What Do Physicians Think About the Use of Telemedicine to Recruit and Assess Participants in mHealth-Related Clinical Studies as a Consequence of the COVID-19 Pandemic?

Objectives: To evaluate physician's opinion and availability to participate in mHealth-related clinical studies with patient recruitment and assessment via telemedicine and to identify characteristics associated with the willingness to participate. Methods: Cross-sectional, observational study, based on an anonymous web survey conducted in May-Jun of 2020 to 237 physicians, from Portugal and Spain that collaborated with an asthma mHealth project (INSPIRERS). Results: Response rate was 51% (n = 120). Most (74%, n = 89) physicians were available to participate in such studies, but 62% anticipated lower recruiting capacity and 40% increased difficulty in obtaining quality data. Physicians aged ≤40 years, from secondary care (vs. general practitioners) and that used apps in personal life or clinical practice were more likely to be available. Conclusions: Three-quarters of the physicians were available to participate in mHealth-related clinical studies with patient recruitment and assessment through telemedicine. Age group, medical specialty, and app use were associated with the willingness to participate.

Determinants of the Use of Health and Fitness Mobile Apps by Patients With Asthma: Secondary Analysis of Observational Studies.

BACKGROUND: Health and fitness apps have potential benefits to improve self-management and disease control among patients with asthma. However, inconsistent use rates have been reported across studies, regions, and health systems. A better understanding of the characteristics of users and nonusers is critical to design solutions that are effectively integrated in patients' daily lives, and to ensure that these equitably reach out to different groups of patients, thus improving rather than entrenching health inequities. OBJECTIVE: This study aimed to evaluate the use of general health and fitness apps by patients with asthma and to identify determinants of usage. METHODS: A secondary analysis of the INSPIRERS observational studies was conducted using data from face-to-face visits. Patients with a diagnosis of asthma were included between November 2017 and August 2020. Individual-level data were collected, including age, gender, marital status, educational level, health status, presence of anxiety and depression, postcode, socioeconomic level, digital literacy, use of health services, and use of health and fitness apps. Multivariate logistic regression was used to model the probability of being a health and fitness app user. Statistical analysis was performed in R. RESULTS: A total of 526 patients attended a face-to-face visit in the 49 recruiting centers and 514 had complete data. Most participants were ≤40 years old (66.4%), had at least 10 years of education (57.4%), and were in the 3 higher quintiles of the socioeconomic deprivation index (70.1%). The majority reported an overall good health status (visual analogue scale [VAS] score>70 in 93.1%) and the prevalence of anxiety and depression was 34.3% and 11.9%, respectively. The proportion of participants who reported using health and fitness mobile apps was 41.1% (n=211). Multivariate models revealed that single individuals and those with more than 10 years of education are more likely to use health and fitness mobile apps (adjusted odds ratio [aOR] 2.22, 95%CI 1.05-4.75 and aOR 1.95, 95%CI 1.12-3.45, respectively). Higher digital literacy scores were also associated with higher odds of being a user of health and fitness apps, with participants in the second, third, and fourth quartiles reporting aORs of 6.74 (95%CI 2.90-17.40), 10.30 (95%CI 4.28-27.56), and 11.52 (95%CI 4.78-30.87), respectively. Participants with depression symptoms had lower odds of using health and fitness apps (aOR 0.32, 95%CI 0.12-0.83). CONCLUSIONS: A better understanding of the barriers and enhancers of app use among patients with lower education, lower digital literacy, or depressive symptoms is key to design tailored interventions to ensure a sustained and equitable use of these technologies. Future studies should also assess users' general health-seeking behavior and their interest and concerns specifically about digital tools. These factors may impact both initial engagement and sustained use.

Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence.

Citrus is an extremely important genus in terms of world fruit production. Despite its economic importance and the small genome sizes of its species (2n = 18, 1C = 430 ± 68 Mbp), entire genomic assemblies have only recently become available for some of its representatives. Together with the previous CMA/DAPI banding and fluorescence in situ hybridization (FISH) in the group, these data are important for understanding the complex relationships between its species and for assisting breeding programs. To anchor genomic data with the cytogenetic map of mandarin (Citrus reticulata), the parental species of several economically important hybrids such as sweet orange and clementine, 18 BAC (bacterial artificial chromosome) clones were used. Eleven clementine BACs were positioned by BAC-FISH, doubling the number of chromosome markers so far available for BAC-FISH in citrus. Additionally, six previously mapped BACs were end-sequenced, allowing, together with one BAC previously sequenced, their assignment to scaffolds and the subsequent integration of chromosomes and the genome assembly. This study therefore established correlations between mandarin scaffolds and chromosomes, allowing further structural genomic and comparative study with the sweet orange genome, as well as insights into the chromosomal evolution of the group.

Melanin: Quantification and protection against oxidative stress in chromoblastomycosis agents.

Chromoblastomycosis (CBM) is a chronic cutaneous and subcutaneous infection caused by melanized fungal species. We quantified the extractable melanin of 77 strains of CBM agents distributed within five genera. Moreover, resistance to oxidative stress was evaluated in strains exposed or not to the melanin inhibitor tricyclazole. The median percentage of melanin mass extracted from dry fungal mass varied from 0.69 (Rhinocladiella similis) to 3.81 (Phialophora americana). Inhibition of melanin synthesis decreased survival rates to hydrogen peroxide. Together, these data highlight the importance of melanin in CBM agents.

South Brazilian wines: culturable yeasts associated to bottled wines produced in Rio Grande do Sul and Santa Catarina.

A comprehensive understanding of the presence and role of yeasts in bottled wines helps to know and control the organoleptic quality of the final product. The South Region of Brazil is an important wine producer, and the state of "Rio Grande do Sul" (RS) accounts for 90% of Brazilian wines. The state of "Santa Catarina" (SC) started the production in 1975, and is currently the fifth Brazilian producer. As there is little information about yeasts present in Brazilian wines, our main objective was to assess the composition of culturable yeasts associated to bottled wines produced in RS and SC, South of Brazil. We sampled 20 RS and 29 SC bottled wines produced between 2003 and 2011, and we isolated culturable yeasts in non-selective agar plates. We identified all isolates by sequencing of the D1/D2 domain of LSU rDNA or ITS1-5.8 S-ITS2 region, and comparison with type strain sequences deposited in GenBank database. Six yeast species were shared in the final product in both regions. We obtained two spoilage yeast profiles: RS with Zygosaccharomyces bailii and Pichia membranifaciens (Dekkera bruxellensis was found only in specific table wines); and SC with Dekkera bruxellensis and Pichia manshurica. Knowledge concerning the different spoilage profiles is important for winemaking practices in both regions.

Antifungal susceptibilities and identification of species of the Sporothrix schenckii complex isolated in Brazil.

Sporotrichosis is a subacute or chronic mycosis caused worldwide by the dimorphic species complex, Sporothrix schenckii. We studied 85 isolates recovered in Brazil to verify their identification and evaluate their in vitro antifungal susceptibility patterns. Based on phenotypic tests (microscopic features, ability to grow at 30°C and 37°C, colony diameters, as well as assimilation of sucrose and raffinose) and molecular assays (amplification of a fragment of the calmodulin gene), the strains were identified as S. schenckii, S. brasiliensis and S. globosa, with a predominance of S. schenckii isolates. There was 37.7% disagreement between the phenotypic and genotypic identification methodologies. In general, terbinafine was the most active drug, followed by ketoconazole and itraconazole, and the less active fluconazole and voriconazole. Five isolates (one S. globosa and four S. schenckii) were found to be itraconazole-resistant strains but, in general, there were no differences in the in vitro antifungal susceptibility profiles among the Sporothrix species.

A case of relapsed chromoblastomycosis due to Fonsecaea monophora: antifungal susceptibility and phylogenetic analysis.

Chromoblastomycosis is a chronic cutaneous and subcutaneous mycosis. The management of this infection continues to be challenging because there is no consensus on the therapeutic regimen. We report here a case of a 69-year-old male patient with cauliflower-like lesions on his left leg and foot. He had already been treated with itraconazole at a dose of 200 mg/day for 5 months, with mycological cure for all the affected areas. However, the lesions relapsed at both sites, and treatment with itraconazole was resumed at the dose previously used. Initially, direct mycological examination, cultural, and microculture slide observation were performed. Afterward, sequencing of the ITS1-5.8S rDNA-ITS2 region of the fungal DNA and evaluation of its susceptibility to antifungal agents alone and in combination were performed. In direct mycological examination, the presence of sclerotic cells was verified, and the fungus was identified as Fonsecaea based on cultural and microscopic examinations. Identification as Fonsecaea monophora was confirmed after sequencing of the ITS region and phylogenetic analysis. The isolate was susceptible to itraconazole and terbinafine. The combinations of amphotericin B and terbinafine and terbinafine and voriconazole were synergistic. The use of drugs for which the causative agent is susceptible to singly or in combination may be an alternative for the treatment of mycosis. Furthermore, the identification of the agent by molecular techniques is important for epidemiological purposes. To the best of our knowledge, this is the first case of relapsed chromoblastomycosis caused by F. monophora in Brazil.

Chromosome homeologies and high variation in heterochromatin distribution between Citrus L. and Poncirus Raf. as evidenced by comparative cytogenetic mapping.

The genus Citrus is well-known for its economic importance and complex taxonomy. Only three to six Citrus taxa are considered true biological species, among them is Citrus medica, the citron. Previous studies based on chromomycin A3 (CMA)/4',6-diamidino-2-phenylindole staining showed that the citron has a homomorphic karyotype, consisting of four distinct chromosome types according to the CMA(+) heterochromatin distribution. Based on the pattern of CMA(+) bands and the hybridization of 25 bacterial artificial chromosomes (BACs) from a genomic library of Poncirus trifoliata, a closely related species to the genus Citrus, we were able to identify each chromosome pair and build a comparative cytogenetic map for C. medica. The data showed a high degree of sequence conservation between these genera, enabling heterologous hybridization of BACs, and the establishment of chromosomal homeologies. It was thus possible to visualize changes in the position of some BACs in relation to CMA(+) bands. Since no breakdown of synteny was observed between these species, expansions and contractions in repetitive DNA sequences seem to be the major driving force of chromosomal evolution since the separation of these two genera.

[Shift work: overall health state related to sleep in nursing workers]. / Trabalho em turnos: estado geral de saúde relacionado ao sono em trabalhadores de enfermagem.

The objective of this study was to identify the symptoms related to the overall health status associated with shift work in nursing and relate it to the quality of sleep. The study was performed at Hospital da Irmandade da Santa Casa in Poços de Caldas, Minas Gerais State. The participants were 136 nursing professionals, of an average age of 33.1 years, divided into the following categories: nurse (8.1%); nurse technician (80.9%); and nursing aides working the day and night shifts (11%). The health symptoms were identified based on the Overall Health Status Inventory, and quality of sleep was evaluated based on sleep logs. The Chi-Square test showed statistical significance (p=0.021) for the presence of flatulence and abdominal distension during the night shift. Multiple linear regression analysis revealed that subjects working the day shift who experienced symptoms of poor digestion (sometimes or always) and irritability (always) had a poorer quality of sleep.

Psychological stress factors and salivary cortisol in nursing students throughout their training.

OBJECTIVE: to analyze psychological stress factors and salivary cortisol concentration in nursing undergraduates throughout their training. METHOD: a cross-sectional, analytical, and comparative study carried out in an evening course using a sociodemographic questionnaire, an Instrument to Assess Stress in Nursing Students, and salivary cortisol analysis. The study included descriptive and comparative analyses and a multiple linear regression model. RESULTS: 187 participants answered the questionnaires, and 129 had their cortisol quantified. The domains Practical Activities Execution, Professional Communication, and Professional Training represented the stress factors with the highest mean values for 3rd, 4th, and 5th-year students compared to 1st and 2nd year. For the 5th year, it was the domains Professional Communication and Professional Training compared to the 3rd year and Environment compared to the 1st and 3rd year. A significant result was obtained between the times of cortisol collections for males (p < 0.0001), females (p < 0.0001), and for 1st (p = 0.0319) 2nd (p = 0.0245), and 5th (p < 0.0001) years. CONCLUSION: Students in years 3 through 5 had higher exposure to stressors, and there were adjustments in cortisol production rhythmicity for students in years 1, 2, and 5.

Novel DOTA-neurotensin analogues for 111In scintigraphy and 68Ga PET imaging of neurotensin receptor-positive tumors.

Overexpression of the high affinity neurotensin receptor 1 (NTSR1), demonstrated in several human cancers, has been proposed as a new marker for human ductal pancreatic carcinoma and as an independent factor for poor prognosis for ductal breast cancer, head and neck squamous cell carcinoma, and non-small cell lung cancer. The aim of the present study was to develop new DOTA-neurotensin analogues for positron emission tomography (PET) imaging with (68)Ga and for targeted radiotherapy with (90)Y or (177)Lu. We synthesized a DOTA-neurotensin analogue series. Two of these peptides bear two sequence modifications for metabolic stability: DOTA-NT-20.3 shares the same peptide sequence as the previously described DTPA-NT-20.3. In the sequence of DOTA-NT-20.4, the Arg(8)-Arg(9) bond was N-methylated instead of the Pro(7)-Arg(8) bond in DOTA-NT-20.3. An additional sequence modification was introduced in DOTA-LB119 to increase stability. A spacer was added between DOTA and the peptide sequence to increase affinity. Binding to HT29 cells, which express NTSR1, in vivo stability, and biodistribution of the various analogues were compared, and the best candidate was used to image tumors of various sizes with the microPET in mice. (111)In-DOTA-NT-20.3, in spite of a relatively high uptake in kidneys, showed specific tumor uptake and elevated tumor to other organ uptake ratios. High contrast images were obtained at early time points after injection that allowed tumor detection at a time interval postinjection appropriate for imaging with the short-lived radionuclide (68)Ga. (111)In-DOTA-NT-20.4 displayed inferior binding to HT29 cells and reduced tumor uptake. (111)In-DOTA-LB119 displayed at early time points a significantly lower renal uptake but also a lower tumor uptake than (111)In-DOTA-NT-20.3, although binding to HT29 cells was similar. (68)Ga-DOTA-NT-20.3 displayed higher tumor uptake than (68)Ga-DOTA-LB119 and allowed the detection of very small tumors by PET. In conclusion, DOTA-NT-20.3 is a promising candidate for (68)Ga-PET imaging of neurotensin receptor-positive tumors. DOTA-NT-20.3 may also be considered for therapy, as the yttrium-labeled peptide has higher affinity than that of the indium-labeled one. A prerequisite for therapeutic application of this neurotensin analogue would be to lower kidney uptake, for example, by infusion of basic amino acids, gelofusin, or albumin fragments, to prevent nephrotoxicity, as with radiolabeled somatostatin analogues.

Profiling Persistent Asthma Phenotypes in Adolescents: A Longitudinal Diagnostic Evaluation from the INSPIRERS Studies.

We aimed to identify persistent asthma phenotypes among adolescents and to evaluate longitudinally asthma-related outcomes across phenotypes. Adolescents (13-17 years) from the prospective, observational, and multicenter INSPIRERS studies, conducted in Portugal and Spain, were included (n = 162). Latent class analysis was applied to demographic, environmental, and clinical variables, collected at a baseline medical visit. Longitudinal differences in clinical variables were assessed at a 4-month follow-up telephone contact (n = 128). Three classes/phenotypes of persistent asthma were identified. Adolescents in class 1 (n = 87) were highly symptomatic at baseline and presented the highest number of unscheduled healthcare visits per month and exacerbations per month, both at baseline and follow-up. Class 2 (n = 32) was characterized by female predominance, more frequent obesity, and uncontrolled upper/lower airways symptoms at baseline. At follow-up, there was a significant increase in the proportion of controlled lower airway symptoms (p < 0.001). Class 3 (n = 43) included mostly males with controlled lower airways symptoms; at follow-up, while keeping symptom control, there was a significant increase in exacerbations/month (p = 0.015). We have identified distinct phenotypes of persistent asthma in adolescents with different patterns in longitudinal asthma-related outcomes, supporting the importance of profiling asthma phenotypes in predicting disease outcomes that might inform targeted interventions and reduce future risk.

Monitoring Adherence to Asthma Inhalers Using the InspirerMundi App: Analysis of Real-World, Medium-Term Feasibility Studies.

Background: Poor medication adherence is a major challenge in asthma and objective assessment of inhaler adherence is needed. InspirerMundi app aims to monitor inhaler adherence while turning it into a positive experience through gamification and social support. Objective: We assessed the medium-term feasibility of the InspirerMundi app to monitor inhaler adherence in real-world patients with persistent asthma (treated with daily inhaled medication). In addition, we attempted to identify the characteristics of the patients related to higher app use. Methods: Two real-world multicenter observational studies, with one initial face-to-face visit and a 4-month telephone interview, were conducted in 29 secondary care centers from Portugal. During an initial face-to-face visit, patients were invited to use the app daily to register their asthma medication intakes. A scheduled intake was considered taken when patients took a photo of the medication (inhaler, blister, or others) using the image-based medication detection tool. Medication adherence was calculated as the number of doses taken as a percentage of the number scheduled. Interacting with the app ≥30 days was used as the cut-off for higher app use. Results: A total of 114 patients {median 20 [percentile 25 to percentile 75 (P25-P75) 16-36] years, 62% adults} were invited, 107 (94%) installed the app and 83 (73%) completed the 4-month interview. Patients interacted with the app for a median of 18 [3-45] days, translated on a median use rate of 15 [3-38]%. Median inhaler adherence assessed through the app was 34 [4-73]% when considering all scheduled inhalations for the study period. Inhaler adherence assessed was not significantly correlated with self-reported estimates. Median adherence for oral and other medication was 41 [6-83]% and 43 [3-73]%, respectively. Patients with higher app use were slightly older (p = 0.012), more frequently taking medication for other health conditions (p = 0.040), and more frequently prescribed long-acting muscarinic antagonists (LAMA, p = 0.024). After 4 months, Control of Allergic Rhinitis and Asthma Test (CARAT) scores improved (p < 0.001), but no differences between patients interacting with the app for 30 days or less were seen. Conclusions: The InspirerMundi app was feasible to monitor inhaler adherence in patients with persistent asthma. The persistent use of this mHealth technology varies widely. A better understanding of characteristics related to higher app use is still needed before effectiveness studies are undertaken.

Feasibility and Acceptability of an Asthma App to Monitor Medication Adherence: Mixed Methods Study.

BACKGROUND: Poor medication adherence is a major challenge in asthma, and objective assessment of inhaler adherence is needed. The InspirerMundi app aims to monitor adherence while providing a positive experience through gamification and social support. OBJECTIVE: This study aimed to evaluate the feasibility and acceptability of the InspirerMundi app to monitor medication adherence in adolescents and adults with persistent asthma (treated with daily inhaled medication). METHODS: A 1-month mixed method multicenter observational study was conducted in 26 secondary care centers from Portugal and Spain. During an initial face-to-face visit, physicians reported patients' asthma therapeutic plan in a structured questionnaire. During the visits, patients were invited to use the app daily to register their asthma medication intakes. A scheduled intake was considered taken when patients registered the intake (inhaler, blister, or other drug formulation) by using the image-based medication detection tool. At 1 month, patients were interviewed by phone, and app satisfaction was assessed on a 1 (low) to 5 (high) scale. Patients were also asked to point out the most and least preferred app features and make suggestions for future app improvements. RESULTS: A total of 107 patients (median 27 [P25-P75 14-40] years) were invited, 92.5% (99/107) installed the app, and 73.8% (79/107) completed the 1-month interview. Patients interacted with the app a median of 9 (P25-P75 1-24) days. At least one medication was registered in the app by 78% (77/99) of patients. A total of 53% (52/99) of participants registered all prescribed inhalers, and 34% (34/99) registered the complete asthma therapeutic plan. Median medication adherence was 75% (P25-P75 25%-90%) for inhalers and 82% (P25-P75 50%-94%) for other drug formulations. Patients were globally satisfied with the app, with 75% (59/79) scoring ≥4,; adherence monitoring, symptom monitoring, and gamification features being the most highly scored components; and the medication detection tool among the lowest scored. A total of 53% (42/79) of the patients stated that the app had motivated them to improve adherence to inhaled medication and 77% (61/79) would recommend the app to other patients. Patient feedback was reflected in 4 major themes: medication-related features (67/79, 85%), gamification and social network (33/79, 42%), symptom monitoring and physician communication (21/79, 27%), and other aspects (16/79, 20%). CONCLUSIONS: The InspirerMundi app was feasible and acceptable to monitor medication adherence in patients with asthma. Based on patient feedback and to increase the registering of medications, the therapeutic plan registration and medication detection tool were redesigned. Our results highlight the importance of patient participation to produce a patient-centered and engaging mHealth asthma app.

Chemical monitoring of river water bodies in an EU outermost region: examples from the Azores archipelago (Portugal).

Chemical monitoring of water quality on a total of 16 rivers in the Azores archipelago (Portugal), since 2003, made it possible to identify the major pressures and spatial geochemical variations along main course of the rivers. River water pollution is to a large extent associated to point sources, namely domestic wastewater discharges, especially in urban areas, and diffuse sources, associated with pasture land, and explain the high values on BOD(5) and nutrients (P and N). Heavy metals and metalloids, as well as hydrocarbons and pesticides, are generally under the detection limits of the analytical methods. Generally, river water reflects pollution loads according to a simple model, derived from land use in the watershed: in the upper part conditions are pristine, in the intermediate portion of the basin pasture land dominates and near the coast urban discharges are increasingly important. Results stress the role that an approach based on the watershed scale, coupled with land use management measures, are crucial to water management procedures and a successful WFD implementation in small river basin districts like the Azores. The paper also shows the need for full compliance regarding EU directives on urban wastewater and nitrate pollution due to agriculture.

Stress factors of nursing students in their final year.

OBJECTIVE: To identify the stress factors of the university environment and the repercussions on the quality of sleep and life of students in their last year of undergraduate nursing. METHOD: A cross-sectional, comparative, quantitative study conducted with students in their last year of undergraduate nursing in a private institution in the south of Minas Gerais. The Instrument for the Assessment of Stress in Nursing Students (ASNS), the Pittsburgh Sleep Quality Index (PSQI), the WHOQOL-Bref quality of life questionnaire and the sociodemographic characterization questionnaire were applied for data collection; association, comparison and correlation coefficient tests and descriptive statistics were performed for data analysis. RESULTS: The sample consisted of 55 participants. There was a strong correlation between Time Management of stress with sleep and the physical, psychological and environmental domains of quality of life, in addition to the significant association for insomnia and some sociodemographic variables. CONCLUSION: Stress factors were associated with poor quality sleep and changes in quality of life. Time Management was the factor with the greatest potential for stress. The ninth semester had greater exposure to stressors, worse quality of sleep and life.

Profile of nursing students: quality of life, sleep and eating habits.

OBJECTIVE: to profile and analyze sleep quality, quality of life and eating habits of nursing students. METHODS: a cross-sectional, comparative and correlational study with the following variables: sociodemographic characteristics, Pittsburgh sleep quality index, quality of life and eating habits. One hundred ninety-five students participated in the study. RESULTS: participants were on average 24 years old. Results showed that females were prevalent (87.1%); 71.0% of the students had poor sleep quality; 98.9% used electronic devices before bedtime. Self-perception of quality of life was indifferent (38.3%), and self-perception of general health, 36.9% expressed satisfaction. Among the participants, 45.13% consumed one portion of fruit and 40.66% two to three portions of vegetables daily. CONCLUSION: the study allowed the profile of these students, sedentary, overweight and poor sleep quality young students who studied and worked.

In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation.

In vivo hematopoietic generation occurs in waves of primitive and definitive cell emergence. Differentiation cultures of pluripotent embryonic stem cells (ESCs) offer an accessible source of hematopoietic cells for blood-related research and therapeutic strategies. However, despite many approaches, it remains a goal to robustly generate hematopoietic progenitor and stem cells (HP/SCs) in vitro from ESCs. This is partly due to the inability to efficiently promote, enrich, and/or molecularly direct hematopoietic emergence. Here, we use Gata2Venus (G2V) and Ly6a(SCA1)GFP (LG) reporter ESCs, derived from well-characterized mouse models of HP/SC emergence, to show that during in vitro differentiation they report emergent waves of primitive hematopoietic progenitor cells (HPCs), definitive HPCs, and B-lymphoid cell potential. These results, facilitated by enrichment of single and double reporter cells with HPC properties, demonstrate that in vitro ESC differentiation approximates the waves of hematopoietic cell generation found in vivo, thus raising possibilities for enrichment of rare ESC-derived HP/SCs.

Procedimento operacional padrão para armazenamento de materiais estéreis em ambiente extra hospitalar / Standard operating procedure for storage of sterile materials in extra-hospital environment

Introdução: Procedimento Operacional Padrão é um recurso de gestão, baseado em evidências científicas que auxilia no raciocínio e tomada de decisões de profissionais da área da saúde. Objetivo: Elaborar um Procedimento Operacional Padrão para armazenamento de materiais cirúrgicos estéreis em ambiente extra hospitalar, guiado por evidências científicas. Material e Métodos: Estudo metodológico descritivo baseado em uma revisão integrativa da literatura, cuja busca foi realizada nas bases National Library of Medicine, Red de Revistas Científicas de América Latina y el Caribe: Redalyc, Portal Regional da Biblioteca Virtual em Saúde, em setembro de 2023, com recorte temporal entre 2018 a 2023, além de busca no site do Ministério da Saúde. Resultados: Incluiram-se oito artigos, embasados na Resolução da Diretoria Colegiada, nº 15, de 2012. Os estudos abordaram temas sobre processamento de produtos para a saúde em ambiente hospitalar e não hospitalar, etapas do processamento de produtos para a saúde, segurança do paciente e educação permanente em saúde. A partir das evidências científicas elaborou-se o Procedimento Operacional Padrão, bem como um fluxograma. Conclusão: Pautado em evidências científicas e normativas instrucionais brasileiras, o Procedimento Operacional Padrão pode contribuir para um processo de trabalho qualificado, maior segurança na realização do cuidado e aprimoramento gerencial de materiais estéreis em ambiente extra hospitalar

Introduction: Standard Operating Procedure is a management resource, based on scientific evidence that assists in reasoning and decision-making by healthcare professionals. Objective: Develop a Standard Operating Procedure for storing sterile surgical materials in an extra-hospital environment, guided by scientific evidence. Material and Methods: Descriptive methodological study based on an integrative literature review, whose search was carried out in the National Library of Medicine, Red de Revistas Científicas de América Latina y el Caribe: Redalyc, Regional Portal of the Virtual Health Library, in September 2023 with clipping period between 2018 and 2023, in addition to a search on the Ministry of Health website. Results: Eight articles were included, based on the Resolution of the Collegiate Board, nº 15, of 2012. The studies addressed topics on the processing of health products in an environment hospital and non-hospital, stages of processing health products, patient safety and continuing health education. Based on the scientific evidence, the Standard Operating Procedure was created, as well as a flowchart. Conclusion: Based on scientific evidence and Brazilian instructional regulations, the Standard Operating Procedure can contribute to a qualified work process, greater safety in providing care and management improvements of sterile materials in an extra-hospital environment

Introducción: El Procedimiento Operativo Estándar es un recurso de gestión, basado en evidencia científica, que ayuda al razonamiento y la toma de decisiones de los profesionales de la salud. Objetivo: Desarrollar un Procedimiento Operativo Estándar para el almacenamiento de material quirúrgico estéril en un ambiente extrahospitalario, guiado por evidencia científica. Material y Métodos: Estudio metodológico descriptivo basado en una revisión integradora de la literatura, cuya búsqueda se realizó en la Biblioteca Nacional de Medicina, Red de Revistas Científicas de América Latina y el Caribe: Redalyc, Portal Regional de la Biblioteca Virtual en Salud, en septiembre de 2023 con periodo de recorte entre 2018 y 2023, además de una búsqueda en el sitio web del Ministerio de Salud. Resultados: Se incluyeron ocho artículos, con base en la Resolución del Consejo Colegiado, nº 15, de 2012. Los estudios abordaron temas sobre el procesamiento de productos para la salud en un ambiente hospitalario y no hospitalario, etapas de procesamiento de productos para la salud, seguridad del paciente y educación sanitaria continua. Con base en la evidencia científica se creó el Procedimiento Operativo Estándar, así como un diagrama de flujo. Conclusión: Basado en evidencia científica y normas de instrucción brasileñas, el Procedimiento Operativo Estándar puede contribuir para un proceso de trabajo calificado, mayor seguridad en la prestación de atención y mejoras en la gestión de materiales estériles en un ambiente extrahospitalario

Avaliação da comunicação interpessoal de profissionais de saúde em uma instituição de longa permanência para idosos / Evaluation of interpersonal communication of healthcare professionals in a long-stay institution for the elderly

Introdução: Em instituições de longa permanência para idosos a comunicação é um elemento fundamental para o relacionamento interpessoal entre os profissionais e moradores, a fim de proporcionar adequada compreensão do planoterapêutico e melhor desenvolvimento das relações de confiança e satisfação. Objetivo: Avaliar a comunicação interpessoal de profissionais de saúde em instituição de longa permanência para idosos. Materiais e Métodos: Trata-sede uma pesquisa transversal, descritiva, analítica com abordagem quantitativa, realizada em uma entidade filantrópicasem fins lucrativos de assistência e moradia à pessoa idosa - Lar São Vicente de Paulo, em um município no interior doestado de São Paulo. A coleta de dados ocorreu entre junho e julho de 2023. Utilizaram-se dois questionários, sendo um instrumento para dados sociodemográficos e a Escala de Competência em Comunicação Interpessoal para avaliar a comunicação dos profissionais de saúde. Foram realizadas análises descritivas e inferenciais. Resultados: Participaram 23 profissionais de saúde, 95,7% do sexo feminino, com média de idade de 45,2 anos, 91,3%, com renda mensal menor que três salários-mínimos e o mesmo percentual de profissionais que não possui outro trabalho. A pontuação mediana do escore total da Escala de Competência em Comunicação Interpessoal foi 64,8 pontos, com consistência interna média (Alpha de Cronbach= 0,58). Conclusões: Houve boa habilidade de comunicação interpessoal entre os profissionais de saúde, com diagnóstico situacional da comunicação interpessoal, além de conhecimento e possibilidade de aprimoramento dos processos de comunicação da equipe multidisciplinar, para maior qualidade e segurança no cuidado à pessoa idosa

Introduction: In long-term care institutions for elderly, communication is a fundamental element for the interpersonal relationship between professionals and residents, in order to provide adequate understanding of the therapeutic plan and better development of relationships of trust and satisfaction. Objective: To evaluate the interpersonal communication of health professionals in a long-term care institution for elderly. Materials and Methods: This is a cross-sectional, descriptive, analytical research with a quantitative approach, carried out in a non-profit philanthropic entity providing assistance and housing to elderly people - Lar São Vicente de Paulo, in a municipality of the State of São Paulo. Data collection occurred between June and July 2023. Two questionnaires were used, one instrument for sociodemographic data and the Interpersonal Communication Competence Scale to evaluate the communication of health professionals. Descriptive and inferential analyzes were carried out. Results: 23 health professionals participated, 95.7% female, with an average age of 45.2 years, 91.3% with a monthly income of less than three minimum wages, and the same percentage of professionals who did not have another job. The median score of the Interpersonal Communication Competence Scale total score was 64.8 points, with average internal consistency (Cronbach's Alpha= 0.58). Conclusions: There was good interpersonal communication skills among health professionals, with situational diagnosis of interpersonal communication, in addition to knowledge and the possibility of improving the communication processes of the multidisciplinary team, for greater quality and safety in the care of elderly people

Introduction: In long-term care institutions for elderly, communication is a fundamental element for the interpersonal relationship between professionals and residents, in order to provide adequate understanding of the therapeutic plan and better development of relationships of trust and satisfaction. Objective: To evaluate the interpersonal communication of health professionals in a long-term care institution for elderly. Materials and Methods: This is a cross-sectional, descriptive, analytical research with a quantitative approach, carried out in a non-profit philanthropic entity providing assistance and housing to elderly people - Lar São Vicente de Paulo, in a municipality of the State of São Paulo. Data collection occurred between June and July 2023. Two questionnaires were used, one instrument for sociodemographic data and the Interpersonal Communication Competence Scale to evaluate the communication of health professionals. Descriptive and inferential analyzes were carried out. Results: 23 health professionals participated, 95.7% female, with an average age of 45.2 years, 91.3% with a monthly income of less than three minimum wages, and the same percentage of professionals who did not have another job. The median score of the Interpersonal Communication Competence Scale total score was 64.8 points, with average internal consistency (Cronbach's Alpha= 0.58). Conclusions: There was good interpersonal communication skills among health professionals, with situational diagnosis of interpersonal communication, in addition to knowledge and the possibility of improving the communication processes of the multidisciplinary team, for greater quality and safety in the care of elderly people en un municipio del Estado de São Paulo. La recolección de datos se realizó entre junio y julio de 2023. Se utilizaron dos cuestionarios, un instrumento para datos sociodemográficos y la Escala de Competencia en Comunicación Interpersonal para evaluar la comunicación de los profesionales de la salud. Se realizaron análisis descriptivos e inferenciales. Resultados: Participaron 23 profesionales de la salud, 95,7% del sexo femenino, con edad promedio de 45,2 años, 91,3% con ingresos mensuales inferiores a tres salarios mínimos, e igual porcentaje de profesionales que no tenían otro trabajo. La mediana de la puntuación total en la Escala de Competencia en Comunicación Interpersonal fue de 64,8 puntos, con consistencia interna media (Alfa de Cronbach= 0,58). Conclusiones: Hubo buenas habilidades de comunicación interpersonal entre los profesionales de la salud, con diagnóstico situacional de la comunicación interpersonal, además de conocimiento y posibilidad de mejorar los procesos de comunicación del equipo multidisciplinario, para mayor calidad y seguridad en la atención a las personas mayores