consider three-dimensional (3-D) structures of carbon-based materials. This prevents a deep understanding of the sequestration of organic pollutants. Therefore, this study revealed interactions between organics and biochars by combining experimental measurements and molecular dynamics simulations. Biochars displayed the best and worst sorption performances for naphthalene (NAP) and benzoic acid (BA), respectively, among the five adsorbates. The kinetic model fitting suggested that biochar pores played a vital role during sorption and led to the fast and slow sorption of organics on the biochar surface and in pores, respectively. Active sites on the biochar surface predominantly sorbed organics. Organics were only sorbed in pores when the surface's active sites were fully occupied. These results can guide the development of efficient organic pollution control strategies to protect human health and improve ecological security.

The opposite influences of Cu and Cd cation bridges on sulfamethoxazole sorption on humic acids in wetting-drying cycles.

Wetting-drying cycles in the environment could change the inner- or outer-sphere complexation of heavy metal cations on natural organic matter (NOM) and then influence ternary interactions with organic contaminants - a rarely-discussed essential geochemical process. In this work, the sorption of sulfamethoxazole (SMX) on humic acids (HAs) mediated by cations (Cu2+ and Cd2+) was investigated. Considering that outer-sphere complexation could be transformed into inner-sphere complexation during vacuum freeze-drying, the role of inner- or outer-sphere complexation on SMX sorption was explored. The experimental sorption results and density functional theory (DFT) calculations suggested that Cu2+ and Cd2+ sorption on HAs was mainly outer- and inner-sphere complexation, respectively. Cd2+ consistently promoted SMX sorption on HAs, while Cu2+ promoted and inhibited SMX sorption before and after freeze-drying. The structure of HA-Cu complexes with inner-sphere complexation was more compact than those with outer-sphere complexation, which reduced the accessibility of sorption sites for SMX on HA-Cu and inhibited SMX sorption. However, the greater number of coordination sites of Cd2+ may provide more sorption sites and the structure of HA-Cd was looser. These findings provide a groundbreaking understanding of the sorption of organics on natural adsorbents in the presence of cations.

The promoted degradation of biochar-adsorbed 2,4-dichlorophenol in the presence of Fe(III).

Organic pollutant degradation by biochar could be promoted by Fe because of the Fenton-like reaction. However, studies have also confirmed that reactive oxygen species (ROS) play only a limited role in organic pollutant degradation by biochar. Herein, we quantitatively identified 2,4-dichlorophenol (2,4-DCP) adsorption and degradation in Fe-biochar systems and obtained degradation (k1) and adsorption rate constants (k2) by two-compartment first-order kinetics modeling. The k1 was approximately 7-10 times lower than the corresponding k2 and the positive correlation between k1 and k2 illustrated that adsorption and degradation were kinetically associated. ROS quenching only slightly inhibited 2,4-DCP degradation. Chemicals with similar structures to ROS quenchers (without quenching ability) also inhibited 2,4-DCP degradation, probably because of the competition of the active degradation sites on biochars. Electrochemical analysis and pH-impact experiments further elucidated that 2,4-DCP underwent oxidation-dominated degradation in the adsorbed phase via direct electron transfer. Fe(III) obviously increased 2,4-DCP adsorption through cation bridging and enhanced electron density by Fe-O conjugations on the biochar surface, which facilitated subsequent degradation. This study emphasized the importance of degradation on the biochar solid phase and that a breakthrough of the mass transfer bottleneck of adsorption will greatly promote degradation.

Association of C-reactive protein gene polymorphisms with the risk of ischemic stroke: A systematic review and meta-analysis.

BACKGROUND AND PURPOSE: The heterogeneous, complex condition known as ischemic stroke (IS) is brought on by the interaction of a number of risk factors and genetic variables. The association between C-reactive protein (CRP) gene polymorphisms and IS has, however, been the subject of inconsistent findings. Therefore, we conducted a meta-analysis to comprehensively address possible associations of CRP genes with the risk of IS. METHODS: A comprehensive literature search for all the published articles was performed in electronic databases including PubMed, EMBASE, Cochrane Library, and Google Scholar from January 1, 1950 to June 30, 2022. Odds ratio (OR) with 95% Confidence interval (CIs) along with fixed/random effect models were used to calculate summary estimates. RESULTS: Twelve case-control studies totalling 3880 IS cases and 5233 controls were included for the association of CRP gene polymorphisms (rs1800947, rs1130864, rs3093059, rs2794521, and rs1205). Across all genotyping models, we discovered that rs1130864, rs3093059, rs2794521, and rs1205SNPs were not substantially related to IS risk. A trend for significant association for rs1800947 under dominant (OR = 1.19; 95% CI = 0.97 to 1.48), recessive (OR = 1.49; 95% CI = 0.71 to 3.14) and allelic model (OR = 1.21; 95% CI = 0.99 to 1.48) was observed. However, protective association for rs1130864 under dominant (OR = 0.80; 95% CI = 0.70 to 0.91) and rs3093059 under allelic model (OR = 0.18; 95% CI = 0.14 to 0.22) was found. CONCLUSION: Our thorough study revealed that the CRP gene variants rs1800947, rs1130864, rs3093059, rs2794521, and rs1205 could not be related to the risk of ischemic stroke. However, additional research must focus on the rs1800947 polymorphisms in a particular group.

Molecular clusters played an important role in the adsorption of polycyclic aromatic hydrocarbons (PAHs) on carbonaceous materials.

Polycyclic aromatic hydrocarbons (PAHs) are one of the most frequently detected hydrophobic organic contaminants (HOCs) in the environment. They may form clusters because of the strong hydrophobic and π-π electron-donor-acceptor (EDA) interactions among PAHs molecules. However, previous experimental studies and theoretical simulations generally ignored the impact of molecular clusters on the adsorption, which may result in the misunderstanding of the environmental fate and risk. In this work, naphthalene (NAP), phenanthrene (PHE), and pyrene (PYR) were selected to investigate intermolecular interaction as well as the consequent impact on their adsorption on graphene. The density field of C atoms in equilibrium configurations of self-interacted PAHs suggested that the formation of PAHs molecular clusters was a spontaneous process, and was favored in solvents with stronger polarity and for PAHs with more benzene rings. It should be noted that the molecular dynamics simulations with the initial state of molecular clusters matched better with the published experimental results compared with those of individual PAHs. The formed compact PAHs clusters in polar solvents increased the apparent PAHs adsorption, because of their higher hydrophobic and π-π EDA interactions. This study emphasized that the self-interaction of PAHs should be carefully considered in both experimental and theoretical simulation studies.