1.
Science
; 262(5137): 1268-71, 1993 Nov 19.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8235657
RESUMO
A mutant mouse strain has been generated in which a rearranged immunoglobulin heavy (H) chain variable (V) region gene is placed into the heavy chain locus in its natural position, replacing the JH elements. In homozygous mutant mice, essentially all B cells in the spleen express the transgenic VH region in their antibodies. The proper location of the transgene relative to the constant region genes allows it to participate in isotype switching and undergo somatic hypermutation. Immunoglobulin transgenic mice generated in this fashion by gene targeting should prove useful for the exploration of immunoregulatory mechanisms.
Assuntos
Genes de Imunoglobulinas , Switching de Imunoglobulina , Cadeias Pesadas de Imunoglobulinas/genética , Região Variável de Imunoglobulina/genética , Sequência de Aminoácidos , Animais , Linfócitos B/imunologia , Sequência de Bases , Células Cultivadas , Homozigoto , Camundongos , Camundongos Transgênicos , Dados de Sequência Molecular , Mutação
2.
Eur J Immunol
; 26(8): 1966-9, 1996 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8765046