A selective inhibitor of arachidonate 5-lipoxygenase scavenging peroxide activator.

A novel compound termed YT-18 (2,3-dihydro-2,4,6,7-tetramethyl-2-[(4-phenyl-1-piperazinyl) methyl]-5-benzofuranamine) selectively inhibited 5-lipoxygenases of porcine leukocytes (IC50 value, 7.5 microM), human leukocytes (1.5 microM), and rat basophilic leukemia cells (14 microM), which are responsible for bioactive leukotriene synthesis. In contrast, the compound up to 1 mM had almost no effect on 12-lipoxygenases of leukocytes and platelets, 15-lipoxygenase, and cyclooxygenases-1 and -2. YT-18 also inhibited the leukotriene synthesis in intact rat basophilic leukemia cells. In the 5-lipoxygenase reaction, YT-18 caused a lag phase, thereby delaying the start of the reaction. The lag was abolished by the addition of 13-hydroperoxy-linoleic acid in a dose-dependent manner, and most (but not all) of the reduced 5-lipoxygenase activity was recovered.

[Healing in the intestinal anastomosis--comparison of the Albert-Lembert and Gambee methods].

An experimental comparative histomorphological study was made on intestinal healing processes following an Albert-Lembert suture with approximation of the serosal surface and Gambee's layer to layer anastomosis of a dog. There was no obvious complications such as postoperative hemorrhage, anastomotic stenosis or anastomotic leakage. Although both anastomoses resulted in a good healing process, Gambee's layer to layer suture, which caused the submucosal layers to face each other, showed better wound healing at the anastomosis in terms of layer to layer attachment. As a conclusion, Gambee's layer to layer anastomosis seemed to be a better anastomotic technique in terms of wound healing for the intestinal anastomosis.