RESUMO
A Klebsiella pneumoniae culture in Fouad's chemically defined medium, which was grown from 18 to 24 hours at 37 degrees C was prepared. Bacterial cells were eliminated by centrifugation and the supernatant was sterilized by Millipore membrane filtration. A protective activity against normal human serum bactericidal effect on K. pneumoniae K-9 and K-13 was ascribed to sterile filtrate. The protective filtrate seems to be a high molecular weight substance.
Assuntos
Fatores Biológicos/isolamento & purificação , Atividade Bactericida do Sangue/efeitos dos fármacos , Klebsiella pneumoniae/metabolismo , Fatores Biológicos/farmacologia , Meios de Cultivo Condicionados/química , Diálise , Humanos , Klebsiella pneumoniae/crescimento & desenvolvimento , UltrafiltraçãoRESUMO
Chemical composition of a substance produced by Klebsiella pneumoniae which is accumulated in chemically defined medium was determined. This substance (the protective factor) protects the bacterium from being killed by normal human serum. Protective factor was treated with DNase, lysozyme and an alkaline protease. The two former enzymes did not affect the protective factor. On the other hand, when alkaline protease was used, the protective activity was totally lost. Results showed that the protective factor is a protein.
Assuntos
Proteínas de Bactérias/isolamento & purificação , Fatores Biológicos/isolamento & purificação , Atividade Bactericida do Sangue/efeitos dos fármacos , Klebsiella pneumoniae/fisiologia , Proteínas de Bactérias/farmacologia , Fatores Biológicos/farmacologia , Cromatografia em Agarose , Meios de Cultivo Condicionados/química , Desoxirribonucleases/metabolismo , Endopeptidases/metabolismo , Humanos , Klebsiella pneumoniae/química , Muramidase/metabolismoRESUMO
It has been considered that the polysaccharide capsular material from microorganisms such as Klebsiella pneumoniae induces mainly thymus-independent humoral immunity, nevertheless studies done with Bacteroides fragilis have shown the participation of cellular immune effector mechanisms. Works done to define the immunogenic role of the capsular polysaccharide of Klebsiella pneumoniae indicate that both active and passive immunization with these antigens confer protection against infections with this bacteria. Several studies propose the use of vaccines prepared with one or various capsular serotypes of Klebsiella pneumoniae in immunoprophylaxis or immunotherapy.
Assuntos
Cápsulas Bacterianas/imunologia , Klebsiella pneumoniae , Animais , Formação de Anticorpos , Vacinas Bacterianas , Humanos , Imunidade Celular , Imunização , Infecções por Klebsiella/prevenção & controleRESUMO
Fourteen different plant seeds were used to obtain lectins which in turn were used to agglutinate 72 different serological strains of Klebsiella. The results were used to design a scheme which distinguishes 62 serotypes (91.6%) with a unique agglutination pattern with lectins. Two pairs of strains as well as two sets of three strains gave the same patterns. This procedure is useful as an alternative in the identification of strains for epidemiological purposes.
Assuntos
Klebsiella/classificação , Testes de Aglutinação , Humanos , LectinasRESUMO
Presentamos un recién nacido varón de 38 semanasde edad gestacional que presenta al nacimientolesiones ulcerosas en cuero cabelludo, tronco y extremidadescon limitación en la extensión de codosy rodillas. Fue un embarazo gemelar y el primer gemelofalleció intraútero a las 14 semanas de gestación.La madre no estuvo expuesta a tóxicos ni a radiaciones.Estuvo ingresado 10 días en Neonatología,recibió tratamiento antibiótico tópico y fue dadode alta con clara mejoría de las lesiones así comode la limitación articular dicha
We present a male newborn of 38 weeks of gestationalage that presents on being born ulcerous injuriesin scalp, trunk and limbs with limitation in the extensionof elbows and knees. He was a twin pregnancyand the first twin died intrauterine to the 14weeks of gestation. The mother was exposed neithertoxics nor radiations. The boy was hospitalized10 days in Neonatology, he received antibiotic topicaltreatment and he was discharged with clear improvementof the injuries and of the articulated limitationreported
Assuntos
Humanos , Masculino , Recém-Nascido , Displasia Ectodérmica/diagnóstico , Telangiectasia/congênito , Hemangioma/congênito , Diagnóstico DiferencialRESUMO
Se aisló un bacteriólogo de K. pneumoniae K-8 el cual induce la síntesis de una enzima que despolimeriza el polisacárido capsular de la cepa huésped. la despolimerasa fue purificada y se determinaron algunas condiciones que influyen en su actividad, como son el tiempo de incubación, las concentraciones de enzima y substrato, el pH, la temperatura de incubación y la constante de Michaelis (Km). Se encontró que la enzima hidroliza parcialmente el polisacárideo. No se detectaron azúcares reductores como productos de la hidrólisis enzimática