RESUMO
Chronic nonbacterial osteomyelitis (CNO) is an autoinflammatory bone disease that primarily affects children and adolescents. CNO is associated with pain, bone swelling, deformity, and fractures. Its pathophysiology is characterized by increased inflammasome assembly and imbalanced expression of cytokines. Treatment is currently based on personal experience, case series and resulting expert recommendations. Randomized controlled trials (RCTs) have not been initiated because of the rarity of CNO, expired patent protection of some medications, and the absence of agreed outcome measures. An international group of fourteen CNO experts and two patient/parent representatives was assembled to generate consensus to inform and conduct future RCTs. The exercise delivered consensus inclusion and exclusion criteria, patent protected (excludes TNF inhibitors) treatments of immediate interest (biological DMARDs targeting IL-1 and IL-17), primary (improvement of pain; physician global assessment) and secondary endpoints (improved MRI; improved PedCNO score which includes physician and patient global scores) for future RCTs in CNO.
Assuntos
Antirreumáticos , Osteomielite , Criança , Adolescente , Humanos , Consenso , Citocinas , Antirreumáticos/uso terapêutico , Osteomielite/tratamento farmacológico , Dor/complicações , Dor/tratamento farmacológico , Doença CrônicaRESUMO
BACKGROUND: Primary immunodeficiency is a rare disease of humoral and cellular immune defense, which can lead to severe and recurrent infections of different organs. The diagnosis of this disease is often difficult, and its early identification is necessary for adequate treatment and control. OBJECTIVE: This study aimed to analyze ear, nose, and throat (ENT) infections in adults and children with a primary immunodeficiency. We attempted to characterize possible warning signs that should trigger an immunologic diagnostic workup. MATERIALS AND METHODS: The current study comprised a retrospective case series of patients with primary immunodeficiencies. The type of immunodeficiency and the number of ENT infections were recorded. RESULTS: A total of 85 Patients were included in the study. 56 patients (66%) had an acute exacerbation of chronic rhinosinusitis (nâ¯= 28), cervical lymphadenitis (nâ¯= 16), acute tonsillitis (nâ¯= 14), and acute otitis media (nâ¯= 6). Reporting detailed information about the frequencies and dates of infections was not possible, due to the retrospective nature of the analysis. CONCLUSION: The prevalence of ENT infections in patients with a primary immunodeficiency is increased compared to the normal population. For the ENT specialist, these findings underline the necessity of including primary immunodeficiency in the differential diagnosis and initiating targeted diagnostic methods where indicated. Interdisciplinary collaboration with rheumatologists and immunologists is highly recommended, particularly for pediatric patients.
Assuntos
Síndromes de Imunodeficiência , Otite Média , Abscesso Peritonsilar , Sinusite , Adulto , Criança , Humanos , Síndromes de Imunodeficiência/complicações , Síndromes de Imunodeficiência/diagnóstico , Otite Média/imunologia , Abscesso Peritonsilar/imunologia , Estudos Retrospectivos , Sinusite/imunologiaRESUMO
The pathophysiology of chronic nonbacterial osteomyelitis (CNO) remains incompletely understood. Increased NLRP3 inflammasome activation and IL-1ß release in monocytes from CNO patients was suggested to contribute to bone inflammation. Here, we dissect immune cell infiltrates and demonstrate the involvement of monocytes across disease stages. Differences in cell density and immune cell composition may help to discriminate between BOM and CNO. However, differences are subtle and infiltrates vary in CNO. In contrast to other cells involved, monocytes are a stable element during all stages of CNO, which makes them a promising candidate in the search for "drivers" of inflammation. Furthermore, we link increased expression of inflammasome components NLRP3 and ASC in monocytes with site-specific DNA hypomethylation around the corresponding genes NLRP3 and PYCARD. Our observations deliver further evidence for the involvement of pro-inflammatory monocytes in the pathophysiology of CNO. Cellular and molecular alterations may serve as disease biomarkers and/or therapeutic targets.
Assuntos
Osso e Ossos/imunologia , Proteínas Adaptadoras de Sinalização CARD/genética , Inflamassomos/genética , Interleucina-1beta/genética , Monócitos/imunologia , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Osteomielite/imunologia , Infecções Bacterianas/genética , Infecções Bacterianas/imunologia , Infecções Bacterianas/metabolismo , Infecções Bacterianas/patologia , Western Blotting , Osso e Ossos/metabolismo , Osso e Ossos/patologia , Proteínas Adaptadoras de Sinalização CARD/imunologia , Proteínas Adaptadoras de Sinalização CARD/metabolismo , Estudos de Casos e Controles , Doença Crônica , Metilação de DNA , Regulação da Expressão Gênica , Humanos , Inflamassomos/imunologia , Inflamassomos/metabolismo , Inflamação , Interleucina-1beta/imunologia , Interleucina-1beta/metabolismo , Receptores de Lipopolissacarídeos/metabolismo , Monócitos/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/imunologia , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Osteomielite/genética , Osteomielite/metabolismo , Osteomielite/patologia , Reação em Cadeia da Polimerase em Tempo RealRESUMO
Chronic recurrent multifocal osteomyelitis (CRMO) is characterized by reduced activation of protein kinases ERK1 and 2 in monocytes resulting in impaired IL-10 expression. IL10 and its homologs IL19 and IL20 are organized in the IL10 cluster on chromosome 1q32. IL-10 and IL-19 are immune-regulatory cytokines, while IL-20 acts in a pro-inflammatory manner. The NLRP3 inflammasome, a multi-protein complex forming in response to innate stimuli, mediates IL-1ß cleavage and release. Here, we investigated IL-10-related cytokine expression in CRMO monocytes, underlying molecular events, and effects on inflammatory responses. We observed reduced anti-inflammatory IL-10 and IL-19 expression, and enhanced IL-20 expression in CRMO monocytes. Reduced IL-10 and IL-19 expression was associated with impaired Sp-1 recruitment to regulatory regions, contributing to NLRP3 inflammasome activation, which may induce inflammatory bone-loss. Our findings underscore the importance of balanced receptor-, cell-, and tissue-specific cytokine expression for immune homeostasis, providing additional arguments for cytokine blocking strategies in CRMO.
Assuntos
Expressão Gênica , Interleucina-10/genética , Interleucina-1beta/genética , Interleucinas/genética , Monócitos/metabolismo , Proteínas de Transporte/metabolismo , Linhagem Celular Tumoral , Células Cultivadas , Criança , Metilação de DNA , Ensaio de Imunoadsorção Enzimática , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Citometria de Fluxo , Humanos , Inflamassomos/metabolismo , Interleucina-10/metabolismo , Interleucina-1beta/metabolismo , Interleucinas/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR , Osteomielite/genética , Osteomielite/metabolismo , Osteomielite/patologia , Regiões Promotoras Genéticas/genética , Interferência de RNA , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fator de Transcrição Sp1/genética , Fator de Transcrição Sp1/metabolismoRESUMO
The introduction of cytokine-targeted therapies has significantly improved the treatment options of rheumatic diseases; however, some patients are also refractory to these treatment measures. The B cells play a central role in the pathogenesis of many rheumatic diseases and B-cell targeted therapies are a promising option as second-line medication for treating patients with a refractory disease course. Randomized controlled trials analyzing the efficacy of B-cell directed therapies for childhood rheumatic diseases have not yet been performed. The use of the B-cell depleting antibody rituximab showed positive results in non-controlled case series of juvenile systemic lupus erythematosus (SLE) patients. Patients with a refractory disease course of oligoarticular or polyarticular juvenile idiopathic arthritis might also benefit from B-cell depletion using rituximab. The B cell-targeting therapies for the treatment of childhood rheumatic diseases should be initiated and closely supervised by a pediatric rheumatologist.
Assuntos
Anticorpos Monoclonais Murinos/uso terapêutico , Linfócitos B/efeitos dos fármacos , Linfócitos B/imunologia , Doenças Reumáticas/tratamento farmacológico , Doenças Reumáticas/imunologia , Adolescente , Criança , Humanos , Doenças Reumáticas/patologia , RituximabRESUMO
Chronic non-bacterial osteomyelitis (CNO) is an auto-inflammatory disorder that affects the skeletal system. Interleukin (IL-)10 is an immune-modulatory cytokine that controls inflammation, and limits inflammatory cytokine responses. Dysregulation of IL-10 expression has been shown to result in autoimmune and infectious diseases. We investigated IL-10 expression by monocytic cells from CNO patients and controls. In response to stimulation with LPS, IL-10 expression from CNO monocytes was reduced (p<0.001). This was independent of IL10 promoter polymorphisms. Thus, we investigated Sp1 recruitment to the IL10 promoter and saw markedly reduced binding in CNO monocytes. This was accompanied with reduced phosphorylation of histone H3 serine 10 (H3S10), an activating epigenetic mark. Impaired recruitment of Sp1 to the IL10 promoter, and reduced H3S10 phosphorylation, may be a reflection of deficient MAPK signaling in CNO monocytes in response to LPS stimulation. Thus, we have discovered a mechanism that may be central in the pathophysiology of CNO.
Assuntos
Interleucina-10/genética , Sistema de Sinalização das MAP Quinases/imunologia , Osteomielite/imunologia , Fator de Transcrição Sp1/metabolismo , Células Cultivadas , Doença Crônica , Citocinas/biossíntese , Citocinas/sangue , Citocinas/imunologia , Histonas/imunologia , Histonas/metabolismo , Humanos , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/metabolismo , Lipopolissacarídeos/imunologia , Lipopolissacarídeos/farmacologia , Osteomielite/genética , Osteomielite/microbiologia , Fosforilação , Polimorfismo Genético , Regiões Promotoras Genéticas , Fator de Transcrição Sp1/genéticaRESUMO
Hypophosphatasia (HPP) is a rare inborn error of bone metabolism caused by various defects in the gene coding for the tissue-nonspecific alkaline phosphatase (TNSAP). It results in a reduced activity of the TNSAP and elevated concentrations of its substrates, including inorganic pyrophosphate. Clinical features of HPP include defective bone mineralisation with bone deformities, fractures and chronic non-bacterial osteomyelitis. Renal damage due to calcification, craniosynostosis and dental abnormalities with premature loss of dentition are further complications. Until now, detailed descriptions of whole-body magnetic resonance imaging (WB-MRI) in HPP do not exist. Here, we analysed WB-MRIs of 4 children with the childhood form of HPP. Deformities and defects of the long bones could be seen. All patients showed radiological lesions in the metaphyses of the long bones predominantly in the lower extremities being consistent with hyperaemia and oedema. Differential diagnosis includes an inflammatory process being active in these locations.
Assuntos
Hipofosfatasia/diagnóstico , Imageamento por Ressonância Magnética/métodos , Imagem Corporal Total/métodos , Calcinose/diagnóstico , Calcinose/genética , Calcinose/patologia , Pré-Escolar , Feminino , Humanos , Hipofosfatasia/genética , Hipofosfatasia/patologia , Lactente , Masculino , Osteomielite/diagnóstico , Osteomielite/patologiaRESUMO
The composition of the peripheral blood lymphocyte compartment underlies developmental changes during ontogeny. Recently, several new B cell populations have been characterized which were suggested to develop in an age-dependent manner. However, age-dependent reference values for distinct B cell populations have rarely been reported. Therefore, we have characterized developmental changes in peripheral B cell populations from infancy to adulthood in order to define age-dependent reference values. Using a flow cytometric approach we analysed the frequencies as well as the absolute counts of naive, switched and non-switched memory B cells, CD27-negative memory B cells, transitional B cells as well as CD21(low) CD38(low) B cells from neonates up to the age of 50 years. Most of the B cell subsets showed age-dependent developmental changes: while the peripheral B cell pool during infancy is characterized predominantly by transitional and naive B cells, the fraction of switched and non-switched memory B cells increases gradually with age. CD21(low) CD38(low) B cells as well as plasmablasts do not exhibit developmental changes. In summary, we could demonstrate particular changes in the peripheral blood B cell compartment during ontogeny. This study provides reference values of different B cell subpopulations offering comparability for studies addressing disturbed peripheral B cell development in immunodeficiency, autoimmunity or B cell reconstitution following cell-depleting therapies.
Assuntos
Envelhecimento/imunologia , Subpopulações de Linfócitos B/citologia , Contagem de Linfócitos , ADP-Ribosil Ciclase 1/metabolismo , Adolescente , Adulto , Antígenos CD19/metabolismo , Subpopulações de Linfócitos B/imunologia , Subpopulações de Linfócitos B/metabolismo , Antígeno CD24/metabolismo , Criança , Pré-Escolar , Humanos , Imunoglobulina D/metabolismo , Imunofenotipagem , Lactente , Antígenos Comuns de Leucócito/metabolismo , Glicoproteínas de Membrana/metabolismo , Pessoa de Meia-Idade , Receptores de Complemento 3d/metabolismo , Valores de Referência , Membro 7 da Superfamília de Receptores de Fatores de Necrose Tumoral/metabolismo , Adulto JovemRESUMO
Tumor necrosis factor alpha (TNFalpha) has broad effects on the immune system including lymphoid organ development as well as growth, survival und function of immune cells. TNFalpha has two main functions: regulatory effects and proinflammatory activities. In several diseases such as juvenile and adult "rheumatoid" arthritis, psoriasis and chronic inflammatory bowel disease, the application of TNFalpha-blocking medications has been beneficial. However, induction of inflammation in several organs including the eye, CNS, skin and gastrointestinal tract has been reported. We report on an 11-year-old girl with juvenile idiopathic arthritis, who developed Crohn's disease (CD) while taking etanercept for her arthritis. Etanercept was discontinued and an antibody-based anti-TNF treatment using adalimumab was started, which induced remission of the gastrointestinal symptoms promptly. This case indicates that immunodysregulatory and even proinflammatory effects of etanercept are of relevance in the clinical practice. Furthermore, TNFalpha as a part of its function seems to downregulate mucosal inflammation in CD.
Assuntos
Artrite Juvenil/tratamento farmacológico , Doença de Crohn/induzido quimicamente , Imunoglobulina G/efeitos adversos , Imunossupressores/efeitos adversos , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Adalimumab , Anti-Inflamatórios/uso terapêutico , Anticorpos Monoclonais/uso terapêutico , Anticorpos Monoclonais Humanizados , Criança , Doença de Crohn/tratamento farmacológico , Doença de Crohn/fisiopatologia , Etanercepte , Feminino , Fármacos Gastrointestinais/uso terapêutico , Humanos , Doença Iatrogênica , Intestinos/efeitos dos fármacos , Intestinos/imunologia , Intestinos/patologia , Receptores do Fator de Necrose Tumoral , Sulfassalazina/uso terapêutico , Resultado do Tratamento , Fator de Necrose Tumoral alfa/metabolismoRESUMO
The objectives of this study are autoinflammatory syndromes which are usually characterized by repeated attacks of fever, especially in children. The presentation of these diseases, however, varies between entities and between patients of a particular syndrome. We report a 16-year-old female patient, who suffered from periodic erythema and myositis/fasciitis. She experienced at least nine attacks of dermatitis and myositis, while no fever episodes were noted over a 3-year period. A delay of puberty with amenorrhea and a short stature were also present. Laboratory investigations consistently showed markedly increased inflammatory parameters (especially a high serum amyloid A) and dysproteinemia. Because the patient's mother complained about chronic and periodic abdominal pain with also persistently elevated inflammatory parameters, the differential diagnosis included hereditary disorders resulting in chronic inflammation. The diagnosis of an inherited tumor necrosis factor receptor (TNFR) 1-associated periodic syndrome (TRAPS) was confirmed by genetic analyses. Long-term anti-inflammatory treatment with etanercept resulted in a significant clinical improvement and reduction of the inflammatory parameters ESR, CRP, interleukin-6, TNF-α, and soluble TNF-α receptor 1, but not of interleukin-12. Monitoring of the cytokine profile suggested partial effectiveness of etanercept in the treatment of TRAPS. Hereditary fever syndromes have to be considered in case of chronic unexplained inflammation even if fever is no presenting symptom.
Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Citocinas/sangue , Imunoglobulina G/uso terapêutico , Receptores do Fator de Necrose Tumoral/uso terapêutico , Dor Abdominal/diagnóstico , Dor Abdominal/tratamento farmacológico , Dor Abdominal/genética , Adolescente , Eritema/sangue , Eritema/tratamento farmacológico , Eritema/genética , Etanercepte , Fasciite/sangue , Fasciite/tratamento farmacológico , Fasciite/genética , Feminino , Febre/sangue , Febre/diagnóstico , Febre/genética , Doenças Hereditárias Autoinflamatórias/sangue , Doenças Hereditárias Autoinflamatórias/tratamento farmacológico , Doenças Hereditárias Autoinflamatórias/genética , Humanos , Mutação , Miosite/sangue , Miosite/tratamento farmacológico , Miosite/genética , Receptores Tipo I de Fatores de Necrose Tumoral/genética , Proteína Amiloide A Sérica/metabolismoRESUMO
Hypophosphatasia (HP) is an inborn error of bone metabolism transmitted predominantly as an autosomal-recessive trait. It is characterized by a reduced activity of the tissue-nonspecific isoenzyme of alkaline phosphatase (TNSAP) and elevated concentrations of its substrates, including pyrophosphates. Clinical symptoms include defective bone mineralisation with bone deformities, fractures and as recently defined chronic non-bacterial osteomyelitis. Renal damage due to calcification, craniosynostosis and dental abnormalities with premature loss of dentition are further symptoms, which have been described as characteristic in the ESPED inquiry of 2004. Knowledge about the mechanisms underlying cell activation leading to inflammation and tissue destruction is still limited in HP. Recent investigations have provided evidence that calcium pyrophosphate crystals are essentially involved in activating inflammatory signal transduction pathways via different receptors of the innate immune system. Laboratory assays, genetic counselling and testing, and radiologic imaging can confirm the diagnosis. Because symptoms are highly variable in their clinical expression, patients should be followed by a HP-experienced multidisciplinary team (paediatrician, radiologist, orthopedist, neurosurgeon, dentist). At the moment symptomatic support and treatment is most important because a causative therapy, e. g. enzyme replacement therapy, is not yet available.
Assuntos
Doenças do Desenvolvimento Ósseo/diagnóstico , Hipofosfatasia/diagnóstico , Fosfatase Alcalina/deficiência , Fosfatase Alcalina/genética , Doenças do Desenvolvimento Ósseo/genética , Doenças do Desenvolvimento Ósseo/terapia , Criança , Pré-Escolar , Aberrações Cromossômicas , Comportamento Cooperativo , Genes Recessivos/genética , Humanos , Hipofosfatasia/genética , Hipofosfatasia/terapia , Lactente , Comunicação Interdisciplinar , Isoenzimas/deficiência , Isoenzimas/genética , Equipe de Assistência ao Paciente , FenótipoRESUMO
OBJECTIVE: Lyme borreliosis is characterized by strong inflammatory reactions probably due to the presence of Borrelia burgdorferi in the joint. It has been suggested that Borrelia induces the immunological mechanisms that either can amplify the inflammatory response or can suppress it. To reveal the underlying mechanisms of chemoattraction and activation of responding leukocytes, we investigated the induction of chemokines in human synoviocytes exposed to two different B. burgdorferi sensu stricto isolates (strain Geho and B31). METHODS: Synoviocytes were exposed in vitro up to 5 days. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was used to assess the relative chemokine mRNA expression of RANTES/CCL5, SDF-1alpha/CXCL12 alpha, SDF-1beta/CXCL12 beta, MCP-1/CCL2, MCP-2/CCL8, IL-8/CXCL8 and MIP-1alpha/CCL3, and enzyme-linked immunosorbant assay (ELISA) was used to assess the protein expression of RANTES, SDF-1, MCP-1, and MIP-1alpha in the culture supernatant. RESULTS: MCP-1 gene expression was not changed by strain B31 but MCP-1 gene expression along with protein concentration was suppressed by strain Geho. Both strains induced RANTES mRNA and protein concentration. SDF-1 gene expression was suppressed, whereas protein concentrations were unchanged by both strains. IL-8 gene expression was unchanged by using strain Geho but significantly upregulated by strain B31. Both strains induced MCP-2 mRNA expression. MIP-1alpha mRNA expression was induced, but chemokine concentration was suppressed by both strains. CONCLUSION: This study suggests that the orchestra of chemokines plays an important role in the immunopathogenesis of early Lyme arthritis.
Assuntos
Borrelia burgdorferi/imunologia , Quimiocinas/biossíntese , Doença de Lyme/imunologia , Membrana Sinovial/metabolismo , Borrelia burgdorferi/patogenicidade , Células Cultivadas , Quimiocinas/genética , Técnicas de Cocultura , Meios de Cultivo Condicionados/química , Meios de Cultivo Condicionados/metabolismo , Ensaio de Imunoadsorção Enzimática , Humanos , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Membrana Sinovial/citologia , Membrana Sinovial/imunologiaAssuntos
Anticorpos Antinucleares/sangue , Artrite Juvenil/imunologia , Citrulina/química , Peptídeos Cíclicos/sangue , Vimentina/imunologia , Adolescente , Artrite Juvenil/sangue , Artrite Juvenil/diagnóstico , Biomarcadores/sangue , Criança , Humanos , Mutação , Peptídeos Cíclicos/genética , Vimentina/química , Vimentina/genéticaRESUMO
Juvenile Idiopathic Arthritis (JIA) is the most common cause of chronic arthritis in childhood and adolescents and encompasses a heterogeneous group of different diseases. Due to the promising results of B-cell depleting therapies in rheumatoid arthritis the role of B-cells in autoimmune diseases has to be discussed in a new context. Additionally, experiments in mouse models have shed new light on the antibody-independent role of B-cells in the development of autoimmune diseases. In this review we will discuss the importance of B-cells in the pathogenesis of JIA appraising the question for an immunological basis of B-cell targeted therapy in JIA.
Assuntos
Propionatos/uso terapêutico , Doenças Reumáticas/tratamento farmacológico , Administração Oral , Adulto , Artrite Reumatoide/tratamento farmacológico , Ensaios Clínicos como Assunto , Avaliação de Medicamentos , Tolerância a Medicamentos , Feminino , Seguimentos , Humanos , Testes de Função Renal , Testes de Função Hepática , Masculino , Éteres Metílicos/efeitos adversos , Éteres Metílicos/uso terapêutico , Naftalenos/efeitos adversos , Naftalenos/uso terapêutico , Neuralgia/tratamento farmacológico , Osteoartrite/tratamento farmacológico , Propionatos/efeitos adversos , Transaminases/sangueRESUMO
Juvenile idiopathic arthritis (JIA) is the most common cause of chronic arthritis in childhood and adolescents and encompasses a heterogeneous group of diseases. The role of B cells (BC) in autoimmune diseases has been put in a new perspective due to the promising results of BC depleting therapies in RA. Experiments in mouse models have shed new light on the Ab-independent role of BC in the pathogenesis of autoimmune diseases. We discuss whether BC play a role in the pathogenesis of JIA appraising the question for an immunological basis of BC directed therapy.
Assuntos
Artrite Juvenil/imunologia , Linfócitos B/imunologia , Adolescente , Animais , Criança , Humanos , CamundongosRESUMO
Hypophosphatasia (HP) is a rare inborn error of bone and mineral metabolism characterized by a defect in the tissue non-specific alkaline phosphatase (TNSALP) gene. Calcium pyrophosphate dihydrate (CPPD) crystals are known to accumulate as substrates of TNSALP in tissues and joints of patients with HP. In CPPD-induced arthritis these crystals are known to induce an inflammatory response. HP patients do suffer from pain in their lower extremities. However, it is not clear whether CPPD crystals contribute to these musculoskeletal complaints in HP. As long as there is no curative treatment of HP, symptomatic treatment in order to improve clinical features, especially with regard to pain and physical activity, is of major interest to the patients. Knowledge of the mechanisms underlying crystal-induced cell activation, however, is limited. Here we describe recent advances in elucidating the signal transduction pathways activated by CPPD crystals as endogenous "danger signals". Recent investigations provided evidence that Toll/interleukin-1 receptor (TIR) domain containing receptors including Toll-like receptors (TLRs) and interleukin-1 receptor (IL-1R), as well as the triggering receptor expressed on myeloid cells 1 (TREM-1) and the NACHT-leucin rich repeat and pyrin-domain-containing protein (NALP3) containing inflammasome are essentially involved in acute CPPD crystal-induced inflammation. These receptors are considered in part as components of the innate immune system. Further studies are needed to improve our understanding of the pathophysiological mechanisms leading to inflammation and tissue destruction associated with deposition of microcrystals. They might support the development of new therapeutic strategies for crystal-induced inflammation. Eventually, patients with HP might as well profit from such strategies addressing these metabolic disorders secondary to the gene defect.
Assuntos
Pirofosfato de Cálcio/imunologia , Hipofosfatasia/imunologia , Doenças Reumáticas/imunologia , Proteínas de Transporte/imunologia , Humanos , Hipofosfatasia/fisiopatologia , Imunidade Inata/imunologia , Inflamação/imunologia , Inflamação/fisiopatologia , Proteína 3 que Contém Domínio de Pirina da Família NLR , Receptores de Interleucina-1/imunologia , Doenças Reumáticas/fisiopatologia , Receptores Toll-Like/imunologiaRESUMO
BACKGROUND: Re-expression of the recombination-activating genes (RAG) in peripheral B cells may be relevant in the development of autoreactive antibodies in autoimmune diseases. The presence of antinuclear antibodies (ANA) as a hallmark of oligoarticular juvenile idiopathic arthritis (o-JIA, early-onset type) indicates a breakdown in immunological tolerance. AIM: To examine the expression of RAG genes in peripheral blood mature B lymphocytes in patients with o-JIA. METHODS: 777 memory B cells from peripheral blood, CD19+ CD27+ CD5+ or CD19+ CD27+ CD5-, isolated from three ANA+ children with o-JIA and three healthy age-matched children, were examined for the expression of RAG1 and RAG2 mRNA. mRNA transcripts of activation-induced cytidine deaminase and immunoglobulin G were searched to further determine their developmental stage. RESULTS: mRNA was present for any of the two RAG genes in the B cells of children with JIA and controls. However, the predominance of RAG1 or RAG2 was different. A significantly decreased frequency of RAG2-expressing memory B cells in both CD5+ and CD5- populations was noted in children with JIA (p<0.001), whereas the number of RAG1-expressing B cells was slightly increased. The coordinate expression of both the RAG genes was a rare event, similar in the CD5+ populations (1% in controls, 2% in children with JIA), but different among the CD5- compartments (5% v 0%; p<0.01). CONCLUSION: These results argue for a reduced coordinate RAG expression in the peripheral CD5- memory B cells of patients with o-JIA. Thus, it was hypothesised that impaired receptor revision contributes to autoimmune pathogenesis in JIA.
Assuntos
Artrite Juvenil/imunologia , Subpopulações de Linfócitos B/metabolismo , Proteínas de Ligação a DNA/biossíntese , Proteínas de Homeodomínio/biossíntese , Proteínas Nucleares/biossíntese , Artrite Juvenil/genética , Antígenos CD5/sangue , Células Cultivadas , Pré-Escolar , Citidina Desaminase/biossíntese , Citidina Desaminase/genética , Proteínas de Ligação a DNA/genética , Expressão Gênica , Genes RAG-1 , Proteínas de Homeodomínio/genética , Humanos , Imunoglobulina G/biossíntese , Imunoglobulina G/genética , Memória Imunológica , Imunofenotipagem , Proteínas Nucleares/genética , Reação em Cadeia da Polimerase/métodos , RNA Mensageiro/genética , Membro 7 da Superfamília de Receptores de Fatores de Necrose Tumoral/sangueRESUMO
BACKGROUND: The assembly of immunoglobulin genes during B cell development in the bone marrow is dependent on the expression of recombination activating genes (RAG) 1 and 2. Recently, RAG expression in peripheral blood IgD+ B cells outside the bone marrow has been demonstrated and is associated with the development of autoimmune diseases. OBJECTIVE: To investigate RAG expression in the CD5+ or CD5- IgD+ B cell compartment in childhood systemic lupus erythematosus (SLE). METHODS: Using a combination of flow cytometric cell sorting and reverse transcriptase polymerase chain reaction analysis of cDNA libraries generated from individual cells, the expression of RAG, VpreB, and CD154 mRNA by individual peripheral blood B cells of three paediatric SLE patients was examined in detail. RESULTS: While only one patient had a significantly increased frequency of RAG+ B cells in the CD5- B cell population, all patients showed higher frequencies of RAG+ B cells in the CD5+IgD+ B cell population. The frequency of RAG+ IgD+CD5+/- B cells was reduced during intravenous cyclophosphamide treatment. In healthy age matched children, RAG expressing IgD+ B cells were hardly detectable. Coexpression of RAG and VpreB or CD154 mRNA could only be found in SLE B cells. CONCLUSIONS: RAG expression in peripheral blood B cells of SLE patients is particularly increased in the IgD+CD5+ B cell population. CD5+ and CD5- B cells in SLE have the potential to undergo receptor revision leading to the generation of high affinity pathogenic autoantibodies.