RESUMO
Glioblastoma (GBM) is the most common glioma in adults, with incidence increasing by 3% per year. According to the World Health Organization Classification of Central Nervous System Tumors, GBM is considered a grade IV tumor due to its malignant behavior. The aim of this review is to summarize the main biological aspects of GBM. In particular, we focused our attention on those alterations which have been proven to have an impact on patients' outcome, mainly in terms of overall survival (OS), or on the tumor response to therapies. We have also analyzed the cellular biology and the interactions between GBM and the surrounding environment.
Assuntos
Biomarcadores Tumorais/genética , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/genética , Gerenciamento Clínico , Glioblastoma/diagnóstico , Glioblastoma/genética , Biomarcadores Tumorais/sangue , Neoplasias Encefálicas/terapia , Receptores ErbB/sangue , Receptores ErbB/genética , Glioblastoma/terapia , Humanos , Proteínas Proto-Oncogênicas B-raf/sangue , Proteínas Proto-Oncogênicas B-raf/genéticaRESUMO
Crystals of human plasma retinol-binding protein have been obtained from 4.5 M-NaCl buffered at pH 6.8 with 20 mM-cacodylate. The crystals are trigonal with space group R3 and unit cell dimensions, referred to the hexagonal system, a = b = 104.2 A and c = 74.5 A. The crystals diffract to a resolution of 2.0 A.
Assuntos
Proteínas de Ligação ao Retinol , Cristalização , Humanos , Proteínas Plasmáticas de Ligação ao Retinol , Difração de Raios XRESUMO
Chicken liver basic fatty acid-binding protein (pI = 9.0) has been purified with a high yield by a modification of a method originally applied to rat liver. The final product is highly homogeneous and can be used to grow crystals that belong to two different space groups. The crystals are either tetragonal, space group P4(2)2(1)2 with a = b = 60.2 A and c = 138.1 A or orthorhombic, space group P2(1)2(1)2(1) with a = 60.7 A, b = 40.1 A and c = 66.7 A. The second form appears to be more suitable for X-ray diffraction studies, it diffracts to at least 2.8 A resolution and it is believed to contain one protein molecule in the crystallographic asymmetric unit.
Assuntos
Proteínas de Transporte/isolamento & purificação , Fígado/análise , Proteínas de Neoplasias , Proteínas do Tecido Nervoso , Aminoácidos/análise , Animais , Galinhas , Cristalografia , Proteína 7 de Ligação a Ácidos Graxos , Proteínas de Ligação a Ácido Graxo , Ponto Isoelétrico , Peso Molecular , Difração de Raios XRESUMO
This is a retrospective study of patients with first attack of myocardial infarction admitted to Medical College Hospital, Calicut during the years 1969 to 1988. The data analysed were compared to other hospital based studies in India and abroad. A striking increase in the percentage of acute myocardial infarction was observed. There was also an increase in the occurrence of myocardial infarction in the young. A properly designed population based study is warranted.
Assuntos
Unidades de Cuidados Coronarianos/estatística & dados numéricos , Hospitais de Ensino/estatística & dados numéricos , Infarto do Miocárdio/epidemiologia , Adulto , Fatores Etários , Idoso , Feminino , Registros Hospitalares/estatística & dados numéricos , Humanos , Incidência , Índia/epidemiologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores Sexuais , Fatores de TempoRESUMO
The three-dimensional structure of chicken liver basic fatty acid-binding protein has been determined at 2.7 A resolution by X-ray crystallography. Phases were calculated using the multiple isomorphous replacement procedure and a preliminary model was built. This model, with an initial R-factor of 0.57, was then improved by a cycle of refinement by simulated annealing which brought the R factor down to 0.32. The protein is structured as a compact 10-stranded-beta-barrel which encapsulates a residual electron density that can be interpreted as a fatty acid molecule. The NH2-terminus portion of the molecule contains two short alpha-helices. The structure of this liver protein appears very similar to that of the Escherichia coli derived rat intestinal FABP recently determined by X-ray diffraction methods.
Assuntos
Proteínas de Transporte/química , Fígado/química , Proteínas de Neoplasias , Proteínas do Tecido Nervoso , Animais , Galinhas , Proteína 7 de Ligação a Ácidos Graxos , Proteínas de Ligação a Ácido Graxo , Ácidos Graxos/metabolismo , Conformação Proteica , Difração de Raios XAssuntos
Absorção Intestinal , Desnutrição Proteico-Calórica/fisiopatologia , Pré-Escolar , Humanos , Índia , LactenteAssuntos
Abscesso Hepático Amebiano , Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Índia , Contagem de Leucócitos , Testes de Função Hepática , Masculino , Pessoa de Meia-IdadeAssuntos
Elastina , Animais , Bovinos , Fenômenos Químicos , Química , Tecido Elástico , Elastina/análise , Raios Infravermelhos , Ligamentos , Lisina , Peptídeos , Soluções , Análise Espectral , Ácidos SulfúricosAssuntos
Peptídeos , Difração de Raios X , Compostos de Benzil , Histidina , Métodos , Conformação MolecularAssuntos
Peptídeos , Fenômenos Químicos , Físico-Química , Dispersão Óptica Rotatória , Polímeros , Triptofano , Difração de Raios XRESUMO
This is a retrospective study of patients with first attack of myocardial infarction admitted to Medical College Hospital, Calicut during the years 1969 to 1988. The data analysed were compared to other hospital based studies in India and abroad. A striking increase in the percentage of acute myocardial infarction was observed. There was also an increase in the occurrence of myocardial infarction in the young. A properly designed population based study is warranted.