Recording and erasure of photorefractive holograms in undoped BTO crystal at moderate to high intensities of 639.7 nm laser under action of 532 nm laser pre-illumination.

We investigate recording and erasure of photorefractive holographic gratings in an undoped Bi12TiO20 crystal in a moderate to high intensity regime of the recording beams at 639.7 nm without and with the action of laser pre-illumination at 532 nm. The detected hologram without pre-illumination indicates the participation of two photorefractive electronic gratings in its recording process, and the diffracted signal by itself exhibits a fivefold enhancement when the total intensity increases from 38.4 to 214.5 mW/cm2. The dependence of the measured total diffraction efficiency on intensity was investigated and showed linear behavior. At least three gratings are present in the regime of pre-illumination and participate in the writing and erasure of holographic mechanisms. Two of them are electronic, and one is hole-based, with a phase difference of Δϕ between them. The theoretical approach used to analyze the total diffraction efficiency based upon the photorefractivity standard model, and considering the presence of the three gratings, showed good agreement with the holographic erasure experimental data and permitted us to compute Δϕ, which exhibited strong and unusual dependence on the total intensity.

Role of the Tet38 Efflux Pump in Staphylococcus aureus Internalization and Survival in Epithelial Cells.

We previously identified the protein Tet38 as a chromosomally encoded efflux pump of Staphylococcus aureus that confers resistance to tetracycline and certain unsaturated fatty acids. Tet38 also contributes to mouse skin colonization. In this study, we discovered a novel regulator of tet38, named tetracycline regulator 21 (TetR21), that bound specifically to the tet38 promoter and repressed pump expression. A ΔtetR21 mutant showed a 5-fold increase in tet38 transcripts and an 8-fold increase in resistance to tetracycline and fatty acids. The global regulator MgrA bound to the tetR21 promoter and indirectly repressed the expression of tet38. To further assess the full role of Tet38 in S. aureus adaptability, we tested its effect on host cell invasion using A549 (lung) and HMEC-1 (heart) cell lines. We used S. aureus RN6390, its Δtet38, ΔtetR21, and ΔmgrA mutants, and a Δtet38 ΔtetR21 double mutant. After 2 h of contact, the Δtet38 mutant was internalized in 6-fold-lower numbers than RN6390 in A549 and HMEC-1 cells, and the ΔtetR21 mutant was internalized in 2-fold-higher numbers than RN6390. A slight increase of 1.5-fold in internalization was found for the ΔmgrA mutant. The growth patterns of RN6390 and the ΔmgrA and ΔtetR21 mutants within A549 cells were similar, while no growth was observed for the Δtet38 mutant. These data indicate that the Tet38 efflux pump is regulated by TetR21 and contributes to the ability of S. aureus to internalize and replicate within epithelial cells.

Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis.

This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida®. The antifungal activity of linalool, a monoterpene unsaturated constituent of basil oil, was performed using the broth microdilution technique. Then, the minimum inhibitory concentration (MIC), the two subsequent stronger concentrations and the positive controls were subcultured on Sabouraud Dextrose Agar plates to determine the minimum fungicidal concentration (MFC). The experiments were performed in triplicate and nystatin was used as a positive control in all tests. Diagnoses of oral candidiasis were verified in eight patients (66.6%) and the most prevalent fungal species was Candida albicans (37.5%), followed by Candida krusei (25.0%); and Candida tropicalis (4.2%). The best antifungal activity of linalool was observed on Candida tropicalis (MIC = 500 mg/mL), followed by Candida albicans (MIC = 1.000 mg/mL), and Candida krusei (MIC = 2.000 mg/mL).Under the study conditions and based on the results obtained, it can be concluded that the Candida strains tested were susceptible to linalool.

Evidence for genetic heterogeneity between clinical subtypes of bipolar disorder.

We performed a genome-wide association study of 6447 bipolar disorder (BD) cases and 12 639 controls from the International Cohort Collection for Bipolar Disorder (ICCBD). Meta-analysis was performed with prior results from the Psychiatric Genomics Consortium Bipolar Disorder Working Group for a combined sample of 13 902 cases and 19 279 controls. We identified eight genome-wide significant, associated regions, including a novel associated region on chromosome 10 (rs10884920; P=3.28 × 10-8) that includes the brain-enriched cytoskeleton protein adducin 3 (ADD3), a non-coding RNA, and a neuropeptide-specific aminopeptidase P (XPNPEP1). Our large sample size allowed us to test the heritability and genetic correlation of BD subtypes and investigate their genetic overlap with schizophrenia and major depressive disorder. We found a significant difference in heritability of the two most common forms of BD (BD I SNP-h2=0.35; BD II SNP-h2=0.25; P=0.02). The genetic correlation between BD I and BD II was 0.78, whereas the genetic correlation was 0.97 when BD cohorts containing both types were compared. In addition, we demonstrated a significantly greater load of polygenic risk alleles for schizophrenia and BD in patients with BD I compared with patients with BD II, and a greater load of schizophrenia risk alleles in patients with the bipolar type of schizoaffective disorder compared with patients with either BD I or BD II. These results point to a partial difference in the genetic architecture of BD subtypes as currently defined.

Screening for PS1 mutations in a referral-based series of AD cases: 21 novel mutations.

BACKGROUND: Mutations in the presenilin-1 gene (PS1) account for a majority of patients with early-onset familial AD. However, the clinical indications and algorithms for genetic testing in dementia are still evolving. METHODS: The entire open reading frame of the PS1 gene was sequenced in a series of 414 consecutive patients referred for diagnostic testing, including 372 patients with AD and 42 asymptomatic persons with a strong family history of AD. RESULTS: Forty-eight independent patients screened had a PS1 mutation including 21 novel mutations. In addition, 3% of subjects (11/413) had a known polymorphism, the Glu318Gly substitution. The majority of the mutations were missense substitutions but there were three insertions and Delta exon 10 mutation. With six exceptions (codons 35, 178, 352, 354, 358, and 365) most of the mutations occurred at residues conserved in the homologous PS2 gene or in PS1 of other species. CONCLUSIONS: Eleven percent of a referral-based series of patients with AD can be explained by coding sequence mutations in the PS1 gene. The high frequency of PS1 mutations in this study indicates that screening for PS1 mutations in AD is likely to be successful, especially when directed at patients with a positive family history with onset before 60 years (90% of those with PS1 mutations were affected by age 60 years). This will also have significance for the secondary identification of at-risk relatives who might be candidates for future prophylactic therapies for AD.

Evidence for linkage disequilibrium between the alpha 7-nicotinic receptor gene (CHRNA7) locus and schizophrenia in Azorean families.

Recent studies have suggested that the alpha 7-nicotinic receptor gene (CHRNA7) may play a role in the pathogenesis of schizophrenia. The alpha 7-nicotinic receptor gene (CHRNA7) is involved in P50 auditory sensory gating deficits, and the genomic locus for this gene lies in the chromosome 15q13-14 regions. The human gene is partially duplicated (exons 5-10) with four novel upstream exons. The marker D15S1360 has been shown to be significantly linked with the phenotype of abnormal P50 suppression in schizophrenia families. The marker L76630 is 3 kb in the 3' direction from the last exon of the CHRNA7 gene and is located in the duplicated region. The function of the two L76630 copies is unknown. We genotyped three polymorphic markers D15S1360, D15S165, and L76630 that are localized in a genomic fragment containing the CHRNA7 in 31 Azorean schizophrenia families/trios (including 41 schizophrenia individuals and 97 unaffected families members). An overall analysis utilizing the family-based association test revealed significant linkage disequilibrium between L76630 and schizophrenia (P = 0.0004). Using the extended transmission disequilibrium test and limiting the analysis to one triad per family, transmission disequilibrium of D15S1360 was near significance (P = 0.078). The 15q13 region overlaps with the location of two well-known genomically imprinted disorders: Angelman syndrome and Prader-Willi syndrome. Therefore, we investigated maternal and paternal meioses. We found significant transmission disequilibrium for D15S1360 through paternal transmission (P = 0.0006) in our schizophrenia families. The L76630 marker showed a significant disequilibrium in maternal transmissions (P = 0.028). No parent-of-origin effect was found in D15S165. Overall, our results suggest that the CHRNA7 may play a role in schizophrenia in these families. A parent of origin effect may be present and requires further study.

Long repeat tracts at SCA8 in major psychosis.

Expansion at a recently identified unstable trinucleotide repeat on chromosome 13q21 has been reported as the molecular cause for spinocerebellar ataxia type 8 (SCA8). The trinucleotide repeat, which consists of a [CTA]n repeat and adjacent [CTG]n repeat, was reported to have a pathogenic range of 107-127 CTG repeats (or 110-130 combined CTA and CTG repeats) in a large ataxia kindred. This repeat region was also cloned by our group from a bipolar affective disorder (BPAD) patient, who has approximately 600 combined repeats, and large alleles (>100 repeats) were reported to be present in 0.7% of controls and 1.5% of major psychosis patients (n = 710 and n = 1,120, respectively). We have followed up these findings by screening three new samples of BPAD and schizophrenia (SCZ) patients and controls, including 272 individuals from 14 BPAD families from Sweden, 130 individuals from 32 SCZ and BPAD families/trios from the Azores Islands, and 206 SCZ individuals from the United Kingdom and Ireland, and 219 matched controls. We found large repeat alleles above the SCA8 pathogenic range in individuals from 3 of 32 Azorean pedigrees and in 1 of 206 SCZ individuals from the United Kingdom, and repeat alleles within the SCA8 pathogenic range in 1 of 14 Swedish families. Although the rarity of major psychosis patients carrying the SCA8 expansion mutation would require a much larger sample size to reach statistical significance, these results support the previously reported observation of increased occurrence of large repeats at SCA8 in major psychosis. Am. J. Med. Genet. (Neuropsychiatr. Genet.) 96:873-876, 2000.

Umbilical vs peripheral vein catheterization for parenteral nutrition in sick premature neonates.

The efficacy and safety of using umbilical venous catheters vs. peripheral venous catheters for the delivery of parenteral nutrition was studied in 129 critically ill premature infants who were treated in a neonatal intensive care unit for the first 3 weeks of life. Infants who received parenteral nutrition by umbilical venous catheter had greater parenteral caloric intake, lower physiologic weight loss and greater weight gain during the study as compared to infants who received parenteral nutrition by peripheral vein. While the overall incidence of sepsis was comparable in both groups (19% vs 19.7%), benign and transient episodes of hyperglycemia were seen more commonly in infants receiving parenteral nutrition by umbilical catheters. None of the hyperglycemic infants, however, required insulin therapy. The incidence of other metabolic complication was comparable in both groups. At follow up, no evidence of portal hypertension was detected in any of the infants up to 66 months of age treated with umbilical venous catheters. We conclude that the use of umbilical venous catheter allows for a comparably safe and a more appropriate parenteral nutrition support than peripheral catheters in critically ill premature neonates.

[Susceptibility of Rhodnius neglectus, Rhodnius robustus and Triatoma infestans (Hemiptera, Reduviidae, Triatominae) to infection by two strains of Trypanosoma cruzi (Kinetoplastidae, Trypanosomatidae) using artificial xenodiagnosis]. / Susceptibilidade de Rhodnius neglectus, Rhodnius robustus et Triatoma infestans (Hemiptera, Reduviidae, Triatominae) à infecçao por duas cepas de Trypanosoma cruzi (Kinetoplastidae, Trypanosomatidae) utilizando xenodiagnóstico artificial.

The susceptibility of 3rd instar nymph of Triatominae Rhodnius neglectus, R. robustus and Triatoma infestans to Trypanosoma cruzi Y and AMJM strains was verified using artificial xenodiagnosis. After the accomplishment of the xenodiagnosis, the faeces of the Triatominae were analyzed on two-day intervals from day 5 until day 31 post infection, using the abdominal compression technique. The results showed differences in the susceptibility of the Triatominae for the two strains studied, and the optimal period reading differed from day 11 to day 19 for the Y strain and from day 11 to day 15 for the AMJM strain. For the Y strain, all three Triatominae species showed good susceptibility, whereas in the AMJM strain, the highest susceptibility was observed with R. neglectus, followed by T. infestans and R. robustus.

Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis / Atividade antifúngica do linalol em casos de Candida spp. isolados de indivíduos com candidíase oral

This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida®. The antifungal activity of linalool, a monoterpene unsaturated constituent of basil oil, was performed using the broth microdilution technique. Then, the minimum inhibitory concentration (MIC), the two subsequent stronger concentrations and the positive controls were subcultured on Sabouraud Dextrose Agar plates to determine the minimum fungicidal concentration (MFC). The experiments were performed in triplicate and nystatin was used as a positive control in all tests. Diagnoses of oral candidiasis were verified in eight patients (66.6%) and the most prevalent fungal species was Candida albicans (37.5%), followed by Candida krusei (25.0%); and Candida tropicalis (4.2%). The best antifungal activity of linalool was observed on Candida tropicalis (MIC = 500 mg/mL), followed by Candida albicans (MIC = 1.000 mg/mL), and Candida krusei (MIC = 2.000 mg/mL).Under the study conditions and based on the results obtained, it can be concluded that the Candida strains tested were susceptible to linalool.(AU)

Este estudo analisou a atividade antifúngica do fitoconstituinte linalol em cepas de Candida ssp, in vitro, isolados de pacientes com o diagnóstico clínico de candidíase oral associado ao uso de prótese dentária. As amostras biológicas foram coletadas de 12 pacientes portadores de próteses totais ou próteses parciais removíveis e que apresentavam características de mucosa eritematosa difusa ou pontilhadas, indicando um diagnóstico clínico de candidíase. Para identificar colônias de fungos do gênero Candida, as amostras foram semeadas em CHROMagar Candida®. A atividade antifúngica do linalol, um componente insaturado monoterpene de óleo de manjericão, foi realizada através da técnica de microdiluição em caldo. Em seguida, a concentração inibitória mínima (MIC), as duas concentrações consecutivas mais fortes e os controles positivos foram subcultivados em placas de Agar Sabouraud Dextrose para determinar a concentração fungicida mínima (MFC). Os experimentos foram realizados em triplicata e a nistatina foi usada como controle positivo em todos os testes. O diagnóstico de candidíase oral foi comprovado em oito pacientes (66,6%) e as espécies de fungos mais prevalentes foram Candida albicans (37,5%), seguido por Candida krusei (25,0%); e Candida tropicalis (4,2%). A melhor atividade antifúngica do linalol foi observada em Candida tropicalis (MIC = 500 mg/ml), seguido por Candida albicans (CIM = 1,000 mg/mL), e Candida krusei (CIM = 2,000 mg/mL). Sob as condições do estudo e com base nos resultados obtidos, pode-se concluir que as estirpes de Candida testadas foram susceptíveis a linalol.(AU)

Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis / Atividade antifúngica do linalol em casos de Candida spp. isolados de indivíduos com candidíase oral

Abstract This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida®. The antifungal activity of linalool, a monoterpene unsaturated constituent of basil oil, was performed using the broth microdilution technique. Then, the minimum inhibitory concentration (MIC), the two subsequent stronger concentrations and the positive controls were subcultured on Sabouraud Dextrose Agar plates to determine the minimum fungicidal concentration (MFC). The experiments were performed in triplicate and nystatin was used as a positive control in all tests. Diagnoses of oral candidiasis were verified in eight patients (66.6%) and the most prevalent fungal species was Candida albicans (37.5%), followed by Candida krusei (25.0%); and Candida tropicalis (4.2%). The best antifungal activity of linalool was observed on Candida tropicalis (MIC = 500 mg/mL), followed by Candida albicans (MIC = 1.000 mg/mL), and Candida krusei (MIC = 2.000 mg/mL).Under the study conditions and based on the results obtained, it can be concluded that the Candida strains tested were susceptible to linalool.

Resumo Este estudo analisou a atividade antifúngica do fitoconstituinte linalol em cepas de Candida ssp, in vitro, isolados de pacientes com o diagnóstico clínico de candidíase oral associado ao uso de prótese dentária. As amostras biológicas foram coletadas de 12 pacientes portadores de próteses totais ou próteses parciais removíveis e que apresentavam características de mucosa eritematosa difusa ou pontilhadas, indicando um diagnóstico clínico de candidíase. Para identificar colônias de fungos do gênero Candida, as amostras foram semeadas em CHROMagar Candida®. A atividade antifúngica do linalol, um componente insaturado monoterpene de óleo de manjericão, foi realizada através da técnica de microdiluição em caldo. Em seguida, a concentração inibitória mínima (MIC), as duas concentrações consecutivas mais fortes e os controles positivos foram subcultivados em placas de Agar Sabouraud Dextrose para determinar a concentração fungicida mínima (MFC). Os experimentos foram realizados em triplicata e a nistatina foi usada como controle positivo em todos os testes. O diagnóstico de candidíase oral foi comprovado em oito pacientes (66,6%) e as espécies de fungos mais prevalentes foram Candida albicans (37,5%), seguido por Candida krusei (25,0%); e Candida tropicalis (4,2%). A melhor atividade antifúngica do linalol foi observada em Candida tropicalis (MIC = 500 mg/ml), seguido por Candida albicans (CIM = 1,000 mg/mL), e Candida krusei (CIM = 2,000 mg/mL). Sob as condições do estudo e com base nos resultados obtidos, pode-se concluir que as estirpes de Candida testadas foram susceptíveis a linalol.

Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis

Abstract This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida®. The antifungal activity of linalool, a monoterpene unsaturated constituent of basil oil, was performed using the broth microdilution technique. Then, the minimum inhibitory concentration (MIC), the two subsequent stronger concentrations and the positive controls were subcultured on Sabouraud Dextrose Agar plates to determine the minimum fungicidal concentration (MFC). The experiments were performed in triplicate and nystatin was used as a positive control in all tests. Diagnoses of oral candidiasis were verified in eight patients (66.6%) and the most prevalent fungal species was Candida albicans (37.5%), followed by Candida krusei (25.0%); and Candida tropicalis (4.2%). The best antifungal activity of linalool was observed on Candida tropicalis (MIC = 500 mg/mL), followed by Candida albicans (MIC = 1.000 mg/mL), and Candida krusei (MIC = 2.000 mg/mL).Under the study conditions and based on the results obtained, it can be concluded that the Candida strains tested were susceptible to linalool.

Resumo Este estudo analisou a atividade antifúngica do fitoconstituinte linalol em cepas de Candida ssp, in vitro, isolados de pacientes com o diagnóstico clínico de candidíase oral associado ao uso de prótese dentária. As amostras biológicas foram coletadas de 12 pacientes portadores de próteses totais ou próteses parciais removíveis e que apresentavam características de mucosa eritematosa difusa ou pontilhadas, indicando um diagnóstico clínico de candidíase. Para identificar colônias de fungos do gênero Candida, as amostras foram semeadas em CHROMagar Candida®. A atividade antifúngica do linalol, um componente insaturado monoterpene de óleo de manjericão, foi realizada através da técnica de microdiluição em caldo. Em seguida, a concentração inibitória mínima (MIC), as duas concentrações consecutivas mais fortes e os controles positivos foram subcultivados em placas de Agar Sabouraud Dextrose para determinar a concentração fungicida mínima (MFC). Os experimentos foram realizados em triplicata e a nistatina foi usada como controle positivo em todos os testes. O diagnóstico de candidíase oral foi comprovado em oito pacientes (66,6%) e as espécies de fungos mais prevalentes foram Candida albicans (37,5%), seguido por Candida krusei (25,0%); e Candida tropicalis (4,2%). A melhor atividade antifúngica do linalol foi observada em Candida tropicalis (MIC = 500 mg/ml), seguido por Candida albicans (CIM = 1,000 mg/mL), e Candida krusei (CIM = 2,000 mg/mL). Sob as condições do estudo e com base nos resultados obtidos, pode-se concluir que as estirpes de Candida testadas foram susceptíveis a linalol.

Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis

Abstract This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida®. The antifungal activity of linalool, a monoterpene unsaturated constituent of basil oil, was performed using the broth microdilution technique. Then, the minimum inhibitory concentration (MIC), the two subsequent stronger concentrations and the positive controls were subcultured on Sabouraud Dextrose Agar plates to determine the minimum fungicidal concentration (MFC). The experiments were performed in triplicate and nystatin was used as a positive control in all tests. Diagnoses of oral candidiasis were verified in eight patients (66.6%) and the most prevalent fungal species was Candida albicans (37.5%), followed by Candida krusei (25.0%); and Candida tropicalis (4.2%). The best antifungal activity of linalool was observed on Candida tropicalis (MIC = 500 mg/mL), followed by Candida albicans (MIC = 1.000 mg/mL), and Candida krusei (MIC = 2.000 mg/mL).Under the study conditions and based on the results obtained, it can be concluded that the Candida strains tested were susceptible to linalool.

Resumo Este estudo analisou a atividade antifúngica do fitoconstituinte linalol em cepas de Candida ssp, in vitro, isolados de pacientes com o diagnóstico clínico de candidíase oral associado ao uso de prótese dentária. As amostras biológicas foram coletadas de 12 pacientes portadores de próteses totais ou próteses parciais removíveis e que apresentavam características de mucosa eritematosa difusa ou pontilhadas, indicando um diagnóstico clínico de candidíase. Para identificar colônias de fungos do gênero Candida, as amostras foram semeadas em CHROMagar Candida®. A atividade antifúngica do linalol, um componente insaturado monoterpene de óleo de manjericão, foi realizada através da técnica de microdiluição em caldo. Em seguida, a concentração inibitória mínima (MIC), as duas concentrações consecutivas mais fortes e os controles positivos foram subcultivados em placas de Agar Sabouraud Dextrose para determinar a concentração fungicida mínima (MFC). Os experimentos foram realizados em triplicata e a nistatina foi usada como controle positivo em todos os testes. O diagnóstico de candidíase oral foi comprovado em oito pacientes (66,6%) e as espécies de fungos mais prevalentes foram Candida albicans (37,5%), seguido por Candida krusei (25,0%); e Candida tropicalis (4,2%). A melhor atividade antifúngica do linalol foi observada em Candida tropicalis (MIC = 500 mg/ml), seguido por Candida albicans (CIM = 1,000 mg/mL), e Candida krusei (CIM = 2,000 mg/mL). Sob as condições do estudo e com base nos resultados obtidos, pode-se concluir que as estirpes de Candida testadas foram susceptíveis a linalol.

Avaliação qualitativa do leite pasteurizado tipo A, B e C comercializado em Natal, RN / Qualitative evaluation of type A, B and C pasteurized milk sold in Natal state of Rio Grande do Norte, Brazil

As amostras analisadas eram de oito marcas comerciais diferentes de leite pasteurizado comer-cializado na Cidade de Natal, RN. Para cada marca foram coletadas amostras de três diferentes datas de processamento, totalizando vinte e quatro amostras, sendo uma marca do tipo A, três do tipo B e quatro do tipo C. Foram realizadas análises microbiológicas (bactérias aeróbias mesófilas, coliformes totais e coliformes termotolerantes) e físico-químicas, estas através do aparelho de análise ultrassônica de leite, Ekomilk Total®, sendo determinadas: gordura, extrato seco desengordurado, densidade, proteína, ponto crioscópico e lactose. As amostras do leite tipo A apresentaram resultados fora dos padrões nas análises microbiológicas e físico-químicas. O leite pasteurizado tipo B obteve duas marcas com contagens microbiológicas em desacordo com a legislação. O leite pasteurizado tipo C foi o que apresentou os melhores resultados nas análises, embora três marcas tenham apresentado valores de coliformes totais acima do determinado pela legislação.(AU)

Eight different commercial brands of milk sold in Natal, state of Rio Grande do Norte, Brazil, were sampled and evaluated. Each brand was sampled based on 3 different processing dates, and a total of 24 samples were collected for 1, 3, and 4 brands of type A, B, and C milk, respectively. Analyses were carried out for microbiological measures (total mesophilic aerobic bacteria, total coliforms and thermo-tolerant coliforms) and physicochemical (Ekomilk Total® ultrasonic milk testing device) determinations were made for fat, nonfat dry extract, density, protein, cryoscopy point, and lactose. The results showed that type A pasteurized milk was the worst in terms of microbiological and physical-chemical properties. Two brands of type B pasteurized milk had microbiological counts violating the law. The type C milk had the best results in this trial although 3 out of 4 brands presented total coliform values above the Brazilian legislation.(AU)

Produção e qualidade do leite de cabras de torneiros leiteiros / Milk production quality in dairy goats that participate in dairy tournaments in the state of Rio Grande do Norte, Brazil

O objetivo deste trabalho foi avaliar a produção diária, a qualidade microbiológica e físico-químicas do leite das cabras participantes de Torneios Leiteiros no Estado do Rio Grande do Norte, Brasil. Foram analisadas 111 amostras de leite de 106 cabras (39 Saanen, 6 Toggenburg e 61 mestiças) agrupadas em categorias pela ordem de parto (primíparas e pluríparas) e estágio de lactação [inicial (0 a 30 dias), pico (30 a 45 dias) e pós-pico (mais de 45 dias)], utilizando o delineamento inteiramente casualisado. As cabras pluríparas apresentaram maiores médias de produção no início (4,41 kg/dia) e no pico (5,42 kg/dia) de lactação. Entre raças não houve diferença para produção media (4,62 kg/dia), ponto de congelamento (-0,488° C), pH (6,64), densidade (1,030 g/L), gordura (3,60%), proteína (2,90%), lactose (4,89%), extrato seco desengordurado (8,41%) e extrato seco total (12,06%). As análises microbiológicas revelaram a presença de mesófilos e coliformes fecais, sem diferença entre as ordens de parto ou entre raças. Cabras pluríparas apresentaram maiores produções de leite, mas não diferiram das primíparas quanto à composição, características físicas e microbiota do leite. As amostras de leite dos três genótipos (Saanen, Toggenburg e mestiças) analisados mostraram características físico-químicas, microbiológicas e de produção semelhantes.(AU)

The objective of this study was to evaluate the average daily production, the physicochemical components and microbiologicalagents in milk of primiparous and multiparous dairy goats that participate in dairy tournaments in the state of Rio Grande do Norte, Brazil. Analyses were carried out on 111 samples of goat milk from 106 goats (39 Saanen, 6 Toggenburg and 61 crossbred) grouped into categories by number of parity (primiparous and multiparous) and stage of lactation [initial (0 to 30 days), peak (30 to 45 days) and decrease (over 45 days)]. The data were analyzed in a completely randomizeddesign. The multiparous goats had higher average production at the beginning (4.41 kg/day) and peak (5.42 kg/day) of lactation. Between breeds there was no difference for average production(4.62 kg/day), freezing point (-0.488° C), pH (6.64), density (1.030 g/L), fat (3.60%), protein (2.90%) lactose (4.89%), dry extract (8.41%) or total solids (12.06%). Mesophiles (log CFU/mL) and fecal coliforms (log MPN/mL) were found in milk from goats, but no difference was observed between the orders of parity or between breeds. Multiparous goats had higher milk production, but did not differ from the primiparous goats regarding the composition, physical characteristics and microbiology of milk. The milk of 3 breeds (Saanen, Toggenburg and crossbred) presented similar physico-chemical, microbiological and production characteristics.(AU)

Produção e qualidade do leite de cabras de torneiros leiteiros / Milk production quality in dairy goats that participate in dairy tournaments in the state of Rio Grande do Norte, Brazil

O objetivo deste trabalho foi avaliar a produção diária, a qualidade microbiológica e físico-químicas do leite das cabras participantes de Torneios Leiteiros no Estado do Rio Grande do Norte, Brasil. Foram analisadas 111 amostras de leite de 106 cabras (39 Saanen, 6 Toggenburg e 61 mestiças) agrupadas em categorias pela ordem de parto (primíparas e pluríparas) e estágio de lactação [inicial (0 a 30 dias), pico (30 a 45 dias) e pós-pico (mais de 45 dias)], utilizando o delineamento inteiramente casualisado. As cabras pluríparas apresentaram maiores médias de produção no início (4,41 kg/dia) e no pico (5,42 kg/dia) de lactação. Entre raças não houve diferença para produção media (4,62 kg/dia), ponto de congelamento (-0,488° C), pH (6,64), densidade (1,030 g/L), gordura (3,60%), proteína (2,90%), lactose (4,89%), extrato seco desengordurado (8,41%) e extrato seco total (12,06%). As análises microbiológicas revelaram a presença de mesófilos e coliformes fecais, sem diferença entre as ordens de parto ou entre raças. Cabras pluríparas apresentaram maiores produções de leite, mas não diferiram das primíparas quanto à composição, características físicas e microbiota do leite. As amostras de leite dos três genótipos (Saanen, Toggenburg e mestiças) analisados mostraram características físico-químicas, microbiológicas e de produção semelhantes.

The objective of this study was to evaluate the average daily production, the physicochemical components and microbiologicalagents in milk of primiparous and multiparous dairy goats that participate in dairy tournaments in the state of Rio Grande do Norte, Brazil. Analyses were carried out on 111 samples of goat milk from 106 goats (39 Saanen, 6 Toggenburg and 61 crossbred) grouped into categories by number of parity (primiparous and multiparous) and stage of lactation [initial (0 to 30 days), peak (30 to 45 days) and decrease (over 45 days)]. The data were analyzed in a completely randomizeddesign. The multiparous goats had higher average production at the beginning (4.41 kg/day) and peak (5.42 kg/day) of lactation. Between breeds there was no difference for average production(4.62 kg/day), freezing point (-0.488° C), pH (6.64), density (1.030 g/L), fat (3.60%), protein (2.90%) lactose (4.89%), dry extract (8.41%) or total solids (12.06%). Mesophiles (log CFU/mL) and fecal coliforms (log MPN/mL) were found in milk from goats, but no difference was observed between the orders of parity or between breeds. Multiparous goats had higher milk production, but did not differ from the primiparous goats regarding the composition, physical characteristics and microbiology of milk. The milk of 3 breeds (Saanen, Toggenburg and crossbred) presented similar physico-chemical, microbiological and production characteristics.

Avaliação qualitativa do leite pasteurizado tipo A, B e C comercializado em Natal, RN / Qualitative evaluation of type A, B and C pasteurized milk sold in Natal state of Rio Grande do Norte, Brazil

As amostras analisadas eram de oito marcas comerciais diferentes de leite pasteurizado comer-cializado na Cidade de Natal, RN. Para cada marca foram coletadas amostras de três diferentes datas de processamento, totalizando vinte e quatro amostras, sendo uma marca do tipo A, três do tipo B e quatro do tipo C. Foram realizadas análises microbiológicas (bactérias aeróbias mesófilas, coliformes totais e coliformes termotolerantes) e físico-químicas, estas através do aparelho de análise ultrassônica de leite, Ekomilk Total®, sendo determinadas: gordura, extrato seco desengordurado, densidade, proteína, ponto crioscópico e lactose. As amostras do leite tipo A apresentaram resultados fora dos padrões nas análises microbiológicas e físico-químicas. O leite pasteurizado tipo B obteve duas marcas com contagens microbiológicas em desacordo com a legislação. O leite pasteurizado tipo C foi o que apresentou os melhores resultados nas análises, embora três marcas tenham apresentado valores de coliformes totais acima do determinado pela legislação.

Eight different commercial brands of milk sold in Natal, state of Rio Grande do Norte, Brazil, were sampled and evaluated. Each brand was sampled based on 3 different processing dates, and a total of 24 samples were collected for 1, 3, and 4 brands of type A, B, and C milk, respectively. Analyses were carried out for microbiological measures (total mesophilic aerobic bacteria, total coliforms and thermo-tolerant coliforms) and physicochemical (Ekomilk Total® ultrasonic milk testing device) determinations were made for fat, nonfat dry extract, density, protein, cryoscopy point, and lactose. The results showed that type A pasteurized milk was the worst in terms of microbiological and physical-chemical properties. Two brands of type B pasteurized milk had microbiological counts violating the law. The type C milk had the best results in this trial although 3 out of 4 brands presented total coliform values above the Brazilian legislation.

Avaliação qualitativa do leite pasteurizado tipo A, B, e C comercializado em Natal, RN

Eight different commercial brands of milk sold in Natal, state of Rio Grande do Norte, Brazil, were sampled and evaluated. Each brand was sampled based on 3 different processing dates, and a total of 24 samples were collected for 1, 3, and 4 brands of type A, B, and C milk, respectively. Analyses were carried out for microbiological measures (total mesophilic aerobic bacteria, total coliforms and thermo-tolerant coliforms) and physicochemical (Ekomilk Total® ultrasonic milk testing device) determinations were made for fat, nonfat dry extract, density, protein, cryoscopy point, and lactose. The results showed that type A pasteurized milk was the worst in terms of microbiological and physical-chemical properties. Two brands of type B pasteurized milk had microbiological counts violating the law. The type C milk had the best results in this trial although 3 out of 4 brands presented total coliform values above the Brazilian legislation.

As amostras analisadas eram de oito marcas comerciais diferentes de leite pasteurizado comer-cializado na Cidade de Natal, RN. Para cada marca foram coletadas amostras de três diferentes datas de processamento, totalizando vinte e quatro amostras, sendo uma marca do tipo A, três do tipo B e quatro do tipo C. Foram realizadas análises microbiológicas (bactérias aeróbias mesófilas, coliformes totais e coliformes termotolerantes) e físico-químicas, estas através do aparelho de análise ultrassônica de leite, Ekomilk Total®, sendo determinadas: gordura, extrato seco desengordurado, densidade, proteína, ponto crioscópico e lactose. As amostras do leite tipo A apresentaram resultados fora dos padrões nas análises microbiológicas e físico-químicas. O leite pasteurizado tipo B obteve duas marcas com contagens microbiológicas em desacordo com a legislação. O leite pasteurizado tipo C foi o que apresentou os melhores resultados nas análises, embora três marcas tenham apresentado valores de coliformes totais acima do determinado pela legislação.

Produção e qualidade do leite de cabras de torneios leiteiros

The objective of this study was to evaluate the average daily production, the physicochemical components and microbiological agents in milk of primiparous and multiparous dairy goats that participate in dairy tournaments in the state of Rio Grande do Norte, Brazil. Analyses were carried out on 111 samples of goat milk from 106 goats (39 Saanen, 6 Toggenburg and 61 crossbred) grouped into categories by number of parity (primiparous and multiparous) and stage of lactation [initial (0 to 30 days), peak (30 to 45 days) and decrease (over 45 days)]. The data were analyzed in a completely randomized design. The multiparous goats had higher average production at the beginning (4.41 kg/day) and peak (5.42 kg/day) of lactation. Between breeds there was no difference for average production (4.62 kg/day), freezing point (-0.488º C), pH (6.64), density (1.030 g/L), fat (3.60%), protein (2.90%) lactose (4.89%), dry extract (8.41%) or total solids (12.06%). Mesophiles (log CFU/mL) and fecal coliforms (log MPN/mL) were found in milk from goats, but no difference was observed between the orders of parity or between breeds. Multiparous goats had higher milk production, but did not differ from the primiparous goats regarding the composition, physical characteristics and microbiology of milk. The milk of 3 breeds (Saanen, Toggenburg and crossbred) presented similar physico-chemical, microbiological and production characteristics.

O objetivo deste trabalho foi avaliar a produção diária, a qualidade microbiológica e físico-químicas do leite das cabras participantes de Torneios Leiteiros no Estado do Rio Grande do Norte, Brasil. Foram analisadas 111 amostras de leite de 106 cabras (39 Saanen, 6 Toggenburg e 61 mestiças) agrupadas em categorias pela ordem de parto (primíparas e pluríparas) e estágio de lactação [inicial (0 a 30 dias), pico (30 a 45 dias) e pós-pico (mais de 45 dias)], utilizando o delineamento inteiramente casualisado. As cabras pluríparas apresentaram maiores médias de produção no início (4,41 kg/dia) e no pico (5,42 kg/dia) de lactação. Entre raças não houve diferença para produção media (4,62 kg/dia), ponto de congelamento (-0,488º C), pH (6,64), densidade (1,030 g/L), gordura (3,60%), proteína (2,90%), lactose (4,89%), extrato seco desengordurado (8,41%) e extrato seco total (12,06%). As análises microbiológicas revelaram a presença de mesófilos e coliformes fecais, sem diferença entre as ordens de parto ou entre raças. Cabras pluríparas apresentaram maiores produções de leite, mas não diferiram das primíparas quanto à composição, características físicas e microbiota do leite. As amostras de leite dos três genótipos (Saanen, Toggenburg e mestiças) analisados mostraram características físico-químicas, microbiológicas e de produção semelhantes.

Support for involvement of neuregulin 1 in schizophrenia pathophysiology.

Schizophrenia is a common, multigenic psychiatric disorder. Linkage studies, including a recent meta-analysis of genome scans, have repeatedly implicated chromosome 8p12-p23.1 in schizophrenia susceptibility. More recently, significant association with a candidate gene on 8p12, neuregulin 1 (NRG1), has been reported in several European and Chinese samples. We investigated NRG1 for association in schizophrenia patients of Portuguese descent to determine whether this gene is a risk factor in this population. We tested NRG1 markers and haplotypes for association in 111 parent-proband trios, 321 unrelated cases, and 242 control individuals. Associations were found with a haplotype that overlaps the risk haplotype originally reported in the Icelandic population ("Hap(ICE)"), and two haplotypes located in the 3' end of NRG1 (all P<0.05). However, association was not detected with Hap(ICE) itself. Comparison of NRG1 transcript expression in peripheral leukocytes from schizophrenia patients and unaffected siblings identified 3.8-fold higher levels of the SMDF variant in patients (P=0.039). Significant positive correlations (P<0.001) were found between SMDF and HRG-beta 2 expression and between HRG-gamma and ndf43 expression, suggesting common transcriptional regulation of NRG1 variants. In summary, our results suggest that haplotypes across NRG1 and multiple NRG1 variants are involved in schizophrenia.