RESUMO
Piezo channels are a ubiquitously expressed, principal type of molecular force sensor in eukaryotes. They enable cells to decode a myriad of physical stimuli and are essential components of numerous mechanosensory processes. Central to their physiological role is the ability to change conformation in response to mechanical force. Here we discuss the evolutionary origin of Piezo in relation to other MS channels in addition to the force that gates Piezo channels. In particular, we discuss whether Piezo channels are inherently mechanosensitive in accordance with the force-from-lipid paradigm which has been firmly established for bacterial MS channels and two-pore domain K+ (K2P) channels. We also discuss the evidence supporting a reliance on or direct interaction with structural scaffold proteins of the cytoskeleton and extracellular matrix according to the force-from-filament principle. In doing so, we explain the false dichotomy that these distinctions represent. We also discuss the possible unifying models that shed light on channel mechanosensitivity at the molecular level.
Assuntos
Canais Iônicos/metabolismo , Metabolismo dos Lipídeos , Mecanotransdução Celular , Animais , HumanosRESUMO
Biological membranes are essential for normal function and regulation of cells, forming a physical barrier between extracellular and intracellular space and cellular compartments. These physical barriers are subject to mechanical stresses. As a consequence, nature has developed proteins that are able to transpose mechanical stimuli into meaningful intracellular signals. These proteins, termed Mechanosensitive (MS) proteins provide a variety of roles in response to these stimuli. In prokaryotes these proteins form transmembrane spanning channels that function as osmotically activated nanovalves to prevent cell lysis by hypoosmotic shock. In eukaryotes, the function of MS proteins is more diverse and includes physiological processes such as touch, pain and hearing. The transmembrane portion of these channels is influenced by the physical properties such as charge, shape, thickness and stiffness of the lipid bilayer surrounding it, as well as the bilayer pressure profile. In this review we provide an overview of the progress to date on advances in our understanding of the intimate biophysical and chemical interactions between the lipid bilayer and mechanosensitive membrane channels, focusing on current progress in both eukaryotic and prokaryotic systems. These advances are of importance due to the increasing evidence of the role the MS channels play in disease, such as xerocytosis, muscular dystrophy and cardiac hypertrophy. Moreover, insights gained from lipid-protein interactions of MS channels are likely relevant not only to this class of membrane proteins, but other bilayer embedded proteins as well. This article is part of a Special Issue entitled: Lipid-protein interactions.
Assuntos
Membrana Celular/metabolismo , Mecanotransdução Celular , Lipídeos de Membrana/metabolismo , Proteínas de Membrana/metabolismo , Animais , Membrana Celular/química , Humanos , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Lipídeos de Membrana/química , Proteínas de Membrana/química , Modelos Moleculares , Ligação Proteica , Estrutura Terciária de ProteínaRESUMO
The mechanosensitive channel of small conductance (MscS)-like channel superfamily is present in cell-walled organisms throughout all domains of life (Bacteria, Archaea and Eukarya). In bacteria, members of this channel family play an integral role in the protection of cells against acute downward shifts in environmental osmolarity. In this review, we discuss how evolutionary 'tinkering' has taken MscS-like channels from their currently accepted physiological function in bacterial osmoregulation to potential roles in processes as diverse as amino acid efflux, Ca(2+) regulation and cell division. We also illustrate how this structurally and functionally diverse family of channels represents an essential industrial component in the production of monosodium glutamate, an attractive antibiotic target and a rich source of membrane proteins for the studies of molecular evolution.
Assuntos
Bactérias/genética , Membrana Celular/genética , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/genética , Evolução Molecular , Canais Iônicos/química , Canais Iônicos/genética , Mecanotransdução Celular/genética , Sequência de Aminoácidos , Bactérias/química , Sequência de Bases , Membrana Celular/química , Membrana Celular/ultraestrutura , Proteínas de Escherichia coli/ultraestrutura , Variação Genética/genética , Ativação do Canal Iônico/genética , Canais Iônicos/ultraestrutura , Fluidez de Membrana/genética , Dados de Sequência Molecular , Estresse Mecânico , Relação Estrutura-AtividadeRESUMO
Mechanosensitive (MS) ion channels are primary transducers of mechanical force into electrical and/or chemical intracellular signals. Many diverse MS channel families have been shown to respond to membrane forces. As a result of this intimate relationship with the membrane and proximal lipids, amphipathic compounds exert significant effects on the gating of MS channels. Here, we performed all-atom molecular dynamics (MD) simulations and employed patch-clamp recording to investigate the effect of two amphipaths, Fluorouracil (5-FU) a chemotherapy agent, and the anaesthetic trifluoroethanol (TFE) on structurally distinct mechanosensitive channels. We show that these amphipaths have a profound effect on the bilayer order parameter as well as transbilayer pressure profile. We used bacterial mechanosensitive channels (MscL/MscS) and a eukaryotic mechanosensitive channel (TREK-1) as force-from-lipids reporters and showed that these amphipaths have differential effects on these channels depending on the amphipaths' size and shape as well as which leaflet of the bilayer they incorporate into. 5-FU is more asymmetric in shape and size than TFE and does not penetrate as deep within the bilayer as TFE. Thereby, 5-FU has a more profound effect on the bilayer and channel activity than TFE at much lower concentrations. We postulate that asymmetric effects of amphipathic molecules on mechanosensitive membrane proteins through the bilayer represents a general regulatory mechanism for these proteins.
Assuntos
Proteínas de Escherichia coli , Humanos , Escherichia coli/metabolismo , Proteínas de Escherichia coli/metabolismo , Fluoruracila/farmacologia , Canais Iônicos/metabolismo , Bicamadas Lipídicas/química , Lipídeos/farmacologia , Mecanotransdução Celular , Trifluoretanol/metabolismoRESUMO
In this review, we present our current understanding of peripartum cardiomyopathy (PPCM) based on reports of the incidence, diagnosis and current treatment options. We summarise opinions on whether PPCM is triggered by vascular and/or hormonal causes and examine the influence of comorbidities such as preeclampsia. Two articles published in 2021 strongly support the hypothesis that PPCM may be a familial disease. Using large cohorts of PPCM patients, they summarised the available genomic DNA sequence data that are expressed in human cardiomyocytes. While PPCM is considered a disease predominately affecting the left ventricle, there are data to suggest that some cases also involve right ventricular failure. Finally, we conclude that there is sufficient evidence to warrant an RNAseq investigation and that this would be most informative if performed at the cardiomyocytes level rather than analysing genomic DNA from the peripheral circulation. Given the rarity of PPCM, the combined resources of international human heart tissue biobanks have assembled 30 ventricular tissue samples from PPCM patients, and we are actively seeking to enlarge this patient base by collaborating with human heart tissue banks and research laboratories who would like to join this endeavour.
RESUMO
Mechanosensitive ion channels use mechanical energy to gate the dissipation of electrochemical gradients across cell membranes. This function is fundamental to physiological processes such as hearing and touch. In electrophysiological studies of ion channels in the plasma membrane of the yeast Saccharomyces cerevisiae, channels were observed that were activated by, and adapted to, stretching of the membrane. Adaptation of channel activity to mechanical stimuli was voltage-dependent. Because these mechanosensitive channels pass both cations and anions, they may play a role in turgor regulation in this walled organism.
Assuntos
Membrana Celular/fisiologia , Canais Iônicos/fisiologia , Saccharomyces cerevisiae/fisiologia , Adaptação Fisiológica , Ânions/fisiologia , Cátions/fisiologia , Permeabilidade da Membrana Celular , Pressão Hidrostática , Potenciais da Membrana , Tensão Superficial , Equilíbrio HidroeletrolíticoRESUMO
Voltage-dependent ion channels have been found in the plasma membrane of the yeast Saccharomyces cerevisiae. Ion channel activities were recorded from spheroplasts or patches of plasma membrane with the patch-clamp technique. The most prominent activities came from a set of potassium channels with the properties of activation by positive but not negative voltages, high selectivity for potassium over sodium ion, unit conductance of 20 picosiemens, inhibition by tetraethylammonium or barium ions, and bursting kinetics.
Assuntos
Canais Iônicos/fisiologia , Saccharomyces cerevisiae/fisiologia , Membrana Celular/fisiologia , Eletrofisiologia , Potenciais da Membrana , Potássio/metabolismo , Sódio/metabolismoRESUMO
OBJECTIVE: To investigate the effect of glucosamine on the loss of newly synthesized radiolabeled large and small proteoglycans by bovine tendon, ligament and joint capsule. DESIGN: The kinetics of loss of (35)S-labeled large and small proteoglycans from explant cultures of tendon, ligament and joint capsule treated with 10mM glucosamine was investigated over a 10-day culture period. The kinetics of loss of (35)S-labeled small proteoglycans and the formation of free [(35)S]sulfate were determined for the last 10 days of a 15-day culture period. The proteoglycan core proteins were analyzed by gel electrophoresis followed by fluorography. The metabolism of tendon, ligament and joint capsule explants exposed to 10mM glucosamine was evaluated by incorporation of [(3)H]serine and [(35)S]sulfate into protein and glycosaminoglycans, respectively. RESULTS: Glucosamine at 10mM stimulated the loss of small proteoglycans from ligament explant cultures. This was due to the increased loss of both macromolecular and free [(35)S]sulfate to the medium indicating that glucosamine affected the release of small proteoglycans as well as their intracellular degradation. The degradation pattern of small proteoglycans in ligament was not affected by glucosamine. In contrast, glucosamine did not have an effect on the loss of large or small proteoglycans from tendon and joint capsule or large proteoglycans from ligament explant cultures. The metabolism of cells in tendon, ligament and joint capsule was not impaired by the presence of 10mM glucosamine. CONCLUSIONS: Glucosamine stimulated the loss of small proteoglycans from ligament but did not have an effect on small proteoglycan catabolism in joint capsule and tendon or large proteoglycan catabolism in ligament, tendon or synovial capsule. The consequences of glucosamine therapy at clinically relevant concentrations on proteoglycan catabolism in joint fibrous connective tissues need to be further assessed in an animal model.
Assuntos
Glucosamina/farmacologia , Cápsula Articular/metabolismo , Ligamentos Articulares/metabolismo , Proteoglicanas/efeitos dos fármacos , Tendões/metabolismo , Animais , Bovinos , Células Cultivadas , Relação Dose-Resposta a Droga , Regulação para CimaRESUMO
Gating of mechanosensitive (MS) channels is driven by a hierarchical cascade of movements and deformations of transmembrane helices in response to bilayer tension. Determining the intrinsic mechanical properties of the individual transmembrane helices is therefore central to understanding the intricacies of the gating mechanism of MS channels. We used a constant-force steered molecular dynamics (SMD) approach to perform unidirectional pulling tests on all the helices of MscL in M. tuberculosis and E. coli homologs. Using this method, we could overcome the issues encountered with the commonly used constant-velocity SMD simulations, such as low mechanical stability of the helix during stretching and high dependency of the elastic properties on the pulling rate. We estimated Young's moduli of the α-helices of MscL to vary between 0.2 and 12.5 GPa with TM2 helix being the stiffest. We also studied the effect of water on the properties of the pore-lining TM1 helix. In the absence of water, this helix exhibited a much stiffer response. By monitoring the number of hydrogen bonds, it appears that water acts like a 'lubricant' (softener) during TM1 helix elongation. These data shed light on another physical aspect underlying hydrophobic gating of MS channels, in particular MscL.
Assuntos
Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/metabolismo , Canais Iônicos/química , Canais Iônicos/metabolismo , Fenômenos Mecânicos , Simulação de Dinâmica Molecular , Nanotecnologia , Fenômenos Biomecânicos , Elasticidade , Porosidade , Conformação Proteica em alfa-HéliceRESUMO
Hollow organs (e.g. heart) experience pressure-induced mechanical wall stress sensed by molecular mechano-biosensors, including mechanosensitive ion channels, to translate into intracellular signaling. For direct mechanistic studies, stretch devices to apply defined extensions to cells adhered to elastomeric membranes have stimulated mechanotransduction research. However, most engineered systems only exploit unilateral cellular stretch. In addition, it is often taken for granted that stretch applied by hardware translates 1:1 to the cell membrane. However, the latter crucially depends on the tightness of the cell-substrate junction by focal adhesion complexes and is often not calibrated for. In the heart, (increased) hemodynamic volume/pressure load is associated with (increased) multiaxial wall tension, stretching individual cardiomyocytes in multiple directions. To adequately study cellular models of chronic organ distension on a cellular level, biomedical engineering faces challenges to implement multiaxial cell stretch systems that allow observing cell reactions to stretch during live-cell imaging, and to calibrate for hardware-to-cell membrane stretch translation. Here, we review mechanotransduction, cell stretch technologies from uni-to multiaxial designs in cardio-vascular research, and the importance of the stretch substrate-cell membrane junction. We also present new results using our IsoStretcher to demonstrate mechanosensitivity of Piezo1 in HEK293 cells and stretch-induced Ca2+ entry in 3D-hydrogel-embedded cardiomyocytes.
Assuntos
Engenharia Biomédica/métodos , Sistema Cardiovascular/citologia , Fenômenos Mecânicos , Mecanotransdução Celular , Animais , Fenômenos Biomecânicos , Técnicas Biossensoriais , HumanosRESUMO
Mechanosensation in many organs (e.g. lungs, heart, gut) is mediated by biosensors (like mechanosensitive ion channels), which convert mechanical stimuli into electrical and/or biochemical signals. To study those pathways, technical devices are needed that apply strain profiles to cells, and ideally allow simultaneous live-cell microscopy analysis. Strain profiles in organs can be complex and multiaxial, e.g. in hollow organs. Most devices in mechanobiology apply longitudinal uniaxial stretch to adhered cells using elastomeric membranes to study mechanical biosensors. Recent approaches in biomedical engineering have employed intelligent systems to apply biaxial or multiaxial stretch to cells. Here, we present an isotropic cell stretch system (IsoStretcher) that overcomes some previous limitations. Our system uses a rotational swivel mechanism that translates into a radial displacement of hooks attached to small circular silicone membranes. Isotropicity and focus stability are demonstrated with fluorescent beads, and transmission efficiency of elastomer membrane stretch to cellular area change in HeLa/HEK cells. Applying our system to lamin-A overexpressing fibrosarcoma cells, we found a markedly reduced stretch of cell area, indicative of a stiffer cytoskeleton. We also investigated stretch-activated Ca(2+) entry into atrial HL-1 myocytes. 10% isotropic stretch induced robust oscillating increases in intracellular Fluo-4 Ca(2+) fluorescence. Store-operated Ca(2+) entry was not detected in these cells. The Isostretcher provides a useful versatile tool for mechanobiology.
Assuntos
Técnicas Biossensoriais/instrumentação , Dimetilpolisiloxanos/química , Mecanotransdução Celular , Membranas Artificiais , Estresse Mecânico , Cálcio/metabolismo , Linhagem Celular , Linhagem Celular Tumoral , Células Cultivadas , Desenho de Equipamento , Fibrossarcoma/patologia , Células HEK293 , Células HeLa , Humanos , Miócitos Cardíacos/metabolismoRESUMO
Transmembrane calcium fluxes related to excitation were studied in Paramecium caudatum. Radioactive (45Ca2+) or inactive solution was flowed through a dense suspension of unlabelled or labelled cells, and radioactivity was monitored in the solution. The organisms were electrically stimulated by means of extracellular electrodes. As a result of stimulation Ca2+ uptake and release was measured. The uptake response dropped with increasing number of successive stimulation periods and increased with growing stimulus amplitude and duration. Maximum uptake was obtained at 20 V/cm and at least 60 s duration and for temperatures between 10 and 15 degrees C. A Ca2+ influx of 700 pmol/1000 cells upon 1 min stimulation was measured at 15 degrees C. This corresponds to an increment of the intraciliary [Ca2+] of about 5 x 10(-4) M. Ca2+ release was dependent on the stimulus amplitude in a similar manner as was Ca2+ uptake. Photographic recordings of the swimming behaviour of the organisms were used to interpret the flux data. At temperatures up to 15 degrees C the cells swam backward perpendicular to the applied electric field of 10 to 20 V/cm. At 25 degrees C they showed forward spiralling movement. For the first time evidence was brought for stimulated Ca2+ influx in Paramecium at physiological temperatures. It is concluded from the results that a strong active Ca2+ extrusion from the intraciliary space counteracts the influx. The Ca2+ pump rate must be at least 8 x 10(12) calcium ions per s per cm2 ciliary surface.
Assuntos
Cálcio/metabolismo , Membrana Celular/fisiologia , Paramecium/metabolismo , Animais , Transporte Biológico Ativo , Movimento Celular , Estimulação Elétrica , Cinética , Potenciais da Membrana , TemperaturaRESUMO
The electrical properties of Escherichia coli cells were examined by the patch-clamp technique. Giant cells or giant spheroplasts were generated by five different methods. By electron micrographic and other criteria we determined that the patches are most likely from the outer membrane. We regularly observed currents through at least two types of channels in this membrane. The first current is mechanosensitive and voltage-dependent, and can be observed in single gene mutants of the known major porins (ompF, ompC, phoE, lamB); this channel may represent a minor porin or a new class of outer membrane protein. The possible identity of the second, voltage-sensitive channel with one of the known outer membrane proteins is being explored. The high-resistance seals consistently formed on these patches and the presence of gated ion channels suggest that most of the pores of the outer membrane are not statically open, as commonly held, but are closed at rest and may be openable by physiological stimuli.
Assuntos
Escherichia coli/fisiologia , Canais Iônicos/fisiologia , Proteínas da Membrana Bacteriana Externa , Membrana Celular/fisiologia , Escherichia coli/ultraestrutura , Mecanorreceptores/fisiologia , Potenciais da Membrana , Microscopia Eletrônica , Muramidase/farmacologia , PorinasRESUMO
The mechanosensitive channel from Escherichia coli (Eco-MscL) responds to membrane lateral tension by opening a large, water-filled pore that serves as an osmotic safety valve. In an attempt to understand the structural dynamics of MscL in the closed state and under physiological conditions, we have performed a systematic site-directed spin labeling study of this channel reconstituted in a membrane bilayer. Structural information was derived from an analysis of probe mobility, residue accessibility to O(2) or NiEdda and overall intersubunit proximity. For the majority of the residues studied, mobility and accessibility data showed a remarkable agreement with the Mycobacterium tuberculosis crystal structure, clearly identifying residues facing the large water-filled vestibule at the extracellular face of the molecule, the narrowest point along the permeation pathway (residues 21-26 of Eco-MscL), and the lipid-exposed residues in the peripheral transmembrane segments (TM2). Overall, the present dataset demonstrates that the transmembrane regions of the MscL crystal structure (obtained in detergent and at low pH) are, in general, an accurate representation of its structure in a membrane bilayer under physiological conditions. However, significant differences between the EPR data and the crystal structure were found toward the COOH-terminal end of TM2.
Assuntos
Ácido Edético/análogos & derivados , Proteínas de Escherichia coli , Canais Iônicos/química , Canais Iônicos/genética , Sequência de Aminoácidos/genética , Cristalografia , Cisteína/genética , Ácido Edético/metabolismo , Espectroscopia de Ressonância de Spin Eletrônica , Canais Iônicos/metabolismo , Lipossomos , Mecanorreceptores/fisiologia , Conformação Molecular , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Mutação/genética , Oxigênio/metabolismo , Marcadores de Spin , Estresse MecânicoRESUMO
Mechanosensation in bacteria involves transducing membrane stress into an electrochemical response. In Escherichia coli and other bacteria, this function is carried out by a number of proteins including MscL, the mechanosensitive channel of large conductance. MscL is the best characterized of all mechanosensitive channels. It has been the subject of numerous structural and functional investigations. The explosion in experimental data on MscL recently culminated in the solution of the three-dimensional structure of the MscL homologue from Mycobacterium tuberculosis. In this review, much of these data are united and interpreted in terms of the newly published M. tuberculosis MscL crystal structure.
Assuntos
Proteínas de Bactérias/química , Proteínas de Escherichia coli , Canais Iônicos/química , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Ativação do Canal Iônico , Canais Iônicos/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Mycobacterium tuberculosis/química , Conformação Proteica , Homologia de Sequência de AminoácidosRESUMO
The outer membrane of Escherichia coli is a diffusion barrier for macromolecules, but allows the passage of small hydrophilic solutes through non-specific channels, the porins. Some electrophysiological studies find reconstituted porins in a mostly open state, while those done with the patch-clamp technique performed on live cells suggest that the vast majority of the native channels are closed. We present here current measurements through porins from reconstituted outer membrane, which demonstrate that bacterial metabolites, the MDO's, which bathe the periplasmic side of the outer membrane, induce the channels to close. These findings illustrate that the degree of openness of porins can be regulated by compounds naturally found in bacteria.
Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Escherichia coli/metabolismo , Canais Iônicos/metabolismo , Oligossacarídeos/farmacologia , Relação Dose-Resposta a Droga , Canais Iônicos/efeitos dos fármacos , Potenciais da Membrana , Microeletrodos , PorinasRESUMO
By using a functional approach of reconstituting detergent-solubilized membrane proteins into liposomes and following their function in patch-clamp experiments, we identified a novel mechanosensitive (MS) channel in the thermophilic cell wall-less archaeon Thermoplasma volcanium. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the enriched protein fractions revealed a band of approx 15 kDa comparable to MscL, the bacterial MS channel of large conductance. 20 N-terminal residues determined by protein microsequencing, matched the sequence to an unknown open reading frame in the genome of a related species Thermoplasma acidophilum. The protein encoded by the T. acidophilum gene was cloned and expressed in Escherichia coli and reconstituted into liposomes. When examined for function, the reconstituted protein exhibited properties typical of an MS ion channel: 1) activation by negative pressure applied to the patch-clamp pipet, 2) blockage by gadolinium, and 3) activation by the anionic amphipath trinitrophenol. In analogy to the nomenclature used for bacterial MS channels, the MS channel of T acidophilum was termed MscTA. Secondary structural analysis indicated that similar to MscL, the T. acidophilum MS protein may have two transmembrane domains, suggesting that MS channels of thermophilic Archaea belong to a family of structurally related MscL-like ion channels with two membrane-spanning regions. When the mscTA gene was expressed in the mscL- knockout strain and the MscTA protein reconstituted into liposomes, the gating of MscTA was characterized by very brief openings of variable conductance. In contrast, when the mscTA gene was expressed in the wild-type mscL+ strain of E. coli, the gating properties of the channel resembled MscL. However, the channel had reduced conductance and differed from MscL in its kinetics and in the free energy of activation, suggesting that MscTA and MscL can form functional complexes and/or modulate each other activity. Similar to MscL, MscTA exhibited an increase in activity in liposomes made of phospholipids having shorter acyl chain, suggesting a role of hydrophobic mismatch in the function of prokaryotic MS channels.
Assuntos
Parede Celular/fisiologia , Proteínas de Escherichia coli , Canais Iônicos/química , Canais Iônicos/genética , Thermoplasma/metabolismo , Thermoplasma/fisiologia , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Western Blotting , Membrana Celular/metabolismo , Sistema Livre de Células , Clonagem Molecular , Cães , Eletroforese em Gel de Poliacrilamida , Eletrofisiologia , Escherichia coli/metabolismo , Gadolínio/farmacologia , Canais Iônicos/metabolismo , Lipossomos/metabolismo , Dados de Sequência Molecular , Pressão , Ligação Proteica , Estrutura Secundária de Proteína , Coelhos , Homologia de Sequência de Aminoácidos , Fatores de TempoRESUMO
The ubiquity of mechanosensitive (MS) channels triggered a search for their functional homologues in Archaea, the third domain of the phylogenetic tree. Two types of MS channels have been identified in the cell membranes of Haloferax volcanii using the patch clamp technique. Recently MS channels were identified and cloned from two archaeal species occupying different environmental habitats. These studies demonstrate that archaeal MS channels share structural and functional homology with bacterial MS channels. The mechanical force transmitted via the lipid bilayer alone activates all to date known prokaryotic MS channels. This implies the existence of a common gating mechanism for bacterial as well as archaeal MS channels according to the bilayer model. Based on recent evidence that the bilayer model also applies to eukaryotic MS channels, mechanosensory transduction probably originated along with the appearance of the first life forms according to simple biophysical principles. In support of this hypothesis the phylogenetic analysis revealed that prokaryotic MS channels of large and small conductance originated from a common ancestral molecule resembling the bacterial MscL channel protein. Furthemore, bacterial and archaeal MS channels share common structural motifs with eukaryotic channels of diverse function indicating the importance of identified structures to the gating mechanism of this family of channels. The comparative approach used throughout this review should contribute towards understanding of the evolution and molecular basis of mechanosensory transduction in general.
Assuntos
Archaea/metabolismo , Canais Iônicos/metabolismo , Motivos de Aminoácidos , Sequência de Aminoácidos , Evolução Biológica , Membrana Celular/metabolismo , Parede Celular/metabolismo , Clonagem Molecular , Proteínas de Escherichia coli/metabolismo , Ésteres/metabolismo , Éteres/metabolismo , Concentração de Íons de Hidrogênio , Bicamadas Lipídicas , Lipídeos/química , Mathanococcus/metabolismo , Dados de Sequência Molecular , Técnicas de Patch-Clamp , Peptidoglicano/química , Filogenia , Pressão , Estrutura Secundária de Proteína , Homologia de Sequência de Aminoácidos , Transdução de Sinais , Fatores de TempoRESUMO
The reconstitution of bacterial porins in liposome bilayers for patch-clamp recording is well established. However, the solutions used in the dehydration, rehydration, and osmotic swelling of the liposomes have been developed for porins from enteric bacteria. Porins from marine bacteria normally function in contact with seawater whose ionic composition and osmotic pressure would appear to be incompatible with the established methods. Here, we show that, contrary to expectation, an established reconstitution and patch-clamp method works well with porins, mainly OmpH and OmpL, extracted from the deep-sea marine bacterium Photobacterium profundum strain SS9 and that seawater can be introduced at a supplementary stage.
Assuntos
Proteínas de Bactérias , Proteínas de Escherichia coli , Lipossomos/metabolismo , Técnicas de Patch-Clamp/métodos , Photobacterium/metabolismo , Porinas/química , Proteínas da Membrana Bacteriana Externa/metabolismo , Cadaverina/metabolismo , Escherichia coli/metabolismo , Concentração de Íons de Hidrogênio , Porinas/metabolismo , Pressão , Temperatura , Fatores de TempoRESUMO
Porin OmpC from Escherichia coli was reconstituted in liposomes and its gating kinetics were recorded at high hydrostatic pressure, up to 90 MPa, using a development of the patch clamp technique. The composition of the recording solution influenced the results but generally high hydrostatic pressure favoured channel opening.