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1.
Public Health Nurs ; 41(4): 815-824, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38683059

RESUMO

INTRODUCTION: Social networking sites perpetuate gender stereotypes, inequalities, and gender violence, generating adverse health effects. METHODS: This study aimed to analyze the relationship between Instagram, alcohol consumption and effects on health, through interviews and discussion groups involving adolescents (high school or university students and workers), during the period from the third to the sixth wave of the COVID-19 pandemic. RESULTS: A total of 118 individuals participated in the study. Three coding clusters were identified: (1) Inequality in the representation of men and women on SNSs; (2) Violence towards women's bodies and sexual violence on SNSs; and (3) Health problems derived from violence against women's body image on SNSs. The main focus of our analysis was the exhibition of the female body, in which eroticization of the body prevails and gender roles are perpetuated. Violence is generated towards women in the form of misogynistic attacks for daring to challenge conventional stereotypes. Many interviewees coincide that the main health effects include low self-esteem, anxiety and eating disorders. CONCLUSIONS: The institutional response to the abuses and violence committed on SNSs against women is weak, and public policies are needed to protect women in these environments where they are especially vulnerable.


Assuntos
Imagem Corporal , COVID-19 , Humanos , Feminino , Imagem Corporal/psicologia , Adolescente , COVID-19/psicologia , Adulto Jovem , SARS-CoV-2 , Rede Social , Adulto , Mídias Sociais , Masculino , Violência/psicologia
2.
Women Health ; 62(9-10): 753-763, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36414568

RESUMO

Immigrant women face a double vulnerability, being a woman and an immigrant, a situation that hinders their entry into the labor market and worsens their working conditions and occupational health with respect to the native population. The objective of these women is to seek employment or educational opportunities and improving their own lives and those of their families. All this is affected by the axes of gender, ethnicity, migration and socioeconomic level, as well as being confronted with the patriarchal structure of exercise of power and domination. These situations of inequality have aggravated due to the COVID-19 pandemic, worsening working conditions and their occupational health. This study aims to explore the working conditions of the female Brazilian immigrant population living in Porto (Portugal) and how these conditions may affect their health. Qualitative research through semi-structured interviews conducted during the second wave of the COVID-19 pandemic was used. The results show of them had work overload, manifesting anxiety and stress. Physical affectations related to poor work ergonomics and the lack of occupational health examinations in working immigrant women is highlighted. The importance of strengthening migration policies related to occupational health is highlighted. In pandemic situations, the vulnerability of these women increases, worsening their overall health.


Assuntos
COVID-19 , Emigrantes e Imigrantes , Feminino , Humanos , COVID-19/epidemiologia , Pandemias , Brasil/epidemiologia , Pesquisa Qualitativa
3.
Healthcare (Basel) ; 12(7)2024 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-38610181

RESUMO

BACKGROUND: Dating violence has become a problem of social relevance with short- and long-term health consequences. Nurses are in a privileged position to detect and address this problem in health facilities and as school nurses in schools, providing health education and detecting this violence correctly. AIM: The aim of this study was to evaluate the cross-cultural validation of the Portuguese version of the Multidimensional Scale of Dating Violence-Short (MSDV 2.0). METHODS: A validation investigation was carried out in two phases: (1) cross-cultural adaptation of the items and content validation of the Portuguese version of MSDV 2.0 and (2) psychometric validation. RESULTS: Phase (1): The items of the original version include a cross-cultural translation from Spanish to Portuguese and analysed by a group of experts in gender violence and by the authors of the original scale, then a back translation was made and again reviewed by the experts. Young university students also participated for face validity, and a pilot test was carried out. Phase (2): Confirmatory factor analysis was performed using the robust maximum-likelihood estimation method, which confirmed the five-dimensional structure, obtaining good fit rates (chi-square significance (χ2) = 187.860 (p < 0.0001); root mean square error of approximation (RMSEA) = 0.049; comparative fit index (CFI) = 0.937; Tucker-Lewis index (TLI) = 0.923). Reliability analysis indicated adequate internal consistency (Cronbach's alpha (α) = 0.88 to 0.70). Finally, scores of the Portuguese versions MSDV 2.0 were correlated, as expected, positively with the Depression, Anxiety, and Stress Scale (DASS-21) (r = 0.36 to 0.16) and negatively with the Medical Outcomes Study Questionnaire Short Form 36, Health Survey (SF-36) (r = -0.30 to -0.14). CONCLUSIONS: To date, it is the only instrument that measures dating violence in a multidimensional way validated in the Portuguese university context.

4.
Enferm Infecc Microbiol Clin ; 30(3): 131-6, 2012 Mar.
Artigo em Espanhol | MEDLINE | ID: mdl-22119095

RESUMO

INTRODUCTION: The number of children of immigrant origin in the last few years has increased the cohort of HIV-infected children in the Community of Madrid. The objectives of the study were to evaluate the epidemiological and clinical characteristics of the new diagnosed children and describe the different subtypes of HIV-1. PATIENTS AND METHODS: The new diagnosed children were analysed from the year 1997, divided into 3 periods: P1 (1997-2000), P2 (2001-2004), P3 (2005-2009). The regions and countries of origin, the clinical, immune and viral characteristics, as well as the response to treatment were analysed. The subtypes of HIV-1 were evaluated by phylogenetic analysis of protease genes and reverse transcriptase. RESULTS: We identified 141 new diagnoses of HIV infection, the percentage of immigrant origin in P1 was (22.5%), P2 (50%) and P3 (68%). The origin had changed from Latin America in P1 to sub-Saharan Africa in P3. There were no differences between Spanish and immigrant children in the age at diagnosis, the CDC clinical stage A/B/C, viral load, percentage of CD4 at diagnosis and actual. Better viral response was more likely in immigrants after the first regimen of HAART (Highly active antiretroviral treatment) independently of the treatment received. A total of 66 subtypes were obtained, 24% were subtypes non-B (56% recombinants forms). All subtypes of Spanish children (43) and Latin American (5) were subtypes B, and all the children from sub-Saharan Africa (14) were subtypes non-B. CONCLUSION: There were no differences between immigrants and Spanish children infected by HIV, except the different subtypes of HIV-1.


Assuntos
Emigrantes e Imigrantes/estatística & dados numéricos , Infecções por HIV/epidemiologia , HIV-1/isolamento & purificação , Adolescente , África Subsaariana/etnologia , Contagem de Linfócito CD4 , Criança , Pré-Escolar , Feminino , Infecções por HIV/transmissão , Infecções por HIV/virologia , Protease de HIV/genética , Transcriptase Reversa do HIV/genética , HIV-1/classificação , HIV-1/genética , Humanos , Lactente , Transmissão Vertical de Doenças Infecciosas , América Latina/etnologia , Masculino , Morbidade/tendências , Filogenia , Estudos Prospectivos , Espanha/epidemiologia , Carga Viral , Viremia/epidemiologia , Viremia/virologia , Adulto Jovem
5.
Artigo em Inglês | MEDLINE | ID: mdl-33573237

RESUMO

This article analyzes health mediation among equals as an educational strategy against risk behaviors in young people from both the educational and associative systems in Seville (Spain), based on qualitative research, with the aim of assessing and comparing its impact in those areas. To this end, interviews with 49 professionals and 427 adolescents were conducted in discussion groups. Results acknowledge mediation as individual or group intervention accepted by young people as advice and information on health issues and conflict resolution, but also as a method for data collection in order to obtain a community health diagnosis. The educational system implements this strategy, but in associations it seems to work better, particularly in the psycho-emotional and sexual spheres. Unfortunately, intervention programs are usually discontinuous due to lack of resources and territorial variability. And this is why mediators' support is highly valued by the target users, with preference for a male figure in the case of boys, and larger predisposition towards a female mediator in girls, except in cases where this agent has a special social relevance.


Assuntos
Negociação , Comportamento Sexual , Adolescente , Feminino , Humanos , Masculino , Pesquisa Qualitativa , Assunção de Riscos , Espanha
6.
Artigo em Inglês | MEDLINE | ID: mdl-34300091

RESUMO

This study aims to identify acculturation experiences about social relations and health behaviors of first-generation Chinese immigrants in the South of Spain, including food patterns, physical exercise, and tobacco and alcohol use. A phenomenological qualitative study was conducted using semi-structured interviews, informal conversations, and field notes. All data were analyzed under the Berry's Model of Acculturation. A total of 133 Chinese immigrants were included. Our findings show that separation was the dominant acculturation strategy, followed by integration and assimilation, while marginalization was not present in this immigrant population. Most of the immigrant population maintains a link to the customs of their home country, favoring the process of identity and collective self-esteem. These results can help health managers and the government to further understand Chinese immigrants in Europe and to establish appropriate health interventions to this group.


Assuntos
Aculturação , Emigrantes e Imigrantes , China , Europa (Continente) , Comportamentos Relacionados com a Saúde , Humanos , Espanha
7.
Nutrients ; 13(3)2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33652952

RESUMO

This article aims to explore the food patterns of Chinese immigrants living in Spain and to understand the factors associated with these behaviors. This qualitative ethnographic study included 133 Chinese immigrants; using interviews with scripts were based on the National Health Survey of Spain. Three categories were defined: "Differences between Chinese food and Western food"; "Products and dishes consumed by Chinese immigrants" and "Modification of eating habits". Participants found a remarkable difference between eastern (i.e., vegetables and rice) and western (i.e., dairy, high-fat, bread) foods. They also experienced a change in their eating habits, mostly related to acculturation and lack of time. Chinese immigrants have different eating patterns as compared to the native population in Spain and this information could help in future healthcare strategies. Knowing the food culture could infer aspects, such as insertion or integration, and promoting health and well-being.


Assuntos
Povo Asiático/psicologia , Dieta/etnologia , Emigrantes e Imigrantes/psicologia , Comportamento Alimentar/etnologia , Aculturação , Adulto , Antropologia Cultural , China/etnologia , Feminino , Humanos , Masculino , Pesquisa Qualitativa , Espanha
8.
AIDS ; 35(3): 393-398, 2021 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33252487

RESUMO

OBJECTIVE: The source of residual HIV viremia is highly debated and its potential relationship with the HIV reservoir has not been clarified. Herein, we analysed the cell-associated HIV-DNA content in two important cell compartments of the HIV reservoir, resting CD4+ T memory (Trm) and peripheral T follicular helper (pTfh) cells, and the association with the residual HIV viremia in individuals with spontaneous HIV replication control (elite controllers, EC group) and in individuals with antiretroviral therapy (ART)-mediated HIV replication control (cART group). DESIGN: A cross-sectional study. METHODS: Seventeen chronically HIV-infected patients with suppressed HIV replication were included. Cell-associated HIV-DNA was measured by ultrasensitive digital-droplet-PCR in purified Trm and pTfh cells. Residual HIV plasma viremia was quantified using a single-copy assay with a sensitivity of 0.3 HIV-RNA copies/ml. RESULTS: A significant and positive correlation was demonstrated between HIV-DNA levels in pTfh cells and residual plasma viral load (rpVL) (rho = 0.928, P = 0.008) in HIV-positive elite controllers, but not in HIV-positive treated patients, despite the lower levels of cell-associated HIV-DNA found in elite controllers compared with cART patients in pTfh cells [176 (77-882) vs. 608 (361-860) copies/million cells, respectively; P = 0.05]. CONCLUSION: This association suggests that pTfh cells could have an important contribution to persistent viremia in elite controllers. This could be the consequence of a more limited control of HIV replication in elite controllers with higher transcriptional activity of HIV in pTfh cells of elite controllers than that in cART patients.


Assuntos
Infecções por HIV , HIV-1 , Contagem de Linfócito CD4 , Linfócitos T CD4-Positivos , Estudos Transversais , DNA , Infecções por HIV/tratamento farmacológico , HIV-1/genética , Humanos , Carga Viral , Viremia
9.
Nutrients ; 13(4)2021 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-33808476

RESUMO

OBJECTIVE: The health effects of a supplemented Mediterranean diet (SMD) with extra-virgin olive oil (EVOO) and nuts are well documented in non-HIV-infected individuals. We hypothesised that the benefits of an SMD could be mediated by changes in the gut microbiota, even in those with an altered intestinal microbiota such as people living with HIV. DESIGN: Individuals living with HIV (n = 102) were randomised to receive an SMD with 50 g/day of EVOO and 30 g/day of walnuts (SMD group) or continue with their regular diet (control group) for 12 weeks. METHODS: Adherence to the Mediterranean diet was assessed using the validated 14-item MD-Adherence-Screener (MEDAS) from the PREDIMED study. A sub-study classifying the participants according to their MEDAS scores was performed. RESULTS: The lipid profile was improved in the SMD group vs. that in the control group (delta-total cholesterol and delta-B-lipoprotein). The immune activation (CD4+HLADR+CD38+ and CD8+HLADR+CD38+ cells) and IFN-γ-producing T-cells significantly decreased at week 12 compared to the baseline in the SMD group but not in the control group. The gut microbiota in those from the high-adherence group presented significantly high diversity and richness at the end of the intervention. Succinivibrio and Bifidobacterium abundances were influenced by the adherence to the MD and significantly correlated with Treg cells. CONCLUSION: The Mediterranean diet improved metabolic parameters, immune activation, Treg function, and the gut microbiota composition in HIV-1-infected individuals. Further, Mediterranean diet increased the Bifidobacterium abundances after the intervention, and it was associated to a beneficial profile.


Assuntos
Dieta Mediterrânea , Microbioma Gastrointestinal/efeitos dos fármacos , Infecções por HIV/dietoterapia , HIV-1 , Adulto , Translocação Bacteriana , Bifidobacterium , Biomarcadores/sangue , Feminino , Humanos , Inflamação/sangue , Inflamação/tratamento farmacológico , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Nozes , Azeite de Oliva , Cooperação do Paciente , Succinivibrionaceae , Subpopulações de Linfócitos T
10.
Artigo em Inglês | MEDLINE | ID: mdl-32764456

RESUMO

Adolescence and youth are stages of exploration and experimentation, when the consumption of psychoactive substances for recreational or experimental purposes often begins. The general objective of this study was to explore youth consumption habits in nightlife settings and associated factors in Andalusia (Spain). To this end, we took into account young people's perceptions about patterns of drug polyconsumption in nightlife settings and the perceptions and actions of health and teaching professionals towards this issue. We carried out a qualitative methodology with 24 in-depth interviews and 3 discussion groups with Andalusian girls and boys aged between 16 and 22 (n = 45) and 13 in-depth interviews with social agents (health and teaching professionals). We performed narrative discourse analysis and triangulation of identified categories and measured the units of analysis. The results show information relating to gender, age of initiation, most commonly consumed substances, motivation and effects, peer group pressure and how they obtained the substances, and the perceptions held and main activities carried out in the educational institutions and health centers.


Assuntos
Motivação , Transtornos Relacionados ao Uso de Substâncias , Adolescente , Adulto , Fatores Etários , Feminino , Humanos , Masculino , Grupo Associado , Recreação , Fatores Sexuais , Espanha , Transtornos Relacionados ao Uso de Substâncias/epidemiologia , Adulto Jovem
11.
Artigo em Inglês | MEDLINE | ID: mdl-33113817

RESUMO

In general, immigrants suffer poor working conditions. This is particularly true in the case of women, who constitute 48% of international migrants, and these poor conditions are closely linked to the sectors they mainly occupy, such as domestic and care-giving services. The aim of the present study was to investigate the working conditions of the female immigrant population living in southern Spain and how these conditions may affect their health. A qualitative study using semi-structured interviews and discussion groups was conducted over one year in 2019, with 61 immigrant women recruited. The sectors occupied by immigrant women were caregiving for dependent people and domestic services. Most of the female immigrants interviewed were working (63.94%), although the majority were employed in an irregular situation, with a very long working day. Among the main risks identified were biological risks, physical attacks, falls, wounds and musculoskeletal complaints related to handling patients and carrying out household chores. Most of them had not taken an occupational health test and did not report accidents occurring in the workplace for fear of losing their jobs. The main health problems were related to physical and mental health (such as musculoskeletal diseases and stress). These findings highlight the importance of making a major change in our perspective regarding the social value of including immigrant women in the labour market and the different aspects related to their health.


Assuntos
Cuidadores/psicologia , Emigrantes e Imigrantes , Saúde Ocupacional , Adulto , Feminino , Humanos , Entrevistas como Assunto , Pesquisa Qualitativa , Serviço Social , Espanha/epidemiologia , Local de Trabalho
12.
Sci Rep ; 8(1): 15541, 2018 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-30341387

RESUMO

A recent study has pointed out to CD32a as a potential biomarker of HIV-persistent CD4 cells. We have characterized the level and phenotype of CD32+ cells contained in different subsets of CD4 T-cells and its potential correlation with level of total HIV-DNA in thirty HIV patients (10 typical progressors naïve for cART, 10 cART-suppressed patients, and 10 elite controllers). Total HIV-DNA was quantified in different subsets of CD4 T-cells: Trm and pTfh cells. Level and immunephenotype of CD32+ cells were analyzed in these same subsets by flow cytometry. CD32 expression in Trm and pTfh subsets was similar in the different groups, and there was no significant correlation between the level of total HIV-DNA and the level of CD32 expression in these subsets. However, total HIV-DNA level was correlated with expression of CD127 (rho = -0.46, p = 0.043) and of CCR6 (rho = -0.418, p = 0.027) on CD32+ cells. Our results do not support CD32 as a biomarker of total HIV-DNA content. However, analyzing the expression of certain markers by CD32+ cells could improve the utility of this marker in the clinical setting, prompting the necessity of further studies to both validate our results and to explore the potential utility of certain markers expressed by CD32+ cells.


Assuntos
Antirretrovirais/uso terapêutico , Linfócitos T CD4-Positivos/virologia , DNA Viral/análise , Expressão Gênica , Infecções por HIV/patologia , HIV/crescimento & desenvolvimento , Receptores de IgG/análise , Adulto , Linfócitos T CD4-Positivos/química , Feminino , Citometria de Fluxo , Perfilação da Expressão Gênica , Infecções por HIV/tratamento farmacológico , Infecções por HIV/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Subpopulações de Linfócitos T/química , Subpopulações de Linfócitos T/virologia , Carga Viral
13.
Pediatr Infect Dis J ; 26(4): 334-8, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17414398

RESUMO

BACKGROUND: There are few data on long-term effects of highly active antiretroviral therapy (HAART) on weight and height in HIV-infected children. OBJECTIVES: Our objective was to assess the effect of HAART on the weight and height of HIV-infected children over time in the Madrid cohort, and analyze possible factors associated with the effect. PATIENTS AND METHODS: This was a retrospective study of HIV-infected children starting HAART in 1997 or later. Serial measurements of weight, height and body mass index (BMI) were performed and converted to z-scores using the Spanish revised reference data. Changes from baseline in weight, height and BMI at 12, 24, 36, 48 and 60 months were determined. Associations of z-scores at the last visit with immunologic (CD4% above 25%) and virologic responses (more than 50% of samples below 400 copies/mL), CDC (Centers for Disease Control) clinical category, and the presence and type of lipodystrophy (lipoatrophy or lipohypertrophy) were evaluated. RESULTS: Twelve hundred and twelve children, 97% of them vertically-infected, received HAART starting in 1997 for a median of 71 months (4-102 months). Median age at initiation of HAART was 6 years (1 month-18 years). Thirty-nine percentage were antiretroviral naive and 61% had received NRTI therapy previously. Thirty-two percentage and 53% had CDC class C and immunologic class 3, respectively. At the final evaluation, 24% of children remained on their first combination therapy, 39% on the second and 37% had received at least 3 different HAART regimens. Fifty-one percentage were classified as virologic responders. Thirty-nine percentage of children in this study were diagnosed with lipodystrophy. At baseline, median z-score for weight, height and BMI were -0.45, -0.60 and -0.33, respectively. HAART was associated with significant increases in z-scores of weight and height but not BMI at the different time-points analyzed. Virologic nonresponders had significantly lower z-scores for weight and height but not for BMI. CDC class C was associated with lower z-scores for height. No differences in final measurements were observed for baseline CD4, immunologic response or lipoatrophy. Children with lipohypertophy had a significantly higher BMI at the last visit. CONCLUSIONS: HIV-infected children experienced a continued catch-up in weight and height 5 years after starting HAART. Virologic control is related to sustained growth.


Assuntos
Fármacos Anti-HIV/uso terapêutico , Terapia Antirretroviral de Alta Atividade , Estatura/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Infecções por HIV/tratamento farmacológico , Adolescente , Índice de Massa Corporal , Criança , Pré-Escolar , Feminino , Infecções por HIV/imunologia , Infecções por HIV/fisiopatologia , Humanos , Lactente , Masculino , Resultado do Tratamento
14.
Rev Esp Geriatr Gerontol ; 52(2): 80-86, 2017.
Artigo em Espanhol | MEDLINE | ID: mdl-27126264

RESUMO

INTRODUCTION: The aim of this study was to identify the physical determining factors of the Fear of Falling Syndrome (FoF) in older adults with a history of falls. METHODS: An observational study was conducted on 183 subjects older than 64 years with a fall in the previous year, with data collected from the geriatrics outpatient clinic of the Complejo Hospitalario Universitario from Albacete, Spain. Sociodemographic and anthropometric data, as well as comorbidity, drugs usually taken, functional status, physical function, frailty, cognitive and affective status were collected. Muscle mass was measured using bioimpedancy meter (BIA), and densitometry (DXA), strength with digital hand-held JAMAR dynamometer and with a Leg-press machine, muscle potency with a T-Force instrument, gait variability with the Gait-Rite instrument, and postural stability with the Neurocom Balance Master posturograph were also determined. An analysis was performed to determine if the FoF is associated with physical impairments adjusted for the study covariates. RESULTS: The study included 140 subjects with FoF, and 43 without it. The mean age was 78.4 years, and 147 were women. Posturography could be measured in 182 participants, DXA in 117, BIA in 165, and muscle potency in 146. FoF was associated with female sex, frailty, depressed mood, social risk, muscle strength and power, physical function, number of drugs used, and orthostatic hypotension in the overall sample. After adjusting for sex, only frailty, depressed mood, and number of drugs remained associated. CONCLUSIONS: Rationale, design, and methods of the FISTAC study are presented.


Assuntos
Acidentes por Quedas , Medo , Avaliação Geriátrica , Idoso , Feminino , Humanos , Masculino , Síndrome
15.
J Proteomics ; 150: 109-120, 2017 01 06.
Artigo em Inglês | MEDLINE | ID: mdl-27620695

RESUMO

Analytical proteomics has experienced exponential progress in the last decade and can be expected to lead research studies on diagnostic and therapeutic biomarkers in the near future. Because the development of this type of analysis requires the use of a large number of human samples with a minimum of quality requirements, our objective was to identify appropriate indicators for quality control of plasma samples stored in biobanks for research in proteomics. To accomplish this, plasma samples from 100 healthy donors were obtained and processed according to the pre-analytical variables of: a) time delay for the first centrifugation of the original blood sample (4 or 24h) and b) number of freeze/thaw cycles (1, 2 or 3) of the processed plasma samples. The analyses of samples were performed by different and complementary methods such as SPE MALDI-TOF, DIGE, shotgun (iTRAQ, nLC MALDI TOF/TOF) and targeted nLC MS/MS proteomic techniques (SRM). In general, because the distribution of proteins in all samples was found to be very similar, the results shown that delayed processing of blood samples and the number of freeze/thaw cycles has little or no effect on the integrity of proteins in the plasma samples. SIGNIFICANCE: The results of the present work indicate that blood proteins in plasma are broadly insensitive to such preanalytical variables as delayed processing or freeze/thaw cycles when analyzed at the peptide level. Although there are other studies related to protein stability of clinical samples with similar results, what is remarkable about our work is the large number of plasma samples examined and that our analyses assessed protein integrity by combining a wide set of complementary proteomic approaches performed at different proteomic platform participating laboratories that all yielded similar results. We believe our study is the most comprehensive performed to date to determine the changes in proteins induced by delayed sample processing and plasma freeze/thaw cycles.


Assuntos
Bancos de Espécimes Biológicos/normas , Preservação de Sangue/normas , Coleta de Amostras Sanguíneas/normas , Proteômica/métodos , Controle de Qualidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Preservação de Sangue/métodos , Coleta de Amostras Sanguíneas/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estabilidade Proteica , Manejo de Espécimes/métodos , Manejo de Espécimes/normas , Adulto Jovem
17.
J Acquir Immune Defic Syndr ; 64(2): 149-53, 2013 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-24047967

RESUMO

INTRODUCTION: Microbial translocation (MT) has been proposed as one of the triggering mechanisms of persistent immune activation associated to HIV-1 infection. Our objectives were to determine the correlation between different measurements of MT in suppressed HIV-1-infected individuals and to evaluate its correlation with immune activation. METHODS: Eighteen suppressed HIV-1-infected patients with CD4+ T-cell count above 350 cells per cubic millimeter and undetectable plasma viral load, included in antiretroviral treatment intensification clinical trials, were evaluated. Samples obtained at baseline and at established time points during the trials were analyzed. Lipopolysaccharide (LPS), lipopolysaccharide binding protein (LBP), soluble CD14 (sCD14), and bacterial 16S ribosomal DNA (16S rDNA), and markers of immune activation were determined. RESULTS: We analyzed 126 plasma samples from the 18 patients. LPS significantly correlated with sCD14 (P < 0.001, r = 0.407) and LBP (P = 0.042, r = 0.260). Also, a significant correlation was found between sCD14 and LBP (P = 0.009, r = 0.325) but not between bacterial 16S rDNA and LPS, sCD14, or LBP (P = 0.346, P = 0.405, and P = 0.644). On the other hand, no significant correlation was found between LPS, sCD14, or LBP and CD4 (P = 0.418, P = 0.619, and P = 0.728) or CD8 T-cell activation (P = 0.352, P = 0.275, and P = 0.124). Bacterial 16S rDNA correlated with activated CD4 T cells (P = 0.005, r = 0.104) but not with activated CD8 T cells (P = 0.171). CONCLUSIONS: There is a good correlation in the quantification of LPS, sCD14, and LBP levels, but not with bacterial 16S rDNA, as measurements of MT. We are unable to ensure that MT directly triggers T-cell immune activation at least among these patients with relatively good immune recovery and under treatment intensification.


Assuntos
Translocação Bacteriana , DNA Bacteriano/análise , Infecções por HIV/imunologia , HIV-1/imunologia , Receptores de Lipopolissacarídeos/sangue , Lipopolissacarídeos/sangue , Ativação Linfocitária/imunologia , Proteínas de Fase Aguda , Adulto , Fármacos Anti-HIV/uso terapêutico , Translocação Bacteriana/genética , Translocação Bacteriana/imunologia , Contagem de Linfócito CD4 , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/virologia , Proteínas de Transporte/sangue , DNA Bacteriano/genética , DNA Ribossômico/análise , Feminino , Infecções por HIV/tratamento farmacológico , Infecções por HIV/virologia , HIV-1/fisiologia , Humanos , Receptores de Lipopolissacarídeos/imunologia , Lipopolissacarídeos/imunologia , Masculino , Glicoproteínas de Membrana/sangue , Pessoa de Meia-Idade , RNA Ribossômico 16S/genética , Fatores de Tempo , Resultado do Tratamento , Carga Viral
18.
Rev. esp. geriatr. gerontol. (Ed. impr.) ; 52(2): 80-86, mar.-abr. 2017. tab
Artigo em Espanhol | IBECS (Espanha) | ID: ibc-160802

RESUMO

Introducción. El objetivo del estudio es identificar los atributos físicos del síndrome de temor a caerse (STAC) en mayores con caídas previas. Metodología. Estudio observacional analítico sobre 183 sujetos mayores de 64 años que hayan sufrido al menos una caída en el último año, captados desde la consulta de geriatría del Complejo Hospitalario Universitario de Albacete. De ellos, 140 cumplían criterios de STAC, y los otros 43 no (grupo control). Como covariables se recogieron datos sociodemográficos, antropométricos, de comorbilidad y fármacos, situación funcional, función física, fragilidad, estado cognitivo y afectivo. Se determinó la masa muscular mediante bioimpedanciometría (BIA) y densitometría (DXA), la fuerza prensora mediante dinamómetro digital de JAMAR, la fuerza extensora (1RM) de miembros inferiores, la potencia muscular de miembros inferiores mediante el instrumento T-Force, la variabilidad de la marcha con el instrumento Gait-Rite, las alteraciones posturales mediante posturografía. Se analizará si el STAC se asocia con alteraciones físicas ajustado por las covariables de estudio. Resultados. Edad media 78,4 años, 147 mujeres. En 182 participantes se pudo realizar posturografía, en 146 se pudo determinar potencia muscular de piernas, en 117 se realizó DXA y en 165, BIA. El STAC se asoció a sexo femenino, estado de fragilidad, ánimo deprimido, riesgo social, fuerza y potencia muscular, función física, número de fármacos e hipotensión ortostática en la muestra global, pero al ajustar por sexo, solo la fragilidad, el ánimo deprimido y el consumo de fármacos se asociaron al STAC. Conclusiones. Se presenta el razonamiento, el diseño y la metodología del estudio FISTAC (AU)


Introduction. The aim of this study was to identify the physical determining factors of the Fear of Falling Syndrome (FoF) in older adults with a history of falls. Methods. An observational study was conducted on 183 subjects older than 64 years with a fall in the previous year, with data collected from the geriatrics outpatient clinic of the Complejo Hospitalario Universitario from Albacete, Spain. Sociodemographic and anthropometric data, as well as comorbidity, drugs usually taken, functional status, physical function, frailty, cognitive and affective status were collected. Muscle mass was measured using bioimpedancy meter (BIA), and densitometry (DXA), strength with digital hand-held JAMAR dynamometer and with a Leg-press machine, muscle potency with a T-Force instrument, gait variability with the Gait-Rite instrument, and postural stability with the Neurocom Balance Master posturograph were also determined. An analysis was performed to determine if the FoF is associated with physical impairments adjusted for the study covariates. Results. The study included 140 subjects with FoF, and 43 without it. The mean age was 78.4 years, and 147 were women. Posturography could be measured in 182 participants, DXA in 117, BIA in 165, and muscle potency in 146. FoF was associated with female sex, frailty, depressed mood, social risk, muscle strength and power, physical function, number of drugs used, and orthostatic hypotension in the overall sample. After adjusting for sex, only frailty, depressed mood, and number of drugs remained associated. Conclusions. Rationale, design, and methods of the FISTAC study are presented (AU)


Assuntos
Humanos , Masculino , Feminino , Idoso , Idoso de 80 Anos ou mais , Medo/psicologia , Transtornos Fóbicos/epidemiologia , Transtornos Fóbicos/psicologia , Sarcopenia/complicações , Sarcopenia/psicologia , Síncope/psicologia , Transtornos Cognitivos/complicações , Transtornos Cognitivos/psicologia , Atividade Motora/fisiologia , Antropometria , Comorbidade , Envelhecimento Cognitivo/psicologia , Densitometria/métodos , Repertório de Barthel
19.
Acta méd. colomb ; 40(2): 166-168, abr.-jun. 2015. ilus, tab
Artigo em Espanhol | LILACS, COLNAL | ID: lil-762705

RESUMO

La afectación osteoarticular es la complicación más frecuente de la brucelosis. La espondilodiscitis es una complicación grave, ya que suele estar asociada con la formación de abscesos. Se presenta el caso de un varón de 43 años de edad, natural de Marruecos, con síntomas, signos y hallazgos imagenológicos de espondilodiscitis de larga evolución, orquitis y endocarditis. Mediante la realización de pruebas de laboratorio complementarias se llega al diagnóstico de infección por Brucella spp. El presente caso busca concientizar a la comunidad médica sobre la importancia de considerar la infección por Brucella spp. como diagnóstico diferencial en pacientes con sospecha de espondilodiscitis infecciosa, especialmente en aquellos pacientes con antecedentes epidemiológicos, permitiendo así realizar un acertado y oportuno diagnóstico. Este caso resulta interesante porque nunca antes se había descrito la presencia simultánea de estas tres manifestaciones de la brucelosis en un paciente inmuno competente. (Acta Med Colomb 2015; 40: 166-168).


Osteoarticular involvement is the most common complication of brucellosis. Spondylodiscitis is a serious complication as it is often associated with abscess formation. The case of a 43 years old man born in Morocco with symptoms, signs and imaging findings of longstanding spondylodiscitis, orchitis and endocarditis is presented. Through the implementation of complementary laboratory tests, diagnosis infection by Brucella spp. was made.This case aims to raise awareness in the medical community about the importance of considering Brucella spp. as a differential diagnosis in patients with suspected infectious spondylodiscitis, especially in those patients with epidemiological background, allowing make a successful and timely diagnosis. This case is interesting because never before had been described the simultaneous presence of these three manifestations of brucellosis in an immunocompetent patient. (Acta Med Colomb 2015; 40: 166-168).


Assuntos
Humanos , Masculino , Adulto , Discite , Orquite , Brucella , Brucelose , Diagnóstico Diferencial , Endocardite
20.
Texto & contexto enferm ; 23(1): 126-133, Jan-Mar/2014. graf
Artigo em Inglês | BDENF - enfermagem (Brasil), LILACS | ID: lil-705901

RESUMO

This is a quantitative, cross-sectional, descriptive study, conducted in two consecutive series in 2007 (S1) and 2008 (S2), comprised of 350 people between 15 and 29 years of age, with the aim to understand the profile of adolescents and young adults from the hip hop culture of Seville, Spain, and to detect risky sexual behavior in their everyday lives. Data were obtained by means of a self-administered questionnaire followed by a statistical analysis, corresponding to a sexually active population (91%), who initiated their sexual experiences before 16.5 years of age. Most state they are heterosexual and use contraception. In conclusion, the image of this group is tied to virile masculine stereotypes, constituting the imaginary of this body of people. Direct interventions are needed to promote health and disease prevention, before adolescence, involving health services, families and schools, which address sexual education and love, taking into account the social construct and everyday life.


Estudo quantitativo descritivo transversal, realizado em duas séries consecutivas, correspondendo a 2007 (S1) e 2008 (S2), abrangendo 350 pessoas, entre 15-29 anos, com objetivo de conhecer o perfil dos adolescentes e jovens da cultura hip hop em Sevilha, Espanha, detectando comportamentos sexuais de risco no cotidiano. Coletou-se dados através de questionário autoadministrado, com análise estatística. A população é sexualmente ativa (91%), iniciando suas experiências sexuais antes dos 16,5 anos. A maioria relata heterossexualidade e usa métodos contraceptivos. Conclui-se que a imagem deste coletivo liga-se a estereótipos masculinos viris, constituindo o imaginário e cotidiano do grupo. Faz-se necessário dirigir intervenções para promoção da saúde e prevenção de agravos, antes da adolescência, nos serviços de saúde, na família e escola, envolvendo educação sexual e amorosa, considerando o imaginário social e o cotidiano.


Estudio cuantitativo de corte transversal realizado en dos series consecutivas, correspondientes a 2007 (S1) y 2008 (S2), que comprende 350 personas, entre 15-29 años, con objetivo de conocer el perfil de los adolescentes y jóvenes en la cultura hip hop de Sevilla, España, detectar comportamientos sexuales de riesgo en la vida cotidiana. Los datos fueron recogidos a través de cuestionario autoaplicado con análisis estadístico. Población sexualmente activa (91%), iniciando sus experiencias sexuales antes de 16,5 años. La mayoría reportes heterosexualidad, usa anticonceptivos. Se concluye que la imagen de este colectivo se une a los estereotipos masculinos viriles, constituyendo el imaginario diario del grupo. Es necesario intervenciones directas para la promoción de la salud y prevención de enfermedades, antes de la adolescencia, de servicios de salud, la familia y la escuela, que implica la educación sexual y el amor, teniendo en cuenta el imaginario social y lo cotidiano.


Assuntos
Humanos , Adolescente , Infecções Sexualmente Transmissíveis , Adolescente , Sexualidade
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