RESUMO
Three-dimensional (3D) reconstruction of living brain tissue down to an individual synapse level would create opportunities for decoding the dynamics and structure-function relationships of the brain's complex and dense information processing network; however, this has been hindered by insufficient 3D resolution, inadequate signal-to-noise ratio and prohibitive light burden in optical imaging, whereas electron microscopy is inherently static. Here we solved these challenges by developing an integrated optical/machine-learning technology, LIONESS (live information-optimized nanoscopy enabling saturated segmentation). This leverages optical modifications to stimulated emission depletion microscopy in comprehensively, extracellularly labeled tissue and previous information on sample structure via machine learning to simultaneously achieve isotropic super-resolution, high signal-to-noise ratio and compatibility with living tissue. This allows dense deep-learning-based instance segmentation and 3D reconstruction at a synapse level, incorporating molecular, activity and morphodynamic information. LIONESS opens up avenues for studying the dynamic functional (nano-)architecture of living brain tissue.
Assuntos
Encéfalo , Sinapses , Microscopia de Fluorescência/métodos , Processamento de Imagem Assistida por ComputadorRESUMO
Cerrado is the second largest biome in Brazil, and it is responsible for providing us several ecosystem services, including the functions of storing Carbon and biodiversity conservation. In this study, we developed a modeling approach to predict the Aboveground biomass (AGB) in Cerrado vegetation using Artificial Neural Networks (ANNs), vegetation indices retrieved from RapidEye satellite imagery, and field data acquired within the Federal District territory, Brazil. Correlation testing was performed to identify potential vegetation index candidates to be used as input in the AGB modeling. Several ANNs were trained to predict the AGB in the study area using vegetation indices and field data. The optimum ANN was selected according to criteria of mean error of the estimate, correlation coefficient, and graphical analysis. The best performing ANN showed a predictive power of 90% and RMSE less than 17%. The validation tests showed no significant difference between the observed and ANN-predicted values. We estimated an average AGB of 16.55± 8.6 Mg.ha-1 in shrublands in the study area. Our study results indicate that vegetation indices and ANNs combined could accurately estimate the AGB in the Cerrado vegetation in the study area, showing to be a promising methodological approach to be broadly applied throughout the Cerrado biome.
Assuntos
Biomassa , Redes Neurais de Computação , Tecnologia de Sensoriamento Remoto , Brasil , Ecossistema , Monitoramento Ambiental/métodosRESUMO
The Amazon has a range of species with high potential for sustainable timber harvesting, but for them to be utilized globally, the merchantable wood volume must be accurately quantified. However, since the 1950s, inadequate methods for estimating merchantable timber volumes have been employed in the Amazon, and Brazilian Government agencies still require some of them. The natural variability of the Amazon Forest provides an abundance of species of different sizes and shapes, conferring several peculiarities, which makes it necessary to use up-to-date and precise methods for timber quantification in Amazon Forest management. Given the employment of insufficient estimation methods for wood volume, this study scrutinizes the disparities between the actual harvested merchantable wood volume and the volume estimated by the forest inventory during the harvesting phase across five distinct public forest areas operating under sustainable forest management concessions. We used mixed-effect models to evaluate the relationships between inventory and harvested volume for genera and forest regions. We performed an equivalence test to assess the similarity between the volumes obtained during the pre-and post-harvest phases. We calculated root mean square error and percentage bias for merchantable volume as accuracy metrics. There was a strong tendency for the 100% forest inventory to overestimate merchantable wood volume, regardless of genus and managed area. There was a significant discrepancy between the volumes inventoried and harvested in different regions intended for sustainable forest management, in which only 22% of the groups evaluated were equivalent. The methods currently practiced by forest companies for determining pre-harvest merchantable volume are inaccurate enough to support sustainable forest management in the Amazon. They may even facilitate the region's illegal timber extraction and organized crime.
Assuntos
Árvores , Madeira , Agricultura Florestal/métodos , Brasil , Conservação dos Recursos Naturais/métodos , FlorestasRESUMO
Although deforestation rates in the Brazilian Amazon are well known, the extent of the area affected by forest degradation is a notable data gap, with implications for conservation biology, carbon cycle science, and international policy. We generated a long-term spatially quantified assessment of forest degradation for the entire Brazilian Amazon from 1992 to 2014. We measured and mapped the full range of activities that degrade forests and evaluated the relationship with deforestation. From 1992 to 2014, the total area of degraded forest was 337,427 square kilometers (km2), compared with 308,311 km2 that were deforested. Forest degradation is a separate and increasing form of forest disturbance, and the area affected is now greater than that due to deforestation.
Assuntos
Biodiversidade , Conservação dos Recursos Naturais , Florestas , BrasilRESUMO
BACKGROUND: Long-term studies of community and population dynamics indicate that abrupt disturbances often catalyse changes in vegetation and carbon stocks. These disturbances include the opening of clearings, rainfall seasonality, and drought, as well as fire and direct human disturbance. Such events may be super-imposed on longer-term trends in disturbance, such as those associated with climate change (heating, drying), as well as resources. Intact neotropical forests have recently experienced increased drought frequency and fire occurrence, on top of pervasive increases in atmospheric CO2 concentrations, but we lack long-term records of responses to such changes especially in the critical transitional areas at the interface of forest and savanna biomes. Here, we present results from 20 years monitoring a valley forest (moist tropical forest outlier) in central Brazil. The forest has experienced multiple drought events and includes plots which have and which have not experienced fire. We focus on how forest structure (stem density and aboveground biomass carbon) and dynamics (stem and biomass mortality and recruitment) have responded to these disturbance regimes. RESULTS: Overall, the biomass carbon stock increased due to the growth of the trees already present in the forest, without any increase in the overall number of tree stems. Over time, both recruitment and especially mortality of trees tended to increase, and periods of prolonged drought in particular resulted in increased mortality rates of larger trees. This increased mortality was in turn responsible for a decline in aboveground carbon toward the end of the monitoring period. CONCLUSION: Prolonged droughts influence the mortality of large trees, leading to a decline in aboveground carbon stocks. Here, and in other neotropical forests, recent droughts are capable of shutting down and reversing biomass carbon sinks. These new results add to evidence that anthropogenic climate changes are already adversely impacting tropical forests.
RESUMO
An amendment to this paper has been published and can be accessed via the original article.
RESUMO
This work aimed to analyze possible differences in growth patterns on Eucalyptus species and to identify the determinants climatic variables on the growth. We evaluated six Eucalyptus species (Eucalyptus camaldulensis, Eucalyptus grandis, Eucalyptus urophylla, Eucalyptus saligna, Corymbia citriodora and Eucalyptus globulus) and a Clone (GG100) implanted in an experimental arrangement of randomized blocks. We collected the collar diameter and height of all plants monthly in the course of a year, in addition to climate data (minimum temperature, maximum and rainfall). Sequentially, we obtained the correlations between the current monthly increments (collar diameter and height) and climatic variables (minimum temperature, maximum and rainfall). The Current Monthly Increment of the Collar diameter (CMI D) was not correlated to the climatic variables evaluated and the Current Monthly Increment of the Height (CMI H) was strongly correlated to the minimum temperature for the species E. camaldulensis, C. citriodora, E. saligna, E. urophylla, E. grandis and the GG100 Clone. The Rainfall showed positive correlations regarding the CMI H only for the Clone (GG100) and E. urophylla. Finally, the species E. camaldulensis, E. urophylla, E. grandis, E. saligna presented a mortality rate under 10% which is recommended according to the silvicultural criteria.
Objetivou neste trabalho analisar possíveis diferenças nos padrões de crescimento em espécies de eucalipto e identificar as variáveis climáticas determinantes no crescimento. Foram avaliadas seis espécies de eucalipto (Eucalyptus camaldulensis, Eucalyptus grandis, Eucalyptus urophylla, Eucalyptus saligna, Corymbia citriodora e Eucalyptus globulus) e um Clone (GG100) implantados em arranjo experimental de blocos casualizados. Foram coletados o diâmetro do colo e altura de todas as plantas mensalmente no decorrer de um ano, além dos dados climáticos (temperatura mínima, máxima e precipitação). Sequencialmente foram obtidas correlações entre os incrementos correntes mensais (diâmetro do colo e altura) e as variáveis climáticas (temperatura mínima, máxima e precipitações). O Incremento Corrente Mensal do Diâmetro do Colo não apresentou correlação com as variáveis climáticas avaliadas e o Incremento Corrente Mensal em Altura (ICM H) é fortemente correlacionado com a temperatura mínima para as espécies E. camaldulensis, C. citriodora, E. saligna, E. urophylla, E. grandis e o Clone GG100. A precipitação apresentou correlações positiva com o ICM H somente para o Clone (GG100) e para E. urophylla. Por fim, as espécies E. camaldulensis, E. urophylla, E. grandis, E. saligna apresentaram taxa de mortalidade inferior a 10%, valor este recomendável de acordo com critérios silviculturais.
Assuntos
Estações do Ano , Mudança Climática , Pradaria , Eucalyptus/crescimento & desenvolvimentoRESUMO
This objective of this study was to characterize the floristic, structural, and ecological groups and to estimate the arboreal volume and biomass of a cerradão site in Palmas, Tocantins, Brazil. A forest inventory was conducted on 10.15 ha of the study area. Plots of 400-m2 were used for systematic sampling. All standing trees (dead or alive) with a breast-height diameter (DHB) greater than 5 cm were identified and measured. Floristic diversity and horizontal structure were assessed using the Shannon and importance value indices, respectively. Forest vertical structure was classified into three stratata and the tree species were categorized into ecological groups. Ninety tree volumes were rigorously cubed and weighed. Fresh- and dry biomass were sampled and estimated. Mathematical models were applied and adjusted to estimate tree volume and biomass. It was observed that the species Myrcia splendens and Emmotum nitens and the families Fabaceae and Chrysobalanaceae were dominant in our study site. The pioneer (613 individuals ha-1) and climax (530 individuals ha-1) tree species group predominated. The floristic diversity index was estimated as 3.35 nats ind- 1. The vertical structure analysis indicated fewer individuals in the superior stratum (13%) compared to the medium (63%) and inferior (24%) stratum. The Schumacher and Hall model showed better results with regard to estimated forest production.Forest volume and biomass estimates were 126.71 m³ ha-1 and 61.67 Mg ha-1, respectively. The studiedcerradão area had high floristic diversity and climax species predominated. Since this cerradão is inclose proximity to the Amazon biome, its volume and biomass stocks were higher than those estimatedfor other cerradão and forest formations within the Cerrado biome.(AU)
O objetivo do estudo foi caracterizar a florística, a estrutura, o grupo ecológico e estimar os estoques de volume e biomassa arbórea em uma área de 10, 15 hectares de cerradão, localizado no município de Palmas, Estado do Tocantins, utilizando a amostragem sistemática com parcelas de 400 m². Foram amostradas e identificadas todas as árvores vivas e mortas em pé, com diâmetro a 1,3 metros do solo ? 5 cm. A diversidade florística foi avaliada pelo o índice Shannon e a estrutura horizontal pelo o Índice de Valor de Importância. Quanto a estrutura vertical, a floresta foi classificada em três estratos. Sequencialmente as espécies foram classificadas em grupos ecológicos. Foram cubadas e pesadas 90 árvores. Obtida a massa verde, foram retiradas amostras para a obtenção da biomassa seca. Predominam na área as espécies Myrcia splendens e Emmotum nitens e, as famílias Fabaceae e Chrysobalanaceae. O grupo das espécies pioneiras (613 indivíduos ha-1) e clímax (530 indivíduos ha-1) se destacam. A diversidade florística foi de 3,35 nats.ind-1. Quanto aos estratos verticais, a menor quantidade de indivíduos é encontrada no superior (13%), a maior no médio (63%) e no inferior 24%. Para estimar a produção o modelo de Schumacher e Hall se sobressaiu. A quantidade de volume e biomassa estocado foram: 126,71 m³ha-1 e 61,67 Mg.ha-1, respectivamente. A área de cerradão estudada apresentou alta diversidade florística, com predominância de espécies clímax. Em função da sua proximidade com obioma Amazônico, os estoques de volume e biomassa estimados foram superiores em comparação comoutros cerradões e outras formações florestais dentro do bioma Cerrado.(AU)
Assuntos
Biomassa , Árvores/classificação , EcossistemaRESUMO
Os objetivos deste estudo foram ajustar e selecionar funções de densidade probabilística que caracterizem a estrutura diamétrica desse fragmento e das espécies: Cedrela fissilis (Cedro), Luehea divaricata (Açoita Cavalo), Gochnatia polymorpha (Cambará), Sebastiania commersoniana (Branquilho) e Casearia sylvestris (Cafezeiro) de um fragmento de Floresta Ombrófila Mista com 15,24ha, localizado em Curitiba, Paraná. Para avaliar a distribuição diamétrica dessa floresta e das cinco espécies selecionadas, foram ajustadas sete funções probabilísticas para intervalos de classe de 5cm. Segundo os critérios de seleção adotados, o modelo que melhor representou a distribuição diamétrica para toda a floresta, o cedro, o cambará e o branquilho foi o de Weber; para o cafezeiro, a função Gamma (Adaptada) foi a mais eficiente e para o açoita cavalo nenhuma dessas funções foi aderente. O branquilho, o açoita cavalo, o cafezeiro e a floresta como um todo apresentaram distribuição em "J" invertido ou decrescente. Entretanto, o cedro e o cambará apresentaram distribuição tendendo à unimodalidade com forte assimetria à direita.
The objective of this study was to adjust and select probability density functions that characterize the diametric structure of this forest fragment and for the species: Cedrela fissilis, Luehea divaricata, Gochnatia polymorpha, Sebastiania commersoniana and Casearia sylvestris for a 15.24 hectare fragment of Mixed Ombrophylous Forest, located in Curitiba, State of Paraná. In order to evaluate the diametric distributions of this forest and the five selected species, seven probability functions were adjusted for 5cm class intervals. According to the criteria of selection adopted, the model that best represented the diametric distributions for the whole forest, Cedrela fissilis, Gochnatia polymorpha and Sebastiania commersoniana was the Weber model; for Casearia sylvestris, the Gamma function (adapted) was the most efficient, and for Luehea divaricata, none of these functions showed adherence. The Sebastiania commersoniana, Luehea divaricata, Casearia sylvestris and the forest as a whole showed inverted "J" or decreasing distribution, while, Cedrela fissilis and Gochnatia polymorpha showed an almost unimodal distribution with a strong asymmetry to the right.
RESUMO
The objective of this study was to adjust and select probability density functions that characterize the diametric structure of this forest fragment and for the species: Cedrela fissilis, Luehea divaricata, Gochnatia polymorpha, Sebastiania commersoniana and Casearia sylvestris for a 15.24 hectare fragment of Mixed Ombrophylous Forest, located in Curitiba, State of Paraná. In order to evaluate the diametric distributions of this forest and the five selected species, seven probability functions were adjusted for 5cm class intervals. According to the criteria of selection adopted, the model that best represented the diametric distributions for the whole forest, Cedrela fissilis, Gochnatia polymorpha and Sebastiania commersoniana was the Weber model; for Casearia sylvestris, the Gamma function (adapted) was the most efficient, and for Luehea divaricata, none of these functions showed adherence. The Sebastiania commersoniana, Luehea divaricata, Casearia sylvestris and the forest as a whole showed inverted "J" or decreasing distribution, while, Cedrela fissilis and Gochnatia polymorpha showed an almost unimodal distribution with a strong asymmetry to the right.
Os objetivos deste estudo foram ajustar e selecionar funções de densidade probabilística que caracterizem a estrutura diamétrica desse fragmento e das espécies: Cedrela fissilis (Cedro), Luehea divaricata (Açoita Cavalo), Gochnatia polymorpha (Cambará), Sebastiania commersoniana (Branquilho) e Casearia sylvestris (Cafezeiro) de um fragmento de Floresta Ombrófila Mista com 15,24ha, localizado em Curitiba, Paraná. Para avaliar a distribuição diamétrica dessa floresta e das cinco espécies selecionadas, foram ajustadas sete funções probabilísticas para intervalos de classe de 5cm. Segundo os critérios de seleção adotados, o modelo que melhor representou a distribuição diamétrica para toda a floresta, o cedro, o cambará e o branquilho foi o de Weber; para o cafezeiro, a função Gamma (Adaptada) foi a mais eficiente e para o açoita cavalo nenhuma dessas funções foi aderente. O branquilho, o açoita cavalo, o cafezeiro e a floresta como um todo apresentaram distribuição em "J" invertido ou decrescente. Entretanto, o cedro e o cambará apresentaram distribuição tendendo à unimodalidade com forte assimetria à direita.
RESUMO
The objective of this study was to adjust and select probability density functions that characterize the diametric structure of this forest fragment and for the species: Cedrela fissilis, Luehea divaricata, Gochnatia polymorpha, Sebastiania commersoniana and Casearia sylvestris for a 15.24 hectare fragment of Mixed Ombrophylous Forest, located in Curitiba, State of Paraná. In order to evaluate the diametric distributions of this forest and the five selected species, seven probability functions were adjusted for 5cm class intervals. According to the criteria of selection adopted, the model that best represented the diametric distributions for the whole forest, Cedrela fissilis, Gochnatia polymorpha and Sebastiania commersoniana was the Weber model; for Casearia sylvestris, the Gamma function (adapted) was the most efficient, and for Luehea divaricata, none of these functions showed adherence. The Sebastiania commersoniana, Luehea divaricata, Casearia sylvestris and the forest as a whole showed inverted "J" or decreasing distribution, while, Cedrela fissilis and Gochnatia polymorpha showed an almost unimodal distribution with a strong asymmetry to the right.
Os objetivos deste estudo foram ajustar e selecionar funções de densidade probabilística que caracterizem a estrutura diamétrica desse fragmento e das espécies: Cedrela fissilis (Cedro), Luehea divaricata (Açoita Cavalo), Gochnatia polymorpha (Cambará), Sebastiania commersoniana (Branquilho) e Casearia sylvestris (Cafezeiro) de um fragmento de Floresta Ombrófila Mista com 15,24ha, localizado em Curitiba, Paraná. Para avaliar a distribuição diamétrica dessa floresta e das cinco espécies selecionadas, foram ajustadas sete funções probabilísticas para intervalos de classe de 5cm. Segundo os critérios de seleção adotados, o modelo que melhor representou a distribuição diamétrica para toda a floresta, o cedro, o cambará e o branquilho foi o de Weber; para o cafezeiro, a função Gamma (Adaptada) foi a mais eficiente e para o açoita cavalo nenhuma dessas funções foi aderente. O branquilho, o açoita cavalo, o cafezeiro e a floresta como um todo apresentaram distribuição em "J" invertido ou decrescente. Entretanto, o cedro e o cambará apresentaram distribuição tendendo à unimodalidade com forte assimetria à direita.
RESUMO
This objective of this study was to characterize the floristic, structural, and ecological groups and to estimate the arboreal volume and biomass of a cerradão site in Palmas, Tocantins, Brazil. A forest inventory was conducted on 10.15 ha of the study area. Plots of 400-m2 were used for systematic sampling. All standing trees (dead or alive) with a breast-height diameter (DHB) greater than 5 cm were identified and measured. Floristic diversity and horizontal structure were assessed using the Shannon and importance value indices, respectively. Forest vertical structure was classified into three stratata and the tree species were categorized into ecological groups. Ninety tree volumes were rigorously cubed and weighed. Fresh- and dry biomass were sampled and estimated. Mathematical models were applied and adjusted to estimate tree volume and biomass. It was observed that the species Myrcia splendens and Emmotum nitens and the families Fabaceae and Chrysobalanaceae were dominant in our study site. The pioneer (613 individuals ha-1) and climax (530 individuals ha-1) tree species group predominated. The floristic diversity index was estimated as 3.35 nats ind- 1. The vertical structure analysis indicated fewer individuals in the superior stratum (13%) compared to the medium (63%) and inferior (24%) stratum. The Schumacher and Hall model showed better results with regard to estimated forest production.Forest volume and biomass estimates were 126.71 m³ ha-1 and 61.67 Mg ha-1, respectively. The studiedcerradão area had high floristic diversity and climax species predominated. Since this cerradão is inclose proximity to the Amazon biome, its volume and biomass stocks were higher than those estimatedfor other cerradão and forest formations within the Cerrado biome.
O objetivo do estudo foi caracterizar a florística, a estrutura, o grupo ecológico e estimar os estoques de volume e biomassa arbórea em uma área de 10, 15 hectares de cerradão, localizado no município de Palmas, Estado do Tocantins, utilizando a amostragem sistemática com parcelas de 400 m². Foram amostradas e identificadas todas as árvores vivas e mortas em pé, com diâmetro a 1,3 metros do solo ? 5 cm. A diversidade florística foi avaliada pelo o índice Shannon e a estrutura horizontal pelo o Índice de Valor de Importância. Quanto a estrutura vertical, a floresta foi classificada em três estratos. Sequencialmente as espécies foram classificadas em grupos ecológicos. Foram cubadas e pesadas 90 árvores. Obtida a massa verde, foram retiradas amostras para a obtenção da biomassa seca. Predominam na área as espécies Myrcia splendens e Emmotum nitens e, as famílias Fabaceae e Chrysobalanaceae. O grupo das espécies pioneiras (613 indivíduos ha-1) e clímax (530 indivíduos ha-1) se destacam. A diversidade florística foi de 3,35 nats.ind-1. Quanto aos estratos verticais, a menor quantidade de indivíduos é encontrada no superior (13%), a maior no médio (63%) e no inferior 24%. Para estimar a produção o modelo de Schumacher e Hall se sobressaiu. A quantidade de volume e biomassa estocado foram: 126,71 m³ha-1 e 61,67 Mg.ha-1, respectivamente. A área de cerradão estudada apresentou alta diversidade florística, com predominância de espécies clímax. Em função da sua proximidade com obioma Amazônico, os estoques de volume e biomassa estimados foram superiores em comparação comoutros cerradões e outras formações florestais dentro do bioma Cerrado.