Antimicrobial resistance profile of non-aureus Staphylococci isolates from buffalo, goat and sheep mastitis in the Northeast region of Brazil.

The study described in this Research Communication investigated the genotypic and phenotypic profiles of resistance to beta-lactams and other antimicrobials in non-aureus Staphylococci (NAS) isolated from buffalo, goat and sheep mastitis in the Northeast region of Brazil. A total of 190 isolates were analyzed and 42.3, 43.9 and 23.6% of them were positive for blaZ gene in buffalo, goat and sheep, respectively. Regarding the animal groups, in goats, amoxicillin was the antimicrobial with highest resistance index (72.7%), followed by penicillin G in buffaloes (51.9%) and ampicillin in sheep (43.1%). With regard to multiple antimicrobial resistance, 30.8% of NAS isolates from buffalo milk samples, 25.8% from goats and 25.0% from sheep presented multidrug-resistance. In the minimum inhibitory concentration (MIC) technique, amoxicillin MIC50 and MIC90 were 64 and 128 µg/ml, respectively, among isolates of the three animal species. In conclusion, high rates of resistance to beta-lactams are presented among NAS isolated from mastitis cases in buffaloes, goats and sheep in Northeast region of Brazil. These results provide an alert to animal and human health researchers, suggesting that the frequency of NAS needs to be reduced because they carry resistance genes which might increase the existing levels of antimicrobial resistance.

Catarrhal mastitis by Staphylococcus simulans in a nulliparous goat / Mastite catarral causada por Staphylococcus simulans em uma cabra nulipara

The present paper is a case report of a one-year old nulliparous Alpine Goat belonging to a dairy goat farm in semi-arid region of Brazil. Both glands were naturally infected by α-hemolytic Staphylococcus simulans and evolved similar clinical signs. The mammary glands presented an acute catarrhal mastitis with systemic clinical signs that responded positively to treatment with gentamicin associated with amoxicillin. The present report suggests the importance of the pathogenic potential of non-aureus Staphylococci strains (NAS) as a cause of clinical mastitis also in nulliparous animals. The isolate showed resistance to tetracycline and contained staphylococcal toxin production genes (sec, sec and TSST-1). Moreover, it has been reported that Staphylococcus simulans is an emerging pathogen in humans causing cutaneous and osteoarticular infections, mainly in those in close contact with farm animals. To the best of our knowledge, this is the first report of a clinical mastitis in a nulliparous goat caused by Staphylococcus simulans(AU)

O presente trabalho é o relato do caso de uma cabra nulípara da raça Parda Alpina, de um ano de idade, pertencente ao Setor de Caprinocultura da Universidade Federal da Paraíba ­ Bananeiras - Brasil. Ambas as glândulas foram naturalmente infectadas por Staphylococcus simulans α-hemolítico. As glândulas mamárias apresentaram mastite aguda catarral com envolvimento sistêmico, respondendo positivamente ao tratamento sistêmico com gentamicina associada a amoxicilina. O presente relato sugere a importância do potencial patogênico de Staphylococcus não-aureus (SNA) como causador de mastite clínica também em animais nulíparos. O isolado mostrou resistência a tetraciclina e continha genes de produção de toxinas estafilocócicas (sec, seg e TSST-1). Além disso, tem sido relatado que Staphylococcus simulans é um patógeno emergente em seres humanos causando infecções cutâneas e osteoarticulares, principalmente naqueles que têm contato íntimo com animais de fazenda. Até onde sabemos, este é o primeiro relato de uma mastite clínica em uma cabra nulípara causada por Staphylococcus simulans.(AU)

Catarrhal mastitis by Staphylococcus simulans in a nulliparous goat / Mastite catarral causada por Staphylococcus simulans em uma cabra nulipara

The present paper is a case report of a one-year old nulliparous Alpine Goat belonging to a dairy goat farm in semi-arid region of Brazil. Both glands were naturally infected by α-hemolytic Staphylococcus simulans and evolved similar clinical signs. The mammary glands presented an acute catarrhal mastitis with systemic clinical signs that responded positively to treatment with gentamicin associated with amoxicillin. The present report suggests the importance of the pathogenic potential of non-aureus Staphylococci strains (NAS) as a cause of clinical mastitis also in nulliparous animals. The isolate showed resistance to tetracycline and contained staphylococcal toxin production genes (sec, sec and TSST-1). Moreover, it has been reported that Staphylococcus simulans is an emerging pathogen in humans causing cutaneous and osteoarticular infections, mainly in those in close contact with farm animals. To the best of our knowledge, this is the first report of a clinical mastitis in a nulliparous goat caused by Staphylococcus simulans(AU)

O presente trabalho é o relato do caso de uma cabra nulípara da raça Parda Alpina, de um ano de idade, pertencente ao Setor de Caprinocultura da Universidade Federal da Paraíba ­ Bananeiras - Brasil. Ambas as glândulas foram naturalmente infectadas por Staphylococcus simulans α-hemolítico. As glândulas mamárias apresentaram mastite aguda catarral com envolvimento sistêmico, respondendo positivamente ao tratamento sistêmico com gentamicina associada a amoxicilina. O presente relato sugere a importância do potencial patogênico de Staphylococcus não-aureus (SNA) como causador de mastite clínica também em animais nulíparos. O isolado mostrou resistência a tetraciclina e continha genes de produção de toxinas estafilocócicas (sec, seg e TSST-1). Além disso, tem sido relatado que Staphylococcus simulans é um patógeno emergente em seres humanos causando infecções cutâneas e osteoarticulares, principalmente naqueles que têm contato íntimo com animais de fazenda. Até onde sabemos, este é o primeiro relato de uma mastite clínica em uma cabra nulípara causada por Staphylococcus simulans.(AU)