How do plants remember drought?

MAIN CONCLUSION: Plants develop both short-term and transgenerational memory of drought stress through epigenetic regulation of transcription for a better response to subsequent exposure. Recurrent spells of droughts are more common than a single drought, with intermittent moist recovery intervals. While the detrimental effects of the first drought on plant structure and physiology are unavoidable, if survived, plants can memorize the first drought to present a more robust response to the following droughts. This includes a partial stomatal opening in the watered recovery interval, higher levels of osmoprotectants and ABA, and attenuation of photosynthesis in the subsequent exposure. Short-term drought memory is regulated by ABA and other phytohormone signaling with transcriptional memory behavior in various genes. High levels of methylated histones are deposited at the drought-tolerance genes. During the recovery interval, the RNA polymerase is stalled to be activated by a pause-breaking factor in the subsequent drought. Drought leads to DNA demethylation near drought-response genes, with genetic control of the process. Progenies of the drought-exposed plants can better adapt to drought owing to the inheritance of particular methylation patterns. However, a prolonged watered recovery interval leads to loss of drought memory, mediated by certain demethylases and chromatin accessibility factors. Small RNAs act as critical regulators of drought memory by altering transcript levels of drought-responsive target genes. Further studies in the future will throw more light on the genetic control of drought memory and the interplay of genetic and epigenetic factors in its inheritance. Plants from extreme environments can give queues to understanding robust memory responses at the ecosystem level.

Effect of inulin on textural and sensory characteristics of sorghum based high fibre biscuits using response surface methodology.

Five blends of sorghum (Sorghum bicolor) flour (25.0-45.0 %) and whole wheat flour were used to make biscuits using inulin (5.0-10.0 %) and guar gum (1.0-2.0 %). An experimental investigation was carried out with the aim of evaluating the effect of fat replacer (inulin) and sorghum flour on the quality of high fibre and low calorie biscuits. For this purpose, the biscuit dough and the biscuit samples were analyzed. The analysis was based on hardness of the dough, hardness of the biscuit, fracturability and overall acceptability (OAA) scores. Results showed that increasing the amount of sorghum flour in biscuit increased the dough hardness and biscuit hardness, whereas, biscuit fracturability decreased and OAA scores increased up to the level at which sorghum flour ranged from 35.0 to 40.0 % after which it decreased. An increase in the amount of inulin was followed by an increase in biscuit hardness, while, fracturability and OAA scores decreased and there was little effect on the dough hardness. Optimum conditions generated from the analysis was 40.8 % sorghum flour, 6.5 % inulin and 1.0 % guar gum. The predicted response in terms of dough hardness, fracturability, biscuit hardness and OAA were 212.4 g, 36.4 mm, 4.8 kg and 7.06, respectively. The desirability of the optimum condition was 0.827.

Development and optimization of electrosprayed vitamin C - chitosan nanoparticle: A CCD-RSM approach and characterization of bioactive encapsulant.

Electrospraying for Vitamin C (VC) encapsulation in Chitosan (Cs) nanoparticles was investigated and particle size, zeta potential, loading capacity (LC%) and encapsulation efficiency (EE%) were examined. Cs concentration (1-2% w/v) and voltage (21-25 kV) were varied with VC (0.25-0.75 w/w Cs). Twenty experiments in a face-centered CCD-RSM design were evaluated. ANOVA suggested voltage and Cs concentration as significant factors for particle size and VC content affected zeta, LC and EE%. RSM proposed optimum processing parameter at 2% Cs, 0.746 VC: Cs mass ratio and 21 kV voltage with 251.1 ± 59.03 nm particle size, 36.6% LC and an EE of 85.42%. Encapsulated particles were subjected to release behaviour, antioxidant property and analyzed through FTIR, DSC and XRD. Encapsulated VC had better antibacterial properties than Cs nanoparticles, and comparable VC retention in apple juice showed its effectiveness. Overall, nanoencapsulation of VC using electrospraying was successfully developed to be used in numerous food processing applications.

Encapsulation of probiotics in freeze-dried calcium alginate and κ-carrageenan beads using definitive screening design: A comprehensive characterisation and in vitro digestion study.

This research aimed to evaluate the encapsulation of the probiotic strain, Streptococcus thermophilus, in hydrogels employing sodium alginate (SA) with κ-carrageenan (κC) in gelation baths with varying salt concentrations (CaCl2 and KCl) followed by freeze-drying. The experimentation was conducted at varying levels of κC (0-0.5 % w/v) and SA (2-4 %). Freeze-dried hydrogels were evaluated based on encapsulation efficiency and loss of viability and further characterised. The study could successfully establish an encapsulation efficiency of 87.814 % and a viability loss of 1.201 log CFU·g-1 for the optimised samples. The SEM micrographs of the optimised Ca-alginate/κC hydrogels exhibited a much denser network with fewer pores. The influence of SA/κC in the beads was confirmed by FTIR and DSC, where distinct peak shifts were observed, which indicated the presence of κC and SA polymers. The probiotic survival under simulated gastrointestinal tract (GIT) conditions, performed in accordance with the INFOGEST protocol, indicated that the optimised Ca-alginate/κC beads had a lower rate of release in the gastric phase and a much higher rate of survival and release in the intestinal phase than the control sample. The swelling behaviour of beads varied due to varying pH in both gastric and intestinal phases, and the κC in the optimised beads affected the swelling ratio significantly.

Prediction of viscoelastic properties of peanut-based 3D printable food ink.

Viscoelastic properties of 3D printable peanut-based food ink were investigated using frequency sweep and relaxation test. The incorporation of xanthan gum (XG) improved the shear thinning behavior (n value ranging from 0.139 to 0.261) and lowered the η*, G', and G'' values, thus making food ink 3D printable. The addition of XG also caused a downward shift in the relaxation curve. This study evaluates the possibility of an artificial neural network (ANN) approach as a substitute for the Maxwell three-element and Peleg model for predicting the viscoelastic behavior of food ink. The results revealed that all three models accurately predicted the decay forces. The inclusion of XG decreased the hardness and enhanced the cohesiveness, so enabling the 3D printing of food ink. The hardness was highly positively correlated with Maxwell model parameters Fe , F1 , F2 , F3, and Peleg constant k2 (0.57) and negatively correlated with k1 (-0.76).

Onion dehydration: a review.

Onion (Allium cepa), a very commonly used vegetable, ranks third in the world production of major vegetables. Apart from imparting a delicious taste and flavour due to its pungency in many culinary preparations, it serves several medicinal purposes also. Processing and preservation of onion by suitable means is a major thrust area since a long time. The various kinds of treatments followed for dehydration of onion such as convective air drying, solar drying, fluidized bed drying, vacuum microwave drying, infrared drying and osmotic drying are reviewed here. These techniques are mainly used for preservation and value addition of onion. Several researchers have tried for decades to model the drying kinetics and quality parameters, which are also compiled here briefly.

Isolation and characterization of cellulose nanocrystals from Cucumis sativus peels.

Cucumber (Cucumis sativus) peels waste being a potential cellulosic sources, were used for extracting cellulose nanocrystals (CNCs), and characterized in the present study. Firstly, the cucumber peels were purified chemically through acid, alkali, and bleaching treatments for cellulose isolation. Later obtained cellulose was acid (60 wt% H2SO4) hydrolyzed at 45 ℃ for 45 min to obtain CNC45 suspension, and again 10 min sonicated for CNC45-S10 suspension. The effect of sonication on the particle size of CNC45, and CNC45-S10 were investigated with Dynamic light Scattering and Atomic force microscopy. The microstructural changes, thermal, and crystalline properties of resulting fibers and CNC45 were analysed after each treatments through scanning electron microscopy, thermo-gravimetric analyser, and X-ray diffraction respectively. The acid-hydrolysed CNC45 from cucumber peels showed rod-like shape with high crystallinity (74.1 %), excellent thermal stability (>200 °C), and negative zeta potential values (<-30 mV), and CNC45 can be used as potential nanofillers.

Understanding desiccation tolerance using the resurrection plant Boea hygrometrica as a model system.

Vegetative tissues of Boea hygrometrica, a member of the Gesneriaceae family, can tolerate severe water loss to desiccated state and fully recover upon rehydration. Unlike many other so called "resurrection plants," the detached leaves of B. hygrometrica also possess the same level of capacity for desiccation tolerance (DT) as that of whole plant. B. hygrometrica is distributed widely from the tropics to northern temperate regions in East Asia and grows vigorously in areas around limestone rocks, where dehydration occurs frequently, rapidly, and profoundly. The properties of detached B. hygrometrica leaves and relative ease of culture have made it a useful system to study the adaptive mechanisms of DT. Extensive studies have been conducted to identify the physiological, cellular, and molecular mechanisms underlying DT in the last decade, including specific responses to water stress, such as cell wall folding and pigment-protein complex stabilizing in desiccated leaves. In this review, the insight into the structural, physiological, and biochemical, and molecular alterations that accompany the acquisition of DT in B. hygrometrica is described. Finally a future perspective is proposed, with an emphasis on the emerging regulatory roles of retroelements and histone modifications in the acquisition of DT, and the need of establishment of genome sequence database and high throughput techniques to identify novel regulators for fully understanding of the matrix of DT.