[Questionnaire of the Ryzhikh National Medical Research Centre for Coloproctology is a new tool for assessing chronic pelvic pain and pelvic organ dysfunction]. / Oprosnik NMITs koloproktologii imeni A.N. Ryzhikh ­ novyi instrument otsenki khronicheskoi tazovoi boli i narusheniya funktsii tazovykh organov.

The article presents the work of a multidisciplinary team of experts from various fields of medicine to optimize the «Questionnaire for assessing chronic pelvic pain and pelvic organ dysfunction (QCPPD) of the Ryzhikh National Medical Research Centre for Coloproctology¼ for use in clinical practice. The survey of respondents was conducted from June 28 to September 28, 2021. As a result of this survey, by repeatedly making edits and clarifications during communication with respondents, the final version was obtained, which allows assessing the patient's subjective sensations by the nature and localization of pelvic pain, sensitivity disorders and pelvic organ function. The main objective of this Questionnaire is to differentiate patients with neurogenic pain from a huge number of patients with chronic pelvic pain. This aspect will allow a more targeted approach to the diagnosis and pathogenetically justified treatment of patients, including after appropriate instrumental examinations. The work of a multidisciplinary team implies a higher degree of objectification and terminological accuracy of the Questionnaire under discussion. The presented version of the «Questionnaire for assessing chronic pelvic pain and pelvic organ dysfunction (QCPPD) of the Ryzhikh National Medical Research Centre for Coloproctology¼ will be primarily used in coloproctological patients with pelvic pain problems and anal incontinence and obstructive defecation. Further studies will be directed to the clinical evaluation of the results of the work carried out.

Comparison of Fragments in Human Hemostatic Proteins That Mimics Fragments in Proteins of A/H1N1 Viruses and Coronaviruses.

Objective: To compare the repertoire of proteins of the human hemostatic system and fragments mimicking these proteins in the proteins of influenza A/H1N1 viruses and coronaviruses. Material and methods. Influenza viruses A/H1N1 (A/Brevig Mission/1/18), A/St. Petersburg /RII04/2016 (H1N1)pdm09, coronaviruses SARS-CoV and SARS-CoV-2 (strain Wuhan-Hu-1) were used for comparative computer analysis. The sources of the primary structures of proteins of the analyzed viruses and 41 proteins of the human hemostatic system were publicly available Internet databases, respectively, www.ncbi.nlm.nih.gov and www.nextprot.org. The search for homologous sequences in the structure of viral proteins and hemostatic proteins was carried out by comparing fragments of 12 amino acids in length, taking as related those that showed identity at ≥8 positions. Results. Comparative analysis of the repertoire of cellular proteins of the hemostatic system and fragments mimicking these proteins in the structure of proteins of viruses A/H1N1 1918, A(H1N1)pdm09 isolated in 2016, SARS-CoV and SARS-CoV-2, showed a significant difference between SARS-CoV-2 and analyzed viruses. In the protein structure of the SARS-CoV-2 virus, mimicry was revealed for almost all analyzed hemostasis proteins. As for the comparison of viruses A/H1N1 1918, A(H1N1)pdm09 2016 and SARS-CoV, the influenza virus A/H1N1 1918 and SARS-CoV are the closest in the repertoire of hemostatic proteins. Conclusion. Obtained bioinformatic analysis data can serve as a basis for further study of the role of homologous fragments in the regulation of hemostasis of the host organism.

[Modulation of endothelial factors activity in human endothelial cells in influenza A(H1N1)pdm09 virus infection].

INTRODUCTION: Influenza A virus infection can lead to endothelial dysfunction (ED), including apoptosis of endothelial cells and modulation of endothelial factor activities. Affected biochemical factors may include those playing important roles in vascular homeostasis. However, the effect of this pathogen on the expression pattern of key endothelial factors is still unknown.The aim of this work was to study the expression of endothelial nitric oxide synthase (eNOS) and plasminogen activator inhibitor-1 (PAI-1, serpin E1) in the EA.hy926 endothelial cells. RESEARCH OBJECTIVES: to assess expression of eNOS and PAI-1 in endothelial cells infected with influenza virus A(H1N1)pdm09, and to identify homologous fragments in structure of viral proteins and endothelial factors. MATERIAL AND METHODS: Cells were infected with influenza virus A/St. Petersburg/48/16 (H1N1)pdm09 and analyzed in dynamics in 6, 12, 18, 24, 48, and 72 hrs post infection (hpi). Detection of endothelial factors expression levels was performed by immunocytochemical method (ICC) using antibodies for eNOS and PAI-1 while quantitative assessment of expression levels was carried out by program Nis-Elements F3.2 («Nikon¼, Japan). The search for homologous sequences between viral proteins and eNOS and PAI-1 was performed by computer comparison. Sequences were analyzed as fragments 12 amino acid residues (aar) in length. RESULTS AND DISCUSSION: eNOS expression in infected cells had decreased to 7.9% by 6 hpi (control was taken as 100%) to 3.3% at 72 hpi. PAI-1 expression varied significantly over the course of the experiment: by 6 hpi it had decreased to 49.6%, and to 43.2% by 12 hpi. Later PAI-1 levels were: 116.3% (18 hpi); 18.9% (24 hpi); 23.5% (48 hpi), and 35% (72 hpi). CONCLUSION: These results indicate that influenza A infection of endothelial cells causes a significant decrease in eNOS expression, while modulating PAI-1 one. The described phenomenon can be used in the further development of directions of pathogenetic therapy of vascular complications of infection caused by this pathogen.

[Modeling influenza virus infection in mature Wistar rats].

It has now been established that blood vessels are target for influenza, but the mechanism by which the influenza virus affects the cardiovascular system is unknown. The aim - adaptation of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) to mature Wistar rats, as these animals are the main experimental model for studying the pathology of the cardiovascular system. MATERIAL AND METHODS: Passage of influenza A virus (IAV) in embryonated chicken eggs, intranasal inoculation of rats with virus-containing material s, production of pulmonary homogenate, determination of IAV titer in embryonated chicken eggs, detection of histological changes in lung and pulmonary vessels. RESULTS: The article presents the results of the adaptation of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) to mature Wistar rats. The infectious titer of the virus in the homogenates of infected rats lungs at the last stage of adaptation was 7.0 lg EID50/ml. Histological studies revealed pronounced changes in the respiratory tract (spasm of bronchioles, submucosal edema, desquamation of ciliated epithelium of bronchioles) and pulmonary vessels (spasm, desquamation and swelling of endotheliocytes, dissociation and swelling of the elastic membrane and media). In order to identify IAV in blood vessels and lung tissues, an immunohistochemical study was performed using monoclonal antibodies to NP antigen of IAV. CONCLUSION: The data obtained allow us to conclude that the strain of influenza virus A/St. Petersburg/48/16 H1N1(pdm09) was adapted to mature Wistar rats maintaining virulent properties. The infectious titer of the virus at the last stage of adaptation was 7.0 lg EID50/ml. IAV identification is confirmed by immunohistochemical examination.

[Clinical and electrophysiological comparisons in minimal cerebral dysfunction in school-aged children]. / Kliniko-élektrofiziologicheskie sopostavleniia pri minimal'noi tserebral'noi disfunktsii u detei shkol'nogo vozrasta.

Clinico-electrophysiological studies of 74 children of school age with speech disorders demonstrated that the syndrome of the minimal brain dysfunction frequently develops on the background of early organic brain lesions. These data were confirmed by a combination of speech and motor disturbances in 70.3% of the studied children as well as by changes in the electro- and echoencephalograms. That is why one of the criteria for eliminating the syndrome of the minimal brain dysfunction should be the state of the motor and speech systems in combination with EEG data.

[The ratio between the level of the number of sheep botflies and the host immune response]. / Zavisimost' mezhdu urovnem chislennosti ovech'ego ovoda i immunnym otvetom khoziaina.

The dependence of indices of the larvae number and their biomass upon characteristics of the immune response in ELISA at organism and population levels of the Oestris ovis parasitic system is discussed. It was determined, that the majority of larvae distribution excerptions in the host population and the distribution of specific antibodies level indices in the serum blood of animals correspond to the model of the negative binomial distribution. The straight dependence of the parasitic larvae number on the specific antibodies level in the serum blood of host at the organism level of the parasitic system was not determined. The high correlative dependences of the mean number indices and of the distribution of larvae upon the mean value of extinction in the immunity enzyme reaction and upon the mean level of specific antibodies in the serum blood of the host population at the population level were determined. The control of the sheep bot fly larvae number is possible by the determination of the mean specific antibodies level in host population in ELISA.

[Survival of the larvae of the ship botfly Oestrus ovis L. depending on the function of the immune system of the host's body]. / Vyzhivaemost' lichinok ovech'ego ovoda Oestrus ovis L. v zavisimosti ot sostoianiia immunnoi sistemy organizma khoziaina.

In the experiments on artificial infection the survival of larvae of Oestrus ovis L. in sheep with depressed, normal and stimulated immune system was studied. The maximum number of larvae survived in immune depressed animals (62.9%), the minimum number survived in immune stimulated animals (0.4%). For the estimation of specific immune response the reaction of indirect hemagglutination (IHA), the reaction of diffused precipitation (RDP) and the immune ferment analysis (ELISA) were used.

[The T-cell response in parasitization by sheep bot (Oestrus ovis) larvae]. / T-kletochnyi otvet pri parazitirovanii lichinok ovech'ego ovoda Oestrus ovis.

The kinetics of immunocompetent cells in sheep artificially infested with 80, 160, 1000 specimens of Oestrus ovis larvae and immunized with antigen from larvae protein was traced by stableons (E-REC) and antigen-fixing (Ag-REC) rosette formation reaction. It was found that E-REC level changes only a little in both infested and reinfested animals. Ag-REC kinetics mates with the rated larvae biomass changes in all age groups. Ag-REC level of infested animals is much higher and then regularly declines in the following cycles of invasion. T-lymphocytes level and death level of immunized lambs is much higher than in intact animals at the beginning of parasitism.

[The kinetics of specific serum antibodies in sheep infested with sheep bot larvae]. / Kinetika spetsificheskikh syvorotochnykh antitel u ovets, invazirovannykh lichinikami ovech'ego ovoda.

The kinetics of specific antibodies of the blood serum of sheep experimentally infested with 80, 160 and 1000 specimens of Oestrus ovis larvae was examined. The affinity pure serum IgG and the immunoferment analysis (ELISA) were used for qualitative estimation of specific antibodies. It has been shown that the level of specific antibodies correlates with the larval biomass and is connected with ontogenesis of this parasite. The younger animals, which were infested for the first time, are characterized by more intensive production of specific IgG than adult reinfested ones. The ways of immunity response formation in animals infested with Oestrus ovis larvae are considered.

[Clinico-roentgenological characteristics of stomach cancer in young and old patients]. / Kliniko-rentgenologicheskie osobennosti raka zheludka u lits molodogo i pozhilogo vozrasta.

Under study were 3877 case reports of gastric cancer patients of different age groups. It was noted that the duration of complaints in all age groups is nearly the same, but the diagnostic period in younger patients (18-35 years of age), as a rule, is considerably prolonged due to a supposed non-oncological lesion. Also, young patients show a larger area of invasion and earlier metastases. The recognition of gastric ulcer in due course by an aimed roentgenological examination, using also pneumogastrography, is shown to be effective enough.

[Advantages of periodic medical examinations of asbestos plant workers at the Occupational Center]. / Preimushchestva provedeniia periodicheskogo meditsinskogo ostmotra rabochikh asbestovogo zavoda v usloviiakh proftsentra.

The article deals with results of periodic medical examination conducted in Regional Occupational Center MONICI for 90 subjects working in apparatus workshop of asbestos ware plant. Data presented are results of thorough evaluation of respiration biomechanics through electronic spirometry and body plethysmography, serum activity of interstitial alveolomucin 3EG5 and oncologic markers CA-125 and CA-15.3.

[X-ray densitometry and biochemical parameters of bone tissue metabolism in patients with vibration disease]. / Rentgenodensitometricheskie i biokhimicheskie pokazateli metabolizma kostnoi tkani u bol'nykh vibratsionnoi bolezn'iu.

To reveal systemic and local osteoporosis, the authors studied biochemical markers of bone metabolism in vibration disease patients. The vibration disease patients appeared to have the most frequent and marked osteoporosis in peripheral bones--hands (in 90% of cases) and forearms (in 66.7%). Prevalence of systemic osteoporosis and osteopenia reached 11.7 and 48.3% in the select respectively. According to biochemical markers, bone reconstruction state was characterized by moderately intensified bone resorption and diminished bone formation.

[Prevention of respiratory infections by physical methods in children in tuberculosis sanatoria]. / Profilaktika respiratornoi infektsii fizicheskimi metodami u detei v tuberkuleznykh sanatoriiakh.

Follow-up of over 12 years in a children's sanatorium included 6035 tuberculosis children aged 3-14 years who had tuberculin tests reversion and local forms of tuberculosis. Prevention by physical methods (herb infusion inhalations, ultraviolet radiation and nasopharyngeal rinsing) caused an average decrease in the incidence of ARD from 22.66 to 5.58%. The efficiency of sanatorium treatment also increased.