Circovirus in tissues of dogs with vasculitis and hemorrhage.

We characterized the complete genome of a novel dog circovirus (DogCV) from the liver of a dog with severe hemorrhagic gastroenteritis, vasculitis, and granulomatous lymphadenitis. DogCV was detected by PCR in fecal samples from 19/168 (11.3%) dogs with diarrhea and 14/204 (6.9%) healthy dogs and in blood from 19/409 (3.3%) of dogs with thrombocytopenia and neutropenia, fever of unknown origin, or past tick bite. Co-infection with other canine pathogens was detected for 13/19 (68%) DogCV-positive dogs with diarrhea. DogCV capsid proteins from different dogs varied by up to 8%. In situ hybridization and transmission electron microscopy detected DogCV in the lymph nodes and spleens of 4 dogs with vascular compromise and histiocytic inflammation. The detection of a circovirus in tissues of dogs expands the known tropism of these viruses to a second mammalian host. Our results indicate that circovirus, alone or in co-infection with other pathogens, might contribute to illness and death in dogs.

Divergent astrovirus associated with neurologic disease in cattle.

Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for viral RNA. Viral RNA was restricted to the nervous system and detected in the cytoplasm of affected neurons within the spinal cord, brainstem, and cerebellum. Microscopically, the lesions were of widespread neuronal necrosis, microgliosis, and perivascular cuffing preferentially distributed in gray matter and most severe in the cerebellum and brainstem, with increasing intensity caudally down the spinal cord. These results suggest that infection with BoAstV-NeuroS1 is a potential cause of neurologic disease in cattle.

Isospora troglodytes n. sp. (Apicomplexa: Eimeriidae), a new coccidian species from wrens of Costa Rica.

Nineteen (91%) of 21 rufous-and-white wrens (Thryothorus rufalbus) and five (71%) of seven plain wrens (Cantorchilus modestus) sampled from Costa Rica were positive for a new species of Isospora. Oocysts have a thin, smooth, double, colorless wall and measure 20.1 ± 1.4 × 23.4 ± 1.5 µm (18-24 × 20-26 µm) with an average length-width ratio of 1.2 µm. Sporocysts are ovoid, measure 9.5 ± 0.9 × 15.5 ± 1.1 µm (7-12 × 12-18 µm) with an average length-width ratio of 1.6 µm. A nipple-like steida body continuous with the sporocyst wall and a prominent oval-shaped substeida body are present. In addition to the four sporozoites, a single compact sporocyst residuum was present in each sporocyst. This is the first description of an Isospora species from the family Troglodytidae and the first report of Isospora from the rufous-and-white wren and plain wren.

Cutaneous Filariasis in Free-Ranging Rothschild's Giraffes (Giraffa camelopardalis rothschildi) in Uganda.

Across Africa, wild giraffes suffer from a variety of skin disorders of mostly unknown etiology. With their populations already threatened from anthropogenic factors, it is important to understand infectious disease risks to giraffes. Here we describe filarid parasites and a portion of their genetic sequence associated with skin disease in Rothschild's giraffes (Giraffa camelopardalis rothschildi) in Uganda.

Operation Canine Lifeline: Recommendations for Enhancing Prehospital Care for Government Working Dogs.

Operation Canine Lifeline was a tabletop exercise developed by students and faculty of Boston University School of Medicine's Healthcare Emergency Management master's program. The tabletop exercise led to discussion on current protocols for canines working in the field, what occurs if a canine encounters a toxin in the field, and what to do in situations of national security that require working with civilian agencies. This discussion led to the creation of a set of recommendations around providing prehospital veterinary care to government working dogs. The recommendations include a government-run veterinary toxicology hotline for the sole use of the government, issuing handlers deployment kits and preprogrammed smartphones that contain information on the care practices for dogs, and an increased effort for civilian integration, through local emergency medical services, in the emergency care of government canines. (Disaster Med Public Health Preparedness. 2017;11:15-20).

Intraerythrocytic iridovirus in central bearded dragons (Pogona vitticeps).

Three adult central bearded dragons (Pogona vitticeps) originating from a commercial breeding facility presented with clinical signs, including anorexia, dehydration, white multifocal lesions on the dorsal aspect of the tongue, blepharospasm, and weight loss. In 1 of 3 lizards, a marked regenerative anemia was noted, and all 3 bearded dragons had erythrocytic intracytoplasmic inclusion bodies. Nine bearded dragons housed in contact also had identical, but fewer intraerythrocytic inclusions. Inclusion bodies examined by electron microscopy had particles consistent with iridoviruses. Attempts to culture the virus were unsuccessful; however, amplification and sequencing of regions of the viral DNA polymerase by polymerase chain reaction confirmed the presence of an iridovirus. One of the bearded dragons died, while the 2 others showing clinical signs were euthanized. The remaining 9 infected bearded dragons of the teaching colony were also euthanized. Postmortem examination revealed a moderate, multifocal, lymphoplasmacytic or mononuclear adenitis of the tongue in the 3 bearded dragons, and a lymphohistiocytic hepatitis with bacterial granulomas in 2 lizards.

A new Isospora species of passerines in the family Turdidae from Costa Rica.

Seven thrush species (Turdidae) from Costa Rica were examined for intestinal parasites; 21 of the 84 (25%) birds sampled were positive for a new species of Isospora. Oocysts of Isospora zorzali n. sp. have thin, smooth, double, and colorless walls; they measure 19.7 ± 1.5 µm × 18.6 ± 1.4 µm (16-24 µm × 15-21 µm), with an average length-width ratio of 1.1 µm. Sporocysts are ovoid, measure 8.5 ± 1.1 µm × 14.5 ± 1.7 µm (7-11 µm × 11-18 µm) with an average length-width ratio of 1.7 µm. A nipple-like stieda body continuous with the sporocyst wall is present, but no substieda body was observed. A sporocyst residuum consisting of large equal sized granules was observed either clumped together or diffusely. The sporocysts fill the entire oocysts with little to no open space observed. This is the first report of Isospora species from any of the sampled host species and also the first report from any species of thrush in Costa Rica.

Susceptibility of white-tailed deer (Odocoileus virginianus) to experimental infection with epizootic hemorrhagic disease virus serotype 7.

During the fall of 2006, in Israel, epizootic hemorrhagic disease virus (EHDV) serotype 7 caused an intense and widespread epizootic in domestic cattle that resulted in significant economic losses for the dairy industry. The susceptibility of potential North American vector and ruminant hosts to infection with EHDV-7 is not known but is essential to understanding the potential for establishment of this exotic orbivirus in North America if it were introduced. Our primary objective was to determine whether white-tailed deer (WTD; Odocoileus virginianus) are susceptible to infection with EHDV-7. Six, 8-mo-old WTD were experimentally infected with EHDV-7, and all became infected and exhibited varying degrees of clinical disease. Clinical signs, clinicopathologic abnormalities, and postmortem findings were consistent with previous reports of orbiviral hemorrhagic disease (HD) in this species. Four of six animals died or were euthanized because of the severity of disease, one on postinoculation day (PID) 5 and the remaining WTD on PID 7. All deer had detectable viremia on PID 3, which peaked on PID 5 or 6 and persisted for as long as PID 46 in one animal. Deer surviving the acute phase of the disease seroconverted by PID 10. Based on the 67% mortality rate we observed, this strain of EHDV-7 is virulent in WTD, reaffirming their role as a sentinel species for the detection of endemic and nonendemic EHDV. Further, the observed disease was indistinguishable from previous reports of disease caused by North American EHDV and bluetongue virus serotypes, highlighting the importance of serotype-specific diagnostics during suspected HD outbreaks.

New species of Isospora from the blue-crowned motmot (Momotus momota) from Costa Rica.

Two (33.3%) of 6 blue-crowned motmots (Momotus momota) sampled from Costa Rica were positive for a new species of Isospora. Oocysts of Isospora momotana n. sp. have a double, thick, yellow to orange, 2.3 microm +/- 0.5 microm (2-3 microm) wall, contain 1 to 3 globular polar granules (1-4 microm), are ovoid with heavy pitting on the outer surface, and measure 29.4 +/- 2.3 x 27.5 +/- 2.3 (25-33 x 23-31) with an average length:width ratio of 1.1 (1.0-1.35). Sporocysts are ovoid, contain a residuum composed of large, equal-sized granules, and measure 19.4 +/- 1.3 x 12.2 +/- 1.1 (16-22 x 10-14) with an average length:width ratio of 1.6 (1.2-1.91). A small rounded stieda body, continuous with the sporocyst wall, and a prominent triangular substieda body are present. A second Isospora species was observed in 1 bird, but because only a few oocysts were present, a full description is not provided. This is the first report of coccidia from a motmot (Momotidae) and only the third Ispospora species described from the Coraciiformes.