Identification of a genomic clone to ACC oxidase from papaya (Carica papaya L.) and expression studies.

In this paper are presented structural analysis and expression studies of one genomic clone encoding a 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) from papaya. Using RT-PCR amplification of ACC oxidase cDNAs from ripe papaya, a product of 800 bp was obtained, which after sequence analysis was found to code for a protein highly homologous to ACC oxidase proteins. This PCR product was used as a probe for screening a genomic library, and two different groups of clones were obtained as indicated by restriction mapping. One clone (CPACCO-1) was selected for further study and fully sequenced. Comparison of this sequence with the PCR product and other cloned ACC oxidase genes revealed that CPACCO-1 encoded the transcript in four exons interrupted by three introns. Southern blot analysis showed one or two major bands hybridized to the PCR probe, suggesting that the ACC oxidase gene is present in one or two copies in the papaya genome. By northern blot analysis it was found that the ACC oxidase transcripts appear in the pulp earlier than in the peel, suggesting a developmental regulation. A wounding experiment revealed the highest expression of this gene by 2 h. Transcriptional regulation by ethylene could be due to the presence of a putative GCC box in the promoter region.

Identificación organizacional y proactividad personal en grupos de trabajo: un modelo de ecuaciones estructurales / Organizational identification and personal proactivity in work groups: a structural equations model

El presente estudio explora la relación entre la identificación con la organización, las conductas de ciudadanía y la iniciativa personal dentro de los grupos de trabajo. Incluyendo una muestra de 336 empleados se prueba el ajuste de diferentes modelos causales a los datos. Los análisis de ecuaciones estructurales muestran que la identificación con la organización pronostica los resultados relativos a las conductas de ciudadanía organizacional y la iniciativa personal de los empleados pero que esta relación está parcialmente mediada por la cohesión grupal

The present study explores the relationships between organizational identification and both citizenship behavior and personal initiative within work groups. A sample of 336 employees was used to test the adjustment of different causal models to the data. Structural equations analyses showed that organizational identification predicts outcomes regarding employees’ organizational citizenship behaviors and personal initiative, but this relation was partially mediated by group cohesion