Novel dialkoxytriazine-type glycosyl donors for cellulase-catalysed lactosylation.

Novel glycosidic compounds, 4,6-dialkoxy-1,3,5-triazin-2-yl ß-lactosides (DAT-ß-Lac), have been prepared directly in water from lactose. The reaction was carried out on a laboratory scale without protecting the hydroxy groups of lactose. The resulting triazine derivatives were found to be recognized by endo-ß1,4-glucanase III from Trichoderma reesei (EGIII). The EGIII-catalysed transglycosylation of 4,6-dimethoxy-1,3,5-triazine derivative (DMT-ß-Lac) with various glycosyl acceptors has successfully been demonstrated, affording the corresponding lactosylated products.