Comparison of facial soft tissue changes after treatment with 3 different functional appliances.

INTRODUCTION: This study aimed to evaluate the effects of 3 different fixed or removable functional appliances on the soft tissue changes in patients with Class II Division 1 malocclusion using 3-dimensional images. METHODS: A total of 60 patients with Class II Division 1 malocclusion (38 girls and 22 boys; mean age, 12.35 ± 1.01 years) were treated with 3 different functional appliances, namely, Twin-block (TB) (group TB, n = 20), mono-block (MB) (group MB, n = 20), or Herbst (H) (group H, n = 20) for 9.55 ± 1.46 months. Three-dimensional photographs of each patient were taken at the baseline and the end of the therapy with a 3dMD Face system (3dMD, Atlanta, Ga). Statistical analyses were performed using the dependent samples t test, 1-way analysis of variance, Kruskal-Wallis, Mann-Whitney U, and Wilcoxon signed rank tests. RESULTS: No statistically significant differences were detected for soft tissue changes except for the lower facial width found, at least in group H (P <0.05). Volumetric differences in the mandible were similar between the groups (P >0.05). A statistically significant decrease in total facial height and an increase in convexity angle and facial depth were detected in all groups after treatment compared with the baseline (P <0.05). The upper and lower facial height, lower lip height, and nasal width were statistically significantly increased in the TB and MB groups after treatment compared with the baseline (P <0.05) and similar to those in group H (P >0.05). CONCLUSIONS: TB, MB, and H appliances may promote the facial soft tissue profile, including volumetric improvement, in the mandibular region.

Treatment of Class II malocclusion with mandibular skeletal anchorage.

INTRODUCTION: The aim of this case report was to present the dentofacial changes obtained with bone anchorage in a Class II patient with moderate to severe crowding. METHODS: A boy, aged 14.5 years, with a dolichofacial type, convex profile, and skeletal and dental Class II relationships was examined. After evaluation, functional treatment with bone anchorage and 4 first premolar extractions was decided as the treatment approach. Miniplates were placed on the buccal shelves of the mandibular third molars. The hook of the anchor was revealed from the first molar level. After surgery, the 4 first premolars were extracted to retract the protrusive mandibular incisors. The maxillary and mandibular first molars were banded, and a lip bumper was inserted to apply elastics and to help distalize the maxillary first molars. Orthodontic forces of 300 to 500 g were applied immediately after placement, originating from the miniscrews to the hooks of the appliance to advance the mandible. RESULTS: After 20 months of treatment, the patient had a dental and skeletal Class I relationship, the mandible was advanced, the maxilla was restrained, and overjet was decreased. CONCLUSIONS: The combination of a bone anchor, Class II elastics, and an inner bow is a promising alternative to functional treatment, along with extractions, in Class II patients.

The Role of Ideal Angles, Ratios, and Divine Proportions in Aesthetic Evaluation of Adolescents.

OBJECTIVES: The aim of our study was to investigate whether the parameters of ideal angles, ratios, and divine proportions that have been previously mentioned in the literature influence the aesthetic evaluation of Turkish adolescents. MATERIALS AND METHODS: A total of sixty patients (30 males and 30 females) were enrolled in this study. Ages of the subjects ranged from 9 to 17. Pre-treatment extra-oral photographs were taken to evaluate facial aesthetics. Two distinct panels consisting of 50 orthodontists and 50 laypersons were created for scoring the photographs of the patients. Scoring was performed using the VAS scale. Twenty-seven ratios and 19 divine proportions were measured in frontal photographs, and 26 angles were measured in profile photographs. RESULTS: Pearson correlation was used to determine the relationship between the photograph analysis measurements and VAS scores, and then regression analyses were performed to disclose to what extent significant values may warrant the term beauty. CONCLUSION: As a result of our investigations, none of the golden proportions was associated with facial aesthetics according to both orthodontists and laypersons. According to other angles and ratios that were measured, it was determined that orthodontists noticed the sagittal position of the lower jaw, the distance between the eyes and length of the face, whereas laypersons noticed only the distance between the eyes and length of the face. LEVEL OF EVIDENCE V: This journal requires that the authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Microcomputed tomography evaluation of white spot lesion remineralization with various procedures.

INTRODUCTION: The aim of this study was to use microcomputed tomography to evaluate the effects on white spot lesions of 3 remineralizing agents compared with artificial saliva (Inonu University, Malatya, Turkey). The agents were GC Tooth Mousse (GC International, Itabashi-ku, Tokyo, Japan), 50-ppm sodium fluoride solution (Inonu University, Malatya, Turkey), and Clinpro 5000 (3M ESPE Dental Products (St Paul, Minn). The experimental and control teeth were stored in artificial saliva. METHODS: Forty-four extracted premolars were divided into 4 groups of 11 teeth each (3 experimental groups and 1 control group). After white spot lesions were created on the teeth, a remineralizing agent was applied. Microcomputed tomography scanning was performed at the following times: T0 (sound enamel), T1 (day 0, when the white spot lesion was formed), T2 (day 15), and T3 (day 30). Volume, depth, surface area, and mineral density changes of the white spot lesions were evaluated at different time points using CTAn software (SkyScan; Bruker, Kontich, Belgium). RESULTS: GC Tooth Mousse and Clinpro 5000 improved all measurements after 30 days. However, Clinpro 5000 was not as effective in reducing lesion depth as it was in the other parameters. The artificial saliva group and the 50-ppm sodium fluoride solution did not show significant effects in the regression of the white spot lesions at the end of the 30-day experiment. CONCLUSIONS: GC Tooth Mousse and Clinpro 5000 were more effective in remineralization of white spot lesions than sodium fluoride solution and artificial saliva. They can be preferred for use clinically. Microcomputed tomography is a novel and effective method that shows promise in accurately evaluating white spot lesions and remineralization.

Micro-CT evaluation of microleakage under orthodontic ceramic brackets bonded with different bonding techniques and adhesives.

AIM: The aim of this study was to evaluate microleakage under orthodontic ceramic brackets bonded with direct and different indirect bonding techniques and adhesives using micro-computed tomography. MATERIALS AND METHODS: A total of 30 human maxillary premolars were randomly separated into five groups with six teeth in each group. In group I, teeth were bonded directly with Transbond XT (3M Unitek). In group II, group III, group IV, and group V, teeth were bonded through an indirect technique with Custom I.Q. (Reliance Orthodontic Products), Sondhi Rapid-Set (3M Unitek), RMbond (RMO), and Transbond IDB (3M Unitek), respectively, following the manufacturer's instructions. Micro-CT system model 1172 of Skyscan (Kontich, Belgium) was used to scan all samples. NRecon (Skyscan) version 1.6, CT-Analyser V.1.11 (Skyscan), and TView (SkyScan, Bvba) software programs were used for microleakage evaluation. Microleakage values between the test groups were assessed using the Kruskal-Wallis test, while the Wilcoxon signed rank test was used for within-group comparisons. The level of significance was set at P < 0.05. RESULTS: According to the Kruskal-Wallis analysis of variance test, there were no significant differences among the tested groups, with regard to volume and percentage (microleakage/region of interest × 100) of microleakage values (P < 0.05). The Wilcoxon signed rank test showed that coronal microleakage volume and percentage values significantly differed for RMbond and Transbond IDB groups. LIMITATIONS: In the study, only ceramic brackets were used and microleakage into mini gaps did not show up on the micro-CT image because 50% silver nitrate solution could not penetrate into mini gaps which are smaller than silver nitrate particles. CONCLUSION: Use of direct and indirect bonding techniques with different adhesives did not significantly affect the amount of microleakage.

Cytotoxicity Evaluation of Bioresorbable Fixation Screws on Human Gingival Fibroblasts and Mouse Osteoblasts by Real-Time Cell Analysis.

PURPOSE: To evaluate the effects of bioresorbable fixation screws (BFSs) on human gingival fibroblast (HGF) and mouse osteoblast (MC3T3-E1) cell viability. MATERIALS AND METHODS: The KLS Martin SonicPins Rx, Synthes RapidSorb Cortex Screws, and Inion CPS Bioabsorbable Fixation System each were incubated in Dulbecco's Modified Eagle Medium for 72 hours according to ISO 10993-5 standards. A real-time cell analyzer was used to evaluate cell survival. After seeding 200-µL cell suspensions in the wells of an E-plate View 96, HGF and MC3T3-E1 cells were treated with the bioactive components released by the bioresorbable materials and monitored every 15 minutes for 96 hours. Statistical significance was determined using 1-way analysis of variance and Tukey-Kramer tests. RESULTS: There were significant differences in the HGF responses to the untreated control conditions and the Synthes (P < .01), Inion (P < .05), and KLS Martin (P < .05) treatments over 48 hours. The Synthes (P < .01) and Inion (P < .01) treatments produced lower HGF cell index values than the untreated control at 72 hours, whereas the KLS Martin treatment did not. When left to elute for 96 hours, there were no significant differences in values among the control and study groups for HGFs (P > .05). All tested BFSs decreased cell survival rates of M3T3C1 cells for 48 hours (P < .01), 72 hours (P < .001), and 96 hours (P < .001). CONCLUSION: Differences in the sensitivities of the 2 tested cell lines to the different BFSs might be the result of the different materials used to manufacture the screws. These results provide fundamental knowledge and new insights for the future design and development of new biocompatible BFSs for oral and maxillofacial surgery.

Real-time cell analysis of the cytotoxicity of orthodontic mini-implants on human gingival fibroblasts and mouse osteoblasts.

INTRODUCTION: The aim of this study was to evaluate the cytotoxic effects of orthodontic mini-implants on gingival fibroblasts and osteoblasts. METHODS: The orthodontic mini-implants used in this study were Orthodontic Mini Implant (Leone, Florence, Italy), MTN (MTN, Istanbul, Turkey), AbsoAnchor (Dentos, Daegu, South Korea), IMTEC Ortho (3M Unitek, IMTEC, Ardmore, Okla), VectorTAS (Ormco, Glendora, Calif). The materials were incubated in Dulbecco's modified eagle's culture medium for 72 hours according to ISO 10993-5 standards (surface area-to-volume ratio of the specimen to cell-culture medium, 3 cm(2)/mL). A real-time cell analyzer (xCELLigence, Roche Applied Science, Mannheim, Germany; ACEA Biosciences, San Diego, Calif) was used to evaluate cell survival. After seeding 200 µL of the cell suspensions into the wells of the E-plate 96, gingival fibroblasts were treated with bioactive components released by the metallic materials and monitored every 15 minutes for 190 hours. For the proliferation experiments, the statistical analyses used were 1-way analysis of variance and Tukey-Kramer multiple comparisons tests. RESULTS: There was no significant differences between the human gingival fibroblast cell indexes of the control and study groups (P >0.05). When evaluated at 27 and 96 hours, only the VectorTAS mini-implants showed statistically significant decreases in the M3T3 cell index (P <0.001) compared with the control group. No significant differences were found among the control and all study groups (P >0.05). Furthermore, the Leone and MTN mini-implants showed statistically significant decreases (P <0.001) at 190 hours. Also, the VectorTAS mini-implants demonstrated a significant decline (P <0.05) at the same time in the M3T3 cell index. CONCLUSIONS: These findings provide fundamental knowledge and new insights for future design and development of new biocompatible titanium alloys for orthodontic mini-implants and temporary anchorage devices.

Comparison of the outcomes of the lower incisor extraction, premolar extraction and non-extraction treatments.

The aim of this retrospective study was to evaluate the treatment outcome of lower incisor extraction and to compare it with premolar extraction and non-extraction treatment. The sample consisted of 60 subjects with Class I malocclusion and moderate crowding. The sample was separated into three groups: extraction of a lower incisor group, extraction of a four first premolar group and a non-extraction group. All groups involved 13 girls and 7 boys with a total of 20 patients. The Peer assessment rating (PAR) index was applied to a patient's pre-treatment (T1) and post-treatment (T2) dental casts. T1 dental casts were also used for determining Bolton discrepancy. One-way analysis of variance and post hoc Tukey HSD tests were used for statistical analysis. For the mean percentage PAR score reduction for each group, there was one significant difference seen between the lower incisor extraction group and the non-extraction group (P = 0.047). For the mean anterior ratios, there were significant differences among premolar extraction group versus non-extraction group (P = 0.042) and non-extraction group versus lower incisor extraction group (P = 0.000). For the mean overall ratios, there were significant differences among the premolar extraction group versus lower incisor extraction group (P = 0.048) and the non-extraction group versus lower incisor extraction group (P = 0.001). Orthodontic treatment without extraction has a better treatment outcome than the four-first premolar extraction and single lower incisor extraction protocols in Class I cases with moderate to severe mandibular anterior crowding.

Effects of teriparatide on bone formation in rats with experimentally induced premaxillary expansion.

OBJECTIVE: This study aimed to evaluate the effects of systemic teriparatide on sutural bone formation after premaxillary suture expansion in rats. MATERIAL AND METHODS: Twenty Wistar male rats (8-10 weeks old) were randomly divided into two groups, namely, control (C, n=10) and teriparatide (T, n=10). An expansion force was applied to the maxillary incisors using helical spring for a seven-day expansion period, for both groups. On the eighth day, the rats were kept for a seven-day consolidation period, and then 60 µg/kg teriparatide (once a day) was administered to group T subcutaneously for seven days. Then, all the rats were sacrificed, and histological sections were stained with hemotoxylin-eosin for examination. Anti-osteonectin, anti-osteocalcin, anti-Vascular endothelial growth factor (VEGF) and anti-transforming growth factor beta (TGF-ß) were evaluated by immunohistochemical analysis in the midpalatal suture area. RESULTS: Histologically, the newly formed bone tissue was observed to be larger in group T than in group C. The number of immunoreactive osteoblasts for osteonectin, osteocalcin and VEGF antibodies was significantly higher in group T than in group C (p = 0.0001). The TGF-ß antibody showed a mild reaction in group T, but did not reach significance in comparison with group C (p ˃ 0.05). CONCLUSION: Systemic teriparatide application following the premaxillary expansion of the suture area may stimulate bone formation and add to the consolidation of the expansion in rats by regulating osteonectin, osteocalcin and VEGF.

Polymerization capacity of orthodontic composites analyzed by Fourier transform infrared spectroscopy.

INTRODUCTION: The aim of this in-vitro study was to analyze the polymerization capacity of 5 orthodontic composites by determining the degree of monomer conversion (DC). METHODS: Fourier transform infrared spectroscopy was used to evaluate the DC of the orthodontic composites immediately after polymerization and after storage in artificial saliva at 37°C ± 1°C for 30 days. The resin-based adhesive composites investigated were Bisco Ortho (Bisco, Schaumburg, Ill), Heliosit Orthodontics (Ivoclar, Schaan, Liechtenstein), Kurasper F (Kuraray, Okayama, Japan), Light Bond (Reliance Orthodontic Products, Itasca, Ill), and Transbond XT (3M Unitek, Monrovia, Calif), cured with Elipar FreeLight 2 (3M ESPE, St Paul, Minn) for the testing of the DC values. Fifty cylindrical specimens were manufactured in molds. The data were analyzed by 2-factor analysis of variance (ANOVA) and Tukey HSD test. RESULTS: According to 2-way ANOVA, the DC was significantly influenced by composite type (P <0.05); after 30 days, there were no differences among the composite types for the DC. The interaction of orthodontic composites and time played a statistically significant role in the DC (P <0.05), but there was no statistically significant influence of time for the DC (P >0.05). CONCLUSIONS: The DC was found to change according to composite materials used, and Bisco Ortho showed the most DC performance. The DC of orthodontic composites is a complex process that is affected not only by inorganic filler content of the composite but also the monomer type and many other factors. Sufficient DC values of 5 commercially available orthodontic composites can be achieved with a new-generation light-emitting diode curing light.

Real-time cell analysis of the cytotoxicity of the components of orthodontic acrylic materials on gingival fibroblasts.

INTRODUCTION: The aim of this study was to evaluate the cytotoxicity of 3 orthodontic acrylic materials and 2 manipulation methods. METHODS: The orthodontic acrylic materials Orthocryl EQ (Dentaurum, Ispringen, Germany), Orthoplast (Vertex Dental, Zeist, The Netherlands), and O-80 (Imicryl, Konya, Turkey) were prepared with 2 polymerization methods (doughing and spray on). Totally, 60 cylinders (5 × 2 mm), fabricated by using a different acrylic and method, were divided into 6 groups. Gingival fibroblasts were isolated from gingival connective tissue of systemically healthy subjects. Materials were incubated in Dulbecco's modified eagle's medium culture medium (Biological Industries, Beit Haemek, Israel) for 72 hours according to ISO 10993-5 standards (surface area to volume ratio of the specimen to cell-culture medium: 3 cm(2)/mL). Gingival fibroblasts were maintained with Dulbecco's modified eagle medium containing 10% fetal bovine serum. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Mannheim, Germany, and ACEA Biosciences, San Diego, Calif) was used to evaluate cell survival. After seeding 200 µL of the cell suspensions into the wells (20,000 cells/well) of the E-plate 96, gingival fibroblasts were treated with bioactive components released by the acrylic materials (1/1 and 1/2 dilutions) and monitored every 15 minutes for 121 hours. For the proliferation experiments, the statistical analyses used were 1-way analysis of variance (ANOVA) and Tukey-Kramer multiple comparisons tests. RESULTS: There was no significant difference between the cell indexes of the control and study groups for the 1/1 and 1/2 dilutions at 21 and 32 hours. When evaluated at 68 hours, all 1/2 dilutions of acrylic materials showed statistically insignificant differences (P >0.05) except for Orthoplast (P <0.05). But all acrylic materials were different from the control group in the 1/1 dilutions (P <0.001). At 121 hours, all test groups were significantly different from the untreated control group (P <0.001). CONCLUSIONS: The results indicate that the long cycle increased the cytotoxicity of the tested materials, and there was no significant difference between the spray-on and doughing methods on cytotoxicity.

Maxillary and mandibular mesiodistal tooth sizes among different malocclusions in a sample of the Turkish population.

The purpose of this study was to establish normative data for mesiodistal tooth crown dimensions with respect to malocclusions and gender differences in Turkish sample. The subjects were randomly selected and assigned to three malocclusion groups according to Angle's classification. Each group consisted of 100 individuals between the ages of 13 and 18 years with the following distribution: Class I, 42 males and 58 females; Class II, 52 males and 48 females; and Class III, 51 males and 49 females. An electronic digital calliper was used to measure the mesiodistal tooth width from the right second permanent molar to the left second permanent molar on both upper and lower study casts. For statistical evaluation, one- and two-way analyses of variance and post hoc Tukey's honestly significant difference (HSD) tests were performed. There were statistically significant differences for the maxillary canine (P < 0.001), first premolar (P < 0.05), second molar (P < 0.05), and mandibular canine (P < 0.01) for males, and for all maxillary teeth and the mandibular central (P < 0.05), canine (P < 0.001), and first premolar (P < 0.05) teeth in females among the malocclusion groups. When Angle's classification was evaluated, significant differences were determined, except for the first and second mandibular molars. All mesiodistal widths were also found to be statistically different according to gender dimorphism. A significant relationship was found between mesiodistal tooth size, Angle's classification, and gender. Therefore, tooth dimensions may play a crucial role in treatment planning and in achieving satisfactory interdigitation of the upper and lower dentition following the completion of orthodontic treatment.

Does mini-implant-supported rapid maxillary expansion cause less root resorption than traditional approaches? A micro-computed tomography study.

OBJECTIVE: This study aimed to evaluate the volume, amount, and localization of root resorption in the maxillary first premolars using micro-computed tomography (micro-CT) after expansion with four different rapid maxillary expansion (RME) appliances. METHODS: In total, 20 patients who required RME and extraction of the maxillary first premolars were recruited for this study. The patients were divided into four groups according to the appliance used: miniimplant- supported hybrid RME appliance, hyrax RME appliance, acrylic-bonded RME appliance, and full-coverage RME appliance. The same activation protocol (one activation daily) was implemented in all groups. For each group, the left and right maxillary first premolars were scanned using micro-CT, and each root were divided into six regions. Resorption craters in the six regions were analyzed using special CTAn software for direct volumetric measurements. Data were statistically analyzed using Kruskal-Wallis one-way analysis of variance and Mann-Whitney U test with Bonferroni adjustment. RESULTS: The hybrid expansion appliance resulted in the lowest volume of root resorption and the smallest number of craters (p < 0.001). In terms of overall root resorption, no significant difference was found among the other groups (p > 0.05). Resorption was greater on the buccal surface than on the lingual surface in all groups except the hybrid appliance group (p < 0.05). CONCLUSIONS: The findings of this study suggest that all expansion appliances cause root resorption, with resorption craters generally concentrated on the buccal surface. However, the mini-implant-supported hybrid RME appliance causes lesser root resorption than do other conventional appliances.

Does the rhythm and appliance type of rapid maxillary expansion have an effect on root resorption?

OBJECTIVES: To evaluate the volume, amount, and localization of root resorption in the upper first premolars by micro-computed tomography (micro-CT) after three different rapid maxillary expansion appliances and two different activation rhythms. MATERIALS AND METHODS: The patients were divided into three groups; Hyrax, acrylic cap splint (ACS), and full coverage acrylic bonded (FCAB) appliances. Each group was then divided into the following two subgroups: rapid maxillary expansion (RME) and semirapid maxillary expansion (SRME). After expansion was completed, the appliances were stabilized for 12 weeks during the retention period. For each group, 10 premolars (for a total of 60 premolars) were scanned with the micro-CT (SkyScan). The reconstructed 3D images of each root sample were divided into six regions. The resorption craters on these six different root surfaces were analyzed by special CTAn (SkyScan) software for direct volumetric measurements. Kruskal-Wallis one-way analysis of variance and Mann-Whitney U tests were used for statistical analysis. RESULTS: The total volume of root resorption was less with FCAB than with ACS and Hyrax (P < .001). In all groups, a greater volume of resorption was found on the buccal surface than on the lingual surface (P < .001). No significant differences were found between the RME and SRME groups (P > .05). CONCLUSIONS: All expansion appliances caused root resorption in the upper first premolar teeth, but FCAB may be safer in terms of root resorption. The resorption craters were generally concentrated on the buccal surface. There was no effect of activation rhythm on root resorption.

Three-dimensional soft tissue evaluation after rapid maxillary expansion and mandibular midline distraction osteogenesis.

OBJECTIVES: To evaluate the effects of rapid maxillary expansion (RME) and mandibular midline distraction osteogenesis (MMDO) on facial soft tissues using three-dimensional (3D) images. MATERIALS AND METHODS: A total of 20 patients (average age 15.86 ± 2.17 years) were treated with RME and MMDO using tooth-borne distractors. Three-dimensional photographs of each patient were taken with a stereophotogrammetry system at baseline (T0), at the end of the distraction period (T1), and at the end of the consolidation period (T2). All data were analyzed using a dependent-samples t-test at a significance level of 5%. RESULTS: Total and lower face height increased after MMDO (P < .05). Nasal and mouth width increased after RME as compared with baseline (P < .05). The labiomental angle increased at T1 and decreased at T2 (P < .05). After MMDO, the convexity angle increased while the mandibular angle decreased (P < .05). Upper and lower lip angles increased after RME (P < .05). The distance from the lower lip to the E plane increased after MMDO and decreased after RME (P < .05). CONCLUSIONS: The MMDO and RME procedures provide an efficient nonextraction treatment alternative for transverse maxillomandibular deficiency. MMDO may improve the facial soft tissue profile in the transverse and vertical axis of the mandibular region.

Borate and boric acid supplementation of drinking water alters teeth and bone mineral density and composition differently in rabbits fed a high protein and energy diet.

The reported beneficial effects of boron on mineralized tissues in animals and humans vary. Thus, a study was performed to assess whether the variability was the result of different forms of boron supplementation, method of supplementation, and increased adiposity of the rabbit experimental model. Thirty-one female New Zealand White rabbits, (aged 8 months, 2-2.5 kg weight) were fed a grain-based high energy diet containing 11.76 MJ/kg (2850 kcal/kg) and 3.88 mg boron/kg. The rabbits were randomly divided into four treatment groups: Control group was not supplemented with boron (n:7; C), and three groups supplemented with 30 mg boron/L in drinking water in the forms of borax decahydrate (Na2O4B7 10H2O, n:10; BD), borax anhydrous (Na2O4B7, n:7; Bah) or boric acid (H2BO3, n:7; BA). Cone beam micro computed tomographic (micro-CT), histological and elemental analysis was used to evaluate the bones/teeth. Results of the experiments demonstrated that boron supplementation had beneficial effects on mineralized tissue but varied with the type of treatment. Mineral density of the femur was increased by the Bah and BA treatments (p < 0.001), but only BA increased mineral density in the tibia (p = 0.015). In incisor teeth, mineral density of dentin was increased by all boron treatments (p < 0.001), and mineral density of enamel was increased by the BD and Bah treatments. Mineral analysis found that all boron treatments increased the boron concentration in tibia and femur. In the tibia, both the BD and Bah treatments decreased the iron concentration, and the BD treatment decreased the magnesium concentration. Sodium and zinc concentrations in the tibia were decreased by the Bah and BA treatments. The boron treatments did not significantly affect the calcium, copper, molybdenum, potassium phosphorus, and sulfur concentrations. The findings show that boron supplementation can have beneficial effects on mineralized tissues in an animal model with increased adiposity, which is a model of increased inflammatory stress. However, this effect varies with the form of boron supplemented, the method of supplementation, and the mineralized tissue examined.

Effects of staining solutions on the discoloration of orthodontic adhesives: an in-vitro study.

INTRODUCTION: Our aim was to evaluate the effects of staining solutions on the discoloration of orthodontic adhesives. METHODS: Six orthodontic adhesives were investigated (Transbond XT [3M Unitek, Monrovia, Calif, USA], Heliosit Orthodontic [Ivoclar Vivadent, Liectenstein], Light Bond [Reliance Orthodontic Products, Itasca, IL, USA], Bisco Ortho [Bisco, Schamburg, IL, USA], Quick Cure [Reliance Orthodontic Products, Itasca, IL, USA], and Filtek Supreme XT [3M ESPE, St Paul, Minn, USA]), and 5 beverages (tea, cola, coffee, red wine, and yogurt). Sixty specimens were prepared. Five specimens from each group were stored in each of the 5 staining solutions. The 5 remaining specimens from each group served as the controls and were stored in distilled water. The specimens were immersed in staining solutions and water at 37°C ± 1°C for 5 days. The test period was 25 days. Before and after the test period, color measurements were carried out with a spectrophotometer, and color changes (ΔE∗) were calculated. Statistical differences were evaluated by using analysis of variance (ANOVA) and the Tukey HSD tests. RESULTS: Adhesive materials, staining agents, and their interactions were found to play statistically significant roles (P < 0.001) in color changes. Among the adhesive materials, the Light Bond water control group consistently showed the lowest ΔE∗ value for all materials, and the Filtek Supreme XT group showed the highest ΔE∗ value for all materials. After the in-vitro experimental process for staining solutions and water, unsatisfactory color stability was observed for the conventional adhesive systems except for Light Bond, Transbond XT, and Bisco Ortho water control group (ΔE∗ > 3.7), respectively. CONCLUSIONS: In esthetically critical areas, discoloration of adhesive materials for fixed orthodontics can cause patient dissatisfaction. Orthodontic composites will discolor from staining beverages during their lifespan.

In-vitro assessment of temperature rise in the pulp during orthodontic bonding.

INTRODUCTION: In this in-vitro study, we evaluated the temperature changes in the pulp chamber during bracket bonding using 4 different light sources. METHODS: Eighty intact extracted maxillary central incisors were used. The teeth were divided into 4 groups of 20 teeth each. Brackets (Mini Twin, Dentaurum, Ispringen, Germany) were bonded with Transbond XT (3M Unitek, Monrovia, Calif) adhesive and light cured with low-intensity halogen light for 40 seconds, high-intensity halogen light for 40 seconds, light-emitting diode (LED) light for 20 seconds, and plasma arc light (PAC) for 6 seconds. Light curing was performed 5 mm from tooth surfaces. A J-type thermocouple wire was positioned in the center of the pulp chamber. The results were analyzed with analysis of variance (ANOVA) and the Tukey HSD test. RESULTS: ANOVA and the Tukey HSD test showed that pulp chamber temperature changes were influenced by the type of light source. All groups showed significant differences between each other (P <0.001). The intrapulpal temperature changes induced by different light sources were the following: high-intensity halogen (6.84 degrees C +/- 2.44 degrees C), low-intensity halogen (4.71 degrees C +/- 0.96 degrees C), LED (2.95 degrees C +/- 1.12 degrees C), and PAC (0.96 degrees C +/- 0.83 degrees C). CONCLUSIONS: High- and low-intensity halogen light induced significantly higher intrapulpal temperature changes than did the LED and PAC. Except for the high intensity halogen light, orthodontic bonding with light-curing units did not exceed the critical 5.5 degrees C rise in temperature reported to produce pulpal damage.

The incidence of canine transmigration and tooth impaction in a Turkish subpopulation.

The purpose of this study was to determine the incidence of transmigrated maxillary and mandibular canine teeth and also of the other impacted teeth in a Turkish subpopulation. Five thousand consecutive panoramic radiographs taken of patients who attended the Department of Oral Diagnosis and Radiology, Selcuk University, for routine examination between 2005 and 2007 were examined to identify incidences of transmigrated and impacted teeth. Demographic information of all patients was also recorded. Transmigrations were categorized according to the classification of Mupparapu. The effect of age, gender, and sidedness (left or right side) on the recorded abnormality was evaluated using a t-test. The incidence of transmigrated upper and lower canine teeth was found to be 0.34 per cent [17 patients, 12 females/5 males, with a mean age of 34 years (range 16-76 years)] and 0.14 per cent [7 patients, 4 females/3 males, with a mean age of 37.1 years (range 17-62 years)]. The incidence of tooth impaction was 2.94 per cent [147 patients, 101 females/46 males, with a mean age of 29.7 years (range 15-80 years)]. The most frequently impacted tooth was the upper canine followed by the lower canine, lower second premolar, and upper second premolar. No incidence of maxillary first molar impaction was found. Gender and sidedness did not show a statistical significance on the recorded abnormality (P = 0.22 and P = 0.48, respectively). However, in the 15-29 year age group, this was statistically different (P < 0.05). The incidence of transmigration of impacted mandibular canine teeth was greater than other teeth. With the increased use of panoramic radiographs, it is inevitable that diagnosis of such anomalies will increase.

Angular photogrammetric analysis of the soft tissue facial profile of Turkish adults.

One of the most important components of orthodontic diagnosis and treatment planning is the evaluation of the patient's soft tissue profile. The aim of this study was to develop angular photogrammetric standards for Class I Anatolian Turkish males and females. A random sample of 100 Turkish individuals (46 males and 54 females; ages 19-25 years) was obtained. The photographic set-up consisted of a tripod that held a 35 mm camera and a primary flash. The camera was used in its manual position and photographic records were taken of the subjects in natural head posture. The photographic records, 35 mm slide format, were digitized and analyzed using the Quick Ceph Image software program for Windows. Twelve measurements were digitally analyzed on each photograph. For statistical evaluation a Student's t-test was performed and the reliability of the method was analyzed. The results were compared with reported norms of facial aesthetics. The nasofrontal (G-N-Prn), nasal (Cm-Sn/N-Prn), vertical nasal (N-Prn/TV), and nasal dorsum (N-Mn-Prn) angles showed statistically insignificant gender differences (P>0.05). The nasolabial angle (Cm-Sn-Ls) demonstrated large variability. Gender differences were present in the mentolabial (Li-Sm-Pg) and cervicomental (G-Pg/C-Me) angles. The mentolabial angle showed a high method error and large variability. Facial (G-Sn-Pg) and total facial (G-Prn-Pg) convexity angles were similar, while Cm-Sn-Ls angle range was larger compared with other angles. The mean values obtained from this sample can be used for comparison with records of subjects with the same characteristics and following the same photogrammetric technique. Angular photogrammetric profile analysis can provide the orthodontist with a way of determining problems associated with various soft tissue segments of the face.