Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
1.
Inorg Chem ; 62(3): 1086-1094, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36622819

RESUMO

The development of efficient, stable, and visible-light-responsive photocatalysts is crucial to address the pollution of water bodies by toxic heavy metal ions and organic antibiotics. Herein, a series of LaNi1-xFexO3/g-C3N4 heterojunction photocatalysts are prepared by a simple wet chemical method. Moreover, LaNi0.8Fe0.2O3/g-C3N4 composites are characterized by various methods, including structure, morphology, optical, and electrochemical methods and tetracycline degradation and photocatalytic reduction of Cr(VI) under visible light irradiation. Then, the photocatalytic performance of as-prepared LaNi0.8Fe0.2O3/g-C3N4 composites is evaluated. Compared with pure LaNi0.8Fe0.2O3 and g-C3N4, the LaNi0.8Fe0.2O3/g-C3N4 composite photocatalysts exhibit excellent photocatalytic performance due to synergy of doping and constructing heterojunctions. The results show that the doping of Fe ions can increase the concentration of oxygen vacancies, which is ultimately beneficial to the formation of electron traps. Moreover, the type-II heterojunction formed between LaNi0.8Fe0.2O3 and g-C3N4 effectively strengthens the separation and transfer of photoinduced carriers, thereby promoting photocatalytic activity. Furthermore, the photocatalytic activity of the LaNi0.8Fe0.2O3/g-C3N4 photocatalyst remains almost unchanged after three cycles, indicating long-term stability. Ultimately, the photocatalytic mechanism of the LaNi0.8Fe0.2O3/g-C3N4 composites is proposed.


Assuntos
Antibacterianos , Tetraciclina , Catálise , Luz
2.
Clin Exp Pharmacol Physiol ; 49(12): 1270-1280, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36054718

RESUMO

Gastric cancer (GC) is one of the most prevalent malignancies of the digestive tract. Ginsenoside Rh1 was reported to exert effects on GC. The current study set out to explore the mechanism underlying Ginsenoside Rh1 effects on GC. With oxaliplatin (OXA) serving as the positive control, human GC cells AGS were treated with 0, 10, 25, 50, 74, or 100 µM of ginsenoside Rh1 for 48 h. Proliferation, migration, invasion, and apoptosis were subsequently assessed by means of MTT, scratch test, Transwell, and TUNEL, respectively. AGS cells were further jointly treated with Rh1 and the TGF-ß/Smad pathway activator Kartogenin, followed by detection of TGF-ß/Smad pathway effects on AGS biological behaviours. Moreover, TGF-ß/Smad pathway activation was detected with a Western blot assay. Furthermore, xenograft tumour models were established and tumour growth was recorded. Ki-67 expression patterns and apoptosis were detected with immunohistochemistry and TUNEL, respectively. In vitro, Ginsenoside Rh1 repressed AGS cell proliferation, migration, and invasion, and further promoted apoptosis, with a concentration of 50 µM Rh1 exerting the equivalent effects as OXA. In vivo, Ginsenoside Rh1 inhibited GC proliferation and induced tumour cell apoptosis. Mechanistically, Ginsenoside Rh1 reduced TGF-ß1 and TGF-ß2 levels and Smad2 and Smad3 phosphorylation levels. Collectively, our findings highlighted that ginsenoside Rh1 inhibited GC cell growth and tumour growth in xenograft tumour models via inhibition of the TGF-ß/Smad pathway.


Assuntos
Ginsenosídeos , Neoplasias Gástricas , Animais , Humanos , Camundongos , Movimento Celular , Ginsenosídeos/farmacologia , Camundongos Nus , Transdução de Sinais , Neoplasias Gástricas/metabolismo , Fator de Crescimento Transformador beta1/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Proteínas Smad/metabolismo
3.
Front Public Health ; 10: 925293, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36276394

RESUMO

Aim: This study investigates the trend in general obesity and abdominal obesity in US adults from 2001 to 2018. Methods: We included 44,184 adults from the nine cycles of the continuous NHANES (2001-2002, 2003-2004, 2005-2006, 2007-2008, 2009-2010, 2011-2012, 2013-2014, 2015-2016, and 2017-2018). The age-adjusted mean body mass index and waist circumference were calculated, and the sex-specific annual change was estimated by the survey cycle. We used the weighted sex-specific logistic regression models to analyze the prevalence of general obesity and abdominal obesity from 2001 to 2018. The weighted adjusted odds ratio (OR) with a 95% confidence interval (CI) was calculated. Results: Our study showed that general obesity and abdominal obesity account for about 35.48 and 53.13% of the US population. From 2001-2002 to 2017-2018, the age-adjusted prevalence of general obesity increased from 33.09 to 41.36% in females and from 26.88 to 42.43% in males. During 2001-2018, the age-adjusted prevalence of abdominal obesity increased from 57.58 to 67.33% in females and from 39.07 to 49.73% in males. A significant time-dependent increase was observed in the prevalence of general obesity (adjusted OR, 1.007; 95% CI 1.005-1.009, P < 0.001) and abdominal obesity (adjusted OR, 1.006; 95% CI, 1.004-1.008; P < 0.001). Conclusion: General obesity and abdominal obesity are a heavy health burden among US adults, and the increasing trend remains in both males and females from 2001 to 2018.


Assuntos
Obesidade Abdominal , Obesidade , Masculino , Feminino , Humanos , Obesidade Abdominal/epidemiologia , Inquéritos Nutricionais , Obesidade/epidemiologia , Circunferência da Cintura , Índice de Massa Corporal
4.
J Clin Hypertens (Greenwich) ; 24(3): 320-328, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35188335

RESUMO

This study aims to investigate the association between waist circumference and the development of hypertension based on a nationwide cohort Chinese population. A total of 5330 individuals free of hypertension at baseline were collected from the China Health and Retirement Longitudinal Study. The association between waist circumference and the development of hypertension was analyzed by an adjusted cox regression model and visualized by restricted cubic splines. Further, we applied the supervised machine learning methods to evaluate the importance of multiple variates for new-onset hypertension. Additionally, the robustness of the association was assessed by a subgroup analysis. A total of 1490 individuals (28.0%) developed hypertension during a mean follow-up of 3.32 years. The new-onset hypertension was more observed in those with increased waist circumference (P for trend < .001). In the fully adjusted Cox regression, each 10 cm increase of waist circumference would result in an 18% elevated risk of hypertension. The random forest method and the Extreme Gradient Boosting method revealed waist circumference as an important feature to predict the development of hypertension. The sensitivity analysis indicated a consistent trend between waist circumference and new-onset hypertension in all BMI categories. This study suggested high waist circumference as an independent risk factor for new-onset hypertension based on a nationwide cohort of Chinese adults aged ≥45 years old. Our results supported that waist circumference should be routinely measured.


Assuntos
Hipertensão , Adulto , Índice de Massa Corporal , China/epidemiologia , Humanos , Hipertensão/diagnóstico , Hipertensão/epidemiologia , Hipertensão/etiologia , Estudos Longitudinais , Pessoa de Meia-Idade , Aposentadoria , Fatores de Risco , Circunferência da Cintura
5.
World J Gastroenterol ; 28(37): 5420-5443, 2022 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-36312839

RESUMO

BACKGROUND: Colorectal cancer (CRC) is one of the most common malignant tumors worldwide. The identification of novel diagnostic and prognostic biomarkers for CRC is a key research imperative. Immunohistochemical analysis has revealed high expression of centromere protein K (CENPK) in CRC. However, the role of CENPK in the progression of CRC is not well characterized. AIM: To evaluate the effects of knockdown of CENPK and overexpression of Cullin 4A (CUL4A) in RKO and HCT116 cells. METHODS: Human colon cancer samples were collected and tested using a human gene expression chip. We identified CENPK as a potential oncogene for CRC based on bioinformatics analysis. In vitro experiments verified the function of this gene. We investigated the expression of CENPK in RKO and HCT116 cells using quantitative polymerase chain reaction (qPCR), western blot, and flow cytometry. The effect of short hairpin RNA (shRNA) virus-infected RKO cells on tumor growth was evaluated in vivo using quantitative analysis of fluorescence imaging. To evaluate the effects of knockdown of CENPK and overexpression of CUL4A in RKO and HCT116 cells, we performed a series of in vitro experiments, using qPCR, western blot, MTT assay, and flow cytometry. RESULTS: We demonstrated overexpression of CENPK in human colon cancer samples. CENPK was an independent risk factor in patients with CRC. The downstream genes FBX32, CUL4A, and Yes-associated protein isoform 1 were examined to evaluate the regulatory action of CENPK in RKO cells. Significantly delayed xenograft tumor emergence, slower growth rate, and lower final tumor weight and volume were observed in the CENPK short hairpin RNA virus infected group compared with the CENPK negative control group. The CENPK gene interference inhibited the proliferation of RKO cells in vitro and in vivo. The lentivirus-mediated shRNA interference of CENPK inhibited the proliferation of RKO and HCT116 colon cancer cells, with overexpression of the CUL4A. CONCLUSION: We indicated a potential role of CENPK in promoting tumor proliferation, and it may be a novel diagnostic and prognostic biomarker for CRC.


Assuntos
Neoplasias do Colo , Neoplasias Colorretais , Humanos , Lentivirus/genética , RNA Interferente Pequeno/genética , Proteínas Culina/genética , Proteínas Culina/metabolismo , Proliferação de Células/genética , Neoplasias Colorretais/patologia , Linhagem Celular Tumoral , Neoplasias do Colo/patologia , Regulação Neoplásica da Expressão Gênica , Interferência de RNA , Movimento Celular , Proteínas de Ligação a DNA/genética , Proteínas Nucleares/metabolismo
6.
J Colloid Interface Sci ; 611: 684-694, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34974228

RESUMO

Interfacial design and the co-catalyst effect are considered to be effective to achieve separation and transport of photogenerated carriers in composite photocatalysts. In this study, a Z-scheme heterojunction was successfully combined with a co-catalyst to achieve a highly efficient LaNiO3/g-C3N4/MoS2 photocatalyst. MoS2 flakes were loaded on a hybrid material surface, which was formed by LaNiO3 nanocubes embedded on layered g-C3N4, and a good heterostructure with multiple attachment sites was obtained. Experimental studies confirmed that the Z-scheme heterojunction completely preserves the strong redox ability of the photogenerated electrons and holes. As a cocatalyst, MoS2 further promoted interfacial charge separation and transport. The synergistic effect of the Z-scheme heterojunction and co-catalyst effectively realized the transfer of photogenerated carriers from "slow transfer" to "high transfer" and promoted water decomposition and pollutant degradation. Results revealed that under simulated sunlight irradiation, LaNiO3/g-C3N4/MoS2 composites exhibit superior hydrogen evolution of 45.1 µmol h-1, which is 19.1 times that of g-C3N4 and 4.9 times that of LaNiO3/g-C3N4, respectively. Moreover, the LaNiO3/g-C3N4/MoS2 Z-scheme photocatalyst exhibited excellent photocatalytic performance for antibiotic degradation and heavy-metal ion reduction under visible light. This study might provide some insights into the development of photocatalysts for solar energy conversion and environmental remediation.


Assuntos
Grafite , Molibdênio , Dissulfetos , Lantânio , Compostos de Nitrogênio
7.
Kaohsiung J Med Sci ; 37(5): 419-426, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33305507

RESUMO

This study aimed to explore whether APOC1 expression has a function in the biological behavior of clear cell renal cell carcinoma (ccRCC) cells and its possible mechanism. Bioinformatics analysis of data TCGA and OnComine was conducted to explore the expression pattern and prognostic value of APOC1, as well as the relationship between APOC1 expression and clinical indicators. Loss- and gain- of APOC1 function assays were carried out to assess the biological functions of APOC1. Western blotting was applied to detect protein expression. We revealed that APOC1 was upregulated in ccRCC tissues. APOC1 expression was related to gender, grade, pathologic-T, pathologic-stage, and pathologic-M in patients with ccRCC. Meanwhile, Kaplan-Meier analysis evidenced that the high APOC1 expression indicated unfavorable outcomes of ccRCC. Functional experiments in vitro revealed that upregulation of APOC1 in UT33A cells promoted cell proliferation, invasion, and migration, while downregulation of APOC1 in 786-O cells had the opposite effect. Furthermore, epithelial mesenchymal transition (EMT) was activated in cells with upregulated APOC1 but inhibited in cells with down-regulated APOC1. Collectively, our data suggested that APOC1 was overexpressed in ccRCC cells and promoted the malignant biological behaviors and EMT of ccRCC cells.


Assuntos
Apolipoproteína C-I/biossíntese , Biomarcadores Tumorais/metabolismo , Carcinoma de Células Renais/diagnóstico , Carcinoma de Células Renais/metabolismo , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Neoplasias Renais/diagnóstico , Neoplasias Renais/metabolismo , Idoso , Linhagem Celular Tumoral , Proliferação de Células , Sobrevivência Celular , Transição Epitelial-Mesenquimal , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Fenótipo , Prognóstico , RNA Interferente Pequeno/metabolismo , Análise de Regressão , Regulação para Cima
9.
Molecules ; 10(1): 238-43, 2005 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-18007291

RESUMO

The palladium-catalyzed cross-coupling carbonyl insertion reaction between 3,7-bis(N,N-dimethylamino)-10H-dibenz[b,e]iodinium iodide (1) and alcohols or amines 2 is described. Some new amides and esters 3 containing an active iodo functional group have been prepared in 65-91% yields.


Assuntos
Álcoois/síntese química , Aminas/síntese química , Carbono/química , Compostos Heterocíclicos/síntese química , Compostos de Iodo/síntese química , Paládio/farmacologia , Álcoois/química , Aminas/química , Catálise/efeitos dos fármacos , Compostos Heterocíclicos/química , Compostos de Iodo/química , Modelos Biológicos
10.
Forensic Sci Int ; 173(2-3): 210-3, 2007 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-17320329

RESUMO

Allele frequency data for 15 STR loci, namely D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA, were obtained from a sample of 258 healthy unrelated individuals of Salar population in China. All loci were in Hardy-Weinberg equilibrium. After comparing, significant differences were found between the Salar and Miao, Uigur, Han, Korean, Belgian, Byelorussian population at some loci. The results demonstrate that the loci are useful for forensic human identification and parentage testing.


Assuntos
Etnicidade/genética , Genética Populacional , Polimorfismo Genético , Sequências de Repetição em Tandem , China , Impressões Digitais de DNA , Frequência do Gene , Humanos , Reação em Cadeia da Polimerase
11.
J Org Chem ; 68(21): 8268-71, 2003 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-14535817

RESUMO

8O-methylaphanorphine was synthesized from 4-methoxyphenylacetaldehyde in 36% overall yield and in nine steps, featuring the formation of ring B via a Friedel-Crafts alkylative cyclization with the concomitant stereospecific introduction of the benzylic quaternary carbon center. The current work constitutes an efficient enantioselective formal synthesis of 3-benzazepine marine alkaloid (-)-aphanorphine.


Assuntos
Acetaldeído/análogos & derivados , Acetaldeído/química , Hidrocarbonetos Aromáticos com Pontes/síntese química , Pirróis/síntese química , Hidrocarbonetos Aromáticos com Pontes/química , Estrutura Molecular , Pirróis/química , Estereoisomerismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA